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Detection of Electrical Activity of Pancreatic Beta-cells Using Micro-electrode Arrays

Yannick Bornat, Matthieu Raoux, Youssef Boutaib, Fabrice Morin, Gilles Charpentier, Jochen Lang, Sylvie Renaud
2010 2010 Fifth IEEE International Symposium on Electronic Design, Test & Applications  
In this position paper we propose a new approach to provide online in-vivo recordings of organ activity in real time and to overcome three major shortcuts of currently used invasive glucose sensors.  ...  Consequently, using the -cell as a signal integrator combined with ASIC technology ensures a physiological signal and its read-out in real time thus avoiding complex algorithms.  ...  Closed-loop system architecture We propose to record the electrical activity of beta(ß)cells using multiple and non-invasive extracellular electrodes by the means of micro-electrode arrays (MEAs) [19]  ... 
doi:10.1109/delta.2010.60 dblp:conf/delta/BornatRBMCLR10 fatcat:3l4fjormfbafve3tjxqlpzfd6e

Non-invasive long-term and real-time analysis of endocrine cells on micro-electrode arrays

Matthieu Raoux, Yannick Bornat, Adam Quotb, Bogdan Catargi, Sylvie Renaud, Jochen Lang
2012 Journal of Physiology  
Non-invasive long-term and real-time analysis of endocrine cells on micro-electrode arrays.  ...  Here we report a novel approach to obtain simultaneous long-term electrical recordings of different islet cell types using multi-electrode arrays.  ...  Figure 1A gives the general outline of our approach. Clonal β-cells or primary islet cells are cultured on micro-electrode arrays (MEA) that contain multiple plates to record electrical signals.  ... 
doi:10.1113/jphysiol.2011.220038 pmid:22199167 fatcat:h3usqst7pfdgxlohvrhmtir6x4

A novel bioelectronic glucose sensor to process distinct electrical activities of pancreatic beta-cells

Quang Vinh Nguyen, Anton Caro, Matthieu Raoux, Adam Quotb, Jean-Baptiste Floderer, Yannick Bornat, Sylvie Renaud, Jochen Lang
2013 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)  
A novel bioelectronic glucose sensor to process distinct electrical activities of pancreatic beta-  ...  to process distinct electrical activities of pancreatic beta-cells * body for insulin [5] .  ...  Fig. 1 : 1 Extracellular recording of electrical signals from mouse islet cells cultured on multi-electrode arrays (MEA).  ... 
doi:10.1109/embc.2013.6609465 pmid:24109652 dblp:conf/embc/NguyenCRQFBRL13 fatcat:gopv7u7mdre2fd5rurhrks5ryu

Guiding pancreatic beta cells to target electrodes in a whole-cell biosensor for diabetes

Eileen Pedraza, Aleksandar Karajić, Matthieu Raoux, Romain Perrier, Antoine Pirog, Fanny Lebreton, Stéphane Arbault, Julien Gaitan, Sylvie Renaud, Alexander Kuhn, Jochen Lang
2015 Lab on a Chip  
We present a method for delivering cells to target electrodes of a whole-cell bioelectronic sensor for multi-parametric sensing of glucose and physiological hormone regulators.  ...  " We would like to acknowledge the help of the Animal Facility of Bordeaux University.  ...  We are very grateful to Valerie Ravaine for assistance with the zeta-potential measurements and we would also like to thank Bertrand Goudeau for granting us access to and assisting us with the Leica stereoscopic  ... 
doi:10.1039/c5lc00616c pmid:26282013 fatcat:ikqsj4e43zdohm6jwxjpdwibba

Slow potentials encode intercellular coupling and insulin demand in pancreatic beta cells

