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Structure of Isolated Nucleocapsids from Venezuelan Equine Encephalitis Virus and Implications for Assembly and Disassembly of Enveloped Virus

A. Paredes, K. Alwell-Warda, S. C. Weaver, W. Chiu, S. J. Watowich
2003 Journal of Virology  
three-dimensional structure of a nucleocapsid isolated from a single-stranded enveloped RNA virus.  ...  Venezuelan equine encephalitis virus (VEEV) is an important human and equine pathogen in the Americas, with widespread reoccurring epidemics extending from South America to the southern United States.  ...  .), and the Sealy Center for Structural Biology.  ... 
doi:10.1128/jvi.77.1.659-664.2003 pmid:12477868 pmcid:PMC140571 fatcat:kmnyugjhxfa2npcjjl4fhfsx6a

Three-dimensional structure of infectious bursal disease virus determined by electron cryomicroscopy

B Böttcher, N A Kiselev, V Y Stel'Mashchuk, N A Perevozchikova, A V Borisov, R A Crowther
1997 Journal of Virology  
The map shows that the structure of the virus is based on a T‫31؍‬ lattice and that the subunits are predominantly trimer clustered.  ...  From electron micrographs of frozen, hydrated, unstained specimens, we have computed a three-dimensional map of IBDV at about 2 nm resolution.  ...  ACKNOWLEDGMENTS This work was supported in part by grant 96-04-48894 from the Russian Foundation for Basic Science Investigations (N.A.K. and V.Y.S) and by an EEC Human Capital and Mobility grant (ERB4001  ... 
doi:10.1128/jvi.71.1.325-330.1997 fatcat:uqxso4p3mvd63ns7525j5urrpq

Three-dimensional structure of infectious bursal disease virus determined by electron cryomicroscopy

B Böttcher, N A Kiselev, V Y Stel'Mashchuk, N A Perevozchikova, A V Borisov, R A Crowther
1997 Journal of Virology  
The map shows that the structure of the virus is based on a T=13 lattice and that the subunits are predominantly trimer clustered.  ...  From electron micrographs of frozen, hydrated, unstained specimens, we have computed a three-dimensional map of IBDV at about 2 nm resolution.  ...  ACKNOWLEDGMENTS This work was supported in part by grant 96-04-48894 from the Russian Foundation for Basic Science Investigations (N.A.K. and V.Y.S) and by an EEC Human Capital and Mobility grant (ERB4001  ... 
pmid:8985353 pmcid:PMC191054 fatcat:32i2a5x4efcyforwdo2jeaxzxq

A low-resolution structure of rice dwarf virus determined byab initiophasing

Hisashi Naitow, Yukio Morimoto, Hiroshi Mizuno, Hiromi Kano, Toshihiro Omura, Mika Koizumi, Tomitake Tsukihara
1999 Acta Crystallographica Section D: Biological Crystallography  
The structure of rice dwarf virus was then solved with ab initio phasing up to 20 A Ê resolution.  ...  Using data synthesized from a rice dwarf virus model crystal in the space group I222, the possibility of ab initio phasing was thoroughly examined.  ...  TT was supported in part by the`Research for the Future' Program (No. 96L00503) from the Japan Society for the Promotion of Science, Grant-in-Aids for Science Research on Priority Area (No. 05244103 and  ... 
doi:10.1107/s0907444998007677 pmid:10089397 fatcat:vbdskshhozft5azqsua4ejxrru

Orthobunyavirus Ultrastructure and the Curious Tripodal Glycoprotein Spike

Thomas A. Bowden, David Bitto, Angela McLees, Christelle Yeromonahos, Richard M. Elliott, Juha T. Huiskonen, Ted C. Pierson
2013 PLoS Pathogens  
Whilst Bunyamwera virions are pleomorphic in shape, they display a locally ordered lattice of glycoprotein spikes.  ...  Using sub-tomogram averaging, we derived a three-dimensional model of the trimeric pre-fusion glycoprotein spike to 3-nm resolution.  ...  structure and the same structure rotated in one-degree increments around the long axis.  ... 
doi:10.1371/journal.ppat.1003374 pmid:23696739 pmcid:PMC3656102 fatcat:dozayxvp5famrkjc6ggmf677ba

