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Building Distinct Actin Filament Networks in a Common Cytoplasm

Alphée Michelot, David G. Drubin
2011 Current Biology  
Eukaryotic cells generate a diversity of actin filament networks in a common cytoplasm to optimally perform functions such as cell motility, cell adhesion, endocytosis and cytokinesis.  ...  in a common cytoplasm that contains a complex mixture of all the proteins?  ...  A variety of studies suggest that upon nucleation, actin filaments adopt a certain conformation, which favors binding by a specific subset of ABPs.  ... 
doi:10.1016/j.cub.2011.06.019 pmid:21783039 pmcid:PMC3384529 fatcat:dk2242z3xzahzl4lhg7q4lf6r4

Cargo licenses maturation of stalled endocytic sites through a regulatory checkpoint [article]

Ross TA Pedersen, Julian Edwin Hassinger, Paul Marchando, David G. Drubin
2019 bioRxiv   pre-print
Studies of Saccharomyces cerevisiae traced a detailed timeline of assembly of the CME machinery.  ...  During Clathrin-mediated endocytosis (CME), over 50 different proteins assemble on the plasma membrane to reshape it into a cargo-laden vesicle.  ...  (Sun and Drubin, 2012) .  ... 
doi:10.1101/834523 fatcat:chu2gxjbzzbb3jmauzbrnodb4m

Crafting a career in molecular animation

Janet Iwasa, David G. Drubin
2014 Molecular Biology of the Cell  
Three-dimensional animation software is well known for Monitoring Editor David G. Drubin University of California, Berkeley Molecular Biology of the Cell Discovery Corps.  ...  This was important, because I had only 2 years to complete more than a dozen animations, a website, and a museum exhibit.  ... 
doi:10.1091/mbc.e14-01-0699 pmid:25267313 pmcid:PMC4230579 fatcat:bvmjb6dqivb3rebrtkcn2femzy

A Modular Design for the Clathrin- and Actin-Mediated Endocytosis Machinery

Marko Kaksonen, Christopher P. Toret, David G. Drubin
2005 Cell  
Endocytosis depends on an extensive network of interacting proteins that execute a series of distinct subprocesses.  ...  Previously, we used live-cell imaging of six budding-yeast proteins to define a pathway for association of receptors, adaptors, and actin during endocytic internalization.  ...  This work was supported by NIH grants R01GM50399 and R01GM42759 and NIH Shared Instrumentation Grant S10-RR019290 to D.G.D. and by a Sigrid Jusélius Foundation and Finnish Academy fellowship to M.K.  ... 
doi:10.1016/j.cell.2005.09.024 pmid:16239147 fatcat:pqicgakimbdvrjwhekhdggw35m

So you want to be a science writer

John Fleischman, Christina Szalinski, David G. Drubin
2014 Molecular Biology of the Cell  
For those thinking of a career in science writing, here is a flash history, a quick overview, some advice, and a few cautions.  ...  A television reporter popped up in his best blazer, microphone flag, and booming baritone to ask, "So, doc, when will Man walk on Venus?" The JPL panel was speechless for a long moment.  ...  Today, Americans are as Monitoring Editor David G. Drubin University of California, Berkeley ASCB, of which I was a member, was looking for a PhD cell biologist to write for them.  ... 
doi:10.1091/mbc.e14-04-0857 pmid:24970484 pmcid:PMC4072566 fatcat:esmwnd2l6fbrffgh7g3smhgqfa

Aip1p Interacts with Cofilin to Disassemble Actin Filaments

Avital A. Rodal, Jonathan W. Tetreault, Pekka Lappalainen, David G. Drubin, David C. Amberg
1999 Journal of Cell Biology  
Actin interacting protein 1 (Aip1) is a conserved component of the actin cytoskeleton first identified in a two-hybrid screen against yeast actin.  ...  A two-hybrid-based approach using cofilin and actin mutants identified residues necessary for the interaction of actin, cofilin, and Aip1p in an apparent ternary complex.  ...  We thank Steve Elledge, Fred Winston, and David Botstein for providing strains.  ... 
doi:10.1083/jcb.145.6.1251 pmid:10366597 pmcid:PMC2133144 fatcat:2e5lcnelf5azpbpax65raxmjbu

Clathrin-mediated endocytosis in budding yeast at a glance

Rebecca Lu, David G. Drubin, Yidi Sun
2016 Journal of Cell Science  
In this Cell Science at a Glance article and accompanying poster, we review our current knowledge of the timeline of endocytic site maturation and discuss recent works focusing on how phosphorylation,  ...  , in CME site initiation (Sun and Drubin, 2013) .  ...  coat proteins do not internalize, but actin continuously streams inwards from these coat proteins (Sun and Drubin, 2013) .  ... 
doi:10.1242/jcs.182303 pmid:27084361 pmcid:PMC4852772 fatcat:ipchen5rijgenbbw3qvjkm4pve

A Pathway for Association of Receptors, Adaptors, and Actin during Endocytic Internalization

Marko Kaksonen, Yidi Sun, David G. Drubin
2003 Cell  
, attempts to show a direct linkage between these two sys-teins.  ...  Actin polymerization occurs late in the endocytic pathway and is required both for endocytic gested to function in endocytosis (Engqvist-Goldstein and Drubin, 2003; Geli and Riezman, 1998; Munn, 2001).  ...  and dynamin in yeast endocytosis, a similar sequence of events, in which actin rate of patch motility (Belmont and Drubin, 1998; Lappa-lainen and .  ... 
doi:10.1016/s0092-8674(03)00883-3 pmid:14622601 fatcat:vhcdjawoeze5ta2jjefd266xle

