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MicroRNA Expression Signature in Mild Cognitive Impairment Due to Alzheimer's Disease

Bruna De Felice, Concetta Montanino, Mariano Oliva, Simona Bonavita, Valeria Di Onofrio, Cinzia Coppola
2020 Molecular Neurobiology  
Mild cognitive impairment (MCI) defines an intermediate state between normal ageing and dementia, including Alzheimer's disease (AD). Identification of MCI subjects who will progress to AD (MCI-AD) is today of crucial importance, especially in light of the possible development of new pathogenic therapies. Several evidences suggest that miRNAs could play relevant roles in the biogenesis of AD, and the links between selected miRNAs and specific pathogenic aspects have been partly explored. In
more » ... study, we analysed the composition of microRNA transcriptome in blood, serum and cerebrospinal fluid samples from MCI-AD subjects, from an enriched small RNA library. Real-time qPCR from MCI-AD and AD patients and normal controls was performed to profile miRNA expression. In particular, four microRNAs, hsa-mir-5588-5p, hsa-mir-3658, hsa-mir-567 and hsa-mir-3908, among all selected microRNAs, are dysregulated. Hsa-mir-567 was found to be differentially expressed in cerebrospinal fluid samples, blood and serum from MCI-AD patients, showing the highest fold change and statistical significance. Target prediction analysis have been performed to evaluate mRNAs whose expression was controlled by miRNAs found to be dysregulated here, showing that hsa-mir-567 target genes are functionally active in neuronal cells. We propose that miRNA profiles found in samples from MCI-AD patients might be relevant for a better understanding of AD-related cognitive decline and could lead to set up suitable and potential biomarkers for MCI-AD progression to AD.
doi:10.1007/s12035-020-02029-7 pmid:32737762 fatcat:w35obstlbnbbnnkhzavjztkkn4

Exposure to indoor allergens and association with allergy symptoms of employees in a work environment

Barbara Brunetto, Sonia Brescianini, Bianca Barletta, Cinzia Butteroni, Daniela Rotondi, Rosalba Masciulli, Malaguti Aliberti, Carlo Pini, Gabriella Di Felice, Patrizia Iacovacci
2009 Annali dell'Istituto Superiore di Sanità  
Barbara Brunetto (a) , Sonia Brescianini (b) , Bianca Barletta (c) , Cinzia Butteroni (c) , Daniela Rotondi (b) , Rosalba Masciulli (d) , Malaguti Aliberti (d) , Carlo Pini (a) , Gabriella Di Felice (c  ...  Centro Nazionale per la Ricerca e la Valutazione dei Prodotti Immunobiologici; (b) Centro Nazionale di Epidemiologia, Sorveglianza e Promozione della Salute; (c) Dipartimento di Malattie Infettive,  ... 
pmid:20061662 fatcat:7ualen2dmzalfdmvsyoxj7gtku

Single-molecule detection with a millimetre-sized transistor

Eleonora Macchia, Kyriaki Manoli, Brigitte Holzer, Cinzia Di Franco, Matteo Ghittorelli, Fabrizio Torricelli, Domenico Alberga, Giuseppe Felice Mangiatordi, Gerardo Palazzo, Gaetano Scamarcio, Luisa Torsi
2018 Nature Communications  
Label-free single-molecule detection has been achieved so far by funnelling a large number of ligands into a sequence of single-binding events with few recognition elements host on nanometric transducers. Such approaches are inherently unable to sense a cue in a bulk milieu. Conceptualizing cells' ability to sense at the physical limit by means of highly-packed recognition elements, a millimetric sized field-effect-transistor is used to detect a single molecule. To this end, the gate is
more » ... tionalized with a self-assembled-monolayer of 10 12 capturing anti-Immunoglobulin-G and is endowed with a hydrogen-bonding network enabling cooperative interactions. The selective and label-free single molecule IgG detection is strikingly demonstrated in diluted saliva while 15 IgGs are assayed in whole serum. The suggested sensing mechanism, triggered by the affinity binding event, involves a work-function change that is assumed to propagate in the gating-field through the electrostatic hydrogen-bonding network. The proposed immunoassay platform is general and can revolutionize the current approach to protein detection.
doi:10.1038/s41467-018-05235-z pmid:30104563 pmcid:PMC6089965 fatcat:jemzz2ttujgqpmk2sxts7h2kx4

