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We aimed to determine the impact of INH mono-resistance on TB treatment outcome in the European Union/European Economic Area and to identify risk factors for unsuccessful outcome in cases with INH mono-resistant ... Treatment was successful in 74.0% of INH mono-resistant and 77.4% of susceptible TB cases. ... with INH mono-resistance. ...doi:10.2807/1560-7917.es.2019.24.12.1800392 pmid:30914081 pmcid:PMC6440580 fatcat:dnu6ifwixfh4rlgttr4lrk2m7i
AbstractSummaryA number of methods have been devised to address the need for targeted genomic resequencing. One of these methods, Region-specific extraction (RSE) of DNA is characterized by the capture of long DNA fragments (15-20 kb) by magnetic beads, after enzymatic extension of oligonucleotides hybridized to selected genomic regions. Facilitating the selection of the most optimal capture oligos targeting a region of interest, satisfying the properties of temperature (Tm) and entropy (ΔG),doi:10.1101/2019.12.12.873497 fatcat:nczdd5mq55bxrofvig6rj27kgq
more »... ile minimizing the formation of primer dimers in a pooled experiment is therefore necessary. Manual design and selection of oligos becomes an extremely arduous task complicated by factors such as length of the target region and number of targeted regions. Here we describe, AnthOligo, a web-based application developed to optimally automate the process of generation of oligo sequences to be used for the targeting and capturing the continuum of large and complex genomic regions. Apart from generating oligos for RSE, this program may have wider applications in the design of customizable internal oligos to be used as baits for gene panel analysis or even probes for large-scale comparative genomic hybridization (CGH) array processes.Implementation and AvailabilityThe application written in Java8 and run on Tomcat9 is a lightweight Java Spring MVC framework that provides the user with a simple interface to upload an input file in BED format and customize parameters for each task. A Redis-like MapReduce framework is implemented to run sub-tasks in parallel to optimize time and system resources alongside a 'task-queuing' system that runs submitted jobs as a server-side background daemon. The task of probe design in AnthOligo commences when a user uploads an input file and concludes with the generation of a result-set containing an optimal set of region-specific oligos.AnthOligo is currently available as a public web application with URL: http://antholigo.chop.edu.
Copyright © 2020 Yarmarkovich, Farrel, Sison, di Marco, Raman, Parris, Monos, Lee, Stevanovic and Maris. ...doi:10.3389/fimmu.2020.00069 pmid:32256484 pmcid:PMC7092187 fatcat:p367e3eprbfatewjim7wrlwcde
secretion following oral glucose tolerance test or prolonged fasting; none had protein-sensitive hypoglycemia. Linkage analysis mapped the HI locus to Chr10q21-22, a region containing 48 genes. Three novel noncoding variants were found in hexokinase 1 (HK1) and one missense variant in the coding region of DNA2 . Conclusion: Dominant, diazoxideresponsive HI in this family maps to a novel locus on Chr10q21-22. HK1 is the more attractive disease gene candidate since a mutation interfering with thedoi:10.1159/000351943 pmid:23859901 pmcid:PMC3876732 fatcat:vgeixyymonedhkxpt7mgops7qa
more »... normal suppression of HK1 expression in beta-cells could readily explain the hypoglycemia phenotype of this pedigree.
The EFSA Panel on Food Additives and Flavourings (FAF) provides a scientific opinion re-evaluating the safety of thermally oxidised soya bean oil interacted with mono- and diglycerides of fatty acids ( ... Groups of 120 rats of each sex fed a diet containing mono-and diglycerides served as controls. The diets given to all groups were isocaloric. ... It is produced by interaction and deodorisation under vacuum at 130°C of 10% of thermally oxidised soya bean oil and 90% mono-and diglycerides of food fatty acids. ...doi:10.2903/j.efsa.2018.5420 pmid:32625705 pmcid:PMC7009384 fatcat:f5pj57gycrdflfijw3dmkcwgzq
background: The methodologies for assessment of anti-human leukocyte (anti-HLA) antibodies in heart transplantation (HT) have evolved, with sensitive flow cytometry largely replacing cytotoxicity techniques. The impact of these changes on post-HT outcomes, however, is uncertain. We sought to determine trends in pre-HT anti-HLA antibody assessment and whether the methodology of assessment identifies patients at higher risk of graft loss.doi:10.1016/s0735-1097(14)60722-1 fatcat:fw4o62nh5vd2namdbg3q3jnqha
