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Detection of Potato Storage Disease via Gas Analysis: A Pilot Study Using Field Asymmetric Ion Mobility Spectrometry

Massimo Rutolo, James Covington, John Clarkson, Daciana Iliescu
2014 Sensors  
of infection (for early detection).  ...  Here, we explore the possibility of early detection of the disease via gas/vapor analysis, in a laboratory environment, using a recent technology known as FAIMS (Field Asymmetric Ion Mobility Spectrometry  ...  FAIMS Technology The purpose of an Ion Mobility Spectrometer is to separate complex chemical mixtures into individual or groups of chemicals based on their mobility in high electric fields.  ... 
doi:10.3390/s140915939 pmid:25171118 pmcid:PMC4208154 fatcat:akrpfw2d3fcg7liogghfsq7cce

The Detection of Wound Infection by Ion Mobility Chemical Analysis

Emma Daulton, Alfian Wicaksono, Janak Bechar, James A. Covington, Joseph Hardwicke
2020 Biosensors  
VOC detection using GC-IMS has the potential to serve as a diagnostic tool for the differentiation of infected and non-infected wounds and facilitate the treatment of wound infections that is cost effective  ...  This study describes the use of a device to differentiate VOCs produced by an infected wound vs. colonised wound.  ...  Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/bios10030019 pmid:32121452 pmcid:PMC7146168 fatcat:mvccy5rv65hz5nq72e7lcau3sa

Sniffing Out Urinary Tract Infection—Diagnosis Based on Volatile Organic Compounds and Smell Profile

Valentin-Mihai Dospinescu, Akira Tiele, James A. Covington
2020 Biosensors  
Current available methods for the clinical diagnosis of urinary tract infection (UTI) rely on a urine dipstick test or culturing of pathogens.  ...  ), field asymmetric ion mobility spectrometry (FAIMS) and electronic noses (eNoses) used for the diagnosis of UTI.  ...  Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/bios10080083 pmid:32717983 pmcid:PMC7460005 fatcat:4fp7snk3kvfvlmden6uhpdyosq

Applications of Electronic-Nose Technologies for Noninvasive Early Detection of Plant, Animal and Human Diseases

Alphus Dan Wilson
2018 Chemosensors  
The development of electronic-nose (e-nose) technologies for disease diagnostics was initiated in the biomedical field for detection of biotic (microbial) causes of human diseases during the mid-1980s.  ...  Progress in the discovery of new e-nose technologies developed for biomedical applications has exploded with new information and methods for diagnostic sampling and disease detection, identification of  ...  Conflicts of Interest: The author declares no conflict of interest.  ... 
doi:10.3390/chemosensors6040045 fatcat:lbn6gmimrzgchlsy6bdgrg6uoa

Emerging Methods of Monitoring Volatile Organic Compounds for Detection of Plant Pests and Disease

Samantha MacDougall, Fatih Bayansal, Ali Ahmadi
2022 Biosensors  
This paper focuses on applications of different gas-sensing technologies from over the past decade to detect plant pests and diseases more efficiently.  ...  Furthermore, most of these methods lack research on their use under field conditions.  ...  Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/bios12040239 pmid:35448299 pmcid:PMC9025064 fatcat:lmv5qlx2t5g7bdiozlxxmirzea

Diagnosing gastrointestinal illnesses using fecal headspace volatile organic compounds

Daniel K Chan
2016 World Journal of Gastroenterology  
Electronic nose devices are analytical and pattern recognition platforms that can utilize mass spectrometry or electrochemical sensors to detect these VOCs in gas samples.  ...  of stool.  ...  Based on responses, the staff were able to discern C. difficile-infected stool with 55% sensitivity and 83% specificity, with a negative predictive value of 92% [12] .  ... 
doi:10.3748/wjg.v22.i4.1639 pmid:26819529 pmcid:PMC4721995 fatcat:a6w477j6nbgh7nzlaqfqu6raem

Expanding the knowledge on dried blood spots and LC-MS-based protein analysis: two different sampling materials and six protein targets

Cecilie Rosting, Astrid Gjelstad, Trine Grønhaug Halvorsen
2017 Analytical and Bioanalytical Chemistry  
FAIMS doubled the total number of detected proteins compared to sample run without FAIMS and was also shown to work complementary to LC-MS analysis without FAIMS.  ...  The top-down approach is based on intact analysis of the proteins and will reveal the whole mass of the molecule, which is typically useful in detection of posttranslational modifications (PTMs).  ... 
doi:10.1007/s00216-017-0280-3 pmid:28299418 fatcat:epabjmvwfvdxlnhddkycpkfn3y

Transcriptome analysis of sheep oral mucosa response to Orf virus infection

Huaijie Jia, Leilei Zhan, Xiaoxia Wang, Xiaobing He, Guohua Chen, Yu Zhang, Yuan Feng, Yaxun Wei, Yi Zhang, Zhizhong Jing, Pierre Roques
2017 PLoS ONE  
samples (T0, T3, T7 and T15) were collected on day 0, 3, 7 and 15 after ORFV infection respectively.  ...  Gene Ontology (GO) analyses of the DEGs from these comparisons, revealed that ORFV might provoke vigorous immune response of the host cells during the early stage of infection.  ...  Acknowledgments We would like to thank the members of ABLife Inc for their helpful discussions and critical reading of the manuscript.  ... 
doi:10.1371/journal.pone.0186681 pmid:29073164 pmcid:PMC5658058 fatcat:qhlaplscobbhpcylfd6bxf3nqy

