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A generic selection system for improved expression and thermostability of G protein-coupled receptors by directed evolution

Christoph Klenk, Janosch Ehrenmann, Marco Schütz, Andreas Plückthun
2016 Scientific Reports  
With more than 800 members, G protein-coupled receptors (GPCRs) represent the largest superfamily of IMPs and are considered as one of the most important drug targets 1,2 .  ...  Structural and biophysical studies as well as drug screening approaches on G protein-coupled receptors (GPCRs) have been largely hampered by the poor biophysical properties and low expression yields of  ...  Cells were transformed with the respective plasmids by electroporation and were grown at 30 °C in 2× YT supplemented with 10 mg/mL glucose and 100 μ g/mL ampicillin.  ... 
doi:10.1038/srep21294 pmid:26887595 pmcid:PMC4758055 fatcat:nnf5eh2kenen3nythbobm75aoy

Performance of Automated Drusen Detection by Polarization-Sensitive Optical Coherence Tomography

Ferdinand G. Schlanitz, Bernhard Baumann, Tobias Spalek, Christopher Schütze, Christian Ahlers, Michael Pircher, Erich Götzinger, Christoph K. Hitzenberger, Ursula Schmidt-Erfurth
2011 Investigative Ophthalmology and Visual Science  
(F) Feature of the thickness map of the depolarizing layer, (G) the corresponding near-infrared autofluorescence (NIR-AF) image, both with arrows indicating the drusen indicated (D) respectively (E).  ... 
doi:10.1167/iovs.10-6846 pmid:21474772 fatcat:h4g62mu5c5aldfc7cruscw3j4e

Pathogen-Reactive T Helper Cell Analysis in the Pig

Friederike Ebner, Patrycja Schwiertz, Svenja Steinfelder, Robert Pieper, Jürgen Zentek, Nicole Schütze, Christoph G. Baums, Gottfried Alber, Peter Geldhof, Susanne Hartmann
2017 Frontiers in Immunology  
ml Streptomycin (PAN-Biotech), Gentamycin (50 µg/ml, PAN-Biotech), and Amphotericin B (0.25 µg/ml, PAN-Biotech)].  ...  lysate (40 µg/ml, GREER ® ) for 6 h and in presence of BrefeldinA (3 µg/ml, eBioscience) during the last 4 h of restimulation.  ... 
doi:10.3389/fimmu.2017.00565 pmid:28567041 pmcid:PMC5434156 fatcat:xgkf7ndvgrfjnidan2yeiwy4qy

Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts

Marco Schütz, Jendrik Schöppe, Erik Sedlák, Matthias Hillenbrand, Gabriela Nagy-Davidescu, Janosch Ehrenmann, Christoph Klenk, Pascal Egloff, Lutz Kummer, Andreas Plückthun
2016 Scientific Reports  
a G protein-independent fashion 2 .  ...  With about 800 different members in humans, G protein-coupled receptors (GPCRs) are the largest superfamily of cell surface receptors 1 .  ...  G protein (Gα i 1 β 1 γ 1 ) was expressed in Sf9 cells using a single baculovirus encoding all three subunits.  ... 
doi:10.1038/srep21508 pmid:26911446 pmcid:PMC4766470 fatcat:ubqvhqxr3rftflflzmxlea54we

