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Conformational Consequences for Compatible Osmolytes on Thermal Denaturation

Nimesh Shukla, Brianna Bembenek, Erika A. Taylor, Christina M. Othon
2021 Life  
We found a slope of 11.0 +/−0.7 • C/M for trehalose and 8.8 +/−0.6 • C/M for maltose. Figure 4 . 4 Figure 4.  ...  We found a slope of 11.0 +/−0.7 °C/M for trehalose and 8.8 +/−0.6 °C/M for maltose. Figure 3 . 16 Figure 3 .Figure 4 . 31634 Figure 3.  ... 
doi:10.3390/life11121394 pmid:34947925 pmcid:PMC8708791 fatcat:7cprqqmcqbachhfm562gtqnrde

Sucralose Interaction with Protein Structures [article]

Nimesh Shukla, Enrico Pomarico, Cody J. S. Hecht, Erika A. Taylor, Majed Chergui, Christina M. Othon
2017 arXiv   pre-print
Sucralose does not appear to disturb the native state of proteins for moderate concentrations (<0.2 M) at room temperature.  ...  All samples were prepared in 0.05 M NaH2PO4 buffer solution at pH 7.5 with 10µM of CaCl2.  ...  We found that the solubility limit of sucralose is approximately 0.6 M, however, sucrose is soluble in water up to ~6 M.  ... 
arXiv:1707.04614v2 fatcat:4tvlyan4j5gdllmnbnpcg34gzq

Orientation and Oligomerization Specificity of the Bcr Coiled-Coil Oligomerization Domain†

Christina M. Taylor, Amy E. Keating
2005 Biochemistry  
Kim and M. Burgess for BCR-C and C-BCR. K. Taghizadeh, D. Pheasant, M. Ali, J. Glover, D. Pamuk, D. Lee, E. Oakes, G. Grigoryan and D.  ...  The following concentrations were used: BCR AP and BCR-C P (5 μM, 25 μM, and 50 μM), BCRA38L AP (25 μM and 50 μM), BCRA38L-C-cap (30 μM, 50 μM, and 100 μM), cap-C-BCRE52L (30 μM, 50 μM, and 100 μM).  ...  The first experiment was performed by mixing BCR AP (12.5 μM), C-BCR (12.5 μM), and BCR-C (12.5 μM) at pH 7.2 ( Figure 5B ).  ... 
doi:10.1021/bi051493t pmid:16331985 pmcid:PMC2526250 fatcat:gzj4x3xyuzaardwoogaqxorsfi

Hormonal Modulation of Guanyl Nucleotide Binding to Rat Luteal Membranes1

Patrick J. McIlroy, Christina M. Taylor
1988 Biology of Reproduction  
Nucleotide5 Kdb ED,,c AC activity hCG binding GMPPnP GTP GDP ITP 0.15 MM 0.16 0.56 22.6 0.25 M 0.25 ND 15.0 0.20 uM 0.65 0.65 75.0 Not Determined.  ...  Analysis of the data to obtain the various binding parameters gave an affinity of 1.8' 10' ± 0.5' 10' M-' , with a number of binding sites equal to 106 ± 14 fmol/mg wet wt. tissue in the absence of hCG  ... 
doi:10.1095/biolreprod38.5.1012 pmid:3408769 fatcat:lst3rxo3pbcvvl4ezfrdeyfupq

Growth and Laboratory Maintenance of Vibrio cholerae

Raquel M. Martinez, Christina J. Megli, Ronald K. Taylor
2010 Current Protocols in Microbiology  
publically available StrainSource TCP/CT Serogroup/Biotype Table 2 2 Antibiotic stock solutions for use with V. cholerae culturesNOTE: All antibiotics should be filter sterilized by passage through a 0.22 μm  ... 
doi:10.1002/9780471729259.mc06a01s17 pmid:20440684 pmcid:PMC4142487 fatcat:r3b4yu5jsrcpph4p6wxy7av2ta

Microfluidic Multicompartment Device for Neuroscience Research†

Anne M. Taylor, Seog Woo Rhee, Christina H. Tu, David H. Cribbs, Carl W. Cotman, Noo Li Jeon
2003 Langmuir  
(B) Langmuir, Vol. 19, No. 5, 2003 Taylor et al. Langmuir, Vol. 19, No. 5, 2003 Taylor et al. Langmuir, Vol. 19, No. 5, 2003 Taylor et al.  ...  The neuritic and somal compartments are connected by 120 grooves, 10 µm wide, 3 µm high, and 150 µm in length. The grooves are spaced 50 µm apart to prevent the grooves from collapsing.  ... 
doi:10.1021/la026417v pmid:20725530 pmcid:PMC2923462 fatcat:qfbsbndwtzazflm5zri2ms2xnq