A Surface Loop of the Potato Leafroll Virus Coat Protein Is Involved in Virion Assembly, Systemic Movement, and Aphid Transmission

L. Lee, I. B. Kaplan, D. R. Ripoll, D. Liang, P. Palukaitis, S. M. Gray
2004 Journal of Virology  
PLRV virions play key roles in phloem-limited virus movement in plant hosts as well as in transport and persistence in the aphid vectors.  ...  These results identified amino acid residues in a surfaceoriented loop of the coat protein that are critical for virus assembly and stability, systemic infection of plants, and movement of virus through  ...  P.P. is supported by a grant-in-aid from the Scottish Executive Environment and Rural Affairs Department to the SCRI.  ... 
doi:10.1128/jvi.79.2.1207-1214.2005 pmid:15613347 pmcid:PMC538549 fatcat:64eens4zv5hq3gxkvxsuwygaoy

Cryoelectron-Microscopy Image Reconstruction of Symmetry Mismatches in Bacteriophage φ29

Marc C. Morais, Yizhi Tao, Norman H. Olson, Shelley Grimes, Paul J. Jardine, Dwight L. Anderson, Timothy S. Baker, Michael G. Rossmann
2001 Journal of Structural Biology  
A method has been developed for three-dimensional image reconstruction of symmetry-mismatched components in tailed phages.  ...  of the stator in the 29 DNA packaging motor.  ...  We thank Cheryl Towell and Sharon Wilder for help in the preparation of the manuscript.  ... 
doi:10.1006/jsbi.2001.4379 pmid:11562164 pmcid:PMC5595366 fatcat:4kluevoklfa6npcuvp6n5brskq

C Terminus of Infectious Bursal Disease Virus Major Capsid Protein VP2 Is Involved in Definition of the T Number for Capsid Assembly

J. R. Caston, J. L. Martinez-Torrecuadrada, A. Maraver, E. Lombardo, J. F. Rodriguez, J. I. Casal, J. L. Carrascosa
2001 Journal of Virology  
IBDV is a nonenveloped virus that differs from most dsRNA viruses in having a single shell.  ...  We have computed three-dimensional maps of IBDV capsid and virus-like particles built up by VP2 alone by using electron cryomicroscopy and image-processing techniques.  ...  Hill (NIH) for assistance in setting up the computers, M. Cerritelli (NIH) for the gift of T4 bacteriophage, and L. Sánchez-Pulido (CNB) for VP2 secondary-structure prediction.  ... 
doi:10.1128/jvi.75.22.10815-10828.2001 pmid:11602723 pmcid:PMC114663 fatcat:necf7dq2vzha7jpcuoa3pc3bqi

Electron Cryo-Microscopy and Single-Particle Averaging of Rift Valley Fever Virus: Evidence for GN-GC Glycoprotein Heterodimers

J. T. Huiskonen, A. K. Overby, F. Weber, K. Grunewald
2009 Journal of Virology  
Here, we have studied the structure of RVFV using electron cryo-microscopy combined with three-dimensional reconstruction and single-particle averaging.  ...  Rift Valley fever virus (RVFV) is a member of the genus Phlebovirus within the family Bunyaviridae. It is a mosquito-borne zoonotic agent that can cause hemorrhagic fever in humans.  ...  .), and from the Deutsche Forschungsgemeinschaft (GR1990/1-3 and -2-1 to K.G. and WE 2616/5-1 to F.W.).  ... 
doi:10.1128/jvi.02483-08 pmid:19193794 pmcid:PMC2663282 fatcat:mp7g6qml3fevtilbu55jmusv7e

Structures of Coxsackievirus A10 unveil the molecular mechanisms of receptor binding and viral uncoating

Ling Zhu, Yao Sun, Jinyan Fan, Bin Zhu, Lei Cao, Qiang Gao, Yanjun Zhang, Hongrong Liu, Zihe Rao, Xiangxi Wang
2018 Nature Communications  
We also report two expanded structures of CVA10, an empty particle and uncoating intermediate at atomic resolution, as well as a medium-resolution genome structure reconstructed using a symmetry-mismatch  ...  The atomic-resolution structure of the CVA10 virion, which is within the KREMEN1-dependent subgroup, shows significant conformational differences in the putative receptor binding sites and serotype-specific  ...  Data availability The atomic coordinates of CVA10 mature virus, empty-and A-particles have been submitted to Protein Data Bank with accession numbers PDB: 6AKS, 6AKU, and 6AKT respectively.  ... 
doi:10.1038/s41467-018-07531-0 pmid:30478256 pmcid:PMC6255764 fatcat:fyx6pmuzzvh4hfs3i6fkm4zfzy

