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BloodSpot: a database of healthy and malignant haematopoiesis updated with purified and single cell mRNA sequencing profiles

Frederik Otzen Bagger, Savvas Kinalis, Nicolas Rapin
2018 Nucleic Acids Research  
BloodSpot is a gene-centric database of mRNA expression of haematopoietic cells.  ...  The visualizations have been updated to accommodate new datatypes and the database has been largely expanded with RNA-sequencing datasets, both purified in bulk and at single cell resolution, increasing  ...  The BloodSpot database update The BloodSpot webserver is updated with curated high quality RNA-sequencing data from both single cell and FACS sorted purified cells.  ... 
doi:10.1093/nar/gky1076 pmid:30395307 pmcid:PMC6323996 fatcat:4qycwaeft5f3nnpx4upqgqvhbq

Transcriptomic Analysis Identifies RNA Binding Proteins as Putative Regulators of Myelopoiesis and Leukemia

Subha Saha, Krushna Chandra Murmu, Mayukh Biswas, Sohini Chakraborty, Jhinuk Basu, Swati Madhulika, Srinivasa Prasad Kolapalli, Santosh Chauhan, Amitava Sengupta, Punit Prasad
2019 Frontiers in Oncology  
Acute myeloid leukemia (AML) is a common and aggressive hematological malignancy.  ...  to immature blast cells and a leukemic phenotype.  ...  BloodSpot: a database of healthy and malignant haematopoiesis updated with purified and single cell mRNA sequencing profiles.  ... 
doi:10.3389/fonc.2019.00692 pmid:31448224 pmcid:PMC6691814 fatcat:yezj6v4glngqjf2tj6lruowfsu

RUNX3 levels in human hematopoietic progenitors are regulated by aging and dictate erythroid-myeloid balance

Peter Balogh, Emmalee R. Adelman, John V. Pluvinage, Brian J. Capaldo, Katie C. Freeman, Sandeep Singh, Kamaleldin E. Elagib, Yukio Nakamura, Ryo Kurita, Goro Sashida, Eli R. Zunder, Hui Li (+7 others)
2019 Haematologica  
Healthy bone marrow progenitors yield a coordinated balance of hematopoietic lineages.  ...  In this study, RUNX3 was identified as a hematopoietic stem and progenitor cell factor whose levels decline with aging in humans and mice.  ...  Thank you to Joanne Lannigan, Michael Solga, Claude Chew, Alexander Wendling, and Lesa Campbell for assistance with flow cytometry  ... 
doi:10.3324/haematol.2018.208918 pmid:31171641 pmcid:PMC7109730 fatcat:nrlep46vbzeftgjefxnplyv4dq

Characterization and evolutionary origin of novel C2H2 zinc finger protein (ZNF648) required for both erythroid and megakaryocyte differentiation in humans

Daniel C. J. Ferguson, Juraidah Haji Mokim, Marjolein Meinders, Edmund R. R. Moody, Tom A. Williams, Sarah Cooke, Kongtana Trakarnsanga, Deborah E. Daniels, Ivan Ferrer-Vicens, Deborah Shoemark, Chartsiam Tipgomut, Katherine A. Macinnes (+3 others)
2020 Haematologica  
Using a combination of ZNF648 overexpression in an iPSC line and primary adult erythroid cells, ZNF648 knockdown in primary adult erythroid cells and megakaryocytes, comparative proteomics and transcriptomics  ...  Conservation of the C-terminal zinc finger domain is higher, with some variation in zinc finger number but a core of at least six zinc fingers conserved across all groups, with the N-terminus recognisably  ...  Dr Lee Carpenter, Oxford for providing the iPSCs, Profs Yukio Nakamura and Ryo Kurita, RIKEN BioResource Research Center, Japan for the HiDEP-1 cells.  ... 
doi:10.3324/haematol.2020.256347 pmid:33054117 pmcid:PMC8561289 fatcat:efxgycqkgjhghcjv2gdf2gul7a

A gain-of-function p53 mutant oncogene promotes cell fate plasticity and myeloid leukemia through the pluripotency factor Foxh1

Evangelia Loizou, Ana Banito, Geulah Livshits, Yu-Jui Ho, Richard P. Koche, Francisco J Sanchez-Rivera, Allison Mayle, Chi-Chao Chen, Savvas Kinalis, Frederik Otzen Bagger, Edward R Kastenhuber, Benjamin H. Durham (+1 others)
2019 Cancer Discovery  
We identify FOXH1 as a critical mediator of mutant p53 function that binds to and regulates stem cell-associated genes and transcriptional programs.  ...  The Trp53R172H mutation (TP53R175H in humans) exhibits a neomorphic function by promoting aberrant self-renewal in leukemic cells, a phenotype that is present in hematopoietic stem and progenitor cells  ...  BloodSpot: a database of healthy and malignant haematopoiesis updated with purified and single cell mRNA sequencing profiles. Nucleic Acids Res 2019;47(D1):D881-D5 doi 10.1093/nar/gky1076. 32.  ... 
doi:10.1158/ pmid:31068365 pmcid:PMC6606372 fatcat:etbjr4elujbrrgr2flr27qhl7q

Combinatorial molecule screening identifies a novel diterpene and the BET inhibitor CPI-203 as differentiation inducers of primary acute myeloid leukemia cells

Simon Hultmark, Aurélie Baudet, Ludwig Schmiderer, Pavan Prabhala, Sara Palma-Tortosa, Carl Sandén, Thoas Fioretos, Rajkumar Sasidharan, Christer Larsson, Sören Lehmann, Gunnar Juliusson, Fredrik Ek (+1 others)
2020 Haematologica  
, activates PKC phosphorylation and translocation of PKC to the cell membrane.  ...  First, we screened 513 natural compounds on primary AML cells and identified a novel diterpene (H4) that preferentially induced differentiation of FLT3 wild-type AMLs, while FLT3-ITD/mutations conferred  ...  Acknowledgement The authors would like to thank the staff at the Lund Stem Cell FACS Core Facilities for assistance with cell sorting. We thank Sofia Bengtsson for helping with primary samples. We  ... 
doi:10.3324/haematol.2020.249177 pmid:32855276 pmcid:PMC8485661 fatcat:xqx6gixwyjhgtcx3w3sotyg4du

PU.1 and maturational plasticity in acute myeloid leukaemia

Acute myeloid leukaemia (AML) is a haematological malignancy often framed in the context of a hierarchical leukaemia stem cell model whereby only a small proportion of immature leukaemia cells maintain  ...  These results prompt new interpretations of intratumoural phenotypic heterogeneity in AML, and may reveal novel avenues of disease relapse.  ...  Acknowledgments The authors thank Lief Carlsson (Umea University, Sweden) for providing the HPC7 cells and Andrew Wei (Australian Centre for Blood Diseases, Monash University, Australia) for providing  ... 
doi:10.26180/14401187 fatcat:xy2tbpo23rblzl3mrxc5wss75q