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Charge as a Selection Criterion for Translocation through the Nuclear Pore Complex
2010
PLoS Computational Biology
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Citation: Colwell LJ, Brenner MP, Ribbeck K (2010) Charge as a Selection Criterion for Translocation through the Nuclear Pore Complex. PLoS Comput Biol 6(4): e1000747. ...
We suggest that negative charge is an essential criterion for selective passage through the NPC. ...
Acknowledgments We thank Jacob Israelachvili, Andrew Murray, Carey Nadell, Erin O'Shea, Michael Rexach, Thomas Schwartz and Boris Shraiman, for important conversations.
Author Contributions ...
doi:10.1371/journal.pcbi.1000747
pmid:20421988
pmcid:PMC2858669
fatcat:nk4l4ndmpjbffequghz3vyd2uq
Effect of charge, hydrophobicity, and sequence of nucleoporins on the translocation of model particles through the nuclear pore complex
2013
Proceedings of the National Academy of Sciences of the United States of America
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for selective transport through the pore (10-14) . ...
The molecular structure of the yeast nuclear pore complex (NPC) and the translocation of model particles have been studied with a molecular theory that accounts for the geometry of the pore and the sequence ...
for selective transport through the pore (10) (11) (12) (13) (14) . ...
doi:10.1073/pnas.1212909110
pmid:23404701
pmcid:PMC3587244
fatcat:d3kjnie6lnf3jn2vg55jhccpou
Role of Molecular Charge in Nucleocytoplasmic Transport
2014
PLoS ONE
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Recently, negative surface charge was postulated to be another essential criterion for selective passage through the NPC. ...
Transport of genetic materials and proteins between the nucleus and cytoplasm of eukaryotic cells is mediated by nuclear pore complexes (NPCs). ...
Cronican for providing the plasmids and purification protocols for charged GFPs, and thank S. Wunder and J. Drazenovic for their help in the measurement of dynamic light scattering. ...
doi:10.1371/journal.pone.0088792
pmid:24558427
pmcid:PMC3928296
fatcat:xf2ibnch5vfh5cdn76d45yt4sm
Viruses Challenge Selectivity Barrier of Nuclear Pores
2013
Viruses
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Exchange between the nucleus and the cytoplasm occurs through nuclear pore complexes (NPCs) embedded in the double membrane of the nuclear envelope. ...
In this review, we will discuss the functional principles of NPC barrier and nuclear transport machinery, as well as the various strategies viruses use to cross the selective barrier of NPCs. ...
Thus, the main sorting criterion of NPCs' barrier is partitioning into a selective phase that is a good "solvent" for NTRs, but not for polar inert macromolecules. ...
doi:10.3390/v5102410
pmid:24084236
pmcid:PMC3814595
fatcat:yktp3x6v4bd4fcgmnsyplwbyiy
The permeability barrier of nuclear pore complexes appears to operate via hydrophobic exclusion
2002
EMBO Journal
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Nuclear pore complexes (NPCs) restrict the nucleo-cytoplasmic¯ux of most macromolecules, but permit facilitated passage of nuclear transport receptors and their cargo complexes. ...
We found that a simple hydrophobic interaction column can mimic the selectivity of NPCs surprisingly well and that nuclear transport receptors appear to be the most hydrophobic soluble proteins. ...
the MBP±Ran constructs, and the DFG for ®nancial support. ...
doi:10.1093/emboj/21.11.2664
pmid:12032079
pmcid:PMC126029
fatcat:442666afqbawrb4iuj3eg66ldm
Translocation, Rejection and Trapping of Polyampholytes
2022
Polymers
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We investigated the translocation behavior of random polyampholyte chains through a pore under the action of an electric field by means of Monte Carlo simulations. ...
We also provided operational criteria for the translocation behavior of a sequence, explaining the selection by the translocation process. ...
In nature, charged polymers (proteins, RNA) are imported in or exported from the cell nucleus by translocation through a nuclear pore [12] . ...
doi:10.3390/polym14040797
pmid:35215709
pmcid:PMC8877523
fatcat:vvojv7blancavhcz42b5xni2wu
A coarse-grained computational model of the nuclear pore complex predicts Phe-Gly nucleoporin dynamics
2017
The Journal of General Physiology
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The phenylalanine-glycine–repeat nucleoporins (FG-Nups), which occupy the lumen of the nuclear pore complex (NPC), are critical for transport between the nucleus and cytosol. ...
Transport through the pore is on the millisecond timescale. Here, to explore the dynamics of nucleoporins on this timescale, we use coarse-grained computational simulations. ...
I n t R o d u c t I o n In all eukaryotes, nuclear pore complexes (NPCs) facilitate the transport of biomolecular cargo across the barrier of the nuclear envelope. ...
doi:10.1085/jgp.201711769
pmid:28887410
pmcid:PMC5694938
fatcat:5jwpplwzf5elnhtjqyp2cgiqpu
Kinetic analysis of translocation through nuclear pore complexes
2001
EMBO Journal
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The mechanism of facilitated translocation through nuclear pore complexes (NPCs) is only poorly understood. Here, we present a kinetic analysis of the process using various model substrates. ...
We propose a'selective phase model' that could explain how NPCs function as a permeability barrier for inert molecules and yet become selectively permeable for nuclear transport receptors and receptor± ...
