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Helicobacter pylori Lipopolysaccharide as a Possible Pathogenic Factor for Gastric Carcinogenesis [chapter]

Shin-ichi Yokota, Ken-ichi Amano, Nobuhiro Fujii
2011 Gastritis and Gastric Cancer - New Insights in Gastroprotection, Diagnosis and Treatments  
Le x is also known as CD15, which is a marker for neutrophils. The existence of Lewis antigens, such as Le x , Le y , Le a and Le b , varies among H. pylori strains.  ...  as a TLR2/TLR1 complex, activated MEK-ERK MAP kinase cascade and the transcription factor NF-Y, and upregulated transcription of the TLR4 gene ( Fig. 5-B ).  ...  This book is a comprehensive overview of invited contributions on Helicobacter pylori infection in gastritis and gastric carcinogenesis.  ... 
doi:10.5772/22874 fatcat:dpigbh364bfb5f7ovp776kqziy

Development of monoclonal antibody-based immunoassays for detection of Helicobacter pylori neutrophil-activating protein

Ianko D. Iankov, Alan R. Penheiter, Stephanie K. Carlson, Evanthia Galanis
2012 JIM - Journal of Immunological Methods  
Neutrophil-activating protein (NAP) is a major virulence factor expressed by Helicobacter pylori isolates associated with severe chronic gastroduodenal inflammation and peptic ulcers.  ...  NAP is one of the main protective antigens and a target for vaccine development against Helicobacter infection.  ...  McCormick and Mayo Proteomics Research Center, Mayo Clinic, Rochester MN for the help with design, synthesis and KLH conjugation of the NAP peptides.  ... 
doi:10.1016/j.jim.2012.06.010 pmid:22750540 pmcid:PMC3691681 fatcat:c74xsmjejjbanhzyyf6xsdllfa

Bacterial Urease and its Role in Long-Lasting Human Diseases

Iwona Konieczna, Paulina Zarnowiec, Marek Kwinkowski, Beata Kolesinska, Justyna Fraczyk, Zbigniew Kaminski, Wieslaw Kaca
2012 Current protein and peptide science  
It is essential in colonization of a host organism and in maintenance of bacterial cells in tissues. Due to its enzymatic activity, urease has a toxic effect on human cells.  ...  Detection of antibodies-binding motives (epitopes) in bacterial proteins is a complex process.  ...  ACKNOWLEDGEMENTS This work was supported by grant N N304 044639 from Ministry of Sciences and High Education (Poland).  ... 
doi:10.2174/138920312804871094 pmid:23305365 pmcid:PMC3816311 fatcat:ebczcdqn2vd73m5cknlek53n3a

Helicobacter pylorivaccination: Is there a path to protection?

Florian Anderl
2014 World Journal of Gastroenterology  
Helicobacter pylori (H. pylori ) is a pathogenic, extracellular bacterium that colonizes the stomach in approximately 50% of the world population.  ...  The colonization of H. pylori leads to ulcer development in around 20% of infected patients and may progress to gastric cancer or mucosaassociated lymphoid tissue lymphoma in 1%.  ...  Li et al [74] used three T-cell epitopes of urease B, and two B-cell epitopes from urease B and HpaA that were generated by software prediction, allowing the induction of a cellular as well as a humoral  ... 
doi:10.3748/wjg.v20.i34.11939 pmid:25232229 pmcid:PMC4161780 fatcat:4o6cg6t2ynhwxfdtbbykpalvaq

Factors involved in the pathogenesis of Helicobacter pylori infection

D. Olivares, J. P. Gisbert
2006 Revista Espanola de Enfermedades Digestivas  
Madrid, Spain Another protein involved in adhesion is NAP (neutrophil-activating protein), coded for by the napA gene and isolated from the plasma membrane protein fraction of H. pylori.  ...  A study has shown that H. pylori strains expressing the VacA protein (also called Tox + ) overexpress VEGF production through MAP kinase activation, whereas Toxstrains do not (51) .  ... 
doi:10.4321/s1130-01082006000500008 fatcat:x6pggefndjglfkbbliizht6gsy

Studies on gangliosides with affinity for Helicobacter pylori: binding to natural and chemically modified structures

H. Miller-Podraza
2003 Glycobiology  
Helicobacter pylori, like many other microbes has the ability to bind to carbohydrate epitopes and several sugar sequences have been reported as active for the bacterium including some neutral, sulfated  ...  Binding experiments confirmed a preference of H. pylori for 3-linked sialic acid and penultimate 4-linked galactose.  ...  P.R. (1995) Characterization of a Helicobacter pylori neutrophil-activating protein.  ... 
doi:10.1093/glycob/cwh028 pmid:14638632 fatcat:r5scuxvz2fezfp7p3ghby4aute

