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Hedera helix L

DANIEL J. METCALFE
2005 Journal of Ecology  
Acknowledgements I am indebted to George Metcalfe for stem sections, David Roy for access to the Phytophagous Insects Database, Lawren Sack from translating from French, Susan Solbrå for translating from  ...  Metcalfe and L.  ...  Metcalfe and L. Sack, unpublished data).  ... 
doi:10.1111/j.1365-2745.2005.01021.x fatcat:7x6xr652gbasdg5ujhxpetanbm

Leaf chemical and spectral diversity in Australian tropical forests

Gregory P. Asner, Roberta E. Martin, Andrew J. Ford, Daniel J. Metcalfe, Michael J. Liddell
2009 Ecological Applications  
and J is the total population of species.  ...  sample population divided by the minimum value of the population: a ¼ X X ij ¼ X x ij À MINðx iJ Þ MINðx iJ Þ ð1Þ where X ij is the standardized leaf property for each leaf constituent i and species j,  ... 
doi:10.1890/08-0023.1 pmid:19323186 fatcat:qvmsylgwprc3tmxxe7z2ubuz6e

Temporal cloaking for data suppression and retrieval

Joseph M. Lukens, Andrew J. Metcalf, Daniel E. Leaird, Andrew M. Weiner
2014 Optica  
Recent research on time cloaking has revealed a fascinating approach to hide temporal events from an interrogating optical field, by opening up and subsequently closing intensity gaps in a probe beam. Experiments thus far have demonstrated temporal cloaking of nonlinear interactions and high-speed optical data. Here we report a temporal cloak with the new capability not only to hide optical data, but also to concurrently transmit it along another wavelength channel for subsequent readout,
more » ... g the information from one observer while directing it to another. Additionally, the cloak succeeds in passing modulated data unscathed through a scrambling event, providing a new form of tampering resistance. Both examples launch a paradigm shift in temporal cloaking: instead of using time cloaks primarily to disrupt communication, we show how they can also improve data transmission, in turn greatly widening the range of possible applications in telecommunications.
doi:10.1364/optica.1.000372 fatcat:emuk5uo5unhzfohsmumnjcefty

Autophagy and misfolded proteins in neurodegeneration

Daniel J. Metcalf, Moisés García-Arencibia, Warren E. Hochfeld, David C. Rubinsztein
2012 Experimental Neurology  
Metcalf et al. / Experimental Neurology 238 (2012) 22-28  ... 
doi:10.1016/j.expneurol.2010.11.003 pmid:21095248 pmcid:PMC3463804 fatcat:iguargfwnbbtrb2ye3mzcfpqpa

Blind assessment of localisation microscope image resolution

Eric J Rees, Miklos Erdelyi, Dorothea Pinotsi, Alex Knight, Daniel Metcalf, Clemens F Kaminski
2012 Optical Nanoscopy  
ð Þ ¼ Σ n j¼1 1 h 2 j K x À x j h j ð7Þ In the model scenario of "frequent" localisations, n → ∞ and ρ s converges in distribution towards ρ m , i.e. the localisations perfectly map out the measurement  ...  In Gaussian Visualisation K is a 2dimensional Gaussian, h j scales its width to suit the local sampling density, and h j -2 normalises the weight of each localisation in the reconstructed image. ρ v x  ... 
doi:10.1186/2192-2853-1-12 fatcat:c6oad4o4kjf2dn4z6zsbc5boza

Test Samples for Optimizing STORM Super-Resolution Microscopy

Daniel J. Metcalf, Rebecca Edwards, Neelam Kumarswami, Alex E. Knight
2013 Journal of Visualized Experiments  
STORM is a recently developed super-resolution microscopy technique with up to 10 times better resolution than standard fluorescence microscopy techniques. However, as the image is acquired in a very different way than normal, by building up an image molecule-by-molecule, there are some significant challenges for users in trying to optimize their image acquisition. In order to aid this process and gain more insight into how STORM works we present the preparation of 3 test samples and the
more » ... logy of acquiring and processing STORM super-resolution images with typical resolutions of between 30-50 nm. By combining the test samples with the use of the freely available rainSTORM processing software it is possible to obtain a great deal of information about image quality and resolution. Using these metrics it is then possible to optimize the imaging procedure from the optics, to sample preparation, dye choice, buffer conditions, and image acquisition settings. We also show examples of some common problems that result in poor image quality, such as lateral drift, where the sample moves during image acquisition and density related problems resulting in the 'mislocalization' phenomenon. Video Link The video component of this article can be found at https://www.jove.com/video/50579/ 21 . They have since been shown to be applicable to a number of different dyes [28] [29] [30] , which makes the methodology particularly attractive because of the specificity and relative convenience of well-established immunolabeling approaches to perform fluorescence microscopy. Consequently, there is easy access to a range of dye-antibody and dye-biomolecule conjugates, which have the potential to be used in localization-based super-resolution imaging. Whilst the general principles of STORM and similar approaches are relatively simple, and at first sight the hardware and sample preparation seem relatively straightforward, there are a number of steps in the process which are critical to achieving high quality, artifact-free, super-resolution images. As with all microscopy techniques the process can be thought of in 3 main steps: first, sample preparation, second, image acquisition and, third, image processing and/or image analysis and interpretation. The additional resolving power and method of generating super-resolution images using a localization-based approach introduces some new problems and considerations [31] [32] [33]
doi:10.3791/50579 pmid:24056752 pmcid:PMC3857894 fatcat:s3nqxllrfjaq3na3xtl7nmywyq