The protruding domain of the coat protein of Melon necrotic spot virus is involved in compatibility with and transmission by the fungal vector Olpidium bornovanus

Takehiro Ohki, Fusamichi Akita, Tomofumi Mochizuki, Ayami Kanda, Takahide Sasaya, Shinya Tsuda
2010 Virology  
Comparison of the three-dimensional structures between the CP of the two MNSV strains showed that many of the differences in these amino acid residues are present on the surface of the virus particles,  ...  of O. bornovanus and in the fungal transmission of the virus.  ...  The three-dimensional structures of CP of MNSV-W were predicted as for the analysis of MNSV CP (Wada et al., 2008) .  ... 
doi:10.1016/j.virol.2010.03.020 pmid:20381824 fatcat:4lo57uprpfdfbkwdufl22jqmua

Mechanism for maturation-related reorganization of flavivirus glycoproteins

Pavel Plevka, Anthony J. Battisti, Ju Sheng, Michael G. Rossmann
2014 Journal of Structural Biology  
Here we used a combination of three-dimensional cryo-electron tomography and two-dimensional image analysis to study pleomorphic maturation intermediates of dengue virus 2.  ...  Flaviviruses, such as dengue, West Nile, and yellow fever viruses, assemble as fusion-incompetent particles and subsequently undergo a large reorganization of their glycoprotein envelope resulting in formation  ...  The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.  ... 
doi:10.1016/j.jsb.2013.11.001 pmid:24252771 pmcid:PMC3901303 fatcat:afejz4577jebrfhaetwrku7q4u

Electron Cryomicroscopy of Biological Machines at Subnanometer Resolution

Wah Chiu, Matthew L. Baker, Wen Jiang, Matthew Dougherty, Michael F. Schmid
2005 Structure  
These steps include component segthe components within the machine are sometimes difmentation, averaging based on local symmetry among ferent from those in the isolated and crystallized forms. components  ...  The procapsid to capsid transition References in double-stranded DNA bacteriophages. In Structural Biology of Viruses, W. Chiu, R.M. Burnett, and R. L. Garcea, eds. (New York: Alberts, B. (1998).  ...  In determining the symmetry axis and symogous structures.  ... 
doi:10.1016/j.str.2004.12.016 pmid:15766537 fatcat:7q33fiifdvevxohmpkmey2om4m

Automated segmentation of molecular subunits in electron cryomicroscopy density maps

Matthew L. Baker, Zeyun Yu, Wah Chiu, Chandrajit Bajaj
2006 Journal of Structural Biology  
This method is applicable to object with and without symmetry and takes advantage of global and local symmetry axes if present.  ...  density and available structures of the components in the PDB.  ...  Detected global icosahedral symmetry and local 6-fold symmetry axes are shown in (B). Two segmented capsomeres are shown in Fig. 3 . 3 Segmentation of GroEL at different resolutions.  ... 
doi:10.1016/j.jsb.2006.05.013 pmid:16908194 fatcat:zroh7d6tlrejpenqsfk6lcygku

Conformational Reorganization of the SARS Coronavirus Spike Following Receptor Binding: Implications for Membrane Fusion

Daniel R. Beniac, Shauna L. deVarennes, Anton Andonov, Runtao He, Tim F. Booth, Olivier Schwartz
2007 PLoS ONE  
We have shown that ACE2 binding results in structural changes that appear to be the initial step in viral membrane fusion, and precisely localized the receptor-binding and fusion core domains within the  ...  Previous structural studies of membrane fusion proteins have largely used crystallography of static molecular fragments, in isolation of their transmembrane domains.  ...  The receptor-binding domain is localized in a position on the distal end of the molecule, closer to the 3-fold axis than anticipated, yet still in a position that would not impede these structural re-arrangements  ... 
doi:10.1371/journal.pone.0001082 pmid:17957264 pmcid:PMC2034598 fatcat:wfhetngsfba3ddak325hshv6cu
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