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The plant cytoskeleton: a key tool for agro-biotechnology

Nigel Chaffey
2009 Annals of Botany  
g-tubulins and intermediate filaments only -such a reduced list of cytoskeletal components that the term 'fundamentals' seems difficult to justify); (2) cytoskeleton and development (featuring one of  ...  s The plant cytoskeleton: a key tool for agro-biotechnology (abbreviated hereafter to TPC).  ... 
doi:10.1093/aob/mcp084 pmcid:PMC2685313 fatcat:vpbdtzijazaopivgyv6bdgewsy

Identification of a Novel Keratin 9 Missense Mutation in a Chinese Family with Epidermolytic Palmoplantar Keratoderma

Heng Xiao, Yi Guo, Junhui Yi, Hong Xia, Hongbo Xu, Lamei Yuan, Pengzhi Hu, Zhijian Yang, Zhenghao He, Hongwei Lu, Hao Deng
2018 Cellular Physiology and Biochemistry  
We wish to thank our clinical colleagues for their efforts in collecting clinical and genetic data.  ...  Acknowledgements We are grateful to the proband and his family; without their cooperation and contribution, this work would not have been performed.  ...  Bioinformatics tools predicted that the variant was "disease-causing".  ... 
doi:10.1159/000489381 pmid:29719290 fatcat:qnlbmoipybeyzbglgqtrd5xbxi

Integrating mass spectrometry and genomics for cyanobacterial metabolite discovery

Nathan A. Moss, Matthew J. Bertin, Karin Kleigrewe, Tiago F. Leão, Lena Gerwick, William H. Gerwick
2015 Journal of Industrial Microbiology & Biotechnology  
this discussion the use of genomics, mass spectrometric networking, biochemical characterization, and isolation and structure elucidation techniques.  ...  , mass spectrometry, and biochemical studies has provided effective tools for understanding their fundamental pathways.  ...  While the development of more robust heterologous expression tools would usher a transformative approach to studying cyanobacterial natural products, it is clear that the combination of genome analysis  ... 
doi:10.1007/s10295-015-1705-7 pmid:26578313 pmcid:PMC5065021 fatcat:kec4w2pqbvfkzhaqpuffntj3pm

Bioinformatics analysis of genes associated with the patchy-type alopecia areata: CD2 may be a new therapeutic target

Jiaxiao Shi, Peng Peng, Weixiao Liu, Peng Mi, Cong Xing, Guangzhi Ning, Shiqing Feng
2019 Biomedical Papers of the Faculty of Medicine of Palacky University  
The up-regulated DEGs were related to the immune response and chemokine signaling pathway. Meanwhile, down-regulated DEGs were enriched in keratin filament and intermediate filament.  ...  Further study is needed to explore the value of CD2 in the treatment of alopecia areata.  ...  and intermediate filament.  ... 
doi:10.5507/bp.2019.049 pmid:31558844 fatcat:frg6o5733fgq3haa7i7kxmm7l4

Cell structure and dynamics

Anthony A Hyman, Jonathon Howard
2005 Current Opinion in Cell Biology  
proteins, also contains relatives of intermediate filaments.  ...  of structural and single-molecule studies.  ... 
doi:10.1016/j.ceb.2004.12.013 fatcat:6b2x3vgbtzcetkdpbf2duesyca

Electron Cryomicroscopy of Biological Machines at Subnanometer Resolution

Wah Chiu, Matthew L. Baker, Wen Jiang, Matthew Dougherty, Michael F. Schmid
2005 Structure  
Bridging the information gap: computational tools for intermediate resolution structure interpretation. 10, 131-135. King, J., and Chiu, W. (1997).  ...  the crystal structures of the individual components. two distinct types of machines amenable to cryo-EM However, cryo-EM can be the sole structural tool in the investigations at subnanometer resolutions  ...  Acknowledgments groundwork for using cryo-EM density maps to build and assess homology models based on low sequence  ... 
doi:10.1016/j.str.2004.12.016 pmid:15766537 fatcat:7q33fiifdvevxohmpkmey2om4m

A Missense Mutation within the Helix Termination Motif of KRT25 Causes Autosomal Dominant Woolly Hair/Hypotrichosis

Xia Yu, Fuyin Chen, Cheng Ni, Guolong Zhang, Luyao Zheng, Jia Zhang, Chengrang Li, Aileen Sandilands, Zhirong Yao, Ming Li
2018 Journal of Investigative Dermatology  
ACKNOWLEDGMENTS We thank all patients and their families for their ongoing participation in this study. We thank Dr. Yutaka Shimomura for providing the pCXN2.1-Wt-K25-Flag expression vector.  ...  Leu residue at position 376 is In order to predict the potential effect of p.Leu376Arg variant on the function of human K25,we integrated multiple bioinformatics tools.  ...  filament IF: intermediate filaments HS: hair shaft ).  ... 
doi:10.1016/j.jid.2017.08.035 pmid:28899683 fatcat:2xwdwzmo6zadxemmvuyaoly6dm

Expression of Vimentin Intermediate Filament for Vascular Development in Olive Flounder (Paralichthys olivaceus)

Hyun Yang, Jang-Wook Lee, Jae Koo Noh, Hyun Chul Kim, Choul-Ji Park, Jong-Won Park, In Joon Hwang, Sung Yeon Kim, Jeong-Ho Lee
2014 Development & Reproduction  
In addition, in the myoplasm, intermediate filaments connect to dense bodies to which actin filaments also attach (Fuchs & Cleveland, 1998; Prahlad et al., 1998) .  ...  filaments in flatfish developmental stages has not been sufficiently studied.  ... 
doi:10.12717/dr.2014.18.2.107 pmid:25949178 pmcid:PMC4282252 fatcat:2f5lib42dvbofkx6uuul7ghbcq

