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Three-dimensional cell culture technique and pathophysiology

Michiya Matsusaki, Charles Patrick Case, Mitsuru Akashi
2014 Advanced Drug Delivery Reviews  
However, most 3D-cellular constructs are a cell spheroid, which is a heterogeneous aggregation, and thus the reconstruction of the delicate and precise 3D-location of multiple types of cells is almost  ...  In recent years, various novel technologies to develop complex 3D-human tissues including blood and lymph capillary networks have demonstrated that physiological human tissue responses can be replicated  ...  Acknowledgements This work was supported mainly by PRESTO-JST, and partly by an Industrial Technology Research Grant Program in 2006 (06B44017a) from NEDO of Japan, a Grant-in-Aid for Scientific Research  ... 
doi:10.1016/j.addr.2014.01.003 pmid:24462454 fatcat:zq7koqknbbamrkiympjome47dm

Leveling Up Hydrogels: Hybrid Systems in Tissue Engineering

Sara C. Neves, Lorenzo Moroni, Cristina C. Barrias, Pedro L. Granja
2019 Trends in Biotechnology  
Furthermore, we discuss how these systems can contribute to the development of more complex tissue engineered structures in the rapidly evolving field of TERM.  ...  Hydrogels can mimic several features of the cell native microenvironment and have been widely used as synthetic extracellular matrices (ECMs) in tissue engineering and regenerative medicine (TERM).  ...  Acknowledgments This work was supported by Norte Portugal Regional Operational Programme (NORTE2020) under the PORTUGAL 2020 Partnership Agreement through the European Regional Development  ... 
doi:10.1016/j.tibtech.2019.09.004 pmid:31787346 fatcat:ovyhb3sc2bfpfbp2hbwlqbe3lm

Generating vascular conduits: from tissue engineering to three-dimensional bioprinting

Renee M. Maina, Maria J. Barahona, Michele Finotti, Taras Lysyy, Peter Geibel, Francesco D'Amico, David Mulligan, John P. Geibel
2018 Innovative Surgical Science  
We also give an extensive overview of the novel use of 3D bioprinting as means of generating new vascular conduits.  ...  Different strategies have been employed in bioprinting, and the use of cell-based inks to create de novo structures offers a promising solution to bridge the gap of paucity of optimal donor grafts.  ...  This involves assembling 3D cellular structures via magnetic levitation in the presence of paramagnetic agents, such as FDA-approved gadolinium chelates.  ... 
doi:10.1515/iss-2018-0016 pmid:31579784 pmcid:PMC6604577 fatcat:b4n22x3rpbcebl6whjzbyfdp6y

Cell Aggregate Assembly through Microengineering for Functional Tissue Emergence

Gozde Eke, Laurence Vaysse, Xi Yao, Mélanie Escudero, Audrey Carrière, Emmanuelle Trevisiol, Christophe Vieu, Christian Dani, Louis Casteilla, Laurent Malaquin
2022 Cells  
Compared to cell suspensions or monolayers, 3D cell aggregates provide cellular interactions organized in space and heterogeneity that better resume the real organization of native tissues.  ...  In this review, we first present a panorama of emerging cell aggregates microfabrication and assembly technologies.  ...  The magnetic forces are used to maintain the formed 3D architecture while the fusion of the cellular building blocks occurs [29] .  ... 
doi:10.3390/cells11091394 pmid:35563700 pmcid:PMC9102731 fatcat:2545zuwck5ehnewseym7ulqewu

An Efficient Computational Approach to Characterize DSC-MRI Signals Arising from Three-Dimensional Heterogeneous Tissue Structures

Natenael B. Semmineh, Junzhong Xu, Jerrold L. Boxerman, Gary W. Delaney, Paul W. Cleary, John C. Gore, C. Chad Quarles, Karl Herholz
2014 PLoS ONE  
In addition, we illustrate using simulated and micro-CT extracted tissue structures the improved FPFDM along with its potential applications and limitations.  ...  developed in order to efficiently investigate the influence of vascular and extravascular morphological features on susceptibility-induced transverse relaxation.  ...  The two tissue structures were constructed by packing ellipsoids with radii of 5 mm and 15 mm around a fixed fractal-based vascular network.  ... 
doi:10.1371/journal.pone.0084764 pmid:24416281 pmcid:PMC3885618 fatcat:724ohvnszreffpc2sbn6ovmxte

Regulation of the Matrix Microenvironment for Stem Cell Engineering and Regenerative Medicine

Ngan F. Huang, Song Li
2011 Annals of Biomedical Engineering  
The role of ECMs in vascular tissue engineering is then discussed as a model of tissue engineering and regenerative medicine.  ...  The extracellular matrix (ECM) microenvironment consists of structural and functional molecules.  ...  Acknowledgments This work was support in part by research grants from the American Heart Association (to N.H.) and National Institute of Health (HL098688-01A1 to N.H; HL083900 and EB12240 to S.L.)  ... 
doi:10.1007/s10439-011-0297-2 pmid:21424849 pmcid:PMC3568678 fatcat:bxoxvd7snnfk3mzytg55jdqeve

Design of Biomimetic Vascular Grafts with Magnetic Endothelial Patterning

Delphine Fayol, Catherine Le Visage, Julia Ino, Florence Gazeau, Didier Letourneur, Claire Wilhelm
2013 Cell Transplantation  
The combination of imaging modalities [fluorescence imaging, histology, and 3D magnetic resonance imaging (MRI)] evidenced the integrity of the vascular construct.  ...  In particular, the observation of different cell organizations in a vascular scaffold within the range of resolution of single cells by 4.7 T MRI is reported.  ...  The combination of imaging modalities (histology, fluorescence microscopy, and 3D MRI) demonstrated the integrity of the vascular construct for up to 7 days in culture.  ... 
doi:10.3727/096368912x661300 pmid:23295155 fatcat:fvlrynwyibelja75zw5azdg3xy

