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Histone H3 Clipping is a novel signature of Human Neutrophil Extracellular Traps [article]

Dorothea Ogmore Tilley, Ulrike Abuabed, Ursula Zimny Arndt, Monika Schmid, Stefan Florian, Peter R Jungblut, Volker Brinkmann, Alf Herzig, Arturo Zychlinsky
2021 bioRxiv   pre-print
Neutrophils are critical to host defence, executing diverse strategies to perform their antimicrobial and regulatory functions. One tactic is the production of neutrophil extracellular traps (NETs).  ...  By microscopy, this antibody distinguishes NETs from chromatin in purified and mixed cell samples. It also detects NETs in tissue sections.  ...  Acknowledgements The authors would like to give special thanks to Borko Amulic and Gerben Marsman for their constructive feedback at the early stages of manuscript preparation.  ... 
doi:10.1101/2021.03.15.434949 fatcat:2sswtqd6cvewxlyuumgoyzfoau

Computational detection and quantification of human and mouse neutrophil extracellular traps in flow cytometry and confocal microscopy

Brandon G. Ginley, Tiffany Emmons, Brendon Lutnick, Constantin F. Urban, Brahm H. Segal, Pinaki Sarder
2017 Scientific Reports  
Neutrophil extracellular traps (NETs) are extracellular defense mechanisms used by neutrophils, where chromatin is expelled together with histones and granular/cytoplasmic proteins.  ...  However, the precise assessment of in vivo relevance in different disease settings has been hampered by limited tools to quantify occurrence of extracellular traps in experimental models and human samples  ...  We thank NVIDIA Corporation for the donation of the Titan X Pascal GPU used for this research (NVIDIA, Santa Clara, CA).  ... 
doi:10.1038/s41598-017-18099-y pmid:29259241 pmcid:PMC5736696 fatcat:ozqo5yvr5vg3hjbq6rnf62koaa

Coronary Neutrophil Extracellular Trap Burden and Deoxyribonuclease Activity in ST-Elevation Acute Coronary Syndrome Are Predictors of ST-Segment Resolution and Infarct SizeNovelty and Significance

Andreas Mangold, Sherin Alias, Thomas Scherz, Thomas Hofbauer, Johannes Jakowitsch, Adelheid Panzenböck, Daniel Simon, Daniela Laimer, Christine Bangert, Andreas Kammerlander, Julia Mascherbauer, Max-Paul Winter (+4 others)
2014 Circulation Research  
Macrophage clearance of neutrophil extracellular traps is a silent process.  ...  recovered during surgical thrombectomies and open repair of abdominal aortic aneurysms. 7 PMNs hold powerful effector mechanisms, particularly by the formation of neutrophil extracellular traps (NETs)  ...  -3.4]%; P=0.01; Figure 4 . 4 Quantification of neutrophil extracellular traps (NETs) in vascular thrombi.  ... 
doi:10.1161/circresaha.116.304944 pmid:25547404 fatcat:hqc74w5d4vcknd5r6r3jjxmv6m

Aluminum oxide nanowires as safe and effective adjuvants for next-generation vaccines

Rostyslav Bilyy, Solomiya Paryzhak, Kostiantyn Turcheniuk, Tetiana Dumych, Alexandre Barras, Rabah Boukherroub, Fujia Wang, Gleb Yushin, Sabine Szunerits
2019 Materials Today  
We expect the 356 ultra-low cost of the NWs' synthesis in combination with their FIG. 3 Formation of neutrophil extracellular traps (NETs): sequestering of c-Al 2 O 3 NWs and alum by aggregated NETs after  ...  shown that nanoscaffolds may be 257 able to induce activation of polymorphonuclear neutrophils to 258 form neutrophil extracellular traps [18], networks of extracellular 259 fibers produced as an immune  ... 
doi:10.1016/j.mattod.2018.10.034 fatcat:pit5sremebakpdhjimvbgjofci

Human neutrophils produce antifungal extracellular vesicles against Aspergillus fumigatus [article]

