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Containers and aggregates, mutators and isolates for reactive programming
2014
Proceedings of the Fifth Anuual Scala Workshop on - SCALA '14
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This paper presents a generic framework for reactive programming that extends first-class reactive values with the concept of lazy reactive containers, backed by several concrete implementations. ...
Our framework addresses concurrency by introducing reactive isolates. We show examples that our programming model is efficient and convenient to use. ...
We compared Reactive Collections against RxJava and Scala.React implementations on an Intel i5-2500 3.30 GHz
Acknowledgements We would like to thank Erik Meijer for the discussions we had, his useful ...
doi:10.1145/2637647.2637656
dblp:conf/ecoop/ProkopecHO14
fatcat:ai2dxqzoynbjnfjoo5p727urd4
DnaK/DnaJ chaperone system reactivates endogenous E coli thermostable FBP aldolase in vivo and in vitro; the effect is enhanced by GroE heat shock proteins
2001
Cell stress & chaperones (Print)
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The dnaK756 and dnaJ259 mutations had a negative effect on the reactivation of thermally inactivated Fda. ...
GroEL/GroES alone were inefficient in the resolubilization or reactivation of the heat-aggregated Fda. ...
Ż ylicz, and P. Lund for E coli strains and T. Kaczorowski for M13/T7 phage. We thank K. Liberek and M. Ż ylicz for generous gifts of purified DnaK, DnaJ, GroES, and GroEL proteins. ...
doi:10.1379/1466-1268(2001)006<0029:ddcsre>2.0.co;2
pmid:11525240
pmcid:PMC434380
fatcat:msm6d7fvtrbzbi3cmmtawowixm
An integrated view of p53 dynamics, function, and reactivation
2021
Current Opinion in Structural Biology
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Expression level alterations and mutational frequency implicates p53 in most human cancers. ...
In this review, we show how both computational and experimental methods have been used to provide an integrated view of p53 dynamics, function, and reactivation potential. ...
The authors thank Chloe (Xiaoyi) Ma at the University of Toronto for the figure illustrations. ...
doi:10.1016/j.sbi.2020.11.005
pmid:33401096
pmcid:PMC8985518
fatcat:p7j7yw4glrhwvmten5rlakbqgm
Antigen-Driven Oligoclonal Expansion of Tumor-Infiltrating B Cells in Infiltrating Ductal Carcinoma of the Breast
2002
Journal of Immunology
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Clones 1-35 and 2-26 contained no V gene mutations in comparison with germline. Clones 2-38 and 1-18 shared a single common mutation from germline. B and C, Patient 2, clonal groups 3, 5. ...
TIL-B IgG H chains contained somatic mutations that clustered in the Ag-contacting CDRs, as is observed in affinity-matured Abs (reviewed in Refs. 30 and 43) , and as was also seen in tumor-draining lymph ...
doi:10.4049/jimmunol.169.4.1829
pmid:12165506
fatcat:b52feks3rzd6hjpwatdiverlge
The Genetics of Development in Dictyostelium Discoideum
1982
Annual Review of Genetics
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For example, a strain isolated as Agg-proved to carry mutant alleles defining the loci devA and devB (98). ...
Mutations Affecting Aggregative Performance Mutations affecting call aggregation constitute the largest group among unselected survivors of mutagenesis (58) . ...
doi:10.1146/annurev.ge.16.120182.002125
pmid:6760801
fatcat:4vbql4fdybexli6svreqij6bxe
Analysis of Functional Domains of the Enterococcus faecalis Pheromone-Induced Surface Protein Aggregation Substance
2001
Journal of Bacteriology
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Pheromone-inducible aggregation substance (AS) proteins of Enterococcus faecalis are essential for highefficiency conjugation of the sex pheromone plasmids and also serve as virulence factors during host ...
Analysis of these insertions revealed a domain necessary for donor-recipient aggregation that extends further into the amino terminus of the protein than previously reported. ...
We thank Pat Cleary for reading the manuscript and Pat Schlievert, Helmut Hirt, and John McCormick for providing antibodies, other reagents, and helpful discussion. ...
doi:10.1128/jb.183.19.5659-5667.2001
pmid:11544229
pmcid:PMC95458
fatcat:45k23z6obzha5k46xf6ddj3gwi
Reactivation of FMR1 by CRISPR/Cas9-Mediated Deletion of the Expanded CGG-Repeat of the Fragile X Chromosome
2016
PLoS ONE
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We observed transcriptional reactivation in approximately 67% of the CRISPR cut hybrid colonies and in 20% of isolated human FXS iPSC colonies. ...
The reactivated cells produced FMRP and exhibited a decline in DNA methylation at the FMR1 locus. ...
We thank Oskar Laur for generating CRISPR plasmid constructs and Robert B. Isett for methylation profiling array, whose work was supported by the Emory Integrated Genomics Core (EIGC). ...
doi:10.1371/journal.pone.0165499
pmid:27768763
pmcid:PMC5074572
fatcat:egwjazcucralvn6cn5dko3wvgy
Conserved Distal Loop Residues in the Hsp104 and ClpB Middle Domain Contact Nucleotide-binding Domain 2 and Enable Hsp70-dependent Protein Disaggregation
2013
Journal of Biological Chemistry
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Conclusion: Distal loop does not project out into solution and Hsp104 and ClpB are tuned differently for Hsp70 collaboration. ...
How Hsp104 and ClpB coordinate polypeptide handover with Hsp70 to dissolve disordered protein aggregates is unknown. ...
