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Hyperdimensional Imaging Contrast Using an Optical Fiber

Jenu V. Chacko, Han Nim Lee, Wenxin Wu, Marisa S. Otegui, Kevin W. Eliceiri
2021 Sensors  
Fluorescence lifetime imaging microscopy (FLIM) with controlled excitation polarization and temporally dispersed emission can generate a spectrally coded, polarization-filtered lifetime distribution for  ...  Here we present a fiber-based imaging system that can generate hyper-dimensional contrast by combining multiple fluorescence properties into a single fluorescence lifetime decay curve.  ...  Future work in these directions to optimize HD-Contrast Imaging are underway, and we hope this can become an unsupervised biomedical tool for fluorescence contrast.  ... 
doi:10.3390/s21041201 pmid:33572130 fatcat:g6w2cuccybewboj4jgs5tkn7vi

Imaging Intracellular Signaling Using Two-Photon Fluorescent Lifetime Imaging Microscopy

R. Yasuda
2012 Cold Spring Harbor Protocols  
In particular, fluorescence lifetime imaging in combination with two-photon laser-scanning microscopy (two-photon fluorescence lifetime imaging microscopy [2pFLIM]) is a powerful tool to monitor signaling  ...  This article provides practical guidelines for quantitative imaging of intracellular signaling using 2pFLIM.  ...  One way to visualize fluorescence lifetime images is to code the fluorescence lifetime with color and to code fluorescence intensity with brightness ) (Fig. 2) .  ... 
doi:10.1101/pdb.top072090 pmid:23118363 fatcat:4xvld6jkmbb5ld7fxqce7x3vtq

Time-Domain Fluorescence Lifetime Imaging Techniques Suitable for Solid-State Imaging Sensor Arrays

David Day-Uei Li, Simon Ameer-Beg, Jochen Arlt, David Tyndall, Richard Walker, Daniel R. Matthews, Viput Visitkul, Justin Richardson, Robert K. Henderson
2012 Sensors  
2012) Time-domain fluorescence lifetime imaging techniques suitable for solid-state imaging sensor arrays. Sensors, 12 (5).  ...  Keywords: fluorescence lifetime imaging microscopy; FLIM; CMOS; single-photon avalanche diode; single-decay; multi-decay; time-domain FLIM; lifetime based sensing Introduction Fluorescence based imaging  ...  Figure 4 . 4 (a) (Video) Movie of CMM lifetime codes of fluorescent beads in a micro-channel at a video frame rate of 50 fps and (b) a single frame of the estimated lifetime video.  ... 
doi:10.3390/s120505650 pmid:22778606 pmcid:PMC3386705 fatcat:lfrupk454fc2znaanydmelqcoy

Single-laser shot fluorescence lifetime imaging on the nanosecond timescale using a Dual Image and Modeling Evaluation algorithm

Andreas Ehn, Olof Johansson, Andreas Arvidsson, Marcus Aldén, Joakim Bood
2012 Optics Express  
In combination with the DIME detector scheme, it is a faster, more accurate and more sensitive approach for rapid fluorescence lifetime imaging than previously proposed techniques.  ...  A novel technique, designated dual imaging and modeling evaluation (DIME), for evaluating single-laser shot fluorescence lifetimes is presented.  ...  Acknowledgments This work has been financed by the Swedish Energy Agency, SSF (Swedish Foundation for Strategic Research) through CECOST (Centre for Combustion Science and Technology) and DALDECS, an Advanced  ... 
doi:10.1364/oe.20.003043 pmid:22330541 fatcat:k6yi5qcs2vgvhkyo6ahc7bznqi

A Fast Global Fitting Algorithm for Fluorescence Lifetime Imaging Microscopy Based on Image Segmentation

S. Pelet, M.J.R. Previte, L.H. Laiho, P.T. C. So
2004 Biophysical Journal  
Global fitting algorithms have been shown to improve effectively the accuracy and precision of the analysis of fluorescence lifetime imaging microscopy data.  ...  This method further allows second harmonic generation from collagen and elastin autofluorescence to be differentiated in fluorescence lifetime imaging microscopy images of ex vivo human skin.  ...  The authors acknowledge the support of the National Institutes for Health grant NIHPOIHL64858.  ... 
doi:10.1529/biophysj.104.045492 pmid:15454472 pmcid:PMC1304699 fatcat:qfc5mkbymzdjfmjepiip6o5fz4