Performance of Drusen Detection by Spectral-Domain Optical Coherence Tomography

Ferdinand G. Schlanitz, Christian Ahlers, Stefan Sacu, Christopher Schütze, Marcos Rodriguez, Sabine Schriefl, Isabelle Golbaz, Tobias Spalek, Geraldine Stock, Ursula Schmidt-Erfurth
2010 Investigative Ophthalmology and Visual Science  
PURPOSE. To evaluate the performance of automated analyses integrated in three spectral-domain optical coherence tomography (SD-OCT) devices to identify drusen in eyes with early (i.e., nonatrophic and nonneovascular) age-related macular degeneration (AMD). METHODS. Twelve eyes of 12 AMD patients, classified as AREDS 2 and 3 and having a mean count of 113 drusen were examined with three clinical SD-OCT devices (Cirrus [Carl Zeiss Meditec, Dublin CA], 3DOCT-1000 [Topcon, Tokyo, Japan], and
more » ... , Japan], and Spectralis [Heidelberg Engineering, GmbH, Heidelberg, Germany]) and five different scan patterns. After standard automated segmentation of the RPE was performed, every druse in each B-scan was identified and graded by two independent expert graders. Errors in the segmentation performance were classified as negligible, moderate, or severe. Correlations were based on the diameter and height of the druse and its automated segmentation. The overall drusen pattern identified by experts' detailed delineation was plotted with a custom-made computer program to compare automated to manual identification outcomes. RESULTS. A total of 1356 drusen were analyzed. The automated segmentation of the retinal pigment epithelium (RPE) by Cirrus made significantly fewer errors in detecting drusen than did the 3DOCT-1000 (P Ͻ 0.001). The Cirrus 200 ϫ 200 scan pattern detected 30% of the drusen with negligible errors. Spectralis did not offer a true RPE segmentation. The drusen counts by expert graders were significantly higher in the scans than in the standard fundus photographs (P Ͻ 0.05). CONCLUSIONS. SD-OCT imaging proved an excellent performance in visualizing drusen-related RPE disease. However, the available automated segmentation algorithms showed distinct limitations to reliable identification of the amount of drusen, particularly smaller drusen, and the actual size. (Invest Ophthalmol Vis Sci. 2010;51:6715-6721)
doi:10.1167/iovs.10-5288 pmid:21123769 fatcat:773xzkam6fe2zgzclcvdgfrb44

Vaccination with the immunoglobulin M-degrading enzyme of Streptococcus suis, Ide, leads to protection against a highly virulent serotype 9 strain

Karoline Rieckmann, Anna Seydel, Kristin Klose, Gottfried Alber, Christoph G. Baums, Nicole Schütze
2019 Vaccine: X  
Though fibrinous endocarditis x f placebo 0/9 6/9 3/9 1/9 g 0/9 0/9 3/9 1/9 1/9 2/9 0/9 0/9 6/9 1/9 0/9 0/9 1/9 0/9 3/9 1/9 3.0 h rIde Ssuis 3/9 2/9 0/9 0/9 2/9 0/9 1/9 0/9 1/9 3/9 0/9 0/9 0/9 6/9 0/9  ...  fibrinosuppurative inflammations. d Scoring of 2 and 3 indicates mild focal fibrinosuppurative inflammation. e Individual single perivascular neutrophils received a score of 1. f x = Rscore max /n animals . g  ... 
doi:10.1016/j.jvacx.2019.100046 pmid:31709420 pmcid:PMC6831886 fatcat:42weol5lijcdlh4eytuu5yorpy

Analysis of Porcine Pro- and Anti-Inflammatory Cytokine Induction by S. suis In Vivo and In Vitro

Florian S. Hohnstein, Marita Meurer, Nicole de Buhr, Maren von Köckritz-Blickwede, Christoph G. Baums, Gottfried Alber, Nicole Schütze
2020 Pathogens  
For functional studies, recombinant porcine TNF-α (10 ng/mL) or neutralizing anti-TNF-α antibody (4 µg/mL; both R&D Systems Inc., Minneapolis, MN, USA) was added.  ...  Isolation of PBMC, Monocytes, and Granulocytes PBMC were isolated from whole blood by density gradient centrifugation with Biocoll (1.077 g/mL; Biochrom/Merck KGaA, Darmstadt, BW, Germany).  ... 
doi:10.3390/pathogens9010040 pmid:31947746 pmcid:PMC7168595 fatcat:p7kucuqstvcszny5efvvhecxbe

A generic selection system for improved expression and thermostability of G protein-coupled receptors by directed evolution

Christoph Klenk, Janosch Ehrenmann, Marco Schütz, Andreas Plückthun
2016
With more than 800 members, G protein-coupled receptors (GPCRs) represent the largest superfamily of IMPs and are considered as one of the most important drug targets 1,2 .  ...  Structural and biophysical studies as well as drug screening approaches on G protein-coupled receptors (GPCRs) have been largely hampered by the poor biophysical properties and low expression yields of  ...  Cells were transformed with the respective plasmids by electroporation and were grown at 30 °C in 2× YT supplemented with 10 mg/mL glucose and 100 μ g/mL ampicillin.  ... 
doi:10.5167/uzh-125014 fatcat:lcu6itkjc5bvrn3m7p2efsvch4

Mycobacterium tuberculosis bloodstream infection prevalence, diagnosis, and mortality risk in seriously ill adults with HIV: a systematic review and meta-analysis of individual patient data

