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Chaos in Air Pollutant Concentration (APC) Time Series

Chung-Kung Lee, Shu-Chen Lin
2008 Aerosol and Air Quality Research  
Some problems concerning the applicability of chaos analysis in air pollution are also discussed.  ...  The presence of chaos in the examined APC time series is evident with the low correlation dimensions (3.42-4.71), the positive values of the largest Lyapunov exponent (0.128-0.427), and the positive Kolmogorov  ...  ., 2004; Wang and Chen, 2008; Yang et al., 2008) . Air quality changes related to human action can be investigated by long-term and large-area monitoring.  ... 
doi:10.4209/aaqr.2008.09.0039 fatcat:hkod67oelvgdrhxaw76jccvcda

Iatrogenic invasive otomycosis

Chao-Lan Hsu, Cheng-Wei Chen, Hua-Kung Wang
2011 Tzu-Chi Medical Journal  
Chen).  ...  Chen et al. [2] classified fungal infections of the ear and temporal bone infections into four types according to extension of the disease process and treatment ( Table 1) .  ... 
doi:10.1016/j.tcmj.2011.01.012 fatcat:6dpe5i2dv5ae3ptljyv7rouusa

Subtypes of physical frailty: Latent class analysis and associations with clinical characteristics and outcomes

Li-Kuo Liu, Chao-Yu Guo, Wei-Ju Lee, Liang-Yu Chen, An-Chun Hwang, Ming-Hsien Lin, Li-Ning Peng, Liang-Kung Chen, Kung-Yee Liang
2017 Scientific Reports  
Chen L.K. and Liang K.Y. participated in the design of the work and drafting the article. Chen L.Y., Huang A.C., Lin M.H., and Peng L.N. provided critical reviews.  ... 
doi:10.1038/srep46417 pmid:28397814 pmcid:PMC5387710 fatcat:ntgq5vr4vjfarlp4o45mcd3uhy

Feature-Rich Magnetic Quantization in Sliding Bilayer Graphenes [article]

Yao-Kung Huang, Szu-Chao Chen, Yen-Hung Ho, Chiun-Yan Lin, Ming-Fa Lin
2014 arXiv   pre-print
The generalized tight-binding model, based on the subenvelope functions of distinct sublattices, is developed to investigate the magnetic quantization in sliding bilayer graphenes. The relative shift of two graphene layers induces a dramatic transformation between the Dirac-cone structure and the parabolic band structure, and thus leads to drastic changes of Landau levels (LLs) in the spatial symmetry, initial formation energy, intergroup anti-crossing, state degeneracy and semiconductor-metal
more » ... ransition. There exist three kinds of LLs, i.e., well-behaved, perturbed and undefined LLs, which are characterized by a specific mode, a main mode plus side modes, and a disordered mode, respectively. Such LLs are clearly revealed in diverse magneto-optical selection rules. Specially, the undefined LLs frequently exhibit intergroup anti-crossings in the field-dependent energy spectra, and show a large number of absorption peaks without optical selection rules.
arXiv:1408.3827v1 fatcat:nlktojjg5jaljnzew4zixvy72u

Decreased PEDF Expression Promotes Adipogenic Differentiation through the Up-Regulation of CD36