Improving Diagnosis of Cervical Pre-Cancer: Combination of PCA and SVM Applied on Fluorescence Lifetime Images

Gyana Sahoo, Pankaj Singh, Kiran Pandey, Chayanika Kala, Asima Pradhan
2018 Photonics  
The fast and slow components of double exponential fitted fluorescence lifetimes were found to be higher for EH compared to the lifetimes of CIN-I samples.  ...  decay profile of Fluorescence Lifetime Images (FLI) of epithelial hyperplasia (EH) and CIN-I cervical tissue samples, obtained ex-vivo.  ...  Gyana Ranjan Sahoo would like to thank Prabodh Kumar Pandey for technical help. Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/photonics5040057 fatcat:hsmvjs6kubb6tel6akizoyksme

Squaraine-Based Polymer Dots with Narrow, Bright Near-Infrared Fluorescence for Biological Applications

I-Che Wu, Jiangbo Yu, Fangmao Ye, Yu Rong, Maria Elena Gallina, Bryant S. Fujimoto, Yong Zhang, Yang-Hsiang Chan, Wei Sun, Xing-Hua Zhou, Changfeng Wu, Daniel T. Chiu
2014 Journal of the American Chemical Society  
Fluorescent copolymers containing fluorene and squaraine units were synthesized and used as precursors for preparing the Pdots, where exciton diffusion and likely through-bond energy transfer led to highly  ...  We demonstrate bioconjugation of the squaraine Pdots and employ the Pdot bioconjugates in flow cytometry and cellular imaging applications.  ...  ■ ACKNOWLEDGMENTS We gratefully acknowledge support for this research from the National Institutes of Health (R21CA186798) and the University of Washington.  ... 
doi:10.1021/ja5123045 pmid:25494172 pmcid:PMC4304448 fatcat:bnalxu5g7zay7cjokknhkcaor4

Nanosecond-order long–short fluorescence lifetime switchable encryption with enlarged coding capacity

Teng Luo, Yihua Zhao, Ting Zhou, Junle Qu
2021 Nanophotonics  
The turn-off fluorescent photoswitches for information encryption are constantly being developed.  ...  average fluorescence lifetime (τi), which will realize the supermultiplexed fluorescence lifetime switchable encryption.  ...  For the subsequent optimization of fluorescence lifetime multiplexing for different eosin solvents, we changed the lifetime color-coded range of the alphabets "Biophotonics Group" in Figure 2D2 (τm)  ... 
doi:10.1515/nanoph-2021-0054 fatcat:wdfpg3vasbhjzgxrli3xu7qh2a

Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging

Joseph R Pyle, Jixin Chen
2017 Beilstein Journal of Nanotechnology  
Many YOYO-1 molecules intercalate into DNA and remain there during imaging, and most of them have to be temporarily or permanently fluorescently bleached, often stochastically, to allow for the visualization  ...  Super-resolution imaging of single DNA molecules via point accumulation for imaging in nanoscale topography (PAINT) has great potential to visualize fine DNA structures with nanometer resolution.  ...  Katherine Cimatu, Kurt Sy Piecco, and Juvinch Vicente for beneficial discussions.  ... 
doi:10.3762/bjnano.8.229 pmid:29181286 pmcid:PMC5687005 fatcat:zfo6ptazgvgh7hg6rb5sbhnsji

Single-shot time-gated fluorescence lifetime imaging using three-frame images

Yahui Li, Hui Jia, Shaorong Chen, Jinshou Tian, Lingliang Liang, Fenfang Yuan, Hongqi Yu, David Day-Uei Li
2018 Optics Express  
Abstract Qualitative and quantitative measurements of complex flows demand for fast single-shot fluorescence lifetime imaging (FLI) technology with high precision.  ...  OCIS codes: (110.4155) Multiframe image processing; (170.3650) Lifetime-based sensing; (170.6920) Time-resolved imaging; (280.2490) Flow diagnostics; (300.2530) Fluorescence, laser-induced.  ...  TFI-TGFLI optimizes in the ranges, < 2.7ns for F and < 1.3ns for FA, whereas ORLD optimizes in a longer range.  ... 
doi:10.1364/oe.26.017936 pmid:30114076 fatcat:byg4c2ksrjfxvlb7kisgktq3i4

Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems

Md Abdul Kader Sagar, Bing Dai, Jenu V. Chacko, Joshua J. Weber, Andreas Velten, Scott T. Sanders, John G. White, Kevin W. Eliceiri
2019 Journal of Biomedical Optics  
We present a fiber-based method to obtain spectral information from a multiphoton fluorescence lifetime imaging (FLIM) system.  ...  However, it is difficult to obtain both channels of information in a conventional scheme as detectors are typically configured either for spectral or lifetime detection.  ...  Acknowledgments We acknowledge funding from the Morgridge Institute for Research, the Laboratory for Optical and Computational Instrumentation, U.S.  ... 
doi:10.1117/1.jbo.25.1.014506 pmid:31833280 pmcid:PMC6907392 fatcat:gssxq3eup5gk5jykhel7yo7sgu

Hyperspectral time-resolved wide-field fluorescence molecular tomography based on structured light and single-pixel detection

Qi Pian, Ruoyang Yao, Lingling Zhao, Xavier Intes
2015 Optics Letters  
We present a time-resolved fluorescence diffuse optical tomography platform that is based on wide-field structured illumination, single-pixel detection, and hyperspectral acquisition.  ...  The main system characteristics are reported, and we demonstrate the feasibility of acquiring dense 4D tomographic data sets (space, time, spectra) for time domain 3D quantitative multiplexed fluorophore  ...  The authors gratefully acknowledge the technical support of the Computational Center for Nanotechnology Innovations (CCNI) at RPI.  ... 
doi:10.1364/ol.40.000431 pmid:25680065 pmcid:PMC4638422 fatcat:cl3o7zqvpnhftjqfyc5wwq67p4

Tunable lifetime nanocrystals

Renren Deng, Xiaogang Liu
2014 Nature Photonics  
Coupled with recent advances in fluorescence lifetime imaging microscopy 9 , the experimental capabilities presented here may provide optical multiplexing solutions for living specimens with extremely  ...  This will facilitate data interpretation, help optimize the resolution and aid the development of new microscopy schemes.  ... 
doi:10.1038/nphoton.2013.353 fatcat:k4sptktsfvg2pf3izebrk2rski

On-the-fly decoding luminescence lifetimes in the microsecond region for lanthanide-encoded suspension arrays

Yiqing Lu, Jie Lu, Jiangbo Zhao, Janet Cusido, Françisco M Raymo, Jingli Yuan, Sean Yang, Robert C. Leif, Yujing Huo, James A. Piper, J Paul Robinson, Ewa M. Goldys (+1 others)
2014 Nature Communications  
Here we show a fast algorithm suitable for the microsecond region with precision closely approaching the theoretical limit and compatible with the rapid scanning cytometry technique.  ...  Significant multiplexing capacity of optical time-domain coding has been recently demonstrated by tuning luminescence lifetimes of the upconversion nanoparticles called 't-Dots'.  ...  Y.L. and D.J. acknowledge the International Society for Advancement of Cytometry for support as ISAC Scholars.  ... 
doi:10.1038/ncomms4741 pmid:24796249 pmcid:PMC4024748 fatcat:gryopb7pcngt7m3v3jcd4o7x4q

Optical Estimation of Absolute Membrane Potential Using One- and Two-Photon Fluorescence Lifetime Imaging Microscopy [article]

Julia R. Lazzari-Dean, Evan W. Miller
2021 bioRxiv   pre-print
To study Vmem in these diverse contexts, it is essential to accurately record absolute values of Vmem, rather than solely relative measurements.Materials & MethodsWe use fluorescence lifetime imaging of  ...  demonstrate that VF-FLIM is a robust and portable metric for Vmem across imaging platforms and under both one-photon and two-photon illumination.  ...  We thank Holly Aaron and Feather Ives for expert technical assistance.  ... 
doi:10.1101/2021.02.16.431491 fatcat:dibnc4ygirfmtctlq4pumwe6i4
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