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DIC image reconstruction using an energy minimization framework to visualize optical path length distribution

Krisztian Koos, József Molnár, Lóránd Kelemen, Gábor Tamás, Peter Horvath
2016 Scientific Reports  
We propose a variational framework for DIC image reconstruction.  ...  Differential interference contrast (DIC) is a qualitative microscopy technique that shows the optical path length differences within a specimen.  ...  Results In this section we describe our variational framework 19, 20 for DIC image reconstruction.  ... 
doi:10.1038/srep30420 pmid:27453091 pmcid:PMC4958949 fatcat:3xq7zh62tzfsbld4obczuo4gga

Nonnegative Mixed-Norm Preconditioning for Microscopy Image Segmentation [chapter]

Kang Li, Takeo Kanade
2009 Lecture Notes in Computer Science  
We propose a general algebraic framework for preconditioning microscopy images.  ...  The algorithms were extensively validated on simulated differential interference (DIC) microscopy images and challenging real DIC images of cell populations.  ...  Hoeppner (NINDS) for providing DIC images of CNS stem cells with ground truth.  ... 
doi:10.1007/978-3-642-02498-6_30 fatcat:zbtqjyndyndrxg7g3hkgbnstcm

Cell Membrane Tracking in Living Brain Tissue Using Differential Interference Contrast Microscopy

John Lee, Ilya Kolb, Craig R. Forest, Christopher J. Rozell
2018 IEEE Transactions on Image Processing  
As a secondary contribution, this paper also describes new community infrastructure in the form of a MATLAB toolbox for accurately simulating DIC microscopy images of in vitro brain slices.  ...  ., ‖·‖ 1 ) reconstruction approximations motivated Li and Kanade to develop a mixed-norm pre-conditioning approach [20] that exploited sparsity and smoothness in the OPL image using ℓ 1 -norm and total  ...  DIC microscopy images.  ... 
doi:10.1109/tip.2017.2787625 pmid:29346099 pmcid:PMC5839128 fatcat:lfj5xhcvdndrxi5rgreikyqsdi

Dynamic DIC by digital holography microscopy for enhancing phase-contrast visualization

Lisa Miccio, Andrea Finizio, Roberto Puglisi, Donatella Balduzzi, Andrea Galli, Pietro Ferraro
2011 Biomedical Optics Express  
In fact, the technique presented here, is useful for floating samples since it allows, from a single recording, to set a posteriori the best conditions for DIC imaging in conjunction with the numerical  ...  Differential image contrast (DIC), through the numerical managing and manipulation of complex wavefronts obtained by digital holography (DH), is investigated.  ...  In this way a novel concept of DIC in microscopy can be envisaged by using DH, we named here DDHIC (Dynamical Differential Holographic Image Contrast) where the numerical implementation of DIC images are  ... 
doi:10.1364/boe.2.000331 pmid:21339878 pmcid:PMC3038448 fatcat:usra4bprg5aerlgaggm63aoq3q

Alternating minimization algorithm for quantitative differential-interference contrast (DIC) microscopy

Joseph A. O'Sullivan, Chrysanthe Preza, Charles A. Bouman, Eric L. Miller, Ilya Pollak
2008 Computational Imaging VI  
A new iterative algorithm for quantitative image reconstruction for differential-interference contrast (DIC) microscopy is presented, along with simulations demonstrating key properties of the algorithm  ...  The algorithm is an alternating minimization (AM) algorithm based on a diffraction imaging model for DIC images.  ...  quantitative DIC microscope and for stimulating discussions.  ... 
doi:10.1117/12.785471 dblp:conf/cimaging/OSullivanP08 fatcat:veuocxbckvglbeun3bgh3asqiy

Review of quantitative phase-digital holographic microscopy: promising novel imaging technique to resolve neuronal network activity and identify cellular biomarkers of psychiatric disorders

Pierre Marquet, Christian Depeursinge, Pierre J. Magistretti
2014 Neurophotonics  
Quantitative phase microscopy (QPM) has recently emerged as a new powerful quantitative imaging technique well suited to noninvasively explore a transparent specimen with a nanometric axial sensitivity  ...  In this review, we expose the recent developments of quantitative phase-digital holographic microscopy (QP-DHM).  ...  Marquet, Depeursinge, and Magistretti: Review of quantitative phase-digital holographic microscopy: promising novel imaging. . .  ... 
doi:10.1117/1.nph.1.2.020901 pmid:26157976 pmcid:PMC4478935 fatcat:wjvtnf6fifbhxdgudvt245w5lm

Generative Spatiotemporal Modeling Of Neutrophil Behavior [article]

Narita Pandhe, Balazs Rada, Shannon Quinn
2018 arXiv   pre-print
interference contrast (DIC) microscopy.  ...  Alternatively, generative models learn the underlying distribution of the data, creating holistic representations that can be used in learning.  ...  We use GANs for DIC microscopy images consisting of a single channel.  ... 
arXiv:1804.00393v1 fatcat:3x4dpafrerb5ldcnpxbvxf52xi

Smart computational light microscopes (SCLMs) of smart computational imaging laboratory (SCILab)

Yao Fan, Jiaji Li, Linpeng Lu, Jiasong Sun, Yan Hu, Jialin Zhang, Zhuoshi Li, Qian Shen, Bowen Wang, Runnan Zhang, Qian Chen, Chao Zuo
2021 PhotoniX  
AbstractComputational microscopy, as a subfield of computational imaging, combines optical manipulation and image algorithmic reconstruction to recover multi-dimensional microscopic images or information  ...  Translating those concepts to stand-alone optical instruments for practical use is an essential step for the promotion and adoption of computational microscopy by the wider bio-medicine, industry, and  ...  Of course, it has not been a smooth journey.  ... 
doi:10.1186/s43074-021-00040-2 fatcat:p22txdl7ubeidnwxiuaoj7udxi