Kuang-Tzu Huang, Li-Wen Hsu, Kuang-Den Chen, Chao-Pin Kung, Shigeru Goto, Chao-Long Chen
2018 International Journal of Molecular Sciences  
Adipogenesis is a tightly regulated cellular process that involves the action of multiple signaling pathways. Characterization of regulators that are associated with adipose development is crucial to understanding the mechanisms underlying obesity and other metabolic disorders. Pigment epithelium-derived factor (PEDF) is a secreted glycoprotein that was first described as a neurotrophic factor. The role of PEDF in lipid metabolism was established when adipose triglyceride lipase (ATGL), a major
more » ... triglyceride hydrolase, was characterized as its binding partner. In this study, we investigated the downstream effects of PEDF on adipogenic differentiation using rat adipose-derived stem cells (AdSCs) and the mouse pre-adipocyte cell line 3T3-L1. Knocking down PEDF in differentiating cells resulted in elevated levels of ATGL and CD36, as well as other adipogenic markers, with a concomitant increase in adipocyte number. CD36, a scavenger receptor for a variety of ligands, regulated proliferation and lipogenic gene expression during adipogenesis. The CD36 increase due to PEDF down-regulation might be a result of elevated PPARγ. We further demonstrated that PEDF expression was regulated by dexamethasone, a synthetic glucocorticoid that is widely used for adipogenesis at the transcriptional level. Taken together, our findings highlight that PEDF negatively regulates adipogenesis through the regulation of various signaling intermediates, and it may play a crucial role in lipid metabolic disorders.
doi:10.3390/ijms19123992 fatcat:xnwv53kzw5fzlb6rdakhjm5ite

Decreased PEDF Promotes Hepatic Fatty Acid Uptake and Lipid Droplet Formation in the Pathogenesis of NAFLD

Kuang-Tzu Huang, Kuang-Den Chen, Li-Wen Hsu, Chao-Pin Kung, Shu-Rong Li, Chien-Chih Chen, King-Wah Chiu, Shigeru Goto, Chao-Long Chen
2020 Nutrients  
Non-alcoholic fatty liver disease (NAFLD), the leading cause of chronic liver diseases worldwide, ranges from simple steatosis to steatohepatitis, with the risk for progressive fibrosis or even cirrhosis. While simple steatosis is a relatively benign condition, the buildup of toxic lipid metabolites can induce chronic inflammation, ultimately triggering disease progression. Pigment epithelium-derived factor (PEDF) is a secreted, multifunctional glycoprotein with lipid metabolic activities. PEDF
more » ... promotes lipolysis through binding to adipose triglyceride lipase (ATGL), a key enzyme for triglyceride breakdown. In the current study, we aimed to delineate how changes in PEDF expression affect hepatic lipid accumulation. Our data revealed that hepatic PEDF was downregulated in a mouse NAFLD model. We further showed that decreased PEDF levels in hepatocytes in vitro resulted in elevated fatty acid uptake and lipid droplet formation, with concomitant upregulation of fatty acid transport proteins CD36 and fatty acid binding protein 1 (FABP1). RNA sequencing analysis of PEDF knocked down hepatocytes revealed an alteration in gene expression profile toward lipid accumulation. Additionally, decreased PEDF promotes mobilization of fatty acids, an observation distinct from blocking ATGL activity. Taken together, our data suggest that hepatic PEDF downregulation causes molecular changes that favor triglyceride accumulation, which may further lead to NAFLD progression.
doi:10.3390/nu12010270 pmid:31968655 pmcid:PMC7019565 fatcat:qlgiz4o6wjdlbmwem7wpcd6vxm