A genome-wide Helicobacter pylori morphology screen uncovers a membrane spanning helical cell shape complex

Desirée C. Yang, Kris M. Blair, Jennifer A. Taylor, Timothy W. Petersen, Tate Sessler, Christina M. Tull, Christina Leverich, Amanda L. Collar, Timna J. Wyckoff, Jacob Biboy, Waldemar Vollmer, Nina R. Salama
2019 Journal of Bacteriology  
) genes, which contributes < 10% of the total coding sequence of the mitochondrial genome, are known as pathogenic hotspots because they are responsible for more than half of mtDNA-related diseases (Taylor  ...  Our affected child has the earliest disease onset compared to the other reported cases of m.4409T > C, m.4403T > C, m.4440G > A, m.4437C > T and m.4450G > A variants within the MT-TM gene; the clinical  ... 
doi:10.1128/jb.00724-18 pmid:31036730 pmcid:PMC6597387 fatcat:yr6jp7lh6fe6xmxrthpquumvfa

Invaders from islands: thermal matching, potential or flexibility?

Natalie M Claunch, Colin Goodman, Robert N Reed, Robert Guralnick, Christina M Romagosa, Emily N Taylor
2021 Biological Journal of the Linnean Society  
Neilson, M. Sandfoss and P. Miller for their assistance collecting lizards and thermal limit data. We also thank Florida Bay Interagency Science Center for allowing use of their facilities.  ...  M. Claunch pers. obs.). Leiocephalus carinatus thus represents a compelling case study for testing various thermal hypotheses for predicting areas of establishment for an island-sourced invader.  ...  Kearney & Porter, 2004 , 2009 Rödder et al., 2009; Wilms et al., 2011; reviewed by Taylor et al., 2020) , but fully fitting these models still often requires fine-grain microclimatic data.  ... 
doi:10.1093/biolinnean/blab103 fatcat:rkpfc436cnc5tceripvuxehwni

Patterned cell culture inside microfluidic devices

Seog Woo Rhee, Anne M. Taylor, Christina H. Tu, David H. Cribbs, Carl W. Cotman, Noo Li Jeon
2005 Lab on a Chip  
This paper describes a simple plasma-based dry etching method that enables patterned cell culture inside microfluidic devices by allowing patterning, fluidic bonding and sterilization steps to be carried out in a single step. This plasma-based dry etching method was used to pattern celladhesive and non-adhesive areas on the glass and polystyrene substrates. The patterned substrate was used for selective attachment and growth of human umbilical vein endothelial cells, MDA-MB-231 human breast
more » ... er cells, NIH 3T3 mouse fibroblasts, and primary rat cortical neurons. Finally, we have successfully combined the dry-patterned substrate with a microfluidic device. Patterned primary rat neurons were maintained for up to 6 days inside the microfluidic devices and the neurons' somas and processes were confined to the cell-adhesive region. The method developed in this work offers a convenient way of micropatterning biomaterials for selective attachment of cells on the substrates, and enables culturing of patterned cells inside microfluidic devices for a number of biological research applications where cells need to be exposed to wellcontrolled fluidic microenvironment.
doi:10.1039/b403091e pmid:15616747 fatcat:jgd5m6v74nc7fhfam3qcj2u6xi

Postacute Cognitive Rehabilitation for Adult Brain Tumor Patients

Christina Weyer-Jamora, Melissa S Brie, Tracy L Luks, Ellen M Smith, Shawn L Hervey-Jumper, Jennie W Taylor
2021 Neurosurgery  
Intrinsic brain tumors often occur within functional neural networks, leading to neurological impairment and disability of varying degrees. Advances in our understanding of tumor-network integration, human cognition and language processing, and multiparametric imaging, combined with refined intraoperative tumor resection techniques, have enhanced surgical management of intrinsic brain tumors within eloquent areas. However, cognitive symptoms impacting health-related quality of life,
more » ... processing speed, attention, concentration, working memory, and executive function, often persist after the postoperative recovery period and treatment. Multidisciplinary cognitive rehabilitation is the standard of care for addressing cognitive impairments in many neurological diseases. There is promising research to support the use of cognitive rehabilitation in adult brain tumor patients. In this review, we summarize the history and usefulness of postacute cognitive rehabilitation for adult brain tumor patients.
doi:10.1093/neuros/nyaa552 pmid:33586764 pmcid:PMC8600173 fatcat:jtscmg5wrzd3tmod4gmthh5lvm