Use of GC-IMS for detection of volatile organic compounds to identify mixed bacterial culture medium

Yanyi Lu, Lin Zeng, Min Li, Bowen Yan, Dandan Gao, Bangfu Zhou, Weiping Lu, Qinghua He
2022
In order to explore the possibility to identify common wound infection bacteria in mixed culture with gas chromatograph-ion migration spectroscopy (GC-IMS), the headspace gas of single and mixed cultures  ...  of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were detected and analyzed by GC-IMS system.  ...  As far as we know, this is the first study on the possibility to identify common wound-infecting bacteria in mixed culture using GC-IMS.  ... 
doi:10.1186/s13568-022-01367-0 pmid:35244795 pmcid:PMC8897540 fatcat:cujb5dxq5vfhnomuuookgik2cm

Conference 2014: Promoting Today's Ideas for Tomorrow's Medicine: Pharmaceutical Science to Regulatory Science. An international symposium held jointly by CSPS and CC-CRS, June 10-13, 2014, Montreal, QC, Canada

Canadian Society for Pharmaceutical Sciences (CSPS)
2014 Journal of Pharmacy & Pharmaceutical Sciences  
/CSPS Early Career Award - "Development of Intravaginal Microbicides for the Prevention of HIV Infection"Building Opportunity Day:- The Life Sciences Industry in Canada: Now and the Future...- Start-up  ...  of Toronto: CSPS Lifetime Achievement Award - "Sense and Sensibility (?)  ...  Technology with a PhD in Organic Chemistry in 1997, Gerald joined the Cambridge-based biotech startup company NeoGenesis as one of its first scientists.  ... 
doi:10.18433/j30g62 fatcat:p4tsrhlgorgqddypi6pihsmomq

YKL-40—A Protein in the Field of Translational Medicine: A Role as a Biomarker in Cancer Patients?

Nicolai A. Schultz, Julia S. Johansen
2010 Cancers  
Plasma YKL-40 is also elevated in patients with other diseases than cancer, e.g., severe infections, cardiovascular disease, diabetes, chronic obstructive lung disease, asthma, liver fibrosis and rheumatoid  ...  However, there is not yet sufficient data to support determination of plasma YKL-40 outside research projects as a biomarker for screening of gastrointestinal cancer and determination of treatment response  ...  Acknowledgements The authors thank Kjeld Møllgård, University of Copenhagen for preparing Figure 1 .  ... 
doi:10.3390/cancers2031453 pmid:24281168 pmcid:PMC3837317 fatcat:oobdt2mxgfgctcfezuatdik6hq

2018 Advances in Animal Biosciences

2018 Advances in Animal Biosciences  
The aim of the study was to evaluate effects of BW on N-digestion and N-utilization in growing pigs.  ...  This study is part of the Feed-a-Gene Project, EU H2020 Program no 633531. 488 Effects of oral monosodium glutamate administration on serum metabolomics of suckling piglets 522 Foetal development  ...  As such, these neurochemicals become a common evolutionary language by which the bacteria communicate with the host and vice versa. 131 The effect of environmental challenge on gut microbiota composition  ... 
doi:10.1017/s2040470018000225 fatcat:3ujwds5xyjfclpnnho2nkfhoum

Exploring ambient mass spectrometry capacities for rapid detection and phenotyping of pseudomonas aeruginosa infection in cystic fibrosis patients

Emmanuelle Bardin, Zoltan Takats, Jane Davies, Andrew Bush, Eric Alton, UK Cystic Fibrosis Trust
2021
This thesis explores the potential of novel ambient mass spectrometric (MS) tools for the phenotyping of P. aeruginosa and its detection in non-invasive samples.  ...  REIMS could not detect P. aeruginosa in sputum and urine pellets, nor could DESI-MS on skin secretions. Nevertheless, the rich sputum metabolic profile and skin lipidome contained po [...]  ...  The Lonestar® developed by the Owlstone Company (Cambridge, UK) is based on field asymmetric ion mobility spectrometry (FAIMS) technology.  ... 
doi:10.25560/85487 fatcat:2abvhaphcjfvplh2dxdr5p3rly

CRACking the Riddle [article]

Roland Schwarzer, Humboldt-Universität Zu Berlin, Humboldt-Universität Zu Berlin
2017
It was presumed, that in particular viruses make use of plasma membrane lipid rafts to augment the infection process and spread efficiently.  ...  In the present study, fluorescence lifetime imaging microscopy was used to study lipid raft partitioning of the HIV-1 glycoprotein gp41 by detecting Foerster Resonance Energy Transfer between fluorescently  ...  However, the most common preparation method based on PFA/DTT is supposed to produce artificial results and not to be reflective of native conditions since DTT is a strong depalmitoylation agent 97  ... 
doi:10.18452/17010 fatcat:gz5kvdhg75c3posmyrm67khz5m

The immunomodulatory and cytotoxic effects of different forms of recombinant adenylate cyclase toxin

Yiu Chong Gordon Cheung
2006
g protein/ml) and this paralleled low levels of caspase 3/7 activity, a measure of apoptotic activity. At higher toxin concentrations of CyaA (> 0.5 ?  ...  Thus, inhibition of the zymosan-stimulated oxidative burst by CyaA was a result of both invasive and AC enzymic activities.  ...  Different volumes of bacteria gave different bacteria: leukocyte ratios or different multiplicities of infection (MOI). As a control, 30 pi of unopsonised bacteria were incubated with 120 pi of HBSA.  ... 
doi:10.5525/gla.thesis.71028 fatcat:gbwjjwliqzaupfacjjdprbofwu
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