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BioFlow: a non-invasive, image-based method to measure speed, pressure and forces inside living cells

Aleix Boquet-Pujadas, Timothée Lecomte, Maria Manich, Roman Thibeaux, Elisabeth Labruyère, Nancy Guillén, Jean-Christophe Olivo-Marin, Alexandre C. Dufour
<span title="2017-08-23">2017</span> <i title="Springer Nature"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/tnqhc2x2aneavcd3gx5h7mswhm" style="color: black;">Scientific Reports</a> </i> &nbsp;
We illustrate the power of BioFlow in the context of amoeboid cell migration, by modelling the intracellular actin bulk flow of the parasite Entamoeba histolytica using fluid dynamics, and report unique  ...  Understanding the underlying biophysical mechanisms requires the ability to measure physical quantities inside the cell in a simple, reproducible and preferably non-invasive manner.  ...  Figure 1 depicts the biophysical quantities (here velocities, pressure, forces, and out-of-plane flow in 2D) estimated from two consecutive images in 2D and 3D (cf. Supplementary Movies S6 and S7).  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1038/s41598-017-09240-y">doi:10.1038/s41598-017-09240-y</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/28835648">pmid:28835648</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC5569094/">pmcid:PMC5569094</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/kritilk4ebe7vfqgzg2jneuh7q">fatcat:kritilk4ebe7vfqgzg2jneuh7q</a> </span>
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Zero-Mode Waveguides: A Powerful Tool for Single-Molecule Optical Studies

Zhuangxiong Huang, Serge Donkers, Nynke H. Dekker
<span title="">2011</span> <i title="Elsevier BV"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/dez2wau7t5aqvklzwrcsreuwq4" style="color: black;">Biophysical Journal</a> </i> &nbsp;
We suggest that the three-dimensional organization of islet cells and the dynamics of islet blood flow have a role in regulating insulin and glucagon secretion.  ...  Fluorescent protein (FP)-based biosensors are increasingly valuable tools for identifying subcellular dynamic processes in live cells.  ...  We suggest that the three-dimensional organization of islet cells and the dynamics of islet blood flow have a role in regulating insulin and glucagon secretion.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.bpj.2010.12.3565">doi:10.1016/j.bpj.2010.12.3565</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/t2gbceiasrekdhapedoxkvx4a4">fatcat:t2gbceiasrekdhapedoxkvx4a4</a> </span>
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Superresolution Optical Microscopy of Isolated Cardiac Mitochondrial Proteins

Harpreet Singh, Pedro Felipe, Gardeazábal Rodríguez, Rong Lu, Jean Chrisostome Bopassa, Yong Wu, Ligia Toro, Enrico Stefani
<span title="">2011</span> <i title="Elsevier BV"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/dez2wau7t5aqvklzwrcsreuwq4" style="color: black;">Biophysical Journal</a> </i> &nbsp;
We suggest that the three-dimensional organization of islet cells and the dynamics of islet blood flow have a role in regulating insulin and glucagon secretion.  ...  Fluorescent protein (FP)-based biosensors are increasingly valuable tools for identifying subcellular dynamic processes in live cells.  ...  We suggest that the three-dimensional organization of islet cells and the dynamics of islet blood flow have a role in regulating insulin and glucagon secretion.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.bpj.2010.12.3564">doi:10.1016/j.bpj.2010.12.3564</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/36kulr2om5dszckdpxld2vph4a">fatcat:36kulr2om5dszckdpxld2vph4a</a> </span>
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Real-Time Hyperspectral Imaging of Multiple Biosensors in Pancreatic Beta Cells

