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BayGenomics: a resource of insertional mutations in mouse embryonic stem cells

D. Stryke
2003 Nucleic Acids Research  
The BayGenomics gene-trap resource (http:// baygenomics.ucsf.edu) provides researchers with access to thousands of mouse embryonic stem (ES) cell lines harboring characterized insertional mutations in  ...  Each cell line contains an insertional mutation in a specific gene. The identity of the gene that has been interrupted can be determined from a DNA sequence tag.  ...  ACKNOWLEDGEMENTS BayGenomics is a Program for Genomic Applications (PGA) sponsored by NHLBI to advance functional genomic research related to heart, lung, blood and sleep health and disorders.  ... 
doi:10.1093/nar/gkg064 pmid:12520002 pmcid:PMC165511 fatcat:hcmtrjlprbddngb4igbz4cbnki

The International Gene Trap Consortium Website: a portal to all publicly available gene trap cell lines in mouse

A. S. Nord
2006 Nucleic Acids Research  
Gene trapping is a method of generating murine embryonic stem (ES) cell lines containing insertional mutations in known and novel genes.  ...  A number of international groups have used this approach to create sizeable public cell line repositories available to the scientific community for the generation of mutant mouse strains.  ...  We thank Carol Bult at Mouse Genome Informatics, Deanna Church at the National Center for Biotechnology Information, and Bob Kuhn at University of California at Santa Cruz for their helpful suggestions  ... 
doi:10.1093/nar/gkj097 pmid:16381950 pmcid:PMC1347459 fatcat:os7arloebjcshlmdhcfgryejru

Mutation of Vps54 causes motor neuron disease and defective spermiogenesis in the wobbler mouse

Thomas Schmitt-John, Carsten Drepper, Anke Mußmann, Phillip Hahn, Melanie Kuhlmann, Cora Thiel, Martin Hafner, Andreas Lengeling, Peter Heimann, Julie M Jones, Miriam H Meisler, Harald Jockusch
2005 Nature Genetics  
To obtain a second mutated allele of Vps54, we searched the BayGenomics Clone library and identified a b-geo gene trap insertion in intron 4 of Vps54 in embryonic stem cell line RRI497 (Vps54 gt(pGT10)  ...  We identified the missense mutation L967Q in Vps54 in the wobbler mouse, an animal model of amyotrophic lateral sclerosis, and also characterized a lethal allele, Vps54 b-geo .  ...  To obtain a second mutated allele of Vps54, we searched the BayGenomics Clone library and identified a b-geo gene trap insertion in intron 4 of Vps54 in embryonic stem cell line RRI497 (Vps54 gt(pGT10)  ... 
doi:10.1038/ng1661 pmid:16244655 fatcat:nifykwmnpvav5kmzvyr7vtanje

Agpat6—a novel lipid biosynthetic gene required for triacylglycerol production in mammary epithelium

Anne P. Beigneux, Laurent Vergnes, Xin Qiao, Steven Quatela, Ryan Davis, Steven M. Watkins, Rosalind A. Coleman, Rosemary L. Walzem, Mark Philips, Karen Reue, Stephen G. Young
2006 Journal of Lipid Research  
In analyzing the sequence tags for mutant mouse embryonic stem (ES) cell lines in BayGenomics (a mouse gene-trapping resource)  ...  This work was supported by BayGenomics, a Program for Genomics Applications from the National Heart, Lung, and Blood Institute (UO1 HL-66621).  ...  Abstract In analyzing the sequence tags for mutant mouse embryonic stem (ES) cell lines in BayGenomics (a mouse gene-trapping resource), we identified a novel gene, 1acylglycerol-3-phosphate O-acyltransferase  ... 
doi:10.1194/jlr.m500556-jlr200 pmid:16449762 pmcid:PMC3196597 fatcat:ynh64tm4jvbprmyqgzv2btowlu