Helicobacter pylori Virulence Factors—Mechanisms of Bacterial Pathogenicity in the Gastric Microenvironment

Jacek Baj, Alicja Forma, Monika Sitarz, Piero Portincasa, Gabriella Garruti, Danuta Krasowska, Ryszard Maciejewski
2020 Cells  
The pathogenesis of gastric cancer is associated with the interaction between genetic and environmental factors, among which infection by Helicobacter pylori (H. pylori) is of major importance.  ...  The knowledge concerning the mechanisms of H. pylori pathogenicity is crucial to ameliorate eradication strategies preventing the possible induction of carcinogenesis.  ...  Neutrophil-Activating Protein Neutrophil-activating protein (NAP) is an H. pylori virulence factor that stimulates neutrophil adherence to the gastric epithelial cells, promoting the production of ROS  ... 
doi:10.3390/cells10010027 pmid:33375694 fatcat:gak6skfi3zbcnng3gqcklqni6a

A Novel Design of Multi-epitope Vaccine Against Helicobacter pylori by Immunoinformatics Approach

Junfei Ma, Jingxuan Qiu, Shuying Wang, Qianyu Ji, Dongpo Xu, Haiwang Wang, Zhiguang Wu, Qing Liu
2021 International Journal of Peptide Research and Therapeutics  
The B cell epitopes and T cell epitopes from three well performed proteins UreB, PLA1, and Omp6 were assembled into six constructs of multi-epitope vaccines with random orders.  ...  In order to select the optimal constructs, the stability of the vaccine structure and the exposure of B cell epitopes on the vaccine surface were evaluated based on structure prediction and solvent accessible  ...  And the epitope mapping score EM(P) for protein P was set as 1, if the protein from H. pylori contained any of known epitopes, otherwise the EM(P) was set as 0. (2) M II (P) = N(S II ) len(P) (3) B 1 (  ... 
doi:10.1007/s10989-020-10148-x pmid:33424523 pmcid:PMC7778422 fatcat:33twnuybnbeuvevlxqt27c4epq

Th17 cells in Helicobacter pylori infection: A dichotomy of help and harm

Beverly R.E.A. Dixon, Rafat Hossain, Rachna V. Patel, Holly M. Scott Algood
2019 Infection and Immunity  
Helicobacter pylori is a Gram-negative bacterium that infects the gastric epithelia of its human host.  ...  response, regulate B cell responses, and participate in wound healing.  ...  HELICOBACTER PYLORI ANTIGENS INVOLVED IN T CELL ACTIVATION AND DIFFERENTIATION There is evidence in the literature that specific H. pylori proteins, including several virulence factors, influence the T  ... 
doi:10.1128/iai.00363-19 pmid:31427446 pmcid:PMC6803329 fatcat:6of7x4nlxzbtxpv65nu7kronw4

Immunodominant epitope-specific Th1 but not Th17 responses mediate protection against Helicobacter pylori infection following UreB vaccination of BALB/c mice

Bin Li, Li Chen, Heqiang Sun, Wuchen Yang, Jian Hu, Yafei He, Shanshan Wei, Zhuo Zhao, Jinyong Zhang, Haibo Li, Quanming Zou, Chao Wu
2015 Scientific Reports  
Urease B subunit (UreB), a conserved protein of H. pylori, is capable of inducing specific CD4 + T-cell responses and provides protection against this infection.  ...  Helicobacter pylori (H. pylori) infects more than half of the world's population, causing chronic gastritis, peptic ulcers and gastric cancer.  ...  Acknowledgments We gratefully acknowledge Professor Weisan Chen for his constructive suggestions on antigen-specific T cell culture and his critical evaluation of this manuscript.  ... 
doi:10.1038/srep14793 pmid:26434384 pmcid:PMC4593181 fatcat:nn5b25qgirec5mnt2jznuswueu

Oral Immunization with a Multivalent Epitope-Based Vaccine, Based on NAP, Urease, HSP60, and HpaA, Provides Therapeutic Effect on H. pylori Infection in Mongolian gerbils

