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However, the challenges of reconstruction and quantification of alternatively spliced full-length circRNAs remain unresolved. ... Circular RNAs (circRNAs), covalently closed continuous RNA loops, are generated from cognate linear RNAs through back splicing events, and alternative splicing events may generate different circRNA isoforms ... Therefore, to address the problem of full-length assembly and quantification of circRNA, in this work, we developed CircAST. ...doi:10.1016/j.gpb.2019.03.004 pmid:32007626 pmcid:PMC7056934 fatcat:jnm34xu4qnanbbuixj7t5rsqvm
Compared to the assumed circular isoforms derived from linear transcript annotations, some of the alternatively spliced circular isoforms have 100 times higher expression and contain substantially fewer ... Here we describe psirc, a method that can identify full-length circRNA isoforms and quantify their expression levels from RNA sequencing data. ... splice site selection-namely, cassette exon, intron retention, alternative 5 ′ splice site, and alternative 3 ′ splice site-can all be found in circRNAs (Zhang et al. 2016) . ...doi:10.1101/gr.275348.121 pmid:34663689 pmcid:PMC8647826 fatcat:ieuhn75cvfauzaqd33ptw33lhm
, and studying alternative splicing structure. ... involved in RNA sequencing, analysis, and software development. ... Patro for his valuable comments and suggestions on the manuscript. Competing Interests The authors declare no competing interests. References ...arXiv:2010.02391v3 fatcat:krgm7uzmibdlfh2ylgqxmnh2qu
The FEBS Journal
Circular RNAs (circRNAs) are a recently discovered class of non-coding RNAs found in many species across the eukaryotic kingdom. ... These intriguing RNA species are formed through a unique mechanism that is known as back splicing in which the 5' and 3' termini are covalently joined. ... Ea Chee Kwee (University of Texas Southwestern Medical Center) and Dr. Jennifer Ann Harikrishna (Universiti Malaya) for critical review of the manuscript. ...doi:10.1111/febs.15639 pmid:33236482 fatcat:43bs5kgn6nag7fy35swsj4dnaq