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Differential Cost Analysis with Simultaneous Potentials and Anti-potentials [article]

Đorđe Žikelić, Bor-Yuh Evan Chang, Pauline Bolignano, Franco Raimondi
2022 arXiv   pre-print
evaluates to an upper bound for the cost incurred in the first program and an anti-potential function that evaluates to a lower bound for the cost incurred in the second.  ...  In particular, our method computes a threshold value for the maximal difference in cost between two program versions simultaneously using two kinds of cost-bound summaries -- a potential function that  ...  Acknowledgements We thank Shaun Willows, Thomas Lugnet, and the Living Room Application Vending team for suggesting threshold bounds as a developer-friendly way to interact with a differential cost analyzer  ... 
arXiv:2204.00870v2 fatcat:obzl7tdamreovnqajfaqkqzmf4

A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells

Soichiro Ogaki, Mayu Morooka, Kaito Otera, Shoen Kume
2015 Scientific Reports  
We established a rapid and cost effective system for differentiation of human induced pluripotent stem (iPS) cells into definitive endoderm (DE) cells.  ...  Data were recorded using the BD FACS Diva Software program (BD Pharmingen) and analysed using the FlowJo program (Tree Star). Cell cycle analysis. Cells were dissociated with 0.25% trypsin.  ...  (B,C) Flow cytometry analysis. Graded concentrations of DMSO were tested for DE differentiation, and 0.8% DMSO had the strongest effect for potentiating differentiation into CD117+ CXCR4+ cells (B).  ... 
doi:10.1038/srep17297 pmid:26616277 pmcid:PMC4663490 fatcat:pv4p6oevizgcpibojx7odqwv7a

Highly Specific Electrochemical Analysis of Cancer Cells using Multi-Nanoparticle Labeling

Ying Wan, Yi-Ge Zhou, Mahla Poudineh, Tina Saberi Safaei, Reza M. Mohamadi, Edward H. Sargent, Shana O. Kelley
2014 Angewandte Chemie International Edition  
For this reason, their specific and sensitive detection is of intense interest. Herein, we report a new, chipbased strategy for the automated analysis of cancer cells.  ...  The electrochemical assay allows simultaneous detection of multiple different biomarkers on the surfaces of cancer cells, enabling discrimination between cancer cells and normal blood cells.  ...  The electrochemical readout of several markers simultaneously requires redox-active probes with well-separated potentials.  ... 
doi:10.1002/anie.201407982 pmid:25283158 fatcat:tcenmf52bbdf5k3megzds6pzue

Highly Specific Electrochemical Analysis of Cancer Cells using Multi-Nanoparticle Labeling

Ying Wan, Yi-Ge Zhou, Mahla Poudineh, Tina Saberi Safaei, Reza M. Mohamadi, Edward H. Sargent, Shana O. Kelley
2014 Angewandte Chemie  
For this reason, their specific and sensitive detection is of intense interest. Herein, we report a new, chipbased strategy for the automated analysis of cancer cells.  ...  The electrochemical assay allows simultaneous detection of multiple different biomarkers on the surfaces of cancer cells, enabling discrimination between cancer cells and normal blood cells.  ...  The electrochemical readout of several markers simultaneously requires redox-active probes with well-separated potentials.  ... 
doi:10.1002/ange.201407982 fatcat:mlpnknz7bjhmjb6axrjyp2fcre

Label-free, scalable and point-of-care imaging platform for rapid analysis of biomarker

Luc Dümpelmann, Roland A. Terborg, Josselin Pello, Ilaria Mannelli, Filiz Yesilköy, Alexander Belushkin, Yasaman Jahani, Nuria Fabri-Faja, Priyanka Dey, Olalla Calvo, Maria Carmen Estévez Alberola, Anna Fàbrega (+7 others)
2019 Advances in Microscopic Imaging II  
It enables simultaneous analysis of potentially up to 10,000 functionalized microarray spots with different biomarkers with fast time-to-results (few minutes) and by consuming a small sample volume (~10  ...  The proposed technology incorporates a portable and low-cost lens-free imaging reader made of consumer electronic components, plasmonic microarrays with distinct functionalization, and user-friendly software  ...  The lens-free configuration (large field-ofview) allows simultaneous label-free detection and analysis of potentially more than 10,000 biomarkers (highly scalable) within minutes.  ... 
doi:10.1117/12.2525878 fatcat:j6dgncf4vfaqph62cciw5izura