Fanny Lebreton, Antoine Pirog, Isma Belouah, Domenico Bosco, Thierry Berney, Paolo Meda, Yannick Bornat, Bogdan Catargi, Sylvie Renaud, Matthieu Raoux, Jochen Lang
2015 Diabetologia  
Pharmacological tools and the use of beta cell specific knockout mice showed that SPs reflected cell coupling via connexin 36.  ...  Extracellular electrical activities received on each electrode were continuously amplified and recorded for offline characterisation.  ...  Pedraza (University of Bordeaux, Bordeaux, France) for critical reading of the manuscript. We gratefully acknowledge the help of the Animal Facility of the University of Bordeaux.  ... 
doi:10.1007/s00125-015-3558-z pmid:25788295 fatcat:mhgc2a2mh5c2rhe67lxohx7o6m

Latest Trends in Biosensing for Microphysiological Organs-on-a-Chip and Body-on-a-Chip Systems

Kratz, Höll, Schuller, Ertl, Rothbauer
2019 Biosensors  
An important aspect of any diagnostic device and cell analysis platform, however, is the integration and application of a variety of sensing strategies to provide reliable, high-content information on  ...  and patient-derived induced pluripotent stem cells (iPSCs) into microfluidic devices.  ...  measurements of oxygen sensitive platinum-based dye (PtTPTBPF) [29] Pancreatic islets Glucose concentration- dependent micro-organ activity B-cells of pancreatic islets adult male C57BL  ... 
doi:10.3390/bios9030110 pmid:31546916 pmcid:PMC6784383 fatcat:sodyjgo6lbekvgybceybi734pq

Rapid Detection of Protein Aggregation and Inhibition by Dual Functions of Gold Nanoplasmonic Particles: Catalytic Activator and Optical Reporter

Inhee Choi, Elizabeth Lee, Minsun Song, Luke P. Lee
2014 Biophysical Journal  
A fast, sensitive method to detect the release of acetylcholine at the surface of a single cell is needed to gather data about the kinetics of exocytosis events in these systems.  ...  enzyme activity.  ...  Type 1 diabetes (T1D) is characterized as a chronic autoimmune disease caused by a selective inflammatory destruction of the insulin producing beta cells in the pancreatic islets of Langerhans.  ... 
doi:10.1016/j.bpj.2013.11.2344 fatcat:kj7qjiselvcyheuruf4iirwqpe

The Current Trends of Biosensors in Tissue Engineering

Yi-Chen Ethan Li, I-Chi Lee
2020 Biosensors  
Cell polarity and cell behaviors such as proliferation, differentiation, stimulation response, and metabolism detection are included.  ...  Compared to standard enzyme-linked immunosorbent assay (ELISA) analytical technology, biosensors provide a strategy to real-time and on-site monitor micro biophysiological signals via a combination of  ...  Furthermore, ultra-flexible and micro-electrocorticography arrays fabricated by glassy carbon electrodes have also been used to stimulate and record brain activity with low background noise [89] .  ... 
doi:10.3390/bios10080088 pmid:32756393 fatcat:3fgpln37tvdchemy756vtdogaa

Cell Assays in Microfluidics [chapter]

Irena Barbulovic-Nad
2015 Encyclopedia of Microfluidics and Nanofluidics  
However, fabrication of electrodes is a technical challenge and the electrical fields used in detection can interfere with onchip processes such as electrophoresis.  ...  [24] seeded neurons in an array of chambers in a microfluidic network integrated with an array of electrodes (Fig. 4b) .  ... 
doi:10.1007/978-1-4614-5491-5_183 fatcat:gu5kanwcenelvkfcdf7lv45rbi

Cell Assays in Microfluidics [chapter]

Irena Barbulovic-Nad
2014 Encyclopedia of Microfluidics and Nanofluidics  
However, fabrication of electrodes is a technical challenge and the electrical fields used in detection can interfere with onchip processes such as electrophoresis.  ...  [24] seeded neurons in an array of chambers in a microfluidic network integrated with an array of electrodes (Fig. 4b) .  ... 
doi:10.1007/978-3-642-27758-0_183-2 fatcat:kud7bl3xafg2boqp6oyzzjsece