PKCalpha mediated induction of miR-101 in human hepatoma HepG2 cells

Chao-Wei Chiang, Yi Huang, Ka-Wai Leong, Lih-Chyang Chen, Hua-Chien Chen, Shu-Jen Chen, Chen-Kung Chou
2010 Journal of Biomedical Science  
Protein Kinase C (PKC) is a serine/threonine kinase that involved in controlling of many cellular processes such as cell proliferation and differentiation. We have observed previously that TPA (12-O-tetradecanoylphorbol 13acetate) induces cell cycle arrest in G0/G1 phase in human hepatoma HepG2 cells. However, is there any miRNA involved in PKCα mediated cell growth arrest is still unknown. Methods: We first surveyed 270 miRNA expression profiles in 20 pairs of human hepatoma tissues. We
more » ... ied 11 upregulated and 23 down-regulated miRNAs (FDR < = 0.01; fold-change > = 2) in human hepatoma tissue after Student's T-test and Mann-Whitney rank test. We then examined miRNAs expression profile in TPA treated HepG2 cells. Two miRNAs, miR-101, and miR-29c, were shown to be significantly down regulated in human hepatoma tissues and induced over 4-fold in HepG2 cells under TPA treatment. Results: In this study, we examined TPA regulated miRNA expression profile in human hepatoma HepG2 cells. We identified two miRNAs, 101 and 29c, were induced by TPA and down regulated in human hepatoma tissues suggest that they might play as tumor suppressor gene and in tumor formation of HCC. Since induction kinetics of miR-101 by TPA was much faster than miR-29c suggests that the induction of miR-101 may be the primary response of TPA treatment. We then further investigated how miR-101 was regulated by TPA. MiR-101 targets two subunits of PRC2 complex, enhancer of zeste homolog 2 (EZH2) and EED, and was shown to play as a tumor suppressor gene in human prostate, breast and liver cancers. The target sequence of miR-101 located in the 3' UTR of both EZH2 and EED's mRNA was identified by bioinformatic analysis and was validated by reporter luciferase activity assay. Then we showed that TPA not only up regulated miR-101 expression, but also reduced protein level of EZH2, EED and H3K27me3 in HepG2 cells. Using lenti-virus-mediated shRNA to knockdown endogenous PKCα expression, we observed that TPA induced growth arrest, elevation of miR-101 and reduction of EZH2, EED and H3K27me3 proteins were all PKCα dependent. Specific inhibitor of ERK completely blocked TPA induced miR-101 expression. Conclusions: Therefore, this is the first time to show that PKCα and ERK pathway play important role to activate miR-101 expression, reduce PRC2 complex and H3K27me3 level. This epigenetic regulatory pathway may represent a novel mechanism of carcinogenesis and deserve further investigation.
doi:10.1186/1423-0127-17-35 pmid:20444294 pmcid:PMC2874775 fatcat:cv4drxcp2jbhdiy25ubgpupcqq

Levetiracetam in the Treatment of Epileptic Seizures After Liver Transplantation

Chih-Hsiang Lin, Chao-Long Chen, Tsu-Kung Lin, Nai-Ching Chen, Meng-Han Tsai, Yao-Chung Chuang
2015 Medicine  
After liver transplantation, patients may develop seizures or epilepsy due to a variety of etiologies. The ideal antiepileptic drugs for these patients are those with fewer drug interactions and less hepatic toxicity. In this study, we present patients using levetiracetam to control seizures after liver transplantation. We retrospectively enrolled patients who received levetiracetam for seizure control after liver transplantation. We analyzed the etiology of liver failure that required liver
more » ... nsplantation, etiology of the seizures, outcomes of seizure control, and the condition of the patient after follow-up at the outpatient department. Hematological and biochemical data before and after the use of levetiracetam were also collected. Fifteen patients who received intravenous or oral levetiracetam monotherapy for seizure control after liver transplantation were enrolled into this study. All of the patients remained seizure-free during levetiracetam treatment. Two patients died during the follow-up, and the other 13 patients were alive at the end of the study period and all were seizurefree without neurological sequelae that interfered with their daily activities. No patients experienced liver failure or rejection of the donor liver due to ineffective immunosuppressant medications. The dosage of immunosuppressants did not change before and after levetiracetam treatment, and there were no changes in hematological and biochemical data before and after treatment. Levetiracetam may be a suitable antiepileptic drug for patients who undergo liver transplantation due to fewer drug interactions and a favorable safety profile. (Medicine 94(38):e1350) Abbreviations: AED = anti-epileptic drug, LEV = Levetiracetam.
doi:10.1097/md.0000000000001350 pmid:26402799 pmcid:PMC4635739 fatcat:u5hx5hopt5fktcx5zpw6xbulyu

Factor VII-Induced MicroRNA-135a Inhibits Autophagy and Is Associated with Poor Prognosis in Hepatocellular Carcinoma