Generation of hypoimmunogenic induced pluripotent stem cells by CRISPR-Cas9 system and detailed evaluation for clinical application

Yuko Kitano, Sayaka Nishimura, Tomoaki M. Kato, Anna Ueda, Kaho Takigawa, Masafumi Umekage, Masaki Nomura, Ayane Kawakami, Haruna Ogawa, Huaigeng Xu, Akitsu Hotta, Naoko Takasu (+1 others)
2022 Molecular Therapy: Methods & Clinical Development  
RNA-seq and flow cytometry analyses confirmed the successful depletion of HLAs, and lineage-specific differentiation into cardiomyocytes was verified.  ...  Here, we optimized the protocol for good manufacturing practice (GMP)-compatible CRISPR-Cas9 genome editing to deplete the three gene locus (HLA-A, HLA-B, and CIITA genes) simultaneously in HLA homozygous  ...  Hirofumi Harashima (AS ONE) for providing technical assistance with the genomic analysis. We thank Dr. Shin Kaneko (CiRA, Kyoto University) and Dr.  ... 
doi:10.1016/j.omtm.2022.05.010 pmid:35755947 pmcid:PMC9198376 fatcat:q7xcdqxbenclff3mxo3dkmf25a

Sex differences in lizard escape decisions vary with latitude, but not sexual dimorphism

D. S. M. Samia, A. P. Moller, D. T. Blumstein, T. Stankowich, W. E. Cooper
2015 Proceedings of the Royal Society of London. Biological Sciences  
This novel latitudinal effect on sex-specific anti-predator behaviour has important implications for responses to climate change and for the relative roles of natural and sexual selection in different  ...  We conducted a meta-analysis of 28 studies (42 species) of sex differences in risk-taking behaviour in lizards and tested whether these differences could be explained by sexual dichromatism, by sexual  ...  about phylogenetic meta-analysis.  ... 
doi:10.1098/rspb.2015.0050 pmid:25788595 pmcid:PMC4389619 fatcat:ckvmpxrcabeulcaur7ycvabi7m

Raman spectroscopy and coherent anti-Stokes Raman scattering imaging: prospective tools for monitoring skeletal cells and skeletal regeneration

Catarina Costa Moura, Rahul S. Tare, Richard O. C. Oreffo, Sumeet Mahajan
2016 Journal of the Royal Society Interface  
The ability for controlled modification of SSCs could provide significant therapeutic potential in regenerative medicine, with the prospect to permanently repopulate a host with stem cells and their progeny  ...  Here we review the applications of Raman spectroscopy and CARS imaging in stem-cell research, and discuss the potential of these two techniques for evaluating SSCs, skeletal tissues and skeletal regeneration  ...  , with widespread potential in other hard and soft tissues.  ... 
doi:10.1098/rsif.2016.0182 pmid:27170652 pmcid:PMC4892269 fatcat:27rtlmqb5fbc3pdlfzpqohj2je

High-Density Microwell Chip for Culture and Analysis of Stem Cells

Sara Lindström, Malin Eriksson, Tandis Vazin, Julia Sandberg, Joakim Lundeberg, Jonas Frisén, Helene Andersson-Svahn, Joseph Najbauer
2009 PLoS ONE  
This microwell chip has high potential for a wide range of applications, for example directed differentiation assays and screening of reprogramming factors, opening up considerable opportunities in the  ...  With recent findings on the role of reprogramming factors on stem cells, in vitro screening assays for studying (de)differentiation is of great interest.  ...  Acknowledgments We are grateful to Prof Outi Hovatta and Ms Susanne Stro ¨m (Karolinska Institute, Sweden) for kindly providing us with human ES cells for initial tests.  ... 
doi:10.1371/journal.pone.0006997 pmid:19750008 pmcid:PMC2736590 fatcat:kmbrqm7wwnad7ezwx4eubfhjfi

GSK-3 Inhibition as a Therapeutic Approach Against SARs CoV2: Dual Benefit of Inhibiting Viral Replication While Potentiating the Immune Response

Christopher E. Rudd
2020 Frontiers in Immunology  
To my knowledge, no other drugs exist with the potential to simultaneously target both viral replication and immune response against SARs CoV2.  ...  My hypothesis is that the repurposing of low-cost inhibitors of GSK-3 such as lithium will limit SARS-CoV2 infections by both reducing viral replication and potentiating the immune response against the  ...  To my knowledge, no other drugs exist with the potential to simultaneously target both viral replication and immune response against SARs CoV2.  ... 
doi:10.3389/fimmu.2020.01638 pmid:32695123 pmcid:PMC7333796 fatcat:mr2yfxx2xvgurhqt65nvlqxr6u