Amicia D. Elliott, Liang Gao, Alessandro Ustione, Tomasz S. Tkaczyk, David W. Piston
<span title="">2011</span> <i title="Elsevier BV"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/dez2wau7t5aqvklzwrcsreuwq4" style="color: black;">Biophysical Journal</a> </i> &nbsp;
We suggest that the three-dimensional organization of islet cells and the dynamics of islet blood flow have a role in regulating insulin and glucagon secretion.  ...  Fluorescent protein (FP)-based biosensors are increasingly valuable tools for identifying subcellular dynamic processes in live cells.  ...  We suggest that the three-dimensional organization of islet cells and the dynamics of islet blood flow have a role in regulating insulin and glucagon secretion.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.bpj.2010.12.3562">doi:10.1016/j.bpj.2010.12.3562</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/fcx2hmuiungddfgatqfxhj57du">fatcat:fcx2hmuiungddfgatqfxhj57du</a> </span>
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In Vivo Fluorescence Imaging of Blood Flow in Mouse Pancreatic Islets

Kurt W. Short, W. Steve Head, Michael McCaughey, David W. Piston
<span title="">2011</span> <i title="Elsevier BV"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/dez2wau7t5aqvklzwrcsreuwq4" style="color: black;">Biophysical Journal</a> </i> &nbsp;
We suggest that the three-dimensional organization of islet cells and the dynamics of islet blood flow have a role in regulating insulin and glucagon secretion.  ...  Fluorescent protein (FP)-based biosensors are increasingly valuable tools for identifying subcellular dynamic processes in live cells.  ...  We suggest that the three-dimensional organization of islet cells and the dynamics of islet blood flow have a role in regulating insulin and glucagon secretion.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.bpj.2010.12.3563">doi:10.1016/j.bpj.2010.12.3563</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/y7htdmgnsja47bg7dp3diynkaa">fatcat:y7htdmgnsja47bg7dp3diynkaa</a> </span>
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Analyzing Aβ Aggregates with High Resolution Microscopy

Pia Zißmann, Aileen Susanne Funke, Stephanie Grabowski, Stefan Marawske, Lei Wang, Markus Richert, Ralf Kühnemuth, Eva Birkmann, Claus Seidel, Dieter Willbold
<span title="">2011</span> <i title="Elsevier BV"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/dez2wau7t5aqvklzwrcsreuwq4" style="color: black;">Biophysical Journal</a> </i> &nbsp;
We suggest that the three-dimensional organization of islet cells and the dynamics of islet blood flow have a role in regulating insulin and glucagon secretion.  ...  Fluorescent protein (FP)-based biosensors are increasingly valuable tools for identifying subcellular dynamic processes in live cells.  ...  We suggest that the three-dimensional organization of islet cells and the dynamics of islet blood flow have a role in regulating insulin and glucagon secretion.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.bpj.2010.12.3561">doi:10.1016/j.bpj.2010.12.3561</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/f7gkhafkn5g3nn4qvzezoou2hi">fatcat:f7gkhafkn5g3nn4qvzezoou2hi</a> </span>
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Quasi-3D Cytoskeletal Dynamics of Osteocytes under Fluid Flow

Andrew D. Baik, X. Lucas Lu, Jun Qiu, Bo Huo, Elizabeth M.C. Hillman, Cheng Dong, X. Edward Guo
<span title="">2010</span> <i title="Elsevier BV"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/dez2wau7t5aqvklzwrcsreuwq4" style="color: black;">Biophysical Journal</a> </i> &nbsp;
Although no volumetric dilatation of the whole cell was observed under flow, both the actin and MT networks experienced primarily tensile strains in all measured strain components.  ...  Osteocytes respond to dynamic fluid shear loading by activating various biochemical pathways, mediating a dynamic process of bone formation and resorption.  ...  This technique will prove useful for imaging more dynamic, physiological fluid flow profiles, such as oscillatory fluid flow at 1 Hz, in applications where z-stack scanning techniques are unable to accurately  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.bpj.2010.08.064">doi:10.1016/j.bpj.2010.08.064</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/21044578">pmid:21044578</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC2965954/">pmcid:PMC2965954</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/2zk3csimgbarvhjez4hea24wxq">fatcat:2zk3csimgbarvhjez4hea24wxq</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20121114213012/http://www.bioe.psu.edu/labs/Dong-Lab/demo_files/Publications/Newest%20Pubs/Baik%20et%20al%20-%20Biophys%20(2010).pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/a2/48/a248fb33e18f38646604e6e771673cc62b5acb50.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.bpj.2010.08.064"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> elsevier.com </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2965954" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