Iordana A. Shopova, Ivan Belyaev, Prasad Dasari, Susanne Jahreis, Maria C. Stroe, Zoltan Cseresnyes, Anna Medyukhina, Carl-Magnus Svensson, Thomas Krueger, Viktoria Szeifert, Sandor Nietzsche, Theresia Conrad (+6 others)
2019 bioRxiv   pre-print
In addition to various effector mechanisms, PMNs also produce extracellular vesicles (EVs). Their contribution to antifungal defense has remained unexplored.  ...  Tracking of produced afEVs indicated that they associated with fungal cells and even entered fungal hyphae, resulting in alterations in the morphology of the fungal cell wall, and imparted antifungal effects  ...  The authors declare no conflict of interest.  ... 
doi:10.1101/620294 fatcat:pl7e3arln5bgdki4tjv7zlux54

Catheterized-bladder environment induces hyphal Candida albicans formation, promoting fungal colonization and persistence [article]

Alyssa A La Bella, Marissa J. Andersen, Alex Molesan, Peter V Stuckey, Felipe H Santiago-Tirado, Ana L. Flores-Mireles
2021 bioRxiv   pre-print
resistance and complex management of CAUTI in patients that require a urinary catheter 3 .  ...  Yet, there is poor understanding of the molecular details of how C. albicans attaches, grows in the bladder, forms biofilms, survives, and persists during CAUTI 2 .  ...  Neutrophils are able to phagocytize 356 and kill yeast cells and short hyphae while large hyphae are killed by inducing neutrophil 357 extracellular traps (NETs), which releases DNA, granule enzymes, and  ... 
doi:10.1101/2021.06.01.446547 fatcat:q5cmwcc6yna4nhuwy35bkhi75q

Platelet activation and prothrombotic properties in a mouse model of peritoneal sepsis

Fanny Vardon Bounes, Vincent Mémier, Marina Marcaud, Aemilia Jacquemin, Hind Hamzeh-Cognasse, Cédric Garcia, Jennifer Series, Pierre Sié, Vincent Minville, Marie-Pierre Gratacap, Bernard Payrastre
2018 Scientific Reports  
We show in a mouse model of peritonitis, the appearance of platelet-rich thrombi in organ microvessels and organ damage.  ...  Further studies are necessary to unravel molecular mechanisms leading to this prothrombotic state of platelets in order to guide the development of future treatments of polymicrobial sepsis.  ...  Acknowledgements We thank the personnel of Anexplo animal facilities for animal handling (US006/CREFRE Inserm/UPS), the Genotoul Imaging facility (Inserm U1048), the Cytometry facility of Inserm U1048,  ... 
doi:10.1038/s41598-018-31910-8 pmid:30201980 pmcid:PMC6131186 fatcat:hzyy4jvz7jaxdngqdpyuhky2yi

Abstracts

2011 Journal of Vascular Research  
In the first case (A) the defocus position of a nanoparticle was determined by comparing an off-focus image of the nanoparticle to a previously prepared image z-stack.  ...  In the present study we used a custom made software and compared two different, point spread function based approaches for determination of z-position of fluorescent nanoparticles.  ...  Meas. 23 (2002) 365-373 This work has been undertaken by financial support of RFBR (11-01-96018) and UrB of RAS (10-1-SP-257) References Norgen L, Eur.J.Vasc.Surg 33, S1-S70 (2007) Rossi M., Carpi  ... 
doi:10.1159/000332604 fatcat:egmxzxdg6bcyhn7iuw3qmwnjmi

Proceedings of the World Molecular Imaging Congress 2021, October 5-8, 2021: General Abstracts

2022 Molecular Imaging and Biology  
The method was applied to MRI images previously processed with clinical MRI software to determine improvements in resolution and contrast to noise ratio.  ...  Body : Purpose: To demonstrate an application of fast iterative method to enhance contrast and image resolution in clinical MRI imaging.  ...  The nanocluster system with a high selectivity showed the potential for fluorescence imaging and the integrating of gold and MMAE demonstrated excellent concurrent chemotherapy-radiotherapy efficacy, which  ... 
doi:10.1007/s11307-021-01693-y pmid:34982365 pmcid:PMC8725635 fatcat:4sfb3isoyfdhfbiwxfr55gvqym

The Fifth International Meeting of ISEV, ISEV2016, Rotterdam, The Netherlands, 4 – 7 May, 2016