Allergenic activity of Pseudoterranova decipiens (Nematoda: Anisakidae) in BALB/c mice

Alessandra Ludovisi, Gabriella Di Felice, Noelia Carballeda-Sangiao, Bianca Barletta, Cinzia Butteroni, Silvia Corinti, Gianluca Marucci, Miguel González-Muñoz, Edoardo Pozio, Maria Angeles Gómez-Morales
2017 Parasites & Vectors  
Experimental BALB/c model Infection and immunization Forty-nine eight-week old female BALB/c mice (20 ± 2 g) were housed in the Animal Care Unit of the Istituto Superiore di Sanità (ISS), Rome, Italy.  ...  worm extract; CWE: Crude worm extract; ELISA: Enzyme immunosorbent assay; FBS: Fetal bovine sera; i.p.: Intraperitoneally; IFN: Interferon; Ig: Immunoglobulin; IL: Interleukin; ISS: Istituto Superiore di  ...  Ethics approval The Animal Care Committee of the Istituto Superiore di Sanità approved the in vivo protocol n°8/2014-B of January 15th 2014 from the Ministry of Health (DL 116/92).  ... 
doi:10.1186/s13071-017-2231-4 pmid:28606183 pmcid:PMC5468942 fatcat:biv43eoer5aoxaunbkkak4ple4

Why a diffusing single-molecule can be detected in few minutes by a large capturing bioelectronic interface [article]

Eleonora Macchia, Liberato De Caro, Fabrizio Torricelli, Cinzia Di Franco, Giuseppe Felice Mangiatordi, Gaetano Scamarcio, Luisa Torsi
2022 arXiv   pre-print
Normalized SiMoT EG-FET responses (ΔI/I0)/(DI/I0) sat with (-DI/I0) sat = 0.74 ± 0.13.  ...  Normalized SiMoT EG-FET responses, (ΔI/I 0 )/(DI/I 0 ) sat with (-DI/I 0 ) sat = 0.74 ± 0.13 for the data taken in 100 µl and (-DI/I 0 ) sat = 0.47 ± 0.14 for the data measured in 1 ml.  ...  Supporting Information Why a diffusing single-molecule can be detected in few minutes by a large capturing bioelectronic interface Eleonora Macchia, Liberato De Caro, Fabrizio Torricelli, Cinzia Di Franco  ... 
arXiv:2202.00949v1 fatcat:wlux4x6w2na3focsxfpx33gkoy

Mouthwash Based on Ozonated Olive Oil in Caries Prevention: A Preliminary In-Vitro Study

Gianna Maria Nardi, Sara Fais, Cinzia Casu, Marta Mazur, Roberto Di Giorgio, Roberta Grassi, Felice Roberto Grassi, Germano Orrù
2020 International Journal of Environmental Research and Public Health  
(1) Background: Ozone (O3) proved to oxidize organic and inorganic compounds, and its efficacy against bacteria, viruses and fungi plasma membranes was of interest. Ozone vehicle can be a gaseous form, ozonated water or ozonized oil. The aim of this in-vitro study was to evaluate the efficacy of ozonated olive oil against Streptococcus mutans. (2) Methods: Two different commercial mouthwashes were tested: Ialozon Blu (IB) (Gemavip, Cagliari, Italy), with ozonated olive oil, and Ialozon Rose
more » ... (Gemavip, Cagliari, Italy), with ozonated olive oil, hyaluronic acid and vitamin E. All formulates were analyzed in a dilution range from 2- to 256-folds in saline solution, as to reproduce the salivary dilution. Streptococcus mutans CIP103220 strain was used for the antimicrobial susceptibility test, and the Kirby–Bauer inhibition method was performed to evaluate the Minimum Inhibitory (MIC), Minimum Bactericidal (MBC), and Minimum Biofilm Inhibitory Concentration (MBIC). (3) Results: Both formulates showed the same antimicrobial activity. MIC, MBC, and MBIC were observed for dilution factors of 1/32, 1/8 and 1/8, respectively. The mean value of inhibition zone diameter was 16.5 mm for IB, and 18 mm for IR. (4) Conclusions: The results suggested that ozonized olive oil formulates were able to inactivate Streptococcus mutans avoiding the salivary dilution effect in the oral cavity.
doi:10.3390/ijerph17239106 pmid:33291253 fatcat:iwifuawh2nfw3iu4j4xpix7sj4

Evolution in endoscopic endonasal approach for the management of hypothalamic–pituitary region metastasis: A single-institution experience

