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Mild cognitive impairment (MCI) defines an intermediate state between normal ageing and dementia, including Alzheimer's disease (AD). Identification of MCI subjects who will progress to AD (MCI-AD) is today of crucial importance, especially in light of the possible development of new pathogenic therapies. Several evidences suggest that miRNAs could play relevant roles in the biogenesis of AD, and the links between selected miRNAs and specific pathogenic aspects have been partly explored. Indoi:10.1007/s12035-020-02029-7 pmid:32737762 fatcat:w35obstlbnbbnnkhzavjztkkn4
more »... study, we analysed the composition of microRNA transcriptome in blood, serum and cerebrospinal fluid samples from MCI-AD subjects, from an enriched small RNA library. Real-time qPCR from MCI-AD and AD patients and normal controls was performed to profile miRNA expression. In particular, four microRNAs, hsa-mir-5588-5p, hsa-mir-3658, hsa-mir-567 and hsa-mir-3908, among all selected microRNAs, are dysregulated. Hsa-mir-567 was found to be differentially expressed in cerebrospinal fluid samples, blood and serum from MCI-AD patients, showing the highest fold change and statistical significance. Target prediction analysis have been performed to evaluate mRNAs whose expression was controlled by miRNAs found to be dysregulated here, showing that hsa-mir-567 target genes are functionally active in neuronal cells. We propose that miRNA profiles found in samples from MCI-AD patients might be relevant for a better understanding of AD-related cognitive decline and could lead to set up suitable and potential biomarkers for MCI-AD progression to AD.
Barbara Brunetto (a) , Sonia Brescianini (b) , Bianca Barletta (c) , Cinzia Butteroni (c) , Daniela Rotondi (b) , Rosalba Masciulli (d) , Malaguti Aliberti (d) , Carlo Pini (a) , Gabriella Di Felice (c ... Centro Nazionale per la Ricerca e la Valutazione dei Prodotti Immunobiologici; (b) Centro Nazionale di Epidemiologia, Sorveglianza e Promozione della Salute; (c) Dipartimento di Malattie Infettive, ...pmid:20061662 fatcat:7ualen2dmzalfdmvsyoxj7gtku
Label-free single-molecule detection has been achieved so far by funnelling a large number of ligands into a sequence of single-binding events with few recognition elements host on nanometric transducers. Such approaches are inherently unable to sense a cue in a bulk milieu. Conceptualizing cells' ability to sense at the physical limit by means of highly-packed recognition elements, a millimetric sized field-effect-transistor is used to detect a single molecule. To this end, the gate isdoi:10.1038/s41467-018-05235-z pmid:30104563 pmcid:PMC6089965 fatcat:jemzz2ttujgqpmk2sxts7h2kx4
more »... tionalized with a self-assembled-monolayer of 10 12 capturing anti-Immunoglobulin-G and is endowed with a hydrogen-bonding network enabling cooperative interactions. The selective and label-free single molecule IgG detection is strikingly demonstrated in diluted saliva while 15 IgGs are assayed in whole serum. The suggested sensing mechanism, triggered by the affinity binding event, involves a work-function change that is assumed to propagate in the gating-field through the electrostatic hydrogen-bonding network. The proposed immunoassay platform is general and can revolutionize the current approach to protein detection.