Acknowledgements We thank Drs M.Pool, T.A.Rapoport, I.Vetter and D.Oesterhelt, the members of our laboratory, I.W.Mattaj and the anonymous reviewers for stimulating discussions/constructive comments on ...
doi:10.1093/emboj/20.6.1320
pmid:11250898
pmcid:PMC145537
fatcat:wxplbptitvccxbpr6hohlkyhbq
Transport receptor occupancy in Nuclear Pore Complex mimics
[article]
2021
bioRxiv
pre-print
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Nuclear Pore Complexes (NPCs) regulate all molecular transport between the nucleus and the cytoplasm in eukaryotic cells. ...
: one population that is transported across the pore in milliseconds, and a second population that is stably assembled within the FG mesh of the pore. ...
Acknowledgements We would like to thank the Görlich Lab for sharing purified Nsp1, Meng-yue Wu for technical assistance on the TEM, and Marc op den Kamp, Sjoerd Meesters and Koen Wortelboer for their assistance ...
doi:10.1101/2021.12.22.473839
fatcat:tvpz7zcai5dqhahtw6hebj7zqy
The particle in the spider's web: transport through biological hydrogels
2017
Nanoscale
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Abstract Biological hydrogels such as mucus, extracellular matrix, biofilms, and the nuclear pore have diverse functions and compositions, but all act as selectively permeable barriers to the diffusion ...
Graphical abstract Transport through biological hydrogels is an important biological process and of fundamental importance for drug delivery. ...
Alan Grodzinsky at MIT for his valuable feedback and helpful discussions, and Erica Shapiro, Wes Chen, Tahoura Samad, Caroline Wagner, Kathryn Smith-Dupont, and Lisa Walker for careful reading of the manuscript ...
doi:10.1039/c6nr09736g
pmid:28580973
pmcid:PMC5841163
fatcat:2bdhnej6tjat5bmtnxrgfq3lxy
Nucleocytoplasmic transport of intrinsically disordered proteins studied by high-speed super-resolution microscopy
2020
Protein Science
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Both natively folded and intrinsically disordered proteins (IDPs) destined for the nucleus need to transport through the nuclear pore complexes (NPCs) in eukaryotic cells. ...
Our data revealed that the rules executed for folded proteins are not well followed by the IDPs. Instead, both large and small IDPs can passively diffuse through the NPCs. ...
ACKNOWLEDGMENTS We greatly thank Vladimir Uversky (University of South Florida, Tampa, USA) for providing helpful information on IDP characteristics to aid in our protein selections. We thank M. ...
doi:10.1002/pro.3845
pmid:32096308
fatcat:baqzdwfahvddtless7zp5da2im
Mechanism of Tc toxin action revealed in molecular detail
2014
Nature
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TcA forms a large bell-shaped pentameric structure 5,10,11 and enters the membrane like a syringe, forming a translocation channel through which the cytotoxic domain is probably transported into the cytoplasm ...
This unique mechanism differs from that of typical pore-forming toxins 12 or other toxins that form translocation pores, for example, diphtheria 13 and anthrax toxin 14 . ...
Bergbrede for technical support and the Raunser laboratory for selecting particles. ...
doi:10.1038/nature13015
pmid:24572368
fatcat:ik4vew5gyndfzm27leokq4ebzm
Transmembrane protein rotaxanes reveal kinetic traps in the refolding of translocated substrates
2020
Communications Biology
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Here, we describe a single-molecule method to characterize the folded state of individual proteins after membrane translocation, by monitoring the ionic current passing through the pore. ...
We trap the protein in the nanopore as a rotaxane-like complex using streptavidin stoppers. The protein is subjected to cycles of unfolding-translocation-refolding switching the voltage polarity. ...
Jonathan Hopper and Professor Carol Robinson for the MS of the construct oligo(dC) 30 -V5-oligo(dA) 30 ...
doi:10.1038/s42003-020-0840-5
pmid:32246060
fatcat:ro2n5a4msbf5vl4x5tq3y4szvy
Systematic analysis of barrier-forming FG hydrogels from Xenopus nuclear pore complexes
2012
EMBO Journal
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Nuclear pore complexes (NPCs) control the traffic between cell nucleus and cytoplasm. ...
While facilitating translocation of nuclear transport receptors (NTRs) and NTR . cargo complexes, they suppress passive passage of macromolecules X30 kDa. ...
Acknowledgements We wish to thank Jü rgen Schü nemann and Uwe Plemann for excellent technical support; Koray Kirli for the pKK vectors; Maarten Fornerod for a xlNup214 clone; Irene Böttcher-Gajewski for ...
doi:10.1038/emboj.2012.302
pmid:23202855
pmcid:PMC3553378
fatcat:njjqbdljujeijc6gjj5xeh2hsu
Clustering by common friends finds locally significant proteins mediating modules
2007
Bioinformatics
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We apply the algorithm to a yeast network. Results: Clusters and mediators are organized in hierarchies, where clusters are mediated by and act as mediators for other clusters. ...
We illustrate the above findings with an example of membrane protein translocation from the cytoplasm to the inner nuclear membrane. Availability: All software is freely available under Supplementary ...
ACKNOWLEDGEMENTS The authors are grateful for the comments of Christof Winter, the financial support of the Natural Sciences and Engineering Research Council (NSERC), the Ontario Graduate Scholarship Program ...
doi:10.1093/bioinformatics/btm064
pmid:17314122
fatcat:e2qs6bp34rhojhr26qumabalbe
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