A Toolbox for Organelle Mechanobiology Research—Current Needs and Challenges

Qian Feng, Sung Sik Lee, Benoît Kornmann
<span title="2019-08-16">2019</span> <i title="MDPI AG"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/4clw6v34prcbvk2usotmbz6v3q" style="color: black;">Micromachines</a> </i> &nbsp;
In this paper, we take mitochondrial mechanobiology as an example to discuss recent advances and current technical challenges in this field.  ...  In addition, we discuss the needs for future toolbox development for mechanobiological research of intracellular organelles.  ...  Microfluidic devices with controllable fluid flow have been instrumental in studying shear stress response by cultured cells [17] [18] [19] .  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.3390/mi10080538">doi:10.3390/mi10080538</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/31426349">pmid:31426349</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC6723503/">pmcid:PMC6723503</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/tlokgshbjzfcbkgf36pvaqzspe">fatcat:tlokgshbjzfcbkgf36pvaqzspe</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20200214053938/https://res.mdpi.com/d_attachment/micromachines/micromachines-10-00538/article_deploy/micromachines-10-00538.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/3f/77/3f77c37d9f27d790b98e4b6c16bc2fbf6aef258c.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.3390/mi10080538"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> mdpi.com </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723503" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

Optofluidics: the interaction between light and flowing liquids in integrated devices

Jiaomeng Zhu, 1.Key Laboratory of Artificial Micro/Nano Structure of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, China, Xiaoqiang Zhu, Yunfeng Zuo, Xuejia Hu, Yang Shi, Li Liang, Yi Yang, 2.Shenzhen Research Institute, Wuhan University, Shenzhen 518000, China.
<span title="">2019</span> <i title="Opto-Electronic Advances"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/abe3jlnylnennn5st7pq6ln6ye" style="color: black;">Opto-Electronic Advances</a> </i> &nbsp;
The fluid flow in the on-chip devices is reconfigurable, non-uniform and usually transports substances being analyzed, offering a new idea in the accurate manipulation of lights and biochemical samples  ...  In this paper, we summarized the light modulation in heterogeneous media by unique fluid dynamic properties such as molecular diffusion, heat conduction, centrifugation effect, light-matter interaction  ...  Compared to conventional solid optical tweezers, it can be applied to capture living cells in a microfluidic channel and manipulate cells in the range of 280 μm in time.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.29026/oea.2019.190007">doi:10.29026/oea.2019.190007</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/m5fuxssob5d2tfp6ym3vzjw7nu">fatcat:m5fuxssob5d2tfp6ym3vzjw7nu</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20200321020133/http://www.oejournal.org/J/PDFFull/A191120000012.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/16/8b/168b5a01b45a8fd58222e5c83173f96d283e65f7.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.29026/oea.2019.190007"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> Publisher / doi.org </button> </a>

In Vitro Modeling of Mechanics in Cancer Metastasis

Andrea Malandrino, Roger D. Kamm, Emad Moeendarbary
<span title="">2017</span> <i title="American Chemical Society (ACS)"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/xl6o7ww4ijcdhfl7c64wvaziv4" style="color: black;">ACS Biomaterials Science &amp; Engineering</a> </i> &nbsp;
In addition to a multitude of genetic and biochemical alterations, abnormal morphological, structural, and mechanical changes in cells and their extracellular environment are key features of tumor invasion  ...  This is mainly due to lack of appropriate models and measurement tools.  ...  of live cells and extracellular environment.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1021/acsbiomaterials.7b00041">doi:10.1021/acsbiomaterials.7b00041</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/29457129">pmid:29457129</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC5811931/">pmcid:PMC5811931</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/i7afy2w2hzc3vp5ch2lws3qzny">fatcat:i7afy2w2hzc3vp5ch2lws3qzny</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20190426070322/http://discovery.ucl.ac.uk/10038264/1/Moeendarbary_acsbiomaterials.7b00041.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/ae/a0/aea0730890c917683f5cb88c0bd80110b4707df4.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1021/acsbiomaterials.7b00041"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> acs.org </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811931" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

