A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is application/pdf.
Filters
Protein structure characterization with mass spectrometry
2004
Spectroscopy (Ottawa)
Preserved Fulltext
Mass spectrometry is now commonly being used to determine both the primary and higher order structures of proteins. ...
The basis for these investigations lies in the ability of mass analysis techniques to detect changes in protein conformation under differing conditions. ...
Chemical cross-linking and chemical modification Other methods of probing higher-order structure include investigating the chemical reactivity of individual amino acids in a protein and chemical cross-linking ...
doi:10.1155/2004/407960
fatcat:cipizmlayrg3pfoa54bkq3dwki
Mass spectrometry for semi-experimental protein structure determination and modeling
[article]
2020
arXiv
pre-print
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
The file type is application/pdf.
We review the methodology and describe software programs supporting such integrated protein structure prediction approaches, making use of distance constraints got from mass spectrometry cross-linking ...
Then we describe how mass spectrometry is useful for semi-experimental integrative protein structure determination. ...
The advancement of chemical cross-linking and mass spectrometry for structural proteomics: from single proteins to protein interaction networks. ...
arXiv:2003.00328v1
fatcat:er2hzplirjgenpwla6wresatce
Subject index
2004
Journal of the American Society for Mass Spectrometry
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is application/pdf.
characterization of thiols, 288 Tryptophan, protonated, gas phase reactions of, 65 Tyrosine, chemical cross-linking with lysine, 1604 Ultraviolet-MALDI matrices, liquid based, detection limits of proteins ...
energy in, 48 Quadrupole tandem mass spectrometry (MS/MS), triple, in molecular characterization of thiols, 288 Quadrupole time-of-flight mass spectrometer, CID in, generating protein sequence tags by, ...
doi:10.1016/s1044-0305(04)00746-9
fatcat:7gg3qm3w5bblrphu6mnr3ygcbe
Editorial overview: Carbohydrates: Ménage à trois with glycosaminoglycans — a serious rendezvous, not a gag!
2018
Current Opinion in Structural Biology
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
The file type is application/pdf.
They provide evidence that immobilized GAGs can form mechanically soft and swollen brushes that can be cross-linked by binding proteins. ...
of the buiding blocks, sulfation pattern), the conformation and the dynamics of these molecules. ...
doi:10.1016/j.sbi.2018.07.004
pmid:30080481
fatcat:bj4gx6ebhzakxi2gfonfxfnrmm
Mass spectrometry of glycans
2013
Biochemistry (Moscow)
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2017; you can also visit the original URL.
The file type is application/pdf.
They are involved in diverse roles such as molecular recognition, cell-cell and cell-matrix interactions, energy generation, and modification of protein conformations. ...
Monosaccharides constitute oligosaccharides (2-10 units) Abstract-Powerful new strategies based on mass spectrometry are revolutionizing the structural analysis and profiling of glycans and glycoconjugates ...
When linked to a specific site on a protein backbone, glycans can modify the structural properties of the protein, affecting its solubility and conformation. ...
doi:10.1134/s0006297913070031
pmid:24010834
pmcid:PMC4104780
fatcat:iddoudix3bgzzcmjhoe6ziu76e
Characterization of simple isomeric oligosaccharides and the rapid separation of glycan mixtures by ion mobility mass spectrometry
2010
International Journal of Mass Spectrometry
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2015; you can also visit the original URL.
The file type is application/pdf.
The ion mobility mass spectrometry techniques were used to separate the isomeric oligosaccharides and the glycan mixtures. ...
of isomeric oligosaccharides and hydrazine-released N-linked glycans from various sources. ...
Acknowledgments The support of the Department of Food and Rural Affairs, UK, is gratefully acknowledged (JHS and MTB) as is the National Science Foundation, USA (MTB) and the Oxford Glycobiology Institute ...
doi:10.1016/j.ijms.2009.08.006
fatcat:hdwtsgoyjndndhpnn77ogqceyu
Use of Proteinase K Nonspecific Digestion for Selective and Comprehensive Identification of Interpeptide Cross-links: Application to Prion Proteins
2012
Molecular & Cellular Proteomics
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2018; you can also visit the original URL.
The file type is application/pdf.
Cross-linked proteins are usually digested with trypsin to generate cross-linked peptides, which are then analyzed by mass spectrometry. ...
Chemical cross-linking combined with mass spectrometry is a rapidly developing technique for structural proteomics. ...
For mass spectrometric determination of the crosslinking sites, the cross-linked protein is typically digested with proteolytic enzymes, and the peptides are examined by mass spectrometry. ...
doi:10.1074/mcp.m111.013524
pmid:22438564
pmcid:PMC3394941
fatcat:dz5qajqlozft5eq7qz3uxvasnq
Chemical cross-linking in the structural analysis of protein assemblies
2018
Methods
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
The file type is application/pdf.
The chemical cross-linking approach has recently been revived by mass spectrometric analysis of the cross-linking reaction products. ...
Selection of proper chemical cross-linking reagents, strategies for crosslinked peptide identification, and successful application of CXMS in structural characterization of proteins and protein complexes ...