Parasites & Vectors
Experimental BALB/c model Infection and immunization Forty-nine eight-week old female BALB/c mice (20 ± 2 g) were housed in the Animal Care Unit of the Istituto Superiore di Sanità (ISS), Rome, Italy. ... worm extract; CWE: Crude worm extract; ELISA: Enzyme immunosorbent assay; FBS: Fetal bovine sera; i.p.: Intraperitoneally; IFN: Interferon; Ig: Immunoglobulin; IL: Interleukin; ISS: Istituto Superiore di ... Ethics approval The Animal Care Committee of the Istituto Superiore di Sanità approved the in vivo protocol n°8/2014-B of January 15th 2014 from the Ministry of Health (DL 116/92). ...doi:10.1186/s13071-017-2231-4 pmid:28606183 pmcid:PMC5468942 fatcat:biv43eoer5aoxaunbkkak4ple4
Normalized SiMoT EG-FET responses (ΔI/I0)/(DI/I0) sat with (-DI/I0) sat = 0.74 ± 0.13. ... Normalized SiMoT EG-FET responses, (ΔI/I 0 )/(DI/I 0 ) sat with (-DI/I 0 ) sat = 0.74 ± 0.13 for the data taken in 100 µl and (-DI/I 0 ) sat = 0.47 ± 0.14 for the data measured in 1 ml. ... Supporting Information Why a diffusing single-molecule can be detected in few minutes by a large capturing bioelectronic interface Eleonora Macchia, Liberato De Caro, Fabrizio Torricelli, Cinzia Di Franco ...arXiv:2202.00949v1 fatcat:wlux4x6w2na3focsxfpx33gkoy
(1) Background: Ozone (O3) proved to oxidize organic and inorganic compounds, and its efficacy against bacteria, viruses and fungi plasma membranes was of interest. Ozone vehicle can be a gaseous form, ozonated water or ozonized oil. The aim of this in-vitro study was to evaluate the efficacy of ozonated olive oil against Streptococcus mutans. (2) Methods: Two different commercial mouthwashes were tested: Ialozon Blu (IB) (Gemavip, Cagliari, Italy), with ozonated olive oil, and Ialozon Rosedoi:10.3390/ijerph17239106 pmid:33291253 fatcat:iwifuawh2nfw3iu4j4xpix7sj4
more »... (Gemavip, Cagliari, Italy), with ozonated olive oil, hyaluronic acid and vitamin E. All formulates were analyzed in a dilution range from 2- to 256-folds in saline solution, as to reproduce the salivary dilution. Streptococcus mutans CIP103220 strain was used for the antimicrobial susceptibility test, and the Kirby–Bauer inhibition method was performed to evaluate the Minimum Inhibitory (MIC), Minimum Bactericidal (MBC), and Minimum Biofilm Inhibitory Concentration (MBIC). (3) Results: Both formulates showed the same antimicrobial activity. MIC, MBC, and MBIC were observed for dilution factors of 1/32, 1/8 and 1/8, respectively. The mean value of inhibition zone diameter was 16.5 mm for IB, and 18 mm for IR. (4) Conclusions: The results suggested that ozonized olive oil formulates were able to inactivate Streptococcus mutans avoiding the salivary dilution effect in the oral cavity.
IntroductionEndonasal endoscopic surgery has changed the treatment perspectives for different lesions of the hypothalamic–pituitary region. The metastases of the hypothalamic–pituitary region represent 0.4% of all intracranial metastatic tumors and account for only 1.8% of surgically managed pituitary lesions. The aim of tshis study is to describe a single-center institutional experience with 13 cases of hypothalamic–pituitary metastasis focused on presurgical workup, the evolution of thedoi:10.3389/fonc.2022.975738 pmid:35965539 pmcid:PMC9366217 fatcat:wq4irumgpzbutewidhpt53wcfi
more »... al technique, and postsurgical management according to our protocols, showing effects on progression-free and overall survival rates for this relatively uncommon location.Material and MethodsWe retrospectively reviewed the whole series of patients that received the endoscopic endonasal approach at the Division of Neurosurgery at the University of Naples "Federico II" undergoing surgery from January 1997 to December 2021. We identified 13 cases whose pathology reports revealed a metastatic lesion. Statistical analysis was performed to determine the Kaplan–Meier survival function and assess for log-rank differences in survival based on gender, surgical treatment, and postoperative therapy (p-value < 0.02*).ResultsThe pathology report disclosed lung adenocarcinoma (six cases, 46%), breast adenocarcinoma (two cases, 15.4%), clear cell renal carcinoma (one case, 7%), melanoma (one case, 7%), colorectal adenocarcinoma (one case, 7%), uterine cervix carcinoma (one case, 7%), and follicular thyroid carcinoma (one case, 7%). A standard endoscopic endonasal approach was performed in 10 patients (76.9%), while an extended