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MALDI imaging mass spectrometry: Spatial molecular analysis to enable a new age of discovery

Megan M. Gessel, Jeremy L. Norris, Richard M. Caprioli
2014 Journal of Proteomics  
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) combines the sensitivity and selectivity of mass spectrometry with spatial analysis to provide a new dimension for histological  ...  As such, MALDI IMS has the capability to become a powerful new molecular technology for the biological and clinical sciences.  ...  Acknowledgments The authors wish to acknowledge the support of grants from the National Center for Research Resources (5 P41 RR031461) and the National Institute of General Medical Sciences (8 P41GM103391  ... 
doi:10.1016/j.jprot.2014.03.021 pmid:24686089 pmcid:PMC4104210 fatcat:mzlcfaee5jdujpilaoogxnl4b4

Analysis of Tissue Specimens by Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry in Biological and Clinical Research

Jeremy L. Norris, Richard M. Caprioli
2013 Chemical Reviews  
Reid Groseclose, Jamie Allen, and Erin H. Seeley for contributing data for Figures 3, 13 , and 21, respectively. The authors further acknowledge Angus C. Grey, Rosalie K.  ...  Schey, and Zsolt Ablonczy for contributing data for Figure 18 .  ...  H&E stains of the FFPE section and fresh-frozen section (a) are included as well as ion maps (bf). An (g) enlarged H/E stain and (h) ion intensity map for m/z 1267 are also included.  ... 
doi:10.1021/cr3004295 pmid:23394164 pmcid:PMC3624074 fatcat:fw7gjr3oxfepnesqdovwbfdiqe

Mass Spectrometric Imaging for Biomedical Tissue Analysis

Kamila Chughtai, Ron M. A. Heeren
2010 Chemical Reviews  
In non-imaging MALDI analysis, the analyte is mixed with an excess of chemical matrix, usually a small organic acid at molar matrix to analyte ratio 10 3 -10 5 :1. 11 The role of the matrix is to absorb  ...  It is a powerful method that allows the analysis and detection of a wide range of biomolecules directly from tissue sections.  ...  400 μm on rat brain tissue. 69 A recent spray design theoretically predicts an improvement in achievable lateral resolution of 40 μm. 75 MALDI routinely provides a higher spatial resolution but requires  ... 
doi:10.1021/cr100012c pmid:20423155 pmcid:PMC2907483 fatcat:5golrroh5bc4vafbg5ce7muwmy

Mass Spectrometry Imaging and Integration with Other Imaging Modalities for Greater Molecular Understanding of Biological Tissues

Tiffany Porta Siegel, Gregory Hamm, Josephine Bunch, Jo Cappell, John S. Fletcher, Kristina Schwamborn
2018 Molecular Imaging and Biology  
IMC is a multiplexed method for tissue phenotyping, imaging signalling pathway or cell marker assessment, at sub-cellular resolution (1 μm).  ...  Significant technological and methodological advancements have addressed natural limitations of the techniques, i.e., increased spatial resolution, increased detection sensitivity especially for large  ...  We gratefully thank the European Society for Molecular Imaging (ESMI) for their support and the possibility of establishing a study group for Imaging Mass Spectrometry as a platform for scientific exchange  ... 
doi:10.1007/s11307-018-1267-y pmid:30167993 fatcat:ei3xvuzlcvhfljbxxqwq6yf6aa

On-tissue chemical derivatization in mass spectrometry imaging

Carla Harkin, Karl W Smith, Faye L Cruickshank, C Logan Mackay, Bryn Flinders, Ron M A Heeren, Tara Moore, Simon Brockbank, Diego F Cobice
2021 Mass spectrometry reviews (Print)  
Matrix-assisted laser desorption/ionization (MALDI) is at the forefront of MSI ionization due to its wide availability and increasing improvement in spatial resolution and analysis speed.  ...  Mass spectrometry imaging (MSI) combines molecular and spatial information in a valuable tool for a wide range of applications.  ...  Continuing improvements F I G U R E 3 Mass spectrometry imaging (MSI) strategy workflow for analysis of a new compound.  ... 
doi:10.1002/mas.21680 pmid:33433028 fatcat:f5cnlxokpzachgnerwq4f7ugwm