Capturing Natural Product Biosynthetic Pathways from Uncultivated Symbiotic Bacteria of Marine Sponges Through Metagenome Mining: A Mini-Review

Agustinus Robert Uria
2015 Squalen  
Metagenome mining is a powerful cultivation-independent tool that can be used to search for new natural product biosynthetic pathways from highly complex bacterial consortia.  ...  be predicted with high accuracy through bioinformatic analysis and sometimes combined with functional proof.  ...  Structural studies of AT's binding pocket have been used to create predictive tools for the specificity of malonyl-CoA (MCoA), methylmalonyl-CoA (MMCoA), and other less common substrates (Anand et al.  ... 
doi:10.15578/squalen.v10i1.131 fatcat:aplgdsquhjcd5jg7jqh7x55qqy

Ultrastructural Organization of Budding Yeast Septin Filaments Both in vitro and in situ, Analyzed by Electron Microscopy

Aurelie Bertin, Michael McMurray, Luong Thai, Galo Garcia, Jeremy Thorner, Eva Nogales
2010 Biophysical Journal  
Here, we used electron cryo-microscopy (Cryo-EM) and image analysis to study the structures of LDL and LDLLDLr complex.  ...  We are performing structural studies on purified DHPRs and RyRs to understand structural details involved in the gating mechanism. The DHPR is a heteropentamer with total molecular weight of~450 kDa.  ...  Here, we used electron cryo-microscopy (Cryo-EM) and image analysis to study the structures of LDL and LDLLDLr complex.  ... 
doi:10.1016/j.bpj.2009.12.2069 fatcat:dhksnrarindnhbvzhkwzaftlyi

Novel origin of lamin-derived cytoplasmic intermediate filaments in tardigrades

Lars Hering, Jamal-Eddine Bouameur, Julian Reichelt, Thomas M Magin, Georg Mayer
2016 eLife  
Intermediate filament (IF) proteins, including nuclear lamins and cytoplasmic IF proteins, are essential cytoskeletal components of bilaterian cells.  ...  These results suggest that a lamin derivative has been co-opted to enhance tissue stability in tardigrades, a function otherwise served by cytoplasmic IF proteins in all other bilaterians.  ...  Intermediate 497 filament structure: the bottom-up approach. Curr. Opin. Cell. Biol. 32, 65-72. , H., and Aebi, U. (2004).  ... 
doi:10.7554/elife.11117 pmid:26840051 pmcid:PMC4829535 fatcat:rdpjuc2ogvgfnhoj4rzl7jftjm

mycelyso – high-throughput analysis of Streptomyces mycelium live cell imaging data

Christian Carsten Sachs, Joachim Koepff, Wolfgang Wiechert, Alexander Grünberger, Katharina Nöh
2019 BMC Bioinformatics  
Results: We present the tool mycelyso (MYCElium anaLYsis SOftware), an image analysis system tailored to fully automated hyphae-level processing of image stacks generated by time-lapse microscopy.  ...  Streptomycetes are filamentous microorganisms of high biotechnological relevance, especially for the production of antibiotics.  ...  Graph data structures and intermediate segmentation results are visualized for checking the processed results.  ... 
doi:10.1186/s12859-019-3004-1 fatcat:4lvgiqsg7jesfo3gkut7xkmrgq

Simultaneous Multicomponent Registration of High-Resolution X-Ray Structures into Electron Microscopy Maps

Mirabela Rusu, Stefan Birmanns
2010 Biophysical Journal  
Here, we used electron cryo-microscopy (Cryo-EM) and image analysis to study the structures of LDL and LDLLDLr complex.  ...  We are performing structural studies on purified DHPRs and RyRs to understand structural details involved in the gating mechanism. The DHPR is a heteropentamer with total molecular weight of~450 kDa.  ...  Here, we used electron cryo-microscopy (Cryo-EM) and image analysis to study the structures of LDL and LDLLDLr complex.  ... 
doi:10.1016/j.bpj.2009.12.2066 fatcat:h5kfswthjfcf5hp3w5fltrwlfq

The Desmin (DES) Mutation p.A337P Is Associated with Left-Ventricular Non-Compaction Cardiomyopathy

Olga Kulikova, Andreas Brodehl, Anna Kiseleva, Roman Myasnikov, Alexey Meshkov, Caroline Stanasiuk, Anna Gärtner, Mikhail Divashuk, Evgeniia Sotnikova, Sergey Koretskiy, Maria Kharlap, Viktoria Kozlova (+6 others)
2021 Genes  
DES encodes the muscle-specific intermediate filament protein desmin and DSP encodes desmoplakin, which is a cytolinker protein connecting desmosomes with the intermediate filaments.  ...  Because the majority of DES mutations cause severe filament assembly defects and because this mutation was found in both affected patients, we analyzed this DES mutation in vitro by cell transfection experiments  ...  Several bioinformatic prediction tools suggested a high probability for a damaging effect of DES-p.A337P (Table 2 ).  ... 
doi:10.3390/genes12010121 pmid:33478057 fatcat:dyrdnaftkbe5hktftkq3rrgfju

Calcium Gating by the DHPR-RyR1 Pair

Montserrat Samso
2010 Biophysical Journal  
Here, we used electron cryo-microscopy (Cryo-EM) and image analysis to study the structures of LDL and LDLLDLr complex.  ...  We are performing structural studies on purified DHPRs and RyRs to understand structural details involved in the gating mechanism. The DHPR is a heteropentamer with total molecular weight of~450 kDa.  ...  Here, we used electron cryo-microscopy (Cryo-EM) and image analysis to study the structures of LDL and LDLLDLr complex.  ... 
doi:10.1016/j.bpj.2009.12.2068 fatcat:77n5cvrjsngqtnatjem4hgjgpy
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