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Classification of the dermal-epidermal junction using in-vivo confocal microscopy
2017
2017 IEEE 14th International Symposium on Biomedical Imaging (ISBI 2017)
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We improve the restitution of the epidermal and dermal labels while reducing the thickness of the uncertainty area in a coherent biological way from 16.9 µm (ground-truth) to 10.3 µm. ...
Reflectance confocal microscopy (RCM) is a powerful tool to visualize the skin layers at cellular resolution. The dermalepidermal junction (DEJ) is a thin complex 3D structure. ...
In this paper, we provide a method to automatically segment the dermo-epidermal junction in 3 classes : epidermis (ED), uncertain area (U), and dermis (D) using in vivo reflectance confocal microscopy ...
doi:10.1109/isbi.2017.7950513
dblp:conf/isbi/RobicPNCT17
fatcat:f7zo64t74fbqbnznjukpev2qvi
Reflectance confocal microscopy characteristics for melanocytic nevi
2019
Chinese Medical Journal
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It will probably further increase in vivo diagnostic acumen and provide new insights into the in vivo dynamics of melanocytic neoplasms. ...
Reflectance confocal microscopy analysis had revealed ringed or meshwork structures in majority of junctional nevi. ...
Conflicts of interest None. ...
doi:10.1097/cm9.0000000000000440
pmid:31567389
pmcid:PMC6831067
fatcat:w3bzmjqlbbcyfevjd5qi3c7j44
Confocal Microscopy in Diagnosis and Management of Melasma: Review of Literature
2015
Journal of Pigmentary Disorders
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Reflectance Confocal Microscopy (RCM) is a novel, non-invasive technique that offers an in vivo analysis of the skin layers at a cellular level resolution. ...
of brighter cellular structures at the level of the dermo-epidermal junction. ...
RCM findings were in accordance with previous studies, confirming the usefulness of in vivo confocal analysis in the evaluation of melasma treatment [26] (Figures 1 and 2 ). ...
doi:10.4172/2376-0427.1000s1005
fatcat:xijbsgelfffrldobdywtmw7c3q
A novel biomarker harvesting nanotechnology identifies Bak as a candidate melanoma biomarker in serum
2010
Experimental Dermatology
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Biomarker levels were correlated with confocal microscopy, thereby representing a combination of new technologies for in vivo histologic documentation. ...
Conclusions-Our study links serum proteome analysis with confocal microscopic clinical in vivo histologic classification of melanomas. Bak has not been previously measured in serum. ...
Acknowledgments This work was partly supported by the Italian Istituto Superiore di Sanità, Rome, Italy in the framework of the Italy/ USA cooperation agreement between the U.S. ...
doi:10.1111/j.1600-0625.2010.01187.x
pmid:21158936
pmcid:PMC3777620
fatcat:pxceygzdyzbq5i24qyq6x2v43e
In vivoreflectance-mode confocal microscopy assessments: impact of overweight on human skin microcirculation and histomorphology
2016
Journal of Biomedical Optics
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Reflectance-mode confocal microscopy (RCM) enables in vivo assessment of the human skin. ...
evaluated using RCM: dermal blood cell flow (DBCF), density of dermal capillaries (DDC), epidermal thickness (ET), and epidermal cell size (ECS). ...
Fig. 4 4 Fig. 4 Illustrated is the ET measured in vivo using confocal microscopy as the distance between skin surface and the apical plane of the dermal papillae. ...
doi:10.1117/1.jbo.21.3.036009
pmid:27020601
fatcat:lkezsrp4hvca3g4ks6wzyujcqe
Automated Segmentation of Skin Strata in Reflectance Confocal Microscopy Depth Stacks
2016
PLoS ONE
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Reflectance confocal microscopy (RCM) is a powerful tool for in-vivo examination of a variety of skin diseases. ...
The mean absolute error in determining the interface depth for each of the stratum corneum/viable epidermis, viable epidermis/dermal-epidermal junction and dermal-epidermal junction/papillary dermis interfaces ...
Introduction Reflectance confocal microscopy (RCM) is capable of imaging human skin in-vivo at high resolution, with available machines capable of lateral resolutions of 0.5-1 μ m [1] . ...
doi:10.1371/journal.pone.0153208
pmid:27088865
pmcid:PMC4835045
fatcat:yq6sdfl2ave7phy7tbjbh3skpi
Automated segmentation of skin strata in reflectance confocal microscopy depth stacks
[article]
2015
bioRxiv
pre-print
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Reflectance confocal microscopy (RCM) is a powerful tool for in-vivo examination of a variety of skin diseases. ...
The mean absolute error in determining the interface depth for each of the stratum corneum/viable epidermis, viable epidermis/dermal-epidermal junction and dermal-epidermal junction/papillary dermis interfaces ...
Introduction Reflectance confocal microscopy (RCM) is capable of imaging human skin in-vivo at high resolution, with available machines capable of lateral resolutions of 0.5-1 μ m [1] . ...
doi:10.1101/022137
fatcat:uq6ivoiofzbvtn4tfkf6jgfiiu
Histometric data obtained by in vivo confocal laser scanning microscopy in patients with systemic sclerosis
2002
BMC Dermatology
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To investigate the skin of patients with systemic sclerosis using confocal laser scanning microscopy in vivo and to develop histometric parameters to describe characteristic cutaneous changes of systemic ...
All subjects were investigated using confocal laser scanning microscopy in vivo. ...