Microchip-based ultrafast serodiagnostic assay for tuberculosis

Vigneshwaran Mani, Bhairav Paleja, Karima Larbi, Pavanish Kumar, Jo Ann Tay, Jie Yee Siew, Fatih Inci, ShuQi Wang, Cynthia Chee, Yee Tang Wang, Utkan Demirci, Gennaro De Libero (+1 others)
2016 Scientific Reports  
Receiver operating characteristic analysis indicated good accuracy for differentiating ATB from HC (AUC = 0.77).  ...  Here, we report a simple and low-cost microchip-based TB ELISA (MTBE) platform for the detection of anti-mycobacterial IgG in plasma samples in less than 15 minutes.  ...  Acknowledgements This work was supported by A*STAR-CIMIT grant and BMRC-SERC diagnostic grant.  ... 
doi:10.1038/srep35845 pmid:27775039 pmcid:PMC5075771 fatcat:sxwm72s6gfftlku3gzqjb7pbre

Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

Xuan Liu, Hui Jiang
2017 Sensors  
led to great achievements in fast quantitative and simultaneous testing with extremely high sensitivity and specificity.  ...  Biosensors for antibodies are summarized, focusing on potential applications in clinical laboratory diagnosis, such as a series of biomarkers for infectious diseases and autoimmune diseases, and an evaluation  ...  the Nanjing Medical Science and Technique Development Foundation.  ... 
doi:10.3390/s17122805 pmid:29207528 pmcid:PMC5750678 fatcat:6zgba3ty4zdvzlfk7yjahibvl4

Commercial Screen-Printed Electrodes Based on Carbon Nanomaterials for a Fast and Cost-Effective Voltammetric Determination of Paracetamol, Ibuprofen and Caffeine in Water Samples

Núria Serrano, Òscar Castilla, Cristina Ariño, M. Diaz-Cruz, José Díaz-Cruz
2019 Sensors  
) in water by differential pulse voltammetry (DPV).  ...  electrode (SPGPHE) were in a pioneer way tested as sensors for the simultaneous determination of the two most consumed pain-killers, paracetamol (PA) and ibuprofen (IB), and the stimulant caffeine (CF  ...  Results and Discussion Optimization of Condition Media and the Potential Range Both potential range and condition media were optimized in connection with the simultaneous determination of PA, IB, and  ... 
doi:10.3390/s19184039 pmid:31546788 pmcid:PMC6767221 fatcat:rtenv3l3rnfg3a7mpdpew6dl5q

Multiplexed Electrochemical Immunosensors for Clinical Biomarkers

2017 Sensors  
with multiplexed determination, microfabrication technology and decentralized determinations, features which made them very attractive for integration in point-of-care (POC) devices.  ...  of several clinical biomarkers by using the same analytical device hold enormous potential for early detection and personalized therapy and will simplify the diagnosis providing more information in less  ...  Acknowledgments: Financial support of the CTQ2015-70023-R and CTQ2015-64402-C2-1-R (Spanish Ministerio de Economía y Competitividad Research Projects) and S2013/MT3029 (NANOAVANSENS Program from the Comunidad  ... 
doi:10.3390/s17050965 pmid:28448466 pmcid:PMC5464191 fatcat:rmuf63f3h5btvkocdzhnsubeni

A disposable two-throughput electrochemical immunosensor chip for simultaneous multianalyte determination of tumor markers

Jie Wu, Zhijie Zhang, Zhifeng Fu, Huangxian Ju
2007 Biosensors & bioelectronics  
Under optimal conditions the immunosensor array could be used for fast simultaneous electrochemical detection of CA 19-9 and CA 125 with the limits of detection of 0.2 and 0.4 U/ml, respectively.  ...  The proposed method for preparation of immunosensor array could be conveniently used for fabrication of disposable electrochemical biochip with high throughput and possessed the potential of mass production  ...  Natural Science Foundation of China and the Science Foundation of Jiangsu (BS2006006, BS2006074) for financial support of this research and the Nanjing TianDing Institute of Biotechnology for screen-printing  ... 
doi:10.1016/j.bios.2007.03.023 pmid:17475473 fatcat:bpvj5caihrhmvih7uqtrjxlodm
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