Cell refractive index for cell biology and disease diagnosis: past, present and future

P. Y. Liu, L. K. Chin, W. Ser, H. F. Chen, C.-M. Hsieh, C.-H. Lee, K.-B. Sung, T. C. Ayi, P. H. Yap, B. Liedberg, K. Wang, T. Bourouina (+1 others)
<span title="">2016</span> <i title="Royal Society of Chemistry (RSC)"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/yyrbrlaaind6tltqhv5eznybou" style="color: black;">Lab on a Chip</a> </i> &nbsp;
Cell refractive index is an important biophysical parameter, which provides new biological and biomedical insight for disease diagnosis and cell biology.  ...  With the recent development in advanced optical imaging systems, the 2D and 3D refractive indices of a single cell down to the sub-micron resolution are now feasible and demonstrated (Fig. 1c) .  ...  More sophisticated and complex optical systems are developed to measure the 2D refractive index profile in a surface layer and, more recently, the 3D refractive index profile of a single cell.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1039/c5lc01445j">doi:10.1039/c5lc01445j</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/26732872">pmid:26732872</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/2y563imsxnbhjifnhbr7wf2p3m">fatcat:2y563imsxnbhjifnhbr7wf2p3m</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20190222051313/http://pdfs.semanticscholar.org/3432/38c190a9fed49497352f960b0169b7205284.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/34/32/343238c190a9fed49497352f960b0169b7205284.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1039/c5lc01445j"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> Publisher / doi.org </button> </a>

Microfluidic platforms for cell cultures and investigations

Maria Laura Coluccio, Gerardo Perozziello, Natalia Malara, Elvira Parrotta, Peng Zhang, Francesco Gentile, Tania Limongi, Pushparani Michael Raj, Gianni Cuda, Patrizio Candeloro, Enzo Di Fabrizio
<span title="">2019</span> <i title="Elsevier BV"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/snysah2egbecpniixhah2wpx2i" style="color: black;">Microelectronic Engineering</a> </i> &nbsp;
A B S T R A C T This review covers several aspects of microfluidic devices used for culturing and monitoring of both adherent and non-adherent cells, including a multitude of applications.  ...  Hence several examples on cell culture and monitoring are given in this review, including details on design of microfluidic devices with their distinctive technological peculiarities.  ...  GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University "Magna Graecia" of Catanzaro.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.mee.2019.01.004">doi:10.1016/j.mee.2019.01.004</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/zn4oj4bqozbt5jr3xztsxw7azu">fatcat:zn4oj4bqozbt5jr3xztsxw7azu</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20190501170707/https://repository.kaust.edu.sa/bitstream/handle/10754/631136/1-s2.0-S016793171930019X-main.pdf;jsessionid=8C97B0946640C4D10510401B3A283A35?sequence=1" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/27/0c/270c05f0834e9149ee23af03d6a014da521b84f1.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.mee.2019.01.004"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> elsevier.com </button> </a>

Measuring Absolute Cell Volume Using Quantitative-Phase Digital Holographic Microscopy and a Low-Cost, Open-Source, and 3D-Printed Flow Chamber