A non-canonical arm of UPRER mediates longevity through ER remodeling and lipophagy [article]

Joseph R Daniele, Ryo Higuchi-Sanabria, Vidhya Ramachandran, Melissa Sanchez, Jenni Durieux, Sarah U Tronnes, Joseph W Paul, Daniel J Esping, Samira Monshietehadi, Melissa G Metcalf, Andrew Dillin
2018 bioRxiv   pre-print
ACKNOWLEDGEMENTS We are grateful to Lindsay Daniele for her figure illustrations and to Raz Bar-Ziv, Hope Henderson, Andrew Murley, and Ashley Frakes for careful reading of the manuscript and helpful suggestions  ...  Zhang, P., Na, H., Liu, Z., Zhang, S., Xue, P., Chen, Y., Pu, J., Peng, G., Huang, X., Yang, F., et al. (2012) .  ...  Biosorter calibration, cleaning, and sample running were performed as described in (Daniele et al., 2017) .  ... 
doi:10.1101/471177 fatcat:bpnzpsxbxfbobihh2t7ro6f2ca

Identifying Priority Areas for Conservation and Management in Diverse Tropical Forests

Karel Mokany, David A. Westcott, Soumya Prasad, Andrew J. Ford, Daniel J. Metcalfe, Bruno Hérault
2014 PLoS ONE  
We then calculate the predicted Sorensen's compositional dissimilarity between the two grid cells (b ij = 1-[2S com,ij /(S i +S j )]) using the predicted species richness of each grid cell (S i , S j )  ...  The Australian Wet Tropics flora has particular value globally for the large representation of ''primitive'' angiosperm families (13 out of 28) as described by Metcalfe and Ford [6, 39] .  ... 
doi:10.1371/journal.pone.0089084 pmid:24551222 pmcid:PMC3925232 fatcat:x37f4vuup5cytkllw7mhlb3sie

Cross-Media Transfers of Hazardous Wastes

Gilbert E. Metcalf, Daniel J. Dudek, Cleve E. Willis
1984 Northeastern Journal of Agricultural and Resource Economics  
Extensive research in waste reduction techniques for electroplating operations exists (see Metcalf, Willis and Dudek [ 1984] for references).  ...  Figure 1 presents a stylized tableau of the process analysis model which is described in greater detail in Metcalf, Willis, and Dudek [1984] , The first constraint set simply states that the workpiece  ... 
doi:10.1017/s0899367x00000507 fatcat:xkmppotckjajlhk5rcascxgdaq

A Global Immunological Observatory to meet a time of pandemics

Michael J Mina, C Jessica E Metcalf, Adrian B McDermott, Daniel C Douek, Jeremy Farrar, Bryan T Grenfell
2020 eLife  
GIO would address all of these gaps (Metcalf et al. 2017 (Metcalf et al. , 2016 as with detailed analysis of their T cell responses.  ...  SARS-CoV-2 in a timely fashion 45 in almost any setting. 46 47 How could we better deploy and refine tools for sophisticated pathogen surveillance to better 48 meet likely future comparable threats (Metcalf  ... 
doi:10.7554/elife.58989 pmid:32510329 pmcid:PMC7292646 fatcat:kwgzs2hdkzgstkj3gilw66jmhy

A Two-Tier Golgi-Based Control of Organelle Size Underpins the Functional Plasticity of Endothelial Cells

Francesco Ferraro, Janos Kriston-Vizi, Daniel J. Metcalf, Belen Martin-Martin, Jamie Freeman, Jemima J. Burden, David Westmoreland, Clare E. Dyer, Alex E. Knight, Robin Ketteler, Daniel F. Cutler
2014 Developmental Cell  
(J) Golgi-positive structures (TGN46 positive) in siRNA-treated cells incubated overnight with DMSO or nocodazole and analyzed by HTM. For each treatment, n = $10 5 ; ###p < 10 À15 .  ...  WPBs store endothelial von Willebrand factor (vWF), a large glycoprotein that undergoes complex processing along the secretory pathway (Metcalf et al., 2008) .  ... 
doi:10.1016/j.devcel.2014.03.021 pmid:24794632 pmcid:PMC4022834 fatcat:brt35jg7qrdvhhxabamcf5ha34

Physiology, Behavior, and Conservation

Steven J. Cooke, Daniel T. Blumstein, Richard Buchholz, Tim Caro, Esteban Fernández-Juricic, Craig E. Franklin, Julian Metcalfe, Constance M. O'Connor, Colleen Cassady St. Clair, William J. Sutherland, Martin Wikelski
2014 Physiological and Biochemical Zoology  
Physiological knowledge is also increasingly being used to inform resource management of exploited species Metcalfe et al. 2012) .  ...  Several books have been written on analyzing behavioral data (e.g., Russo 2003; Blumstein and Daniel 2007; Martin and Bateson 2007) , and some animal behavior journals (e.g., Animal Behaviour) provide  ... 
doi:10.1086/671165 pmid:24457917 fatcat:sivuoudacnhn7g6nsqkayw2fia