Cinzia Baiano, Teresa Somma, Raduan Ahmed Franca, Marianna Di Costanzo, Maria Rosaria Scala, Pasquale Cretella, Felice Esposito, Luigi Maria Cavallo, Paolo Cappabianca, Domenico Solari
2022 Frontiers in Oncology  
IntroductionEndonasal endoscopic surgery has changed the treatment perspectives for different lesions of the hypothalamic–pituitary region. The metastases of the hypothalamic–pituitary region represent 0.4% of all intracranial metastatic tumors and account for only 1.8% of surgically managed pituitary lesions. The aim of tshis study is to describe a single-center institutional experience with 13 cases of hypothalamic–pituitary metastasis focused on presurgical workup, the evolution of the
more » ... al technique, and postsurgical management according to our protocols, showing effects on progression-free and overall survival rates for this relatively uncommon location.Material and MethodsWe retrospectively reviewed the whole series of patients that received the endoscopic endonasal approach at the Division of Neurosurgery at the University of Naples "Federico II" undergoing surgery from January 1997 to December 2021. We identified 13 cases whose pathology reports revealed a metastatic lesion. Statistical analysis was performed to determine the Kaplan–Meier survival function and assess for log-rank differences in survival based on gender, surgical treatment, and postoperative therapy (p-value < 0.02*).ResultsThe pathology report disclosed lung adenocarcinoma (six cases, 46%), breast adenocarcinoma (two cases, 15.4%), clear cell renal carcinoma (one case, 7%), melanoma (one case, 7%), colorectal adenocarcinoma (one case, 7%), uterine cervix carcinoma (one case, 7%), and follicular thyroid carcinoma (one case, 7%). A standard endoscopic endonasal approach was performed in 10 patients (76.9%), while an extended endonasal procedure was performed in only three cases (23%). Biopsy was the surgical choice in five patients with infiltrative and invasive lesions and a poor performance status (38%), while in the cases where neurovascular decompression was necessary, a subtotal resection was achieved in five patients (38%) and partial resection in three patients (23%). Recovery of visual field defect was observed in six of seven patients with visual loss (85.7%), improvement of oculomotor nerve palsy occurred in four of seven patients with this defect (57.1%), while the impairment of oculomotor palsy was observed in three patients (42.9%). Visual function was stable in the other patients. The median progression-free survival and overall survival were 14 and 18 months, respectively. There were statistically significant differences in PFS and OS in patients who underwent adjuvant radiotherapy (p=0.019 is referred to OS and p=0.017 to PFS, respectively; p-value = 0.02).ConclusionsThe endoscopic endonasal approach is a viable approach for the management of hypothalamic–pituitary metastases as this surgery provides an adequate opportunity to obtain tissue sample and neurovascular decompression, both being crucial for continuing the integrated adjuvant therapy protocols.
doi:10.3389/fonc.2022.975738 pmid:35965539 pmcid:PMC9366217 fatcat:wq4irumgpzbutewidhpt53wcfi

Frailty in Acute and Chronic Coronary Syndrome Patients Entering Cardiac Rehabilitation

Francesco Giallauria, Anna Di Lorenzo, Elio Venturini, Mario Pacileo, Antonello D'Andrea, Umberto Garofalo, Felice De Lucia, Crescenzo Testa, Gianluigi Cuomo, Gabriella Iannuzzo, Marco Gentile, Cinzia Nugara (+4 others)
2021 Journal of Clinical Medicine  
Worldwide population ageing is partly due to advanced standard of care, leading to increased incidence and prevalence of geriatric syndromes such as frailty and disability. Hence, the age at the onset of acute coronary syndromes (ACS) keeps growing as well. Moreover, ageing is a risk factor for both frailty and cardiovascular disease (CVD). Frailty and CVD in the elderly share pathophysiological mechanisms and associated conditions, such as malnutrition, sarcopenia, anemia, polypharmacy and
more » ... increased bleeding/thrombotic risk, leading to a negative impact on outcomes. In geriatric populations ACS is associated with an increased frailty degree that has a negative effect on re-hospitalization and mortality outcomes. Frail elderly patients are increasingly referred to cardiac rehabilitation (CR) programs after ACS; however, plans of care must be tailored on individual's clinical complexity in terms of functional capacity, nutritional status and comorbidities, cognitive status, socio-economic support. Completing rehabilitative intervention with a reduced frailty degree, disability prevention, improvement in functional state and quality of life and reduction of re-hospitalization are the goals of CR program. Tools for detecting frailty and guidelines for management of frail elderly patients post-ACS are still debated. This review focused on the need of an early identification of frail patients in elderly with ACS and at elaborating personalized plans of care and secondary prevention in CR setting.
doi:10.3390/jcm10081696 pmid:33920796 fatcat:ldgvkjq575goxodydu6idslnw4