Erik Bélanger, Sébastien A. Lévesque, Émile Rioux-Pellerin, Pauline Lavergne, Pierre Marquet
<span title="2019-11-06">2019</span> <i title="Frontiers Media SA"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/im246jdlofanriyhr3bpyw2cwy" style="color: black;">Frontiers in Physics</a> </i> &nbsp;
Therefore, in this article we describe the development of a low-cost, open-source, and 3D-printed flow chamber optimized to perform non-invasive accurate measurements of important cell biophysical parameters  ...  The proposed flow chamber has been used to perform measurements of the whole-cell refractive index, mean cell thickness, and absolute cell volume of three cell types in culture during resting state, and  ...  , Chloé Martel, Jean-Michel Mugnes, and Jean-Xavier Giroux for their contribution in the development of the flow chamber and post-processing of quantitative-phase images.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.3389/fphy.2019.00172">doi:10.3389/fphy.2019.00172</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/l7ge5i5gqjewjpoxokavgk6tja">fatcat:l7ge5i5gqjewjpoxokavgk6tja</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20200208131109/https://fjfsdata01prod.blob.core.windows.net/articles/files/457094/pubmed-zip/.versions/1/.package-entries/fphy-07-00172/fphy-07-00172.pdf?sv=2015-12-11&amp;sr=b&amp;sig=wgur5uidjQQyAS7vkfqaa%2FwVgfmlZ3xPtBkG9bUON3g%3D&amp;se=2020-02-08T13%3A11%3A38Z&amp;sp=r&amp;rscd=attachment%3B%20filename%2A%3DUTF-8%27%27fphy-07-00172.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/59/cf/59cf3d6acff3f1642f7eea8c7dcfae417de539a8.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.3389/fphy.2019.00172"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> frontiersin.org </button> </a>

In vitro elastogenesis: instructing human vascular smooth muscle cells to generate an elastic fiber-containing extracellular matrix scaffold

Svenja Hinderer, Nian Shen, Léa-Jeanne Ringuette, Jan Hansmann, Dieter P Reinhardt, Sara Y Brucker, Elaine C Davis, Katja Schenke-Layland
<span title="2015-03-18">2015</span> <i title="IOP Publishing"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/7hkj6uuadbhzdj7pxp4pw22gqi" style="color: black;">Biomedical Materials</a> </i> &nbsp;
In the present study, we seeded primary isolated human vascular smooth muscle cells (VSMCs) onto 3D electrospun scaffolds and exposed them to defined laminar shear stress using a customized bioreactor  ...  To conclude, the combination of a novel dynamic flow bioreactor and an electrospun hybrid polymer scaffold allowed the production and assembly of an elastic fiber-containing ECM.  ...  Acknowledgments The authors thank A Kahlig (IGVP, University Stuttgart), S Geist and A-L Tremmer (both Dept. of Women's Health, Eberhard Karls University  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1088/1748-6041/10/3/034102">doi:10.1088/1748-6041/10/3/034102</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/25784676">pmid:25784676</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/tcv54i53v5dnhk425fj7hwjjqu">fatcat:tcv54i53v5dnhk425fj7hwjjqu</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20210715101140/https://escholarship.mcgill.ca/downloads/dv13zz70z" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/30/e2/30e23f560339c4e569a7509043b3a55425190c24.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1088/1748-6041/10/3/034102"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> iop.org </button> </a>

Rapid 3D Refractive-Index Imaging of Live Cells in Suspension without Labeling Using Dielectrophoretic Cell Rotation

Mor Habaza, Michael Kirschbaum, Christian Guernth-Marschner, Gili Dardikman, Itay Barnea, Rafi Korenstein, Claus Duschl, Natan T. Shaked
<span title="2016-10-21">2016</span> <i title="Wiley"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/ifj4v2fyuvegzgqkhtt4dthbna" style="color: black;">Advanced Science</a> </i> &nbsp;
Alternatively, the cells can be A major challenge in the field of optical imaging of live cells is achieving rapid, 3D, and noninvasive imaging of isolated cells without labeling.  ...  cells are 3D dynamic microscopic objects that constantly adjust their sizes, shapes, and other biophysical features.  ...  DOI: 10.1002/advs.201600205 cells are 3D dynamic microscopic objects that constantly adjust their sizes, shapes, and other biophysical features.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1002/advs.201600205">doi:10.1002/advs.201600205</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/28251046">pmid:28251046</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC5323858/">pmcid:PMC5323858</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/td2re5t5hbd5lk2vgqehmgv5ii">fatcat:td2re5t5hbd5lk2vgqehmgv5ii</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20200211172537/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5323858&amp;blobtype=pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/db/8f/db8f54c5ff3522de933dca3c177ba6a89ef568e6.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1002/advs.201600205"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> wiley.com </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5323858" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>
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