endonasal procedure was performed in only three cases (23%). Biopsy was the surgical choice in five patients with infiltrative and invasive lesions and a poor performance status (38%), while in the cases where neurovascular decompression was necessary, a subtotal resection was achieved in five patients (38%) and partial resection in three patients (23%). Recovery of visual field defect was observed in six of seven patients with visual loss (85.7%), improvement of oculomotor nerve palsy occurred in four of seven patients with this defect (57.1%), while the impairment of oculomotor palsy was observed in three patients (42.9%). Visual function was stable in the other patients. The median progression-free survival and overall survival were 14 and 18 months, respectively. There were statistically significant differences in PFS and OS in patients who underwent adjuvant radiotherapy (p=0.019 is referred to OS and p=0.017 to PFS, respectively; p-value = 0.02).ConclusionsThe endoscopic endonasal approach is a viable approach for the management of hypothalamic–pituitary metastases as this surgery provides an adequate opportunity to obtain tissue sample and neurovascular decompression, both being crucial for continuing the integrated adjuvant therapy protocols.
Worldwide population ageing is partly due to advanced standard of care, leading to increased incidence and prevalence of geriatric syndromes such as frailty and disability. Hence, the age at the onset of acute coronary syndromes (ACS) keeps growing as well. Moreover, ageing is a risk factor for both frailty and cardiovascular disease (CVD). Frailty and CVD in the elderly share pathophysiological mechanisms and associated conditions, such as malnutrition, sarcopenia, anemia, polypharmacy anddoi:10.3390/jcm10081696 pmid:33920796 fatcat:ldgvkjq575goxodydu6idslnw4
more »... increased bleeding/thrombotic risk, leading to a negative impact on outcomes. In geriatric populations ACS is associated with an increased frailty degree that has a negative effect on re-hospitalization and mortality outcomes. Frail elderly patients are increasingly referred to cardiac rehabilitation (CR) programs after ACS; however, plans of care must be tailored on individual's clinical complexity in terms of functional capacity, nutritional status and comorbidities, cognitive status, socio-economic support. Completing rehabilitative intervention with a reduced frailty degree, disability prevention, improvement in functional state and quality of life and reduction of re-hospitalization are the goals of CR program. Tools for detecting frailty and guidelines for management of frail elderly patients post-ACS are still debated. This review focused on the need of an early identification of frail patients in elderly with ACS and at elaborating personalized plans of care and secondary prevention in CR setting.
Butteroni, Silvia Corinti, Gabriella Di Felice From Food Allergy and Anaphylaxis Meeting 2011 Venice, Italy. 17-19 February 2011 © 2011 Schiavi et al; licensee BioMed Central Ltd. ... Probiotic treatment induces intestinal regulatory dendritic and T cells, and counter-regulates Th2 responses and anaphylaxis in a mouse model of food allergy Elisa Schiavi * , Bianca Barletta, Andrea Barone, Cinzia ...doi:10.1186/2045-7022-1-s1-o30 pmcid:PMC3354088 fatcat:57zrm2nzhndo7eyvby5u7pil5a
Despite the rising evidence in favor of immunotherapy (IT), the treatment of oncological patients affected by so-called "cold tumors" still represents an open issue. Cold tumors are characterized by an immunosuppressive (so-called cold) tumor microenvironment (TME), which favors host immune system suppression, cancer immune-escape, and a worse response to IT. However, the TME is not a static element, but dynamically mutates and can be changed. Radiotherapy (RT) can modulate a colddoi:10.3390/curroncol29070366 pmid:35877226 pmcid:PMC9319790 fatcat:wvsw7j6udfhtdfpyxoyszyhbja
more »... nt, rendering it better at tumor killing by priming the quiescent host immune system, with a consequent increase in immunotherapy response. The combination of TME radiomodulation and IT could therefore be a strategy for those patients affected by cold tumors, with limited or no response to IT. Thus, this review aims to provide an easy, rapid, and practical overview of how RT could convert the cold TME and why cold tumor radiomodulation could represent an interesting strategy in combination with IT.