Surface analysis of lipids by mass spectrometry: More than just imaging

Shane R. Ellis, Simon H. Brown, Marc in het Panhuis, Stephen J. Blanksby, Todd W. Mitchell
2013 Progress in lipid research  
Mass spectrometry is now an indispensable tool for lipid analysis and is arguably the driving force in the renaissance of lipid research.  ...  In this review we describe the current state-of-the-art in the direct surface analysis of lipids with a focus on tissue sections, intact cells and thin-layer chromatography substrates.  ...  AP-MALDI has successfully been applied to the analysis of retinal lipids [97] , GLs [98] , plant tissue [99] , and animal tissue [96, 100] .  ... 
doi:10.1016/j.plipres.2013.04.005 pmid:23623802 fatcat:dyjhg4kchnbbhiqmel6uxeiyge

Tuesday posters

2008 Journal of the American Society for Mass Spectrometry  
Moor; Etienne Waelkens; K.U.Leuven, Leuven, Belgium TP 114 Improvement on Sensitivity of High-Spatial- Resolution Mass Imaging Based on MALDI-QIT- TOF-Type Mass Spectrometer; Osamu Furuhashi; Hideaki  ...  Spectral Data for Interactive Visualization of 2D and 3D Hyperspectral Images; Alex Henderson 1 ; Stephen E.  ... 
doi:10.1016/j.jasms.2008.04.011 fatcat:mtgzvrgltrcyrj4wgln7ie5y34

Male Infertility: Shining a Light on Lipids and Lipid-Modulating Enzymes in the Male Germline

Walters, Gadella, Sutherland, Nixon, Bromfield
2020 Journal of Clinical Medicine  
Regrettably, our current armory of ARTs does not constitute therapeutic treatments for male infertility, thus highlighting an urgent need for novel intervention strategies.  ...  Indeed, an imbalance of reactive oxygen species can precipitate a cascade of deleterious sequelae, beginning with the peroxidation of membrane lipids and culminating in cellular dysfunction and death.  ...  This was made possible through high spatial resolution MALDI protocols that can achieve a resolution of 5 μm [191] .  ... 
doi:10.3390/jcm9020327 pmid:31979378 pmcid:PMC7073900 fatcat:2dscofp27vhl3ekbgaand6bm2a

Applications of Free-Electron Lasers in the Biological and Material Sciences

Glenn S. Edwards, S James Allen, R. F. Haglund, R.J. Nemanich, B. Redlich, John D. Simon, W. C. Yang
2005 Photochemistry and Photobiology  
gas-phase spectroscopy and spectrometry, infrared matrix-assisted laser-desorptionionization and infrared matrix-assisted pulsed laser evaporation for analysis and processing of organic materials, human  ...  The ongoing development of ultraviolet and Xray FELs are discussed in terms of future opportunities for applications research.  ...  resolution is an essential capability for nanotechnology.  ... 
doi:10.1562/2004-11-08-ir-363 pmid:15755193 fatcat:uc5mbjuxdnhn5bpf3j4lm2zoaq

Engineering Micro‐Nanomaterials for Biomedical Translation

Yaping Chen, Maria Alba, Terence Tieu, Ziqiu Tong, Rajpreet Singh Minhas, David Rudd, Nicolas H. Voelcker, Anna Cifuentes-Rius, Roey Elnathan
2021 Advanced NanoBiomed Research  
We highlight the use of nanoneedle technology in precise manipulation of increasingly complex cellular processes at the cell-material interface, and their potential for major improvements for many fundamental  ...  Engineered nano-bio interfaces -driven by vertical micro-/nanoneedles, nanoparticles, organ-onchip devices, and a diversity of nano-substrates for mass spectroscopy imaging -are spurring scientific and  ...  M.A. and A.C-R. gratefully acknowledges the National Health and Medical Research Council (NHMRC) of Australia (GNT1125400, GNT1112432).  ... 
doi:10.1002/anbr.202100002 fatcat:fgmfshpxavbb7jj23zgb2ajik4