Mutant non-coding RNA resource in mouse embryonic stem cells

Jens Hansen, Harald von Melchner, Wolfgang Wurst
2021 Disease Models & Mechanisms  
ABSTRACTGene trapping is a high-throughput approach that has been used to introduce insertional mutations into the genome of mouse embryonic stem (ES) cells.  ...  It is performed with generic gene trap vectors that simultaneously mutate and report the expression of the endogenous gene at the site of insertion and provide a DNA sequence tag for the rapid identification  ...  Acknowledgements We thank all our colleagues for generating these large-scale gene trap resources.  ... 
doi:10.1242/dmm.047803 pmid:33729986 pmcid:PMC7875499 fatcat:m3zalqhb7vh2hbsw4pyizqw5aa

New genetic resources for mammalian developmental biologists

David Beier
2010 F1000 Biology Reports  
These programs are reaching fruition, and a variety of libraries of embryonic stem cells with defined mutations are now available.  ...  The utilization of homologous recombination in embryonic stem cells as a means to generate mice carrying pre-determined modifications of genomic sequences has revolutionized the study of developmental  ...  Acknowledgments This work was supported by grants from the National Institute of Child Health and Human Development (HD36404) and the National Institute of Neurological Disorders and Stroke (MH081187).  ... 
doi:10.3410/b2-72 pmid:21173845 pmcid:PMC2989628 fatcat:7vxs2vadtrgvrojtoorkjuy4re

Current Activities of CARD as an International Core Center for Mouse Resources

Naomi NAKAGATA, Ken-ichi YAMAMURA
2009 Experimental animals  
As a founding member of the Federation of International Mouse Resources, the Asian Mouse Mutagenesis and Resource Association, and the International Gene Trap Consortium, we are contributing to the promotion  ...  to educate people, and form a hub of the domestic and international networks of both mutagenesis and resource centers.  ...  In general, gene trapping is a high-throughput approach that is used to introduce insertional mutations across the genome in mouse embryonic stem (ES) cells.  ... 
doi:10.1538/expanim.58.343 pmid:19654431 fatcat:ecpeeppiizazdorrsstgbom34y

High-throughput trapping of secretory pathway genes in mouse embryonic stem cells

S. De-Zolt
2006 Nucleic Acids Research  
Gene trapping has been used by both public and private institutions to produce libraries of embryonic stem (ES) cells harboring mutations in single genes.  ...  High-throughput gene trapping is a random approach for inducing insertional mutations across the mouse genome.  ...  Conflict of interest statement. None declared.  ... 
doi:10.1093/nar/gnj026 pmid:16478711 pmcid:PMC1369290 fatcat:ixvtakqyhzerpnjpj4htecfthq

A review of current large-scale mouse knockout efforts

Chunmei Guan, Chao Ye, Xiaomei Yang, Jiangang Gao
2010 Genesis  
As a newly-developed insertional mutation system, transposons have some advantages over retrovirus in trapping genes.  ...  Gene trapping and gene targeting are two independent techniques for making knockout mice from embryonic stem (ES) cells.  ...  TIGM contracted Lexicon to create a comprehensive knockout mouse embryonic stem cell library (also called OmniBank II) containing 350,000 cell lines of the C57BL/6N mouse strain representing more than  ... 
doi:10.1002/dvg.20594 pmid:20095055 fatcat:4efql4atj5cnrgw4htpirvgedu

Role of chromodomain helicase DNA-binding protein 2 in DNA damage response signaling and tumorigenesis

P Nagarajan, T M Onami, S Rajagopalan, S Kania, R Donnell, S Venkatachalam
2009 Oncogene  
This work was supported by the University of Tennessee startup funds, PMERF seed funds, and NIH funds (00293621) to SV and funding from NIH (AI05771901) to TMO.  ...  To further study the physiological role of Chd2 in a mammalian model, we generated a Chd2 mutant mouse model using the Baygenomics gene-trap ES cell resource.  ...  One of the ES cell clones that had been characterized to have a gene trap insertion within the Chd2 gene was represented in the Baygenomics ES cell library.  ... 
doi:10.1038/onc.2008.440 pmid:19137022 pmcid:PMC2648865 fatcat:2sjjsbbnmncpdooiyk3ux7loli