Probiotic treatment induces intestinal regulatory dendritic and T cells, and counter-regulates Th2 responses and anaphylaxis in a mouse model of food allergy

Elisa Schiavi, Bianca Barletta, Andrea Barone, Cinzia Butteroni, Silvia Corinti, Gabriella Di Felice
2011 Clinical and Translational Allergy  
Butteroni, Silvia Corinti, Gabriella Di Felice From Food Allergy and Anaphylaxis Meeting 2011 Venice, Italy. 17-19 February 2011 © 2011 Schiavi et al; licensee BioMed Central Ltd.  ...  Probiotic treatment induces intestinal regulatory dendritic and T cells, and counter-regulates Th2 responses and anaphylaxis in a mouse model of food allergy Elisa Schiavi * , Bianca Barletta, Andrea Barone, Cinzia  ... 
doi:10.1186/2045-7022-1-s1-o30 pmcid:PMC3354088 fatcat:57zrm2nzhndo7eyvby5u7pil5a

Can Radiotherapy Empower the Host Immune System to Counterattack Neoplastic Cells? A Systematic Review on Tumor Microenvironment Radiomodulation

Federico Iori, Alessio Bruni, Salvatore Cozzi, Patrizia Ciammella, Francesca Di Pressa, Luca Boldrini, Carlo Greco, Valerio Nardone, Viola Salvestrini, Isacco Desideri, Francesca De Felice, Cinzia Iotti
2022 Current Oncology  
Despite the rising evidence in favor of immunotherapy (IT), the treatment of oncological patients affected by so-called "cold tumors" still represents an open issue. Cold tumors are characterized by an immunosuppressive (so-called cold) tumor microenvironment (TME), which favors host immune system suppression, cancer immune-escape, and a worse response to IT. However, the TME is not a static element, but dynamically mutates and can be changed. Radiotherapy (RT) can modulate a cold
more » ... nt, rendering it better at tumor killing by priming the quiescent host immune system, with a consequent increase in immunotherapy response. The combination of TME radiomodulation and IT could therefore be a strategy for those patients affected by cold tumors, with limited or no response to IT. Thus, this review aims to provide an easy, rapid, and practical overview of how RT could convert the cold TME and why cold tumor radiomodulation could represent an interesting strategy in combination with IT.
doi:10.3390/curroncol29070366 pmid:35877226 pmcid:PMC9319790 fatcat:wvsw7j6udfhtdfpyxoyszyhbja

Results of Compliant Participation in 5 Rounds of Fecal Immunochemical Test Screening for Colorectal Cancer

Flavia Baldacchini, Lauro Bucchi, Orietta Giuliani, Silvia Mancini, Alessandra Ravaioli, Rosa Vattiato, Paolo Giorgi Rossi, Cinzia Campari, Debora Canuti, Enza Di Felice, Francesca Mezzetti, Priscilla Sassoli de Bianchi (+2 others)
2020 Clinical Gastroenterology and Hepatology  
length of follow-up time available in the case of positive FIT results was insufficient.The discrepancy between the number eligible and the number invited in each round is accounted for by subjects who died  ... 
doi:10.1016/j.cgh.2020.08.038 pmid:32827723 fatcat:mnn6dgrd5febvj26e3yyokuqd4

Metabolomic Approaches for Detection and Identification of Biomarkers and Altered Pathways in Bladder Cancer

Nicola Antonio di Meo, Davide Loizzo, Savio Domenico Pandolfo, Riccardo Autorino, Matteo Ferro, Camillo Porta, Alessandro Stella, Cinzia Bizzoca, Leonardo Vincenti, Felice Crocetto, Octavian Sabin Tataru, Monica Rutigliano (+3 others)
2022 International Journal of Molecular Sciences  
They observed that high lactate production was a common expected feature of all neoplastic cells, as the most discriminative features between different cell clusters were various di-and tripeptides (valine-leucine  ... 
doi:10.3390/ijms23084173 pmid:35456991 pmcid:PMC9030452 fatcat:lcwynxipwjac3dqwexosmnsuv4