Acquired aplastic anemia (aAA) is a non-malignant disease caused by autoimmune destruction of early hematopoietic cells. Clonal hematopoiesis is a late complication, seen in 20-25% of older patients. We hypothesized that clonal hematopoiesis in aAA is a more general phenomenon, which can arise early in disease even in younger patients. To evaluate clonal hematopoiesis in aAA, we used comparative whole exome sequencing of paired bone marrow and skin in 22 patients. We found somatic mutations indoi:10.1016/j.cancergen.2015.01.007 pmid:25800665 pmcid:PMC4433860 fatcat:xueobkgfgjeyhc46hscozxucc4
more »... ixteen patients (72.7%) with a median disease duration of 1 year; twelve (66.7%) were patients with pediatriconset aAA. Fifty-eight mutations in 51 unique genes
Unrelated donor hematopoietic stem cell transplantation (HSCT) is increasingly being used to cure nonmalignant hematologic diseases (NMHD) in patients who lack HLA matched related donors. Both graft rejection and graft-versus-host disease (GVHD) remain major barriers to safe and effective transplant for these patients requiring unrelated donors. Partial T cell depletion combined with peripheral stem cell transplantation (pTCD-PSCT) has the potential advantages of providing a high stem cell dosedoi:10.1016/j.bbmt.2018.10.003 pmid:30312755 pmcid:PMC7122955 fatcat:p6vk3xqiuzdzbd3jhsjdqwf57a
more »... to facilitate rapid engraftment, maintaining cells that may facilitate engraftment, and decreasing GVHD risk compared with T cell-replete HSCT. Here, we report a single-institution, retrospective experience of unrelated donor pTCD-PSCT for pediatric patients with NMHD. From 2014 to 2017, 12 pediatric patients with transfusion-dependent NMHD underwent matched unrelated donor (MUD) or mismatched unrelated donor (MMUD) pTCD HSCT in our center using disease-specific conditioning. Donor PSCs underwent CD3+ T cell and CD19+ B cell depletion using CliniMACS, followed by a targeted addback of 1 × 105 CD3+ T cells/kg to the graft before infusion. All 12 patients demonstrated rapid trilinear engraftment. At a median follow-up of 740days (range, 279 to 1466), all patients were alive with over 92% total peripheral blood donor chimerism and without transfusion dependence or recurrence of their underlying hematologic disease. Immune reconstitution was rapid and comparable with T cell-replete HSCT. No patients developed severe acute GVHD (grades III to IV) or chronic extensive GVHD, and all patients had discontinued systemic immune suppression. Viral reactivations were common, but no patient developed symptoms of life-threatening infectious disease. Our data indicate that MUD and MMUD pTCD-PSCTs are safe and effective approaches that enable rapid engraftment and immune reconstitution, prevent severe GVHD, and expand availability of HSCT to any patients with NMHD who have closely MUDs.
mono-resistant TB. ... Treatment was successful in 74.0% of INH mono-resistant and 77.4% of susceptible TB cases. ... with INH mono-resistance. ...doi:10.25646/6161 fatcat:xjcb5qif7fhn5plfa535n7lhgu
Theodossopoulos Dimitrios, “The ‘Turks’ in the imagination of the ‘Greeks’”, introduction to Dimitrios Theodossopoulos (ed.), When Greeks Think about Turks: The View from Anthropology, theme issue of ... Akturk Sener, “Persistence of the Islamic Millet as an Ottoman Legacy: Mono-Religious and Anti-Ethnic Definition of Turkish Nationhood”, Middle Eastern Studies , vol. 45, no. 6, 2009, p. 893-909. ...doi:10.4000/eac.2455 fatcat:34iakcr6cjbgpppqipywowbaw4
Dimitrios Ioannis Patoulias, Maria-Styliani Kalogirou Finally, we would like to comment on the impact of direct-acting antiviral (DAA) therapy. ... patients; it has been shown in a recent meta-analysis that patients with HCV/HIV co-infection have a significant increase in the risk of cardiovascular disease by 24%, compared to those patients with HIV mono-infection ...doi:10.5114/ceh.2020.93060 pmid:32166127 pmcid:PMC7062118 fatcat:slycyqznavf7tkx7ljttrkudii
Prio Mono- graphs from the International Peace Research Institute, Oslo, 1. ... With photographs by Dimitrios Harissiadis. New York: Random House, 1968. 303 pp., bibliog- raphy, illustrations, index, 45 color plates (1 frontispiece). $17.95. Heizer, Robert F., ed. and annotator. ...
and -DAS28: 3.2-5.1 and -1 st bDMARD or 1 st JAK inhibitor to: Continuation of csDMARD ± bDMARD -2 nd bDMARD or -2 nd JAK-inhibitor ± csDMARD -1 st bDMARD or -1 st JAK-bDMARD or JAK inhibitor ± csDMARD Mono-or ...doi:10.31138/mjr.31.1.163 pmid:32676575 pmcid:PMC7361183 fatcat:ktl3y7u4mffk5bakvqosoovsmi
In conclusion, we observed increased all-cause mortality with initial azole mono- therapy compared to that with early treatment with an echinocandin or polyene in cancer patients with C. glabrata fungemia ...doi:10.1128/aac.00631-17 pmid:28584138 pmcid:PMC5527607 fatcat:kxanirbk7ze6tfej3pcjjvxk5m
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