Isolated MD Does Not Interact with Hsp70 or Inhibit Disaggregation in Trans-Another possibility for how specific distal loop mutations impair Hsp70 collaboration is that mutation in this region disrupts ...
doi:10.1074/jbc.m113.520759
pmid:24280225
pmcid:PMC3887210
fatcat:b2pniowdgjcf7bf3j5ok5lhv54
Single amino acid substitutions of alpha 1-antitrypsin that confer enhancement in thermal stability
1994
Journal of Biological Chemistry
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Characterization of various single amino acid substitutions at position 51 suggests that volume and flexibility of hydrophobic side chain at the site are critical factors for enhancing the stability of ...
The mutation site is particularly interesting in that some genetic variants mapped at adjacent positions were shown previously to cause aggregation of the polypeptides, while the Phe51-->Cys mutation decreased ...
Kim for helpful discussion and comments. We also thank Dr. F. William Studier for providing PET-8c plasmid and bacterial strains, Dr. David P. ...
pmid:8144550
fatcat:tdukwgd63ncorjjun6ean3ehuq
N-Terminal Domain of Yeast Hsp104 Chaperone Is Dispensable for Thermotolerance and Prion Propagation but Necessary for Curing Prions by Hsp104 Overexpression
2006
Genetics
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Inactivating Hsp104 cures cells of [PSI 1 ] and other amyloidlike yeast prions. Overexpressing Hsp104 also eliminates [PSI 1 ], presumably by completely resolubilizing prion aggregates. ...
Hsp104D147, engineered to lack the NTD, supported [PSI 1 ] and functioned normally in thermotolerance and protein disaggregation. ...
We thank Yury Chernoff (Georgia Institute of Technology) for plasmids and John Glover (University of Toronto) for plasmids and Hsp104 antibody. ...
doi:10.1534/genetics.106.056820
pmid:16582428
pmcid:PMC1526498
fatcat:kb7suvvz2vaonhigwhplxubykq
Dynamic Diagnosis of Familial Prion Diseases Supports the β2-α2 Loop as a Universal Interference Target
2011
PLoS ONE
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However they variably have impact on the thermodynamic stability and metabolism of PrP C and on the properties of PrP Sc aggregates. ...
of the patches for intermolecular recognition, as the disappearance of those for conversion inhibitors and the emergence of an interaction site at the b2-a2 loop region. ...
[14] , whereby the structural properties of the isolate a2-a3 region, which recapitulates the amyloidogenic features of the whole protein and contains the mutations herein studied, have been elucidated ...
doi:10.1371/journal.pone.0019093
pmid:21552571
pmcid:PMC3084259
fatcat:c2vtsbki5ncvbejqvn4flf2jeu
Mutation of Mycobacterium tuberculosis and Implications for Using Whole-Genome Sequencing for Investigating Recent Tuberculosis Transmission
2022
Frontiers in Public Health
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Tuberculosis (TB) control programs use whole-genome sequencing (WGS) of Mycobacterium tuberculosis (Mtb) for detecting and investigating TB case clusters. ...
However, the variable and sometimes long duration of latent infection, combined with uncertainty in the Mtb mutation rate during latency, can complicate interpretation of WGS results. ...
to disease, allowing the TB program to conclude a recent TB case is attributable to reactivation of latent infection. ...
doi:10.3389/fpubh.2021.790544
pmid:35096744
pmcid:PMC8793027
fatcat:gfyulphumvb6vggo7dme2ztbha
Overexpression of mutated Cu,Zn-SOD in neuroblastoma cells results in cytoskeletal change
2005
American Journal of Physiology - Cell Physiology
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Yoshitaka Ikeda for technical support and critical discussion. ...
of the Ministry of Education, Science, Culture, Sports, and Technology, and a Grant-in-Aid for Scientific Research (C) No. 15500237. ...
Cells were incubated in DMEM containing 10% FBS for 1 day and then incubated with or without serum for an additional 48 h. ...
doi:10.1152/ajpcell.00014.2004
pmid:15456693
fatcat:wrellzbdxjadrfhf7b54h3jh3m
Role of chaperones and ATP synthase in DNA gyrase reactivation in Escherichia coli stationary-phase cells after nutrient addition
2014
SpringerPlus
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Escherichia coli stationary-phase (SP) cells contain relaxed DNA molecules and recover DNA supercoiling once nutrients become available. ...
In these cells, the reactivation of DNA gyrase, which is a DNA topoisomerase type IIA enzyme, is responsible for the recovery of DNA supercoiling. ...
We also thank L Camarena and C Domenzain for assistance in preparing the Figures and I Pérez-Montfort for editorial assistance. ...
doi:10.1186/2193-1801-3-656
pmid:25485196
pmcid:PMC4230433
fatcat:xs2ja5amlrhtxjlclig6ts7vpy
Molecular Evolution of the Thermosensitive PAb1620 Epitope of Human p53 by DNA Shuffling
1999
Journal of Biological Chemistry
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For this purpose, we applied a recently developed random mutagenesis technique called DNA shuffling and screened for p53 variants that could retain reactivity to the native conformation-specific anti-p53 ...
Mutational analysis identified 20 amino acid residues in the core domain of p53 (amino acids 101-120) responsible for the thermostable phenotype. ...
Scott, and C. Stephen for helpful discussions and critical reading of the manuscript. We also thank A. Sparks for providing anti-p53 antibodies. ...
doi:10.1074/jbc.274.39.28042
pmid:10488156
fatcat:m7hb3wocszcxljpj5o7qj45z3m
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