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Multiple response elements and differential p53 binding control Perp expression during apoptosis

Elizabeth E Reczek, Elsa R Flores, Alice S Tsay, Laura D Attardi, Tyler Jacks
2003 Molecular Cancer Research  
Perp is a proapoptotic target gene of p53 expressed to high levels in apoptotic cells compared with those undergoing cell cycle arrest.  ...  Here, we describe the regulation of the Perp gene by p53 through at least three response elements in the Perp promoter and first intron.  ...  Oren for Cyclin G, mdm2, and IGF-BP3 reporters, J. Pietenpol for the HIp53 cell line, K. Macleod for p21 chloramphenicol acetyltransferase reporters, and B.  ... 
pmid:14707288 fatcat:wfijqgrlcfco5iltjpkkxc3kuu

The cell type-specific effect of TAp73 isoforms on the cell cycle and apoptosis

Jitka Holcakova, Pavla Ceskova, Roman Hrstka, Petr Muller, Lenka Dubska, Philip Coates, Emil Palecek, Borivoj Vojtesek
2008 Cellular & Molecular Biology Letters  
Abstractp73, a member of the p53 family, exhibits activities similar to those of p53, including the ability to induce growth arrest and apoptosis. p73 influences chemotherapeutic responses in human cancer  ...  patients, in association with p53.  ...  This study was supported by the grants GAČR 301/05/0416 and GA AV ČR IAA500040513. We especially thank M. Rentoft for the analysis of p63 and p73 status in the used cell lines and M.  ... 
doi:10.2478/s11658-008-0011-z pmid:18350258 fatcat:icbdjazymffdngkiagbe4vc26q

How p53 wields the scales of fate

Amanda M. Goh, David P. Lane
2012 Transcription  
Acknowledgments The authors apologize to those authors whose work could not be cited here due to space constraint.  ...  For example, hematopoietic zinc-finger (HZF) promotes cell cycle arrest over apoptosis by increasing p53 binding to the p21 promoter while decreasing p53 association with the promoters of apoptosis-associated  ...  in cell cycle arrest generally have response elements with a higher affinity for p53 than apoptosis genes. 11 Cell cycle arrest genes thus generally require a lower threshold level of p53 for transcriptional  ... 
doi:10.4161/trns.21297 pmid:22885979 pmcid:PMC3632620 fatcat:ll46zozvrbdufclzsn4zkupjnu

Caveolin-1 Expression Negatively Regulates Cell Cycle Progression by Inducing G0/G1 Arrest via a p53/p21WAF1/Cip1-dependent Mechanism

Ferruccio Galbiati, Daniela Volonte', Jun Liu, Franco Capozza, Philippe G. Frank, Liang Zhu, Richard G. Pestell, Michael P. Lisanti, Carl-Henrik Heldin
2001 Molecular Biology of the Cell  
We show that serum starvation induces up-regulation of endogenous caveolin-1 and arrests cells in the G 0 /G 1 phase of the cell cycle.  ...  Analysis of primary cultures of mouse embryonic fibroblasts from caveolin-1 transgenic mice reveals that caveolin-1 induces 1) cells to exit the S phase of the cell cycle with a concomitant increase in  ...  ACKNOWLEDGMENTS This work was supported by grants from the National Institutes of Health, the Muscular Dystrophy Association, the American Heart Association, and the Susan B.  ... 
doi:10.1091/mbc.12.8.2229 pmid:11514613 pmcid:PMC58591 fatcat:gr2sqqivljhbtl754n56rmf4sm

PRAP1 is a novel executor of p53-dependent mechanisms in cell survival after DNA damage

B H Huang, J L Zhuo, C H W Leung, G D Lu, J J Liu, C T Yap, S C Hooi
2012 Cell Death and Disease  
PRAP1 appears to protect cells from apoptosis by inducing cell-cycle arrest, suggesting that the induction of PRAP1 expression by p53 in response to DNA-damaging agents contributes to cancer cell survival  ...  We identified functional p53-response elements within intron 1 of PRAP1 gene and showed that these regions interact directly with p53 using ChIP assays, indicating that PRAP1 is a novel p53 target gene  ...  The sequences of these two p53-response elements in PRAP1 matched the consensus p53 binding site by 84% and 70%, while the known p53-response element in the p21 promoter showed a match of 90% (Table 1  ... 
doi:10.1038/cddis.2012.180 pmid:23235459 pmcid:PMC3542616 fatcat:ukf3gaxahvc6jbad3p3vrnufa4