David A Barr, Joseph M Lewis, Nicholas Feasey, Charlotte Schutz, Andrew D Kerkhoff, Shevin T Jacob, Ben Andrews, Paul Kelly, Shabir Lakhi, Levy Muchemwa, Helio A Bacha, David J Hadad (+28 others)
2020 Lancet. Infectious Diseases (Print)  
Cohort 2012-13 Inpatients 9·6 427 338 Griesel et al (2017) South Africa Personal contact Yes Secondary care hospitals Cohort 2011-14 Inpatients with suspected tuberculosis 23·6 484 444 Schutz  ... 
doi:10.1016/s1473-3099(19)30695-4 pmid:32178764 pmcid:PMC7254058 fatcat:c6iq2dtlp5hclcqa2tt22ilgri

Frontmatter

2019 Random Operators and Stochastic Equations  
Christa, Ärztin, Mannheim; Schütz, Sonja, Berlin; Schütz, Juliane, Berlin; Schütz, Antje, Berlin; Schütz, Valentin, Mannheim; Seils, Dorothee, Apothekerin, Stuttgart; Seils, Gabriele, Journalistin, Berlin  ...  Annette, Ärztin, Berlin; Schütz, Dr.  ...  Girko The limit G-Law for the solutions of systems of linear algebraic equations with independent random coefficients under the G-Lindeberg condition 65  ... 
doi:10.1515/rose-2019-frontmatter1 fatcat:zgyouyam6za7lp2mfizy3x2suy

Frontmatter

2016 Klio : Beiträge zur alten Geschichte  
Collective Decision Making in the "Iliad" (Mait Kõiv) 309 Lynette G.  ...  Christa, Ärztin, Mannheim; Schütz, Sonja, Berlin; Schütz, Juliane, Berlin; Schütz, Antje, Berlin; Schütz, Valentin, Berlin; Seils, Dorothee, Apothekerin, Stuttgart; Seils, Gabriele, Journalistin, Berlin  ... 
doi:10.1515/klio-2016-frontmatter1 fatcat:4m3fssjglnfwlpft25crciaqta

Frontmatter

2018 Forum mathematicum  
Christa, Ärztin, Mannheim; Schütz, Sonja, Berlin; Schütz, Juliane, Berlin; Schütz, Antje, Berlin; Schütz, Valentin, Mannheim; Seils, Dorothee, Apothekerin, Stuttgart; Seils, Dr.  ...  Annette, Ärztin, Berlin; Schütz, Dr.  ... 
doi:10.1515/forum-2018-frontmatter1 fatcat:qlyei6fbe5h7lbdkjkowul4dtu

Frontmatter

2015 Journal of Applied Analysis  
Christa, Ärztin, Mannheim; Schütz, Sonja, Berlin; Schütz, Juliane, Berlin; Schütz, Antje, Berlin; Schütz, Valentin, Berlin; Seils, Dorothee, Apothekerin, Stuttgart; Seils, Gabriele, Journalistin, Berlin  ...  Annette, Ärztin, Berlin; Schütz, Dr.  ...  Volterra-Fredholm type integrodi erential third order dispersion equations 1 Youcef Mammeri Asymptotic behavior of small solutions of the Benjamin-Ono equation with time-dependent coe cients 9 Ekaterina G.  ... 
doi:10.1515/jaa-2015-frontmatter1 fatcat:z7im7uu3b5fajfsxjlikjpj6um

Masthead

2013 Journal of Homeland Security and Emergency Management  
Egnoto, Elena Svetieva, Arun Vishwanath and Christopher R.  ...  ; Valentin Schütz, Berlin.  ... 
doi:10.1515/jhsem-2013-masthead1 fatcat:4qnnilf5anefpl53zdvlaphcze

Zwei Faktoren für mehr Sicherheit

Hannes Rügheimer
2019 ästhetische dermatologie & kosmetologie  
Sicherheitsexperten empfehlen zum Schutz der Daten die Zwei-Faktor-Authentifizierung. Hannes Rügheimer Auch ein Smartphone kann als zweiter Faktor für mehr Sicherheit bei der Authenti zierung dienen.  ...  Datenschutzexperten wie Christoph Rösseler, Sprecher des Sicherheitssoware anbieters G-Data, weisen darauf hin, dass klassische Passwörter und PINs keinen ausreichenden Datenschutz bieten: "Um Onlinezugänge  ...  Gleichzeitig biete sie zuverlässigen Schutz gegen Angri e wie Phishing (E-Mails mit Schadprogrammen) oder manipulierte Webseiten ("HTML injection").  ... 
doi:10.1007/s12634-019-0064-1 fatcat:yk3fuxpsifbavm5yln4sez6xhq
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