Molecular, structural, and immunologic relationships between different families of recombinant calcium-binding pollen allergens

Raffaella Tinghino, Anna Twardosz, Bianca Barletta, Eleonora M.R. Puggioni, Patrizia Iacovacci, Cinzia Butteroni, Claudia Afferni, Adriano Mari, Brigitte Hayek, Gabriella Di Felice, Margarete Focke, Kerstin Westritschnig (+2 others)
2002 Journal of Allergy and Clinical Immunology  
Calcium-binding plant allergens can be grouped in different families according to the number of calciumbinding domains (EF hands). Objective: We sought to identify pollens containing crossreactive calcium-binding allergens and to investigate structural and immunologic similarities of members belonging to different families of calcium-binding allergens. Methods: By means of multiple sequence alignment and molecular modeling, we searched for structural similarities among pollen allergens with 2
more » ... hl p 7, timothy grass; Aln g 4, alder), (Bet v 3, birch) and 4 EF hands (Jun o 4, prickly juniper). Purified recombinant Aln g 4 and Jun o 4 were used to determine the prevalence of IgE recognition in 210 patients sensitized to different pollens and to search, by means of ELISA competition, for the presence of cross-reactive epitopes in pollens from 16 unrelated plant species. IgE cross-reactivity among the allergen families was studied with purified rPhl p 7, rAln g 4, rBet v 3, and rJun o 4 and 2 synthetic peptides comprising the N-terminal and C-terminal EF hands of Phl p 7 by means of ELISA competition. Results: Structural similarities were found by using molecular modeling among the allergens with 2, 3, and 4 EF hands. Pollens from 16 unrelated plants contained Aln g 4-and Jun o 4-related epitopes. Twenty-two percent of the patients with multiple pollen sensitization reacted to at least one of the calcium-binding allergens. A hierarchy of IgE cross-reactivity (rPhl p7 > rAln g 4 > rJun o 4 > rBet v 3) could be established that identified rPhl p 7 as the EF-hand allergen containing most IgE epitopes in the population studied. Conclusion: The demonstration that members of different families of calcium-binding plant allergens share similarities suggests that it may be possible to use representative molecules for the diagnosis and therapy of allergies to EF-hand allergens. (J Allergy Clin Immunol 2002;109:314-20.)
doi:10.1067/mai.2002.121528 pmid:11842303 fatcat:rr5yvcvrsffo7dpfb5suiia4l4

Assessment of DNA Damage by RAPD inParacentrotus lividusEmbryos Exposed to Amniotic Fluid from Residents Living Close to Waste Landfill Sites

Maurizio Guida, Marco Guida, Bruna De Felice, Daniela Santafede, Raffaella D'Alessandro, Attilio Di Spiezio Sardo, Marianna Scognamiglio, Cinzia Ferrara, Giuseppe Bifulco, Carmine Nappi
2010 Journal of Biomedicine and Biotechnology  
Controls were conducted as untreated negative controls in natural filtered seawater (FSW) collected offshore from "Stazione Zoologica di Napoli A.  ...  Some phthalates such as DEHP (Di(2-ethylhexyl) phthalate) and MEHP (Mono(2ethylhexyl) phthalate) and their metabolites were found in amniotic fluid because of the maternal exposure [17] .  ... 
doi:10.1155/2010/251767 pmid:20706694 pmcid:PMC2913803 fatcat:4ung4nmli5egvf3qvtw6pd4qzq

Estimating the impact of an organised screening programme on cervical cancer incidence: a 26-year study from northern Italy

Lauro Bucchi, Flavia Baldacchini, Silvia Mancini, Alessandra Ravaioli, Orietta Giuliani, Rosa Vattiato, Fabio Falcini, Paolo Giorgi Rossi, Cinzia Campari, Debora Canuti, Enza Di Felice, Priscilla Sassoli de Bianchi (+3 others)
2018 International Journal of Cancer  
Acknowledgments The membership of the Emilia-Romagna Region Workgroup for Cervical Screening Evaluation is as follows: Nicoletta Bertozzi, Enza Di Felice, Stefano Ferretti, Alba Carola Finarelli, Carlo  ... 
doi:10.1002/ijc.31806 pmid:30120770 fatcat:tfbp276t2beixp5rzwn34lohjq
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