Stem cells: the new "model organism"

David G. Drubin, Anthony A. Hyman, Doug Kellogg
2017 Molecular Biology of the Cell  
Human tissue culture cells have long been a staple of molecular and cell biology research.  ...  Here we argue that combined advances in genome editing, stem cell production, and organoid derivation from stem cells represent a revolution in cell biology.  ...  Chromosomal aberrations are a problem because they result in abnormal gene expression. Chromosome instability is a problem because cellular phenotypes are unstable.  ... 
doi:10.1091/mbc.e17-03-0183 pmid:28559439 pmcid:PMC5449140 fatcat:tpdzazue65bdpcun3l2f3tcgkm

Experimentalist Meets Theoretician: A Tale of Two Scientific Cultures

David G. Drubin, George Oster, Doug Kellogg
2010 Molecular Biology of the Cell  
This is the story of how a small team of experimentalists and theoreticians collaborated to develop a theoretical model for vesicle formation during endocytosis.  ...  During the evolution of our theoretical model, we educated each other, organized our thoughts and our data, developed a conceptual framework for understanding the mechanochemistry of endocytosis, and generated  ...  Detailed quantitative microscopy and genetic studies had enabled the Drubin laboratory to acquire an enormous "pile of facts" describing the order and timing of events along the endocytic pathway in budding  ... 
doi:10.1091/mbc.e10-02-0143 pmid:20444974 pmcid:PMC2893975 fatcat:2j375qjexbawdbgnbsh6gwmi4e

A statistical anomaly indicates symbiotic origins of eukaryotic membranes

Suneyna Bansal, Aditya Mittal, David G. Drubin
2015 Molecular Biology of the Cell  
In this work, rigorous compositional analyses of ∼5000 plasma membrane lipid constituents of 273 species in the three life domains (archaea, eubacteria, and eukaryotes) revealed a remarkable statistical  ...  (A) Representation of a generic chemical structure for a membrane lipid composed of two regions: a hydrophilic part made up of polar atoms and a hydrophobic part composed of nonpolar atoms or carbon tails  ...  Archaea consisted of a total of 11 different classes with 34 different membrane lipid compositions, eubacteria contained a total of 14 classes with 45 compositions, and eukaryotes consisted of a total  ... 
doi:10.1091/mbc.e14-06-1078 pmid:25631820 pmcid:PMC4454172 fatcat:2y5bofzqfza45lpxt2xhkf5eha

T-cells play the classics with a different spin

Michael L. Dustin, David G. Drubin
2014 Molecular Biology of the Cell  
In a second example, lymphocytes have their own subfamily of integrins, the β2 subfamily, and only integrins in this family form a stable adhesion ring using freely mobile ligands, a key feature of the  ...  It is unusual for the ESCRT pathway to operate at the plasma membrane, but this may allow a novel form of cell-cell communication by providing a multivalent ligand for major histocompatibility complex-peptide  ...  ., 2012; Figure 1, A and B) .  ... 
doi:10.1091/mbc.e13-11-0636 pmid:24874016 pmcid:PMC4038497 fatcat:sji4geq7ivhnbbjjzzkhhxotay

A role for the yeast actin cytoskeleton in pheromone receptor clustering and signalling

Kathryn R. Ayscough, David G. Drubin
1998 Current Biology  
Numerous proteins have been identified in yeast that accumulate both at a position on the cell cortex that will develop into a mating projection in response to pheromone binding and at the site of bud  ...  When the actin cytoskeleton is disrupted before bud formation by the addition of latrunculin-A (LAT-A), several proteins involved in budding, including the GTPase Cdc42p, are still able to achieve their  ...  This work was supported by a Career Development Fellowship from the Wellcome Trust to K.R.A. and grants from the National Institutes of Health and the American Cancer Society to D.D.  ... 
doi:10.1016/s0960-9822(07)00374-0 pmid:9707405 fatcat:e2r7eidmhfgcpeug6nzjgf4awu

Isolation and partial purification of theSaccharomyces cerevisiaecytokinetic apparatus

Brian A. Young, Christopher Buser, David G. Drubin
2009 Cell Motility and the Cytoskeleton  
Cytokinesis is the process by which a cell physically divides in two at the conclusion of a cell cycle.  ...  This first successful isolation of the cytokinetic apparatus from a genetically tractable organism promises to make possible a deeper understanding of cytokinesis.  ...  Supplementary Material Refer to Web version on PubMed Central for supplementary material. of the Drubin and Barnes laboratories for useful discussions and experimental advice.  ... 
doi:10.1002/cm.20412 pmid:19790107 pmcid:PMC2825282 fatcat:4ucz3avxvra7jiidwazsafwimm

Blood-based identification of non-responders to anti-TNF therapy in rheumatoid arthritis

Ty M. Thomson, Reynald M. Lescarbeau, David A. Drubin, Daphna Laifenfeld, David de Graaf, David A. Fryburg, Bruce Littman, Renée Deehan, Aaron Van Hooser
2015 BMC Medical Genomics  
nearly a third of non-responders.  ...  Conclusions: We were able to successfully produce a classifier to identify a population of RA patients significantly enriched in anti-TNF non-responders across four different patient cohorts.  ...  Acknowledgements The authors would like to thank Natalie Catlett for her critical review of the manuscript, Mathukumalli Vidyasagar for a helpful feedback on the manuscript and the methodologies employed  ... 
doi:10.1186/s12920-015-0100-6 pmid:26036272 pmcid:PMC4455917 fatcat:xsy3tp6h5jdkjdgewg5bzd6bqi
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