Solving Medical Problems with BioMEMS

E. Seker, Jong Hwan Sung, M. L. Shuler, M. L. Yarmush
2011 IEEE Pulse  
For example, when a cell is exposed to a certain drug, it may respond by changing its morphology or by secreting specific molecules that can be detected by specific assays.  ...  During the exercise, the student exposes the circuit to a range of electrical signals and captures the voltage and current characteristics.  ...  Electrical Stimulation Some cells, such as neurons and endocrine cells (e.g., pancreatic beta cells), respond to an electrical stimulation by neural transmission of signals and secretion of hormones (e.g  ... 
doi:10.1109/mpul.2011.942928 pmid:22147069 pmcid:PMC4490689 fatcat:tbmet4qtlrf7zg4ycws6i4nrkm

Multi-Electrode Array Analysis Identifies Complex Dopamine Responses and Glucose Sensing Properties of Substantia Nigra Neurons in Mouse Brain Slices

Nadja Mannal, Katharina Kleiner, Michael Fauler, Antonios Dougalis, Christina Poetschke, Birgit Liss
2021 Frontiers in Synaptic Neuroscience  
We studied this activity and its control by dopamine, NCS-1, and glucose with extracellular multi-electrode array (MEA) recordings from midbrain slices of juvenile and adult mice.  ...  To directly address and quantify glucose-sensing properties of SN DA neurons, we continuously monitored their electrical activity, while altering extracellular glucose concentrations stepwise from 0.5  ...  In the latter study, micro-graphitic single crystal diamond Multi Electrode Arrays (µG-SCD-MEAs) were used, that allow, in addition to recording of electrical activity, amperometric analysis of dopamine  ... 
doi:10.3389/fnsyn.2021.635050 pmid:33716704 pmcid:PMC7952765 fatcat:7dd5jfyjabfubnbeuqmjqunr4q

Arachidonic Acid/ppara Enhancement of Ca2+-Regulated Exocytosis in Antral Mucous Cells of Guinea Pig

Takashi Nakahari
2010 Biophysical Journal  
Here we report the activation of an intercellular signaling pathway in sea urchin embryos by membrane wounding that evokes calcium spikes in neighboring cells.  ...  Co-injection of mRNA encoding human dysferlin with the inhibitory morpholino rescued signaling activity.  ...  Here we describe our efforts to use the same electrode to electrically stimulate the adjacent cell and subsequently measure exocytosis using amperometry.  ... 
doi:10.1016/j.bpj.2009.12.3738 fatcat:52kg2lklefgbtauhvsgihqalsq

Application of Nano-biotechnology for Improvement in Therapeutic Approaches for the Treatment of Diabetes

Mukhopadhyay A, Prosenjit M
2018 Journal of Clinical and Molecular Endocrinology  
In Type 1 diabetes enough insulin is not produced by pancreatic beta cells, whereas in Type 2 the body's cells poorly respond to the presence of insulin.  ...  level, and development of smart imaging molecule to visualise and quantify beta cells etc.  ...  Acknowledgements The authors are thankful to Director, Indian Institute of Technology, Mandi for his encouragement and financial support.  ... 
doi:10.21767/2572-5432.100047 fatcat:r45nunsrqraf7kljt4ikeotkxy

Multimed: An Integrated, Multi-Application Platform for the Real-Time Recording and Sub-Millisecond Processing of Biosignals

Antoine Pirog, Yannick Bornat, Romain Perrier, Matthieu Raoux, Manon Jaffredo, Adam Quotb, Jochen Lang, Noëlle Lewis, Sylvie Renaud
2018 Sensors  
Embedded onto a Field Programmable Gate Array (FPGA), these modules utilize full-hardware signal processing to lower processing latency.  ...  As more and more excitable cell mechanics are being investigated and exploited, the need for flexible electrophysiology setups becomes apparent.  ...  Conflicts of Interest: The authors declare no conflict of interest. Data Availability Statement: Please contact author for data requests.  ... 
doi:10.3390/s18072099 pmid:29966339 pmcid:PMC6069272 fatcat:ukdul5rc6rduxcgbwaw6wbmebi
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