Acknowledgments This work was supported by grants from the UNH Collaborative Research Excellence (CoRE), National Science Foundation (CLF 1307367) and the National Institute of Health (R01 HD093783). ...
doi:10.1016/j.ymeth.2018.05.023
pmid:29857191
pmcid:PMC6051896
fatcat:sz7v2pmhxvglhexio6lswexzku
Characterization of protein glycosylation using chip-based infusion nanoelectrospray linear ion trap tandem mass spectrometry
2004
Journal of Biomolecular Techniques
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
The file type is application/pdf.
Mass spectrometry (MS) has the potential to revolutionize structural glycobiology and help in the understanding of how post-translation events such as glycosylation affect protein activities. ...
of the linear ion trap and the extended analysis time provided by the ionization technique. ...
We also thank Professor Jack Henion and Dr. Colleen Van Pelt for reviewing this manuscript and for providing helpful comments. ...
pmid:15190085
pmcid:PMC2291675
fatcat:sufg33gqwbemnnwzyqfpoys5ze
Page 549 of Journal of Mass Spectrometry Vol. 37, Issue 5
[page]
2002
Journal of Mass Spectrometry
Preserved Fulltext
The Internet Archive has digitized a microfilm copy of this work. It may be possible to borrow a copy for reading.
Proteins, Tissue Proteomics Unit, Bethesda, Md 20892, USA
Dis Marker 2001 17 (4) 301
Intramolecular cross-linking experiments on cytochrome c and ribonuclease A using an isotope multiplet method
Pearson ...
: Characterization by proteolytic degradation of monoclonal antibody-bound antigen and mass spectrometry
Michel S, Forest E, Petillot Y, Deleage G, Heuze-Vourch N, Courty Y, Lascoux D, Jolivet M, Jolivet ...
Amino Acid Proximities in Two Sup35 Prion Strains Revealed by Chemical Cross-linking
2015
Journal of Biological Chemistry
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2018; you can also visit the original URL.
The file type is application/pdf.
Intra-and intermolecular cross-linking were distinguished by tandem mass spectrometry analysis on fibers seeded from solutions containing equal amounts of 14 N-and 15 N-labeled protein. ...
A, chain folding topology of the VL fiber constrained by cross-linking between residue 3 and 28 and residues 32 and 55. ...
The cross-linking induced by UV irradiation was subsequently analyzed by tandem mass spectrometry. ...
doi:10.1074/jbc.m115.676379
pmid:26265470
pmcid:PMC4599010
fatcat:xib7f6meyfe33jqz4hmei3dbpq
In vivo Cross-Linking MS of the Complement System MAC Assembled on Live Gram-Positive Bacteria
2021
Frontiers in Genetics
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2021; you can also visit the original URL.
The file type is application/pdf.
Protein cross-linking combined with mass spectrometry (MS) and computational modeling is gaining increased recognition as a viable tool in protein interaction studies. ...
Protein–protein interactions are central in many biological processes, but they are challenging to characterize, especially in complex samples. ...
Here, we applied a simplified version (see the Materials and Methods section for details) of the recently developed targeted cross-linking mass spectrometry (TX-MS) approach to study the formation of ...
doi:10.3389/fgene.2020.612475
pmid:33488677
pmcid:PMC7820895
fatcat:bfsuuo6f2fcdnm722lvxvqp6oa
BEHIND THE SCENES OF HOST-MICROBE INTERACTIONS
2020
Military Medical Science Letters
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
The file type is application/pdf.
To increase their sensitivity and accuracy, these methods are commonly used in combinations, such as affinity purification and liquid chromatographymass spectrometry analysis, cross-linking together with ...
liquid chromatography-mass spectrometry analysis, or stable isotope labeling with amino acids in cell culture with affinity purification. ...
Protein complexes are purified (by immunopurification) and followed by enzymatic digestion and mass spectrometry analysis after cross-linking reaction. ...
doi:10.31482/mmsl.2020.010
fatcat:jlemtmdbvfa2pnc4uobdgqorxm
Eisosomes and Plasma Membrane Domain Formation
2014
Biophysical Journal
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is application/pdf.
We then compare that information with that garnered from intrinsic tyrosine absorbance to understand the changes in structural conformations as a function of the mutation and lipid binding. ...
Amots are adaptor proteins which coordinate signaling for cellular differentiation and proliferation. Their ACCH domain binds lipids with specificity leading to membrane deformation. ...
Here, we describe an integrative approach to determine the topologies of heteromeric assemblies using structural information derived from native mass spectrometry (MS), proteomics and chemical cross-linking ...
doi:10.1016/j.bpj.2013.11.2636
fatcat:vaypbd6efvdxtmmpigcq6xlzeu
Lipidated apolipoprotein E4 structure and its receptor binding mechanism determined by a combined cross-linking coupled to mass spectrometry and molecular dynamics approach
2018
PLoS Computational Biology
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is application/pdf.
Chemical cross-linking combined with mass spectrometry provided distance restraints, characterizing the three-dimensional organization of apoE4 molecules at the surface of lipidic nanoparticles. ...
Overall, our results show how apoE4 adapts its conformation for the recognition of the low density lipoprotein receptor and we propose a novel mechanism of activation for apoE4 that is based on accessibility ...
The advancement of chemical cross-linking and mass spectrometry for structural proteomics: from single proteins to protein interaction networks. ...
doi:10.1371/journal.pcbi.1006165
pmid:29933361
pmcid:PMC6033463
fatcat:up63t2cfbvatbkokwkf76vk5ky
« Previous
Showing results 1 — 15 out of 11,543 results