New techniques, applications and perspectives in neuropeptide research

Kellen DeLaney, Amanda R. Buchberger, Louise Atkinson, Stefan Gründer, Angela Mousley, Lingjun Li
2018 Journal of Experimental Biology  
Localization within tissues, for example, can be probed by mass spectrometry imaging, immunohistochemistry and radioimmunoassays.  ...  The areas of importance for neuropeptide studies include structure, localization within tissues, interaction with their receptors, including ion channels, and physiological function.  ...  Additionally, integrating multimodal imaging studies through MALDI-MS imaging and microscopy-based imaging could provide enhanced spatial and chemical information for neuropeptide localization.  ... 
doi:10.1242/jeb.151167 pmid:29439063 pmcid:PMC5818036 fatcat:eeyt42fz5rgrzp6qnnxpsdvyny

Simple, Scalable Proteomic Imaging for High-Dimensional Profiling of Intact Systems

Evan Murray, Jae Hun Cho, Daniel Goodwin, Taeyun Ku, Justin Swaney, Sung-Yon Kim, Heejin Choi, Young-Gyun Park, Jeong-Yoon Park, Austin Hubbert, Margaret McCue, Sara Vassallo (+5 others)
2015 Cell  
Here, we introduce a simple method that enables proteomic imaging for scalable, integrated, high-dimensional phenotyping of both animal tissues and human clinical samples.  ...  We have performed 22 rounds of labeling of a single tissue with precise co-registration of multiple datasets.  ...  ACKNOWLEDGMENTS We thank the entire Chung laboratory for support and helpful discussions.  ... 
doi:10.1016/j.cell.2015.11.025 pmid:26638076 pmcid:PMC5275966 fatcat:gomrshdzavgzxilh6ymju7uhha

SIRCOVA-OFTARED Congress Abstracts, Valencia, June 6-8, 2013

2013 Ophthalmic Research  
Conclusions: This new CAD for automated analysis of OCT images offers adequate sensitivity and specificity to distinguish normal OCT images from those showing potential macular pa-thology.  ...  Results and Conclusions: The use of stabilizers improves the protein solubility and the resolution of the two-dimensional electrophoresis.  ...  Conclusions: The OCT allows us to know the retina tissue structure with high resolution in vivo.  ... 
doi:10.1159/000351623 fatcat:sgai4bdyhrezjho3aelptiz7mi

Free-electron-laser-based biophysical and biomedical instrumentation

G. S. Edwards, R. H. Austin, F. E. Carroll, M. L. Copeland, M. E. Couprie, W. E. Gabella, R. F. Haglund, B. A. Hooper, M. S. Hutson, E. D. Jansen, K. M. Joos, D. P. Kiehart (+7 others)
2003 Review of Scientific Instruments  
FELs with high-peak and high-average power are enabling biophysical and biomedical investigations of infrared tissue ablation.  ...  Infrared FELs have been used as sources for inverse Compton scattering to produce a pulsed, tunable, monochromatic x-ray source for medical imaging and structural biology.  ...  However, further improvements of the resolution and reproducibility of the alignment system will be necessary for clinical use on a regular basis. V.  ... 
doi:10.1063/1.1584078 fatcat:l42s3ezj55d6zlza4tfenvttme

Structural and functional protein network analyses predict novel signaling functions for rhodopsin

C. Kiel, A. Vogt, A. Campagna, A. Chatr-aryamontri, M. Swiatek-de Lange, M. Beer, S. Bolz, A. F. Mack, N. Kinkl, G. Cesareni, L. Serrano, M. Ueffing
2014 Molecular Systems Biology  
an important level of regulation through small GTPases.  ...  Orchestration of signaling, photoreceptor structural integrity, and maintenance needed for mammalian vision remain enigmatic.  ...  Author contributions: CK, LS, GC, and MU designed the research. CK, LS, GC, AV, and MU wrote the paper. AV, MS, MB, SB, and AM performed experiments.  ... 
doi:10.1038/msb.2011.83 pmid:22108793 pmcid:PMC3261702 fatcat:x7wgkh54wfecjibe2hrayu2lp4
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