Incomplete Penetrance of Population-Based Genetic Screening Results in Electronic Health Record

Gai Elhanan, Daniel Kiser, Iva Neveux, Shaun Dabe, Alexandre Bolze, William J. Metcalf, James T. Lu, Joseph J. Grzymski
2022 Frontiers in Genetics  
The clinical value of population-based genetic screening projects depends on the actions taken on the findings. The Healthy Nevada Project (HNP) is an all-comer genetic screening and research project based in northern Nevada. HNP participants with CDC Tier 1 findings of hereditary breast and ovarian cancer syndrome (HBOC), Lynch syndrome (LS), or familial hypercholesterolemia (FH) are notified and provided with genetic counseling. However, the HNP subsequently takes a "hands-off" approach: it
more » ... the responsibility of notified participants to share their findings with their healthcare providers, and providers are expected to implement the recommended action plans. Thus, the HNP presents an opportunity to evaluate the efficiency of participant and provider responses to notification of important genetic findings, using electronic health records (EHRs) at Renown Health (a large regional hospital in northern Nevada). Out of 520 HNP participants with findings, we identified 250 participants who were notified of their findings and who had an EHR. 107 of these participants responded to a survey, with 76 (71%) indicating that they had shared their findings with their healthcare providers. However, a sufficiently specific genetic diagnosis appeared in the EHRs and problem lists of only 22 and 10%, respectively, of participants without prior knowledge. Furthermore, review of participant EHRs provided evidence of possible relevant changes in clinical care for only a handful of participants. Up to 19% of participants would have benefited from earlier screening due to prior presentation of their condition. These results suggest that continuous support for both participants and their providers is necessary to maximize the benefit of population-based genetic screening. We recommend that genetic screening projects require participants' consent to directly document their genetic findings in their EHRs. Additionally, we recommend that they provide healthcare providers with ongoing training regarding documentation of findings and with clinical decision support regarding subsequent care.
doi:10.3389/fgene.2022.866169 pmid:35571025 pmcid:PMC9091193 fatcat:3isenzhhjzdfxb54lu5snwjg4q

The Visualization of Biofilms in Chronic Diabetic Foot Wounds Using Routine Diagnostic Microscopy Methods

Angela Oates, Frank L. Bowling, Andrew J. M. Boulton, Philip G. Bowler, Daniel G. Metcalf, Andrew J. McBain
2014 Journal of Diabetes Research  
Diabetic foot wounds are commonly colonised by taxonomically diverse microbial communities and may additionally be infected with specific pathogens. Since biofilms are demonstrably less susceptible to antimicrobial agents than are planktonic bacteria, and may be present in chronic wounds, there is increasing interest in their aetiological role. In the current investigation, the presence of structured microbial assemblages in chronic diabetic foot wounds is demonstrated using several
more » ... n methods. Debridement samples, collected from the foot wounds of diabetic patients, were histologically sectioned and examined using bright-field, fluorescence, and environmental scanning electron microscopy and assessed by quantitative differential viable counting. All samples (n= 26) harboured bioburdens in excess of 5 log10CFU/g. Microcolonies were identified in 4/4 samples by all three microscopy methods, although bright-field and fluorescence microscopy were more effective at highlighting putative biofilm morphology than ESEM. Results in this pilot study indicate that bacterial microcolonies and putative biofilm matrix can be visualized in chronic wounds using florescence microscopy and ESEM, but also using the simple Gram stain.
doi:10.1155/2014/153586 pmid:24839608 pmcid:PMC4009286 fatcat:fqdql3nrizbm3db3xxxoo5b6ui

Prophage WO genes recapitulate and enhance Wolbachia-induced cytoplasmic incompatibility

Daniel P. LePage, Jason A. Metcalf, Sarah R. Bordenstein, Jungmin On, Jessamyn I. Perlmutter, J. Dylan Shropshire, Emily M. Layton, Lisa J. Funkhouser-Jones, John F. Beckmann, Seth R. Bordenstein
2017 Nature  
-J. obtained the wVitA transcriptome.  ...  J. Invertebr. Pathol. 67, 55-64 (1996). 24. Wright, J. D. & Barr, A. R. Wolbachia and the normal and incompatible eggs of Aedes polynesiensis (Diptera: Culicidae). J. Invertebr.  ...  Jaenike, J., Dyer, K. A., Cornish, C. & Minhas, M. S. Asymmetrical reinforcement and Wolbachia infection in Drosophila. PLoS Biol. 4, e325 (2006). 29. Bordenstein, S. R., O'Hara, F. P. & Werren, J.  ... 
doi:10.1038/nature21391 pmid:28241146 pmcid:PMC5358093 fatcat:ue5vrpuynvat3mw2qlrhcx2uwq
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