length of follow-up time available in the case of positive FIT results was insufficient.The discrepancy between the number eligible and the number invited in each round is accounted for by subjects who died ...doi:10.1016/j.cgh.2020.08.038 pmid:32827723 fatcat:mnn6dgrd5febvj26e3yyokuqd4
They observed that high lactate production was a common expected feature of all neoplastic cells, as the most discriminative features between different cell clusters were various di-and tripeptides (valine-leucine ...doi:10.3390/ijms23084173 pmid:35456991 pmcid:PMC9030452 fatcat:lcwynxipwjac3dqwexosmnsuv4
Calcium-binding plant allergens can be grouped in different families according to the number of calciumbinding domains (EF hands). Objective: We sought to identify pollens containing crossreactive calcium-binding allergens and to investigate structural and immunologic similarities of members belonging to different families of calcium-binding allergens. Methods: By means of multiple sequence alignment and molecular modeling, we searched for structural similarities among pollen allergens with 2doi:10.1067/mai.2002.121528 pmid:11842303 fatcat:rr5yvcvrsffo7dpfb5suiia4l4
more »... hl p 7, timothy grass; Aln g 4, alder), (Bet v 3, birch) and 4 EF hands (Jun o 4, prickly juniper). Purified recombinant Aln g 4 and Jun o 4 were used to determine the prevalence of IgE recognition in 210 patients sensitized to different pollens and to search, by means of ELISA competition, for the presence of cross-reactive epitopes in pollens from 16 unrelated plant species. IgE cross-reactivity among the allergen families was studied with purified rPhl p 7, rAln g 4, rBet v 3, and rJun o 4 and 2 synthetic peptides comprising the N-terminal and C-terminal EF hands of Phl p 7 by means of ELISA competition. Results: Structural similarities were found by using molecular modeling among the allergens with 2, 3, and 4 EF hands. Pollens from 16 unrelated plants contained Aln g 4-and Jun o 4-related epitopes. Twenty-two percent of the patients with multiple pollen sensitization reacted to at least one of the calcium-binding allergens. A hierarchy of IgE cross-reactivity (rPhl p7 > rAln g 4 > rJun o 4 > rBet v 3) could be established that identified rPhl p 7 as the EF-hand allergen containing most IgE epitopes in the population studied. Conclusion: The demonstration that members of different families of calcium-binding plant allergens share similarities suggests that it may be possible to use representative molecules for the diagnosis and therapy of allergies to EF-hand allergens. (J Allergy Clin Immunol 2002;109:314-20.)
Controls were conducted as untreated negative controls in natural filtered seawater (FSW) collected offshore from "Stazione Zoologica di Napoli A. ... Some phthalates such as DEHP (Di(2-ethylhexyl) phthalate) and MEHP (Mono(2ethylhexyl) phthalate) and their metabolites were found in amniotic fluid because of the maternal exposure  . ...doi:10.1155/2010/251767 pmid:20706694 pmcid:PMC2913803 fatcat:4ung4nmli5egvf3qvtw6pd4qzq
Acknowledgments The membership of the Emilia-Romagna Region Workgroup for Cervical Screening Evaluation is as follows: Nicoletta Bertozzi, Enza Di Felice, Stefano Ferretti, Alba Carola Finarelli, Carlo ...doi:10.1002/ijc.31806 pmid:30120770 fatcat:tfbp276t2beixp5rzwn34lohjq
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