2016 Journal of Extracellular Vesicles  
Adhesion of tumour cells was imaged by fluorescence microscopy, and trans-peritoneal passage of tumour cells was monitored by flow cytometry.  ...  Uptake of EVs was confirmed by fluorescence microscopy.  ...  Methods: We performed biochemical and confocal imaging experiments in isogenic cell line models to investigate whether KRAS signalling controls the secretion of Argonaute 2 (Ago2) in extracellular vesicles  ... 
doi:10.3402/jev.v5.31552 pmid:27247029 pmcid:PMC4887520 fatcat:gh3lfxdcmjfblnll6opf3p44t4

The peptidoglycan-associated protein NapA plays an important role in the envelope integrity and in the pathogenesis of the lyme disease spirochete

Marisela M. Davis, Aaron M. Brock, Tanner G. DeHart, Brittany P. Boribong, Katherine Lee, Mecaila E. McClune, Yunjie Chang, Nicholas Cramer, Jun Liu, Caroline N. Jones, Brandon L. Jutras, D. Scott Samuels
2021 PLoS Pathogens  
Here, we present evidence of a peptidoglycan-associated protein (PAP) in B. burgdorferi. Using an unbiased proteomics approach, we identified Neutrophil Attracting Protein A (NapA) as a PAP.  ...  In diderms, peptidoglycan (PG) is sandwiched between the inner and outer membrane of the cell envelope.  ...  The authors are grateful to Steve Melville, members of the Jutras lab, and Kristin Rose Jutras for their thoughtful review of this manuscript.  ... 
doi:10.1371/journal.ppat.1009546 pmid:33984073 pmcid:PMC8118282 fatcat:e6ghd6s57nfs7fos23dtkscyxm

13th EBSA congress, July 24–28, 2021, Vienna, Austria

2021 European Biophysics Journal  
The rapid evolution of the sanitary conditions across the different countries in Europe, including the progressive extension of the vaccination coverage, makes the scenario somehow unpredictable, but we  ...  are hopeful of joining a good sample of European biophysics in person in Vienna.  ...  P-20 Investigating neutrophils differentiation: towards automatic screening of nuclear morphological abnormalities with Digital Holographic Microscopy Maria Augusta do R. B. F.  ... 
doi:10.1007/s00249-021-01558-w pmid:34245338 pmcid:PMC8272448 fatcat:z2svytd4kbamrmvarle4lxwtxm

D9.2 - Impedance Measurement of Wound Infection Status

A. Schröter, G. Gerlach, A. Rösen-Wolff, AMA Association For Sensor Technology E.V., Sophie-Charlotten-Str. 15, 14059 Berlin, Germany
2013
One method is to measure neutrophil-derived extracellular DNA as an immediate response of the immune system.  ...  This was verified by microscopic observation of fluorescently stained samples. A reproducible impedance signal deviation of in average 34% after lately 4 hours was observed.  ...  From sensory perspective, granulocytes offer an interesting defense mechanism, the formation of neutrophil extracellular traps (NETs).  ... 
doi:10.5162/sensor2013/d9.2 fatcat:aeb3wx7vzfb7lk4ztl2go46ype

Congress Abstracts

2018 Hämostaseologie  
The role of CLIC1 on thrombus formation in vivo was assessed by intravital fluorescence microscopy in a mouse dorsal skin fold chamber model.  ...  The subcellular localization of CLIC1 in HUVEC as well as platelets was analyzed with fluorescence microscopy following treatment with the synthetic CLIC1 inhibitor IAA94, which was also used to assess  ...  Objectives: Neutrophils can release their chromatin forming neutrophil extracellular traps (NETs).  ... 
doi:10.1055/s-0038-1635066 fatcat:fy5yilupqzerfmxf6oq6yv3oxu

Microfluidic Sample Preparation for Single Cell Analysis

Sanjin Hosic, Shashi K. Murthy, Abigail N. Koppes
2015 Analytical Chemistry  
Acknowledgments We gratefully acknowledge financial support from the National Institutes of Health under award U24 AI118665 (SKM) and startup funds from Northeastern University (ANK).  ...  In combination with fluorescent labeling strategies, fluorescence microscopy allows for intracellular investigation against a variety of targets.  ...  (A) A microscopy image showing single neuron isolation via a micromanipulator and pipette.  ... 
doi:10.1021/acs.analchem.5b04077 pmid:26567589 pmcid:PMC4809371 fatcat:hiqiqh7qwree3gj3j7ttmislke
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