Floxin, a resource for genetically engineering mouse ESCs

Veena Singla, Julie Hunkapiller, Nicole Santos, Allen D Seol, Andrew R Norman, Paul Wakenight, William C Skarnes, Jeremy F Reiter
2009 Nature Methods  
We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs).  ...  Genetic modification of mice and of embryonic stem cells (ESCs) is an important source of insight into the functions of vertebrate genes.  ...  Thorn and the University of California, San Francisco Nikon Imaging Center for assistance with confocal microscopy, and the members of the Reiter lab for critical reading.  ... 
doi:10.1038/nmeth.1406 pmid:19966808 pmcid:PMC2895430 fatcat:7lqdrr3qxrchlpz4l3wdfuy3ly

The UniTrap resource: tools for the biologist enabling optimized use of gene trap clones

G. Roma, M. Sardiello, G. Cobellis, P. Cruz, G. Lago, R. Sanges, E. Stupka
2007 Nucleic Acids Research  
We have developed a comprehensive resource devoted to biologists wanting to optimize the use of gene trap clones in their experiments.  ...  The UniTrap resource contains data relative to 9583 trapped genes, which represent 42.3% of the mouse gene content.  ...  Table 1 . 1 Gene trap projects available in UniTrap Gene trap project ES cell clones Baygenomics 14 375 Centre for Modelling Human Disease (CMHD) 13 166 Embryonic Stem Cell Database 9736 Exchangeable  ... 
doi:10.1093/nar/gkm825 pmid:17942430 pmcid:PMC2238955 fatcat:3lposw7ifbfg7kmbocj6p5zizq

Knockout of the regulatory factor X1 gene leads to early embryonic lethality

Chenzhuo Feng, Wenhao Xu, Zhiyi Zuo
2009 Biochemical and Biophysical Research Communications - BBRC  
We have used gene trap technique to disrupt the expression of RFX1 in mice.  ...  Our results indicate that RFX1 regulates expression of genes that are essential for early embryonic development/ survival and that RFX1 function can not be compensated by other RFX1 family members.  ...  Anesthesia Research Society (2007 Frontiers in Anesthesia Research Award to Z Zuo), Cleveland, OH, by a Grant-in-Aid from the American Heart Association Mid-Atlantic Affiliate (0755450U to Z Zuo), Baltimore  ... 
doi:10.1016/j.bbrc.2009.06.111 pmid:19559676 pmcid:PMC2745335 fatcat:flhzkzg7infnxadt4u7d4baq2a

A gene trap knockout of the abundant sperm tail protein, outer dense fiber 2, results in preimplantation lethality

Nicholas A. Salmon, Renee A. Reijo Pera, Eugene Yujun Xu
2006 Genesis  
We have generated Odf2 knockout mice using an Odf2 gene trapped embryonic stem cell (ESC) line.  ...  While the expression of Odf2 highlighted a potential role for this gene in male germ cell development and centrosome function, the in vivo function of Odf2 was not known.  ...  METHODS Generation of Odf2 Knockout Mice BayGenomics mouse (strain 129/a) embryonic stem cell (ESC) line RO072, carries an Odf2 allele disrupted by the insertion of a gene trap vector (pGT2Lxf). pGT2Lxf  ... 
doi:10.1002/dvg.20241 pmid:17078042 pmcid:PMC3038656 fatcat:afm2ibm3rbhc5pozl7kjwtu4xi

Characterization of two Mst1-deficient mouse models

Montserrat C. Anguera, Matthew Liu, Joseph Avruch, Jeannie T. Lee
2008 Developmental Dynamics  
We find that the discrepancy in phenotype is most likely attributable to a second insertion in the RRT293 clone. Thus, a mutation in Mst1 alone does not affect survival.  ...  MST1 has been inferred to play important roles in apoptosis and in the inhibition of proliferation in mammalian cells.  ...  RESULTS AND DISCUSSION Derivation of Mst1-null mice from ES clone RRT293 To generate a mouse lacking the Mst1 gene, we searched the BayGenomics website and identified two embryonic stem (ES) cell clones  ... 
doi:10.1002/dvdy.21764 pmid:18942145 pmcid:PMC2596583 fatcat:f3uo7taml5c67fdzrgozgr4bqu
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