P53 in blind subterranean mole rats – loss-of-function versus gain-of-function activities on newly cloned Spalax target genes

A Avivi, O Ashur-Fabian, A Joel, L Trakhtenbrot, K Adamsky, I Goldstein, N Amariglio, G Rechavi, E Nevo
2006 Oncogene  
This substitution reduces the p53 effect on the transcription of apoptosis genes (apaf1, puma, pten and noxa) and enhances it on human cell cycle arrest and p53 stabilization/homeostasis genes (mdm2, pten  ...  We compared the Spalax-responsive elements to those of human, mouse and rat and investigated the transcriptional activity of Spalax and human Arg174Lys-mutated p53 on target genes of both species.  ...  Acknowledgements We appreciate Dr Imad Shams help in this study, and we thank Eti Rosenthal for her technical assistance with the cell cycle experiments.  ... 
doi:10.1038/sj.onc.1210045 pmid:17043642 fatcat:3qb6une6czhadmgwpujhise4lm

Sox2+ cells in Sonic Hedgehog-subtype medulloblastoma resist p53-mediated cell-cycle arrest response and drive therapy-induced recurrence

Daniel M Treisman, Yinghua Li, Brianna R Pierce, Chaoyang Li, Andrew P Chervenak, Gerald J Tomasek, Guillermina Lozano, Xiaoyan Zheng, Marcel Kool, Yuan Zhu
2019 Neuro-Oncology Advances  
Conclusion Quiescent Sox2+ cells are efficiently eliminated by p53-mediated apoptosis, but not cell-cycle arrest and differentiation.  ...  The same treatment eliminated most Sox2- bulk tumor cells in SHH-MBs harboring p53R172P, an apoptosis-defective allele with cell-cycle arrest activity, via inducing robust neuronal differentiation.  ...  tumor suppressive responses, including apoptosis, cell-cycle arrest, and senescence. 15 Consistently, genetic studies using mouse models have demonstrated that disruption of p53-mediated apoptosis confers  ... 
doi:10.1093/noajnl/vdz027 pmid:31763624 pmcid:PMC6860004 fatcat:w4ok3muatbfsdhbjvdz47v5co4

Of flies and men; p53, a tumour suppressor

Josephine E. Sutcliffe, Alexander Brehm
2004 FEBS Letters  
The suitability of the fruit fly to study the function of the tumour suppressor p53 is further exemplified by the lack of p53 family members within the fly genome, i.e., no homologues to p63 and p73 have  ...  Hence, there is no redundancy between family members greatly facilitating the analysis of p53 function.  ...  Acknowledgements: We apologize to colleagues whose work we could not cite because of space limitations. Work in the Brehm Laboratory is supported by the DFG.  ... 
doi:10.1016/j.febslet.2004.03.122 pmid:15165898 fatcat:lmttkshqkjehnjqnri7dajz534

Insights into Selective Activation of p53 DNA Binding by c-Abl

Gang Wei, Andrew G. Li, Xuan Liu
2005 Journal of Biological Chemistry  
As a transcription factor, p53 recognizes a specific consensus DNA sequence and activates the expression of the target genes involved in either growth arrest or apoptosis.  ...  The implications of this with relation to selective activation of p53 target genes involved in either growth arrest or apoptosis are discussed.  ...  Nie for assistance with baculovirus protein expression and purification. We thank all members of our laboratory, especially L. G.  ... 
doi:10.1074/jbc.m409522200 pmid:15661746 fatcat:6jarbg6t7fe5zpznopwnkfut3q

Knockin mice expressing a chimeric p53 protein reveal mechanistic differences in how p53 triggers apoptosis and senescence

T. M. Johnson, K. Meade, N. Pathak, M. R. Marques, L. D. Attardi
2008 Proceedings of the National Academy of Sciences of the United States of America  
This chimeric mutant, termed p53 VP16 , robustly activates the transcription of a range of p53 targets involved in both apoptosis and senescence.  ...  The contribution of transcriptional activation to the p53 effector functions critical for tumor suppression, apoptosis and cellular senescence, remains unclear because of p53's ability to regulate diverse  ...  Tuveson for the transcriptional stop cassette, and S. Lowe and E. DeStanchina for the anti-Pml antibody. We also thank S. Artandi and J. Sage for their critical reading of the manuscript.  ... 
doi:10.1073/pnas.0706764105 pmid:18216268 pmcid:PMC2234118 fatcat:jvxp5uunfvgmxlsk5d63zspuh4

Transcriptional Regulation during p21WAF1/CIP1-induced Apoptosis in Human Ovarian Cancer Cells