In vivo confocal laser scanning microscopy (CLSM) A commercially available CLSM (Vivascope 1000; Lucid Inc, Henrietta, NY; [http://www.lucid-tech.com] was used for in vivo confocal laser scanning microscopy ...
doi:10.1186/1471-5945-2-8
pmid:12162791
pmcid:PMC122067
fatcat:osy6cxhmnzer7fgfovrkee5diy
Reflectance confocal microscopy features of BRAF V600E mutated thin melanomas detected by immunohistochemistry
2017
PLoS ONE
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BRAF positive melanomas were related to younger age (p = 0.035), invasive melanomas (p = 0.03) and to the presence of hiporreflective cells (p = 0.02), epidermal nests (p = 0.02), dermal-epidermal junction ...
We analyzed BRAF V600E mutated melanomas to explore the Reflectance confocal microscopy (RCM) utility as a screening aid in the evaluation of the most appropriate patients for genetic testing. ...
Reflectance confocal microscopy and immunohistochemistry of BRAF V600E in thin melanomas One of the novel findings of our study was that not only the presence of dermal-epidermal nest but also the presence ...
doi:10.1371/journal.pone.0179745
pmid:28662062
pmcid:PMC5491027
fatcat:ztkvcxdo6ncuplgwqydp52y4wu
In Vivo Microscopic Features of Nodular Melanomas
2008
Archives of Dermatology
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Objective: To characterize nodular melanoma (NM) using dermoscopy, in vivo reflectance-mode confocal microscopy, and histopathologic analysis. ...
Design: Consecutive pure NMs and superficial spreading melanomas (SSMs) with nodular or blue areas were studied using dermoscopy and confocal microscopy, and a correlation with histopathologic findings ...
The nonvisibility of dermal papillae resulted at confocal microscopy in the sudden transition between epidermal layers and dermal structures, corresponding at histologic analysis with a thin flattened ...
doi:10.1001/archderm.144.10.1311
pmid:18936395
fatcat:dqswtgarpranzjwy77syi3yrxu
Abstracts from the 2016 Joint Meeting of the International Confocal Group (ICG), the International Dermoscopy Society (IDS), and the International Society for Digital Imaging of the Skin (ISDIS)
2016
Dermatology Practical & Conceptual
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Acquired confocal images clearly visualized characteristic cellular features of human skin down to the dermal-epidermal junction, including cell nuclei in spinous layer and dermal papilla. ...
We present preliminary results of imaging human skin in vivo with slit-SECM. ...
Acquired confocal images clearly visualized characteristic cellular features of human skin down to the dermal-epidermal junction, including cell nuclei in spinous layer and dermal papilla. ...
doi:10.5826/dpc.0602a11
fatcat:3kt5cdcquvhj3awhnh7rutowp4
Artificial Intelligence-Based Approaches to Reflectance Confocal Microscopy Image Analysis in Dermatology
2022
Journal of Clinical Medicine
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However, the current visual method for identifying the dermal-epidermal junction (DEJ) in RCM images is subjective, and there is a lot of variation. ...
Given that in vivo RCM is becoming more widely used in dermatology, numerous deep learning technologies have been developed in recent years to provide a more objective approach to RCM image analysis. ...
Conflicts of Interest: The authors declare no conflict of interest. ...
doi:10.3390/jcm11020429
pmid:35054123
pmcid:PMC8780225
fatcat:uh735ndpdbanleivh72t35srfq
Handheld In Vivo Reflectance Confocal Microscopy for the Diagnosis of Eyelid Margin and Conjunctival Tumors
2017
JAMA ophthalmology
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OBJECTIVE To assess the diagnostic accuracy of handheld in vivo reflectance confocal microscopy (IVCM) for the diagnosis of eyelid margin and conjunctival tumors. ...
Analysis of all neoformations (155 excised and 140 in follow-up) confirmed these differences in the diagnostic accuracy of the clinical examination and IVCM. ...
In vivo reflectance confocal microscopy diagnosis was in agreement with the histopathologic examination in all 7 cases of MM, showing sensitivity of 100% for MM, but it misdiagnosed MM in 2 dermal nevi ...
doi:10.1001/jamaophthalmol.2017.2019
pmid:28654937
pmcid:PMC5710297
fatcat:ii6u5vok5zhaxgfiai73t7dfdi
Automating reflectance confocal microscopy image analysis for dermatological research: a review
2022
Journal of Biomedical Optics
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Algorithms for image classification to epidermal layers, delineation of the dermal-epidermal junction, classification of skin lesions, and demarcation of individual cells within an image, all important ...
Reflectance confocal microscopy (RCM) is a noninvasive, in vivo technology that offers near histopathological resolution at the cellular level. ...
RCM, reflectance confocal microscopy; DEJ, dermal-epidermal junction. ...
doi:10.1117/1.jbo.27.7.070902
pmid:35879817
pmcid:PMC9309100
fatcat:llvma322vjfxpkoutu6tzboyly
Reflectance-mode confocal microscopy of pigmented skin lesions–improvement in melanoma diagnostic specificity
2005
Journal of American Academy of Dermatology
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In vivo confocal microscopy enables skin visualization with a quasihistopathologic resolution. ...
In multivariate analysis, the presence of nonedged dermal papillae, atypical cells, and isolated nucleated cells within dermal papilla, pagetoid cells, widespread pagetoid infiltration, and cerebriform ...
II Frequency of in vivo reflectance-mode confocal laser microscopy features and odds ratio values observed at basal layers and dermoepidermal junction in benign and malignant melanocytic lesions MM (37 ...
doi:10.1016/j.jaad.2005.08.022
pmid:16310058
fatcat:uvsaulrhk5hhfbdma543hp57l4
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