Multiscale tissue engineering for liver reconstruction

Ryo Sudo
2014 Organogenesis  
Acknowledgments This work was supported by Grants-in-Aid for Scientific Research (25282135, 25560208, 20246041) from the Japan Society for Promotion of Science.  ...  Magnetite nanoparticles have also been used to construct layered 3D structures in vitro; 24 magnetically labeled ECs were seeded onto hepatocyte monolayers cultured under a magnetic field.  ...  Additionally, the size of hepatocyte spheroids is limited by diffusion-dependent mass transfer because of the lack of a vascular network within the 3D structures.  ... 
doi:10.4161/org.27968 pmid:24500493 pmcid:PMC4154956 fatcat:eey2axmnfnetrcjed3d7x4ohoe

Bioprocessing of Tissues using Cellular Spheroids

Timothy R Olsen
2014 Journal of Bioprocessing & Biotechniques  
Recently, magnetic fields have been used as a physical force to accelerate the fusion process with active contacts by increasing cell-cell and cell-matrix interactions in magnetic cellular spheroids [  ...  , or magnetically labeled spheroids can be patterned and aligned using magnetic fields [7, [13] [14] [15] .  ... 
doi:10.4172/2155-9821.1000e112 fatcat:awn7j3azpjbphm42tyqkspsoce

Recent Advancements in 3D Printing and Bioprinting Methods for Cardiovascular Tissue Engineering

Foteini K. Kozaniti, Despoina Nektaria Metsiou, Aikaterini E. Manara, George Athanassiou, Despina D. Deligianni
2021 Bioengineering  
Herein, we sought to encompass the methods of biofabricated scaffolds via 3D printing and bioprinting, the biomaterials and bioinks recruited to create biomimicked tissues of cardiac valves and vascular  ...  networks.  ...  In the Christensen et al. study, vascular-like cellular structures with horizontal and vertical bifurcations were successfully introduced using a liquid support-based inkjet, while the high post-printing  ... 
doi:10.3390/bioengineering8100133 pmid:34677206 fatcat:gaoxzjealjf6vbebe4fywoz7um

Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment

Pei Zhuang, Yi-Hua Chiang, Maria Serafim Fernanda, Mei He
2021 International Journal of Bioprinting  
However, limited control over cellular and structural organization is still the key challenge in achieving in vivo like tissue microenvironment. 3D bioprinting has made great strides toward tissue/organ  ...  We provide a compilation of the contemporary literature sources and summarize the overall advancements in technology and possibilities of using spheroids as building blocks in 3D printed tissue modeling  ...  Acknowledgments The authors would like to thank funding support by NIH NIGMS MIRA award 1R35GM133794 to Dr. Mei He  ... 
doi:10.18063/ijb.v7i4.444 pmid:34805601 pmcid:PMC8600307 fatcat:si2cd2fp3ncdfdahtswxzbvrui

In Vivo Tracking of Tissue Engineered Constructs

Carmen J. Gil, Martin L. Tomov, Andrea S. Theus, Alexander Cetnar, Morteza Mahmoudi, Vahid Serpooshan
2019 Micromachines  
To date, the fields of biomaterials science and tissue engineering have shown great promise in creating bioartificial tissues and organs for use in a variety of regenerative medicine applications.  ...  Fundamental to the further advancement of this field is the design and development of imaging modalities that can enable visualization of the bioengineered constructs following implantation, at adequate  ...  soft tissue structures like hepatic vascular and parenchymal regeneration as well as vascular network at a capillary level.  ... 
doi:10.3390/mi10070474 pmid:31315207 pmcid:PMC6680880 fatcat:by5rnw33xnc3tkwwe3qz3ax4xu

Smart hydrogels for 3D bioprinting

Shuai Wang, Jia Min Lee, Wai Yee Yeong
2015 International Journal of Bioprinting  
Hydrogels are 3D networks that have a high water content.  ...  Among these, "Smart" hydrogels refer to a group of hydrogels that is responsive to various external stimuli such as pH, temperature, light, electric, and magnetic field.  ...  After photo-crosslinking of GelMa, the vascular network was created by removing Pluronic F127 at temperatures below 4℃.  ... 
doi:10.18063/ijb.2015.01.005 fatcat:v3y3pjgzabbjfmtyf2tfu24n3i

Advanced Bottom-Up Engineering of Living Architectures

Vítor M. Gaspar, Pedro Lavrador, João Borges, Mariana B. Oliveira, João F. Mano
2019 Zenodo  
In recent years, this field has seen exciting progress driven by an increasing knowledge of biological systems and their rational deconstruction into key core components.  ...  It is envisioned that a combination of cell–biomaterial constructs with bioadaptable features and biospecific 3D designs will contribute to the development of more robust and functional humanized tissues  ...  Akashi's group has pioneered the research in this field by proposing the build-up of biocompatible tissue constructs comprising L929 fibroblast-based cellular multilayers and native ECM components, including  ... 
doi:10.5281/zenodo.6451076 fatcat:2gejvzrlz5hqfecwgnl6vmajgq

Microscale methods to assemble mammalian cells into tissue-like structures

PeiYuan Gong, Wen Zheng, Dan Xiao, XingYu Jiang
2012 Science China Life Sciences  
They also used this strategy to construct a paracrine signaling network in isolated 3D microtissues.  ...  The magnetic spheroids were patterned using magnetic fields, and then fused together to form a larger tissue construct. Kamihira et al.  ... 
doi:10.1007/s11427-012-4385-9 pmid:23108863 fatcat:4z3xrfcd5ralplotm7i2lts7ve
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