Le Guo, Hua Yang, Feng Tang, Runting Yin, Hongpeng Liu, Xiaojuan Gong, Jun Wei, Ying Zhang, Guangxian Xu, Kunmei Liu
2017 Frontiers in Cellular and Infection Microbiology  
In this study, a multivalent epitope-based vaccine named CWAE against H. pylori urease, neutrophil-activating protein (NAP), heat shock protein 60 (HSP60) and H. pylori adhesin A (HpaA) was constructed  ...  Epitope-based vaccine is a promising strategy for therapeutic vaccination against Helicobacter pylori (H. pylori) infection.  ...  ACKNOWLEDGMENTS 2016BZ07) and Young Talents Cultivation Plan of Ningxia Medical University.  ... 
doi:10.3389/fcimb.2017.00349 pmid:28824883 pmcid:PMC5543039 fatcat:n3ykui2nljdildieiegg6urkou

Protein signature characterizing Helicobacter pylori strains of patients with autoimmune atrophic gastritis, duodenal ulcer and gastric cancer

Valli De Re, Ombretta Repetto, Stefania Zanussi, Mariateresa Casarotto, Laura Caggiari, Vincenzo Canzonieri, Renato Cannizzaro
2017 Infectious Agents and Cancer  
Helicobacter pylori (H. pylori) represents a key factor in the etiology of autoimmune atrophic gastritis (AAG), duodenal ulcer (DU) and gastric cancer (GC).  ...  In H. pylori isolated from DU-patients a significant increase in proteins with antioxidant activity emerged (AroQ, AspA, FldA, Icd, OorA and ScoB), together with a higher content of proteins counteracting  ...  Availability of data and materials The datasets generated during the current study are available from the corresponding author on reasonable request. 2 Microbiology-Immunology and Virology, IRCCS CRO  ... 
doi:10.1186/s13027-017-0133-x pmid:28465717 pmcid:PMC5408474 fatcat:sbzfmjrw6vbwjebgv2sddmsl6e

Complete genome sequence of Helicobacter pylori B128 7.13 and a single‐step method for the generation of unmarked mutations

Emma M. Dawson, Karl A. Dunne, Emily J. Richardson, Judyta Praszkier, Dana Alfawaz, Simon Woelfel, Amanda De Paoli, Mohammad Hassan, Ian R. Henderson, Richard L. Ferrero, Amanda E. Rossiter
2019 Helicobacter  
family protein of unknown function.  ...  Helicobacter pylori represents an interesting model of bacterial pathogenesis given that most infections are asymptomatic, while a minority of infections cause severe gastric disease.  ...  In pAERP2, NapA-2W1S contains a 330 bp region of homology to the DNA sequence flanking the start codon of the neutrophil-activating protein (NapA) (light grey) which is interrupted by a 42 bp sequence  ... 
doi:10.1111/hel.12587 pmid:31062466 pmcid:PMC6618122 fatcat:kxhnyfnpnffvbpkcwlnh2bgwim

3B: H. pylori: Host bacteria interactions

1996 Gut  
Conclusions: This preliminary study indicates that the putative virulence genes assessed and neutrophil activation proteins of H. pylori are not associated.  ...  Activation of neutrophil and monocyte oxidative burst responses were measured by chemiluminescence after stimulation with sonicate proteins (1-10 jig/mL) and expressed as peak cpm in relation to the activity  ...  the CoA-transferase of pig heart mitochondria (see Corthesy-Theulaz et al., abstract). 3B:56 EPITOPE MAPPING OF HSPA, THE GROES-LIKE PROTEIN OF HELICOBACTER PYLORI (HP) J.  ... 
doi:10.1136/gut.39.suppl_2.a53 fatcat:kkwa7skdgndstmccydmddpnsde

Comparative genomics and proteomics of Helicobacter mustelae, an ulcerogenic and carcinogenic gastric pathogen

Paul W O'Toole, William J Snelling, Carlos Canchaya, Brian M Forde, Kim R Hardie, Christine Josenhans, Robert LJ Graham, Geoff McMullan, Julian Parkhill, Eugenio Belda, Stephen D Bentley
2010 BMC Genomics  
There are 26 putative outer membrane proteins in H. mustelae, some of which are most similar to the Hof proteins of H. pylori.  ...  a distinct complement of virulence-related genes including a haemagglutinin/haemolysin protein, and a glycosyl transferase for producing blood group A/B on its lipopolysaccharide.  ...  Another candidate virulence/survival determinant in H. mustelae is HMU12690, which is a homologue of the H. pylori neutrophil activating protein HP0243 [72] .  ... 
doi:10.1186/1471-2164-11-164 pmid:20219135 pmcid:PMC2846917 fatcat:fwvtqumt3jdmbdx6i2tpch4uie
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