Super Resolution Digital Image Correlation (SR-DIC): an Alternative to Image Stitching at High Magnifications

R. S. Hansen, D. W. Waldram, T. Q. Thai, R. B. Berke
2021 Experimental mechanics  
Second, an applied translation is compared against DIC measurement using both low- and super-resolution images.  ...  Methods First, an image reconstruction test is performed, comparing the ability of three previously published SR algorithms to replicate a high-resolution image.  ...  They are fairly representative of SR capabilities and provide a framework with which to analyze the application of SR imaging for DIC measurements.  ... 
doi:10.1007/s11340-021-00729-2 fatcat:k7zwbxrlcfb77n7xdomtjcxmpy

One-Shot phase-recovery using a Cellphone RGB Camera on a Jamin-Lebedeff Microscope [article]

Benedict Diederich, Barbora Marsikova, Brad Amos, Rainer Heintzmann
2019 bioRxiv   pre-print
Whilst a differential interference contrast (DIC) microscopy interferes an image with itself shifted by half a point spread function, the shear between the object and reference image in a JL-microscope  ...  The algorithm is calibrated using a reference image of a known phase object (e.g. optical fiber).  ...  Acknowledgments 352 We thank especially Jan Peychl from the MPI-CBG in Dresden for giving us the  ... 
doi:10.1101/765958 fatcat:z2pni7uufnhbbhbytbpicdwuqa

One-shot phase-recovery using a cellphone RGB camera on a Jamin-Lebedeff microscope

Benedict Diederich, Barbora Marsikova, Brad Amos, Rainer Heintzmann, Yuval Garini
2019 PLoS ONE  
Whilst a differential interference contrast (DIC) microscopy interferes an image with itself shifted by half a point spread function, the shear between the object and reference image in a JL-microscope  ...  The algorithm is calibrated using a reference image of a known phase object (e.g. optical fiber).  ...  Special thanks goes to Patrick McCall and Moritz Kreysing who helped us acquiring the reference images using the Q-Phase at the MPI-CBG in Dresden.  ... 
doi:10.1371/journal.pone.0227096 pmid:31891618 pmcid:PMC6938357 fatcat:wndla7mvkrh37lkd2aqqgidplq

Deconvolved Spatial Light Interference Microscopy for Live Cell Imaging

J. P. Haldar, Zhuo Wang, G. Popescu, Zhi-Pei Liang
2011 IEEE Transactions on Biomedical Engineering  
Index Terms-Constrained image reconstruction, deconvolution, live cell imaging, quantitative phase imaging (QPI), spatial light interference microscopy (SLIM).  ...  Spatial light interference microscopy (SLIM) is a recently developed method for the label-free imaging of live cells, using the quantitative optical path length through the sample as an endogenous source  ...  An alternative to using exogenous contrast agents is the use of phase-sensitive microscopy techniques like phase contrast (PC) [2] and differential interference contrast (DIC) microscopy [3] .  ... 
doi:10.1109/tbme.2011.2158003 pmid:21622067 pmcid:PMC3286342 fatcat:aw6h5rvk3ff3xnunyhw6pn6z7y

Weaving of biomineralization framework in rotaliid foraminifera: Implications for paleoenvironmental reconstructions

Yukiko Nagai, Katsuyuki Uematsu, Chong Chen, Ryoji Wani, Jarosław Tyszka, Takashi Toyofuku
2018 Biogeosciences Discussions  
In this study, the functional role of pseudopodial structures during chamber formation is elucidated by detailed observation of Ammonia beccarii (Linnaeus) using a time-lapse optical imaging system and  ...  high-resolution electron microscopy.  ...  This work was supported by a grant from the Faculty of Environment and Information Sciences, Yokohama National University (to YN) and a JSPS KAKENHI Grant  ... 
doi:10.5194/bg-2018-295 fatcat:yvtfbevtnzeutdy2dwimo63xua

Coupled digital image correlation and quasi-static elasticity imaging of inhomogeneous orthotropic composite structures

Danny Smyl, Kim-Niklas Antin, Dong Liu, Sven Bossuyt
2018 Inverse Problems  
from Digital Image Correlation (DIC).  ...  To address this, we propose an inverse method, based on Quasi-Static Elasticity Imaging (QSEI) for determining inhomogeneous orthotropic elastic properties using distributed displacement measurements obtained  ...  Figure 7 . 7 Example image of a displaced beam with sprayed DIC contrast pattern. Figure 8 . 8 Example of a displaced QSEI grid using an interpolated displacement field obtained using DIC.  ... 
doi:10.1088/1361-6420/aae793 fatcat:2v73yxspjfa67odv4smocenxia

Traction Force Microscopy for Noninvasive Imaging of Cell Forces

Jeffrey A Mulligan, François Bordeleau, Cynthia A Reinhart-King, Steven G Adie
2018 Advances in Experimental Medicine and Biology  
Traction force microscopy is a noninvasive technique that enables the in vitro imaging and quantification of cell forces.  ...  Utilizing expertise from a variety of disciplines, recent developments in traction force microscopy are enhancing the study of cell forces in physiologically relevant model systems, and hold promise for  ...  Acknowledgments Recent studies and results presented here were funded in part by a grant from the US National Institutes of Health (R21EB022927, Adie) and a Cornell Discovery and Innovation Research Seed  ... 
doi:10.1007/978-3-319-95294-9_15 pmid:30368759 pmcid:PMC6980301 fatcat:iks36fhkz5gmfhhvl3gdk3v5pi
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