Kuang-Tzu Huang, I-Ying Kuo, Ming-Chao Tsai, Chun-Hsien Wu, Li-Wen Hsu, Li-Yu Chen, Chao-Pin Kung, Yu-Fan Cheng, Shigeru Goto, Yu-Wei Chou, Chao-Long Chen, Chih-Che Lin (+1 others)
2017 Molecular Therapy: Nucleic Acids  
Hepatocellular carcinoma (HCC) is one of the most common and aggressive malignancies worldwide. Treatment outcomes remain poor mainly due to lack of good diagnostic/prognostic markers and limited therapeutic strategies. We previously characterized aberrant activation of the TF/FVII/PAR2 pathway, which subsequently results in decreased autophagy, as a crucial event in malignant progression of HCC. Here, we identified miR-135a as a highly upregulated miRNA in HCC in response to TF/FVII/PAR2
more » ... tion. Analyzing 103 HCC patient specimens, we confirmed that miR-135a was frequently elevated in HCC tissues with higher FVII expression compared to adjacent non-cancerous counterparts. Increased miR-135a levels in HCC were also associated with tumor staging, recurrence, microvascular invasion, and decreased disease-free survival. We subsequently identified Atg14, a key component that regulates the formation of autophagosome as a direct target of miR-135a. Ectopic expression of miR-135a suppressed Atg14 levels and inhibited the autophagic processes. Our results indicate strong positive correlations between miR-135a levels and malignant behaviors in HCC patients and also suggest novel functions of miR-135a in regulation of autophagy, which could be useful as a potential target for prognostic and therapeutic uses.
doi:10.1016/j.omtn.2017.10.002 pmid:29246306 pmcid:PMC5675721 fatcat:nrms6hguwbgdvpxcflggx4essu

Analysis of Volatile Constituents in Platostoma palustre (Blume) Using Headspace Solid-Phase Microextraction and Simultaneous Distillation-Extraction

Kung, Chen, Chao, Wu, Lin, Chen
2019 Foods  
Hsian-tsao (Platostoma palustre Blume) is a traditional Taiwanese food. It is admired by many consumers, especially in summer, because of its aroma and taste. This study reports the analysis of the volatile components present in eight varieties of Hsian-tsao using headspace solid-phase microextraction (HS-SPME) and simultaneous distillation-extraction (SDE) coupled with gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). HS-SPME is a non-heating method, and the results
more » ... relatively true values of the samples during flavor isolation. However, it is a kind of headspace analysis that has the disadvantage of a lower detection ability to relatively higher molecular weight compounds; also, the data are not quantitative, but instead are used for comparison. The SDE method uses distillation 2 h for flavor isolation; therefore, it quantitatively identifies more volatile compounds in the samples while the samples withstand heating. Both methods were used in this study to investigate information about the samples. The results showed that Nongshi No. 1 had the highest total quantity of volatile components using HS-SPME, whereas SDE indicated that Taoyuan Mesona 1301 (TYM1301) had the highest volatile concentration. Using the two extraction methods, 120 volatile components were identified. Fifty-six volatile components were identified using HS-SPME, and the main volatile compounds were α-pinene, β-pinene, and limonene. A total of 108 volatile components were identified using SDE, and the main volatile compounds were α-bisabolol, β-caryophyllene, and caryophyllene oxide. Compared with SDE, HS-SPME sampling extracted a significantly higher amount of monoterpenes and had a poorer detection of less volatile compounds, such as sesquiterpenes, terpene alcohols, and terpene oxide.
doi:10.3390/foods8090415 pmid:31540084 pmcid:PMC6770287 fatcat:add25vzduvcfzpre7ycvwgjhsi

Acute melioid community-acquired pneumonia

Chia-Te Kung, Chao-Jui Li, Shin-Chiang Hung, Sheung-Fat Ko, Min-Chi Chen, Chen-Hsiang Lee, Jien-Wei Liu
2011 International Journal of Infectious Diseases  
Kung et al. / International Journal of Infectious Diseases 15 (2011) e627-e630 e630  ... 
doi:10.1016/j.ijid.2011.04.015 pmid:21696989 fatcat:2edjgq4kz5gcvnxivcokz5ozha