Longitudinal Changes of the Ruminal Microbiota in Angus Beef Steers

Jeferson M. Lourenco, Taylor R. Krause, Christina B. Welch, Todd R. Callaway, T. Dean Pringle
2022 Animals  
Each PCR reaction contained a DNA template (12.5 ng), 5 µL forward primer (1 µM), 5 µL reverse primer (1 µM), 12.5 µL 2× Kapa HiFi Hotstart ready mix (Roche Molecular Systems, Inc., Indianapolis, IN, USA  ...  Briefly, 0.33 g of sample was placed into a Lysing Matrix E Tube (MP Biomedicals, Solon, OH, USA), which was homogenized in a FastPrep 24 homogenizer (MP Biomedicals, LLC, Irvine, CA, USA) at 6.0 m/s for  ... 
doi:10.3390/ani12091066 pmid:35565493 fatcat:xh2337nznzavtksz3gspubdsri

Expressive Art to Facilitate the Development of the Occupational Profile: A Scoping Review

Verna G Eschenfelder, Christina M Gavalas, Taylor M Pendergast, Colleen A Gorman
2018 Open Journal of Occupational Therapy  
search of 11 interdisciplinary databases available through the University of Scranton Library, including CINAHL, COCHRANE Library, ERIC (EBSCO), JAMA Network, JSTOR, PEDro, PubMed Central, Sage Premier, Taylor  ... 
doi:10.15453/2168-6408.1360 fatcat:wxytmrlyunhcnf4ymo6uiqbcpy

Myriaporone 3/4 structure–activity relationship studies define a pharmacophore targeting eukaryotic protein synthesis

John Hines, Myriam Roy, Hua Cheng, Christina M. Agapakis, Richard Taylor, Craig M. Crews
2006 Molecular Biosystems  
S. pombe was spotted onto YES plates (without or with 2 µM myriaporone 3/4) and S. cerevisiae were spotted onto YPD plates (without or with 2 µM myriaporone 3of three independent experiments (error bars  ...  Concentrations as low as 0.2 µM myriaporone 3/4 achieved a similar protein synthesis inhibition as a 100-fold greater concentration of cycloheximide.  ... 
doi:10.1039/b602936a pmid:16880957 pmcid:PMC2507749 fatcat:sztqxu3klvbqnfnw2y556pkb44

Hydrophobic interactions of sucralose with protein structures

Nimesh Shukla, Enrico Pomarico, Cody J.S. Hecht, Erika A. Taylor, Majed Chergui, Christina M. Othon
2018 Archives of Biochemistry and Biophysics  
Sucralose does not appear to disturb the native state of proteins for moderate concentrations (< 0.2 M) at room temperature.  ...  All samples were prepared in 0.05 M NaH 2 PO 4 buffer solution at pH 7.5 with 10 μM of CaCl 2 .  ...  We found that the solubility limit of sucralose is approximately 0.6 M, however, sucrose is soluble in water up to ∼6 M.  ... 
doi:10.1016/j.abb.2017.12.013 pmid:29288052 fatcat:bbx3xw26mvgcpif4yxqma52pp4

Abstract 121

Thomas Q. Xu, Taylor J. Jarazcewski, Aaron L. Wiegmann, Ethan M. Ritz, Todd Beck, Carlos A. Q. Santos, Christina Tragos, Amir H. Dorafshar
2019 Plastic and Reconstructive Surgery, Global Open  
Cooney, MD, PhD, Carisa M. Cooney, MPH, Gedge D. Rosson, MD The Johns Hopkins University, Baltimore, MD, USA  ...  of these socioeconomic and sex disparities. 122 Depression and Anti-depressant Therapy Effects on Quality of Life and Patient Satisfaction Outcome Measures in Breast Cancer Using the BREAST-Q Kevin M.  ... 
doi:10.1097/01.gox.0000558395.76726.8e fatcat:vqnsxeukhndd7h5slzqeinxfpq
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