Qun Wu, Paul Kirschmeier, Tish Hockenberry, Tong-Yuan Yang, Diana L. Brassard, Luquan Wang, Terri McClanahan, Stuart Black, Giovanni Rizzi, Mary Lynn Musco, Asra Mirza, Suxing Liu
2002 Journal of Biological Chemistry  
1 The abbreviations used are: rAd-p53, adenovirus expressing p53; rAd-p21, adenovirus expressing p21 WAF1⁄CIP1 ; rAd-empty vector, adenovirus containing an empty vector; PCNA, proliferating cell nuclear  ...  antigen; RT, reverse transcription; FACS, fluorescence-activated cell sorter; TUNEL, terminal dUTP nick-end labeling.  ...  When cancer cells experience cell cycle arrest and/or apoptosis in response to p53, the induced p21 WAF1⁄CIP1 mRNA and the cell proliferation-related genes are down-regulated.  ... 
doi:10.1074/jbc.m204962200 pmid:12138103 fatcat:aihe2fafgvetbdbxjbdzlt77oy

p53 oligomerization status modulates cell fate decisions between growth, arrest and apoptosis

Nicholas W. Fischer, Aaron Prodeus, David Malkin, Jean Gariépy
2016 Cell Cycle  
We report that dimeric p53 variants are cytostatic and can arrest cell growth, but lack the ability to trigger apoptosis in p53-null cells.  ...  Activation of p53 through tetramerization is required for its tumor suppressive function by inducing transcriptional programs that lead to cell fate decisions such as cell cycle arrest or apoptosis.  ...  Nadeem Moghal for the generous use of equipment for qRT-PCR experiments and Dr. Cheryl Arrowsmith for the kind gift of the p53 variant MQLR.  ... 
doi:10.1080/15384101.2016.1241917 pmid:27754743 pmcid:PMC5176156 fatcat:dnbecvuosbhonbwoakkbhxpce4

p53 directly transactivates Δ133p53α, regulating cell fate outcome in response to DNA damage

M Aoubala, F Murray-Zmijewski, M P Khoury, K Fernandes, S Perrier, H Bernard, A-C Prats, D P Lane, J-C Bourdon
2010 Cell Death and Differentiation  
The name of the transcription factors, position and sequence of the responsive element are also indicated.  ...  Human ∆133p53α antagonizes p53-mediated apoptosis and G1 cell cycle arrest without preventing G2 cell cycle arrest.  ... 
doi:10.1038/cdd.2010.91 pmid:20689555 pmcid:PMC3039296 fatcat:gwurcjwu5bgerlwrvkjv572wx4

Stimulus-Specific Transcriptional Regulation Within the p53 Network

Aaron Joseph Donner, Jennifer Michelle Hoover, Stephanie Aspen Szostek, Joaquín M. Espinosa
2007 Cell Cycle  
an anti-apoptotic role, as the lack of p53 results in less cell cycle arrest and increased apoptosis. 17 Finally, Nutlin-3 induces a cell cycle arrest response with cells accumulating in both G 1 and  ...  In colorectal cancer cells, UVC triggers apoptosis, doxorubicin induces transient cell cycle arrest followed by apoptosis, and Nutlin-3 leads to cell cycle arrest with no significant apoptosis.  ... 
doi:10.4161/cc.6.21.4893 pmid:17957141 pmcid:PMC2927486 fatcat:6sovrt7k4nbxjleazzthgmgsni

p53-dependent repression of polo-like kinase-1 (PLK1)

Lynsey McKenzie, Sharon King, Lynnette Marcar, Sam Nicol, Sylvia S. Dias, Katie Schumm, Pamela Robertson, Jean-Christophe Bourdon, Neil Perkins, Frances Fuller-Pace, David Meek
2010 Cell Cycle  
Knock-down of PLK1 in the HCT116 (p53 +/+ ) cells ( fig. 9a ) led to cell cycle arrest at both the G 1 and G 2 /M phases of the cycle with a small proportion of the cells undergoing apoptosis as evidenced  ...  [1] [2] [3] [4] p53 is induced in response to a variety of cellular stresses including DNA damage, leading mainly to the onset of apoptosis or cell cycle arrest at the G 1 /S and G 2 /M boundaries.  ...  The sequence of these regions is given in figure 5a.  ... 
doi:10.4161/cc.9.20.13532 pmid:20962589 pmcid:PMC3055203 fatcat:euopvwxoz5da7lnedlwiq7nlku
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