Feature-Rich Magnetic Quantization in Sliding Bilayer Graphenes

Yao-Kung Huang, Szu-Chao Chen, Yen-Hung Ho, Chiun-Yan Lin, Ming-Fa Lin
2014 Scientific Reports  
The generalized tight-binding model, based on the subenvelope functions of distinct sublattices, is developed to investigate the magnetic quantization in sliding bilayer graphenes. The relative shift of two graphene layers induces a dramatic transformation between the Dirac-cone structure and the parabolic band structure, and thus leads to drastic changes of Landau levels (LLs) in the spatial symmetry, initial formation energy, intergroup anti-crossing, state degeneracy and semiconductor-metal
more » ... ransition. There exist three kinds of LLs, i.e., well-behaved, perturbed and undefined LLs, which are characterized by a specific mode, a main mode plus side modes, and a disordered mode, respectively. Such LLs are clearly revealed in diverse magneto-optical selection rules. Specially, the undefined LLs frequently exhibit intergroup anti-crossings in the field-dependent energy spectra, and show a large number of absorption peaks without optical selection rules.
doi:10.1038/srep07509 pmid:25515085 pmcid:PMC4268647 fatcat:ephdfsxzovdf5cfstmingmjsf4

Characterization of latex allergenic components by capillary zone electrophoresis and N-terminal sequence analysis

Hung-Dar Chen, Chao-Ling Chen, Shih-Wen Huang, Hsiang-Fu Kung, Hao-Chia Chen
1998 Journal of Biomedical Science  
Sequencing was carried out according to the manufacturer's instructions using a standard program. 422 J Biomed Sci 1998;5:421-427 Chen/Chen/Huang/Kung/Chen Figure 1 shows the separation of latex proteinsin  ...  The immunoreactivity was determined using an ELISA. a Profile of IgE immunoreactivity, b Profile of IgG immunoreactivity against collected fractions, 424 J Biomed Sci 1998;5:421-427 CherdChen/Huang/Kung  ... 
doi:10.1007/bf02255930 pmid:9845845 fatcat:2sxte7sczjcjndbvsizvonoluy

Identification of a two-layer regulatory network of proliferation-related microRNAs in hepatoma cells

Yi Huang, Hua-Chien Chen, Chao-Wei Chiang, Chau-Ting Yeh, Shu-Jen Chen, Chen-Kung Chou
2012 Nucleic Acids Research  
Tel: +886 3 2118800 (ext. 3730); Fax: +886 3 2118800 (ext. 3533); Email: sjchen@mail.cgu.edu.tw Correspondence may also be addressed to Chen-Kung Chou.  ...  Tel: +886 3 2118800 (ext. 3354); Fax: +886 3 2118800 (ext. 3533); Email: ckchou@mail.cgu.edu.tw Present address: Chao-Wei Chiang, Institute of Biomedical Sciences, Academia Sinica, 128 Section 2 Academia  ... 
doi:10.1093/nar/gks789 pmid:22923518 pmcid:PMC3488236 fatcat:h3ldpwmarvcn3oowpykucucwfe

Diaphyseal Locking Hip Arthroplasty for Treatment of Failed Fixation of Intertrochanteric Hip Fractures

Yi-Te Chen, Wei-Ming Chen, Kung-Sheng Lee, Ching-Kuei Huang, Chao-Ching Chiang, Tain-Hsiung Chen
2008 Journal of Arthroplasty  
The Journal of Arthroplasty Vol. 23 No. 2 2008 Diaphyseal Locking Hip Arthroplasty for Treatment of Failed Fixation of Intertrochanteric Hip Fractures Yi-Te Chen, MD,* Wei-Ming Chen, MD,7 Kung-Sheng Lee  ...  , MD, PhD, 7; Ching-Kuei Huang, MD,7 Chao-Ching Chiang, MD,7; and Tain-Hsiung Chen, MDi Abstract: Between 1997 and 2004, 18 patients (8 men and 10 women, with a mean age of 73.2 years) with failed treatment  ... 
doi:10.1016/j.arth.2007.03.042 pmid:18280419 fatcat:nnq6t2c6dzd3hcqcp6zvhuk6va
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