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Micropilot: automation of fluorescence microscopy–based imaging for systems biology
2011
Nature Methods
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Reflection-based autofocus 24 was carried out before each prescan acquisition (zoom 1.0). Communication channel with Micropilot was the Windows registry. ...
The Matrix Screener provided autofocus, multigrid scanning, and as the communication channel Transmission Control Protocol or Internet Protocol (TCP/IP)-based computer-aided microscopy (CAM), available ...
doi:10.1038/nmeth.1558
pmid:21258339
pmcid:PMC3086017
fatcat:dajzz3ptufellbgcazwmgnxzea
Total iron measurement in human serum with a novel smartphone-based assay
2020
IEEE Journal of Translational Engineering in Health and Medicine
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The sensor strip has a vertical flow design and is based on an optimized chemical reaction. ...
Methods: A novel assay equipped with an accurate, storable, and robust dry sensor strip, as well as a smartphone mount and (iPhone) app is used to measure total iron in human serum. ...
Your legacy will stay with us for ever. ...
doi:10.1109/jtehm.2020.3005308
pmid:32832281
pmcid:PMC7433848
fatcat:bzqkyhnnqnahxkrod7pmzgrnli
OVERVIEW OF DIGITAL FORENSICS ALGORITHMS IN DSLR CAMERAS
2017
The International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences
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It is considered that the study focuses on the problem of determination of unique feature of DSLR cameras based on optical subsystem artifacts and sensor noises. ...
Most research in this area based on the mention that the extraction of unique sensor trace of DSLR camera could be possible on the certain stage of the imaging process into the camera. ...
and produced us the opportunity to realize our possibilities. ...
doi:10.5194/isprs-archives-xlii-2-w4-199-2017
fatcat:3ugmycam7ncglpug5mjx4uri7q
GA-Adaptive Template Matching for Offline Shape Motion Tracking Based on Edge Detection: IAS Estimation from the SURVISHNO 2019 Challenge Video for Machine Diagnostics Purposes
2020
Algorithms
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Measurement of the IAS can be used as a source of information of the machine condition per se, or for performing angular resampling through Computed Order Tracking, a practice which is essential to highlight ...
By repeating the analysis frame after frame and focusing on the angular position of the screw head over time, the proposed algorithm can be used as an effective offline video-tachometer able to estimate ...
Feature-based methods minimize an error measure based on distances in the feature space, while direct methods minimize an error measure based on direct image information collected from all pixels in the ...
doi:10.3390/a13020033
fatcat:3x62ehlynjaynoxiaabic3rgdi
Precision Multiband Photometry with a DSLR Camera
2016
Publications of the Astronomical Society of the Pacific
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2015 pre-print arXiv:1506.03097v1 fatcat:lp4hiqrwczcxvmnbqpc3vzknju
We found that the DSLR achieves a best-case median absolute deviation (MAD) of 4.6 mmag per 180 s exposure when the DSLR color channels are combined, and 1000 stars are measured to better than 10 mmag ...
We conclude that the Canon 60D is a cheap, lightweight device capable of useful photometry in multiple colors. ...
Based on our own measurements with this camera we produced the diagram shown in Fig. 3 , describing the layout of the different regions on the chip. ...
doi:10.1088/1538-3873/128/961/035001
fatcat:e765yhdfdjblpphpibdpnbqmne
Development of miniaturized fluorimetric device for caffeine determination using a smartphone
2019
RSC Advances
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This study proposes a miniaturized fluorimetric device to determine caffeine in an energy drink and medicines by digital image using a smartphone. ...
Intra-and inter-day precision of the methods were evaluated based on the relative standard deviation (% RSD) from the calibration curve performed in triplicate. ...
information of a target analyte, in place of some high cost instrumental techniques like LC-MS/MS. 7, 8 igital image analysis methods are based on the use of a digital sensor like digital cameras, smartphones ...
doi:10.1039/c9ra06220c
pmid:35530710
pmcid:PMC9074131
fatcat:icavhedwk5fj7exf6iccx3p7wu
Three-dimensional localization microscopy in live flowing cells
2020
Zenodo
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The extraction of 3D positions from subcellular objects of interest is achieved by calibrating the depth-dependent response of the imaging system using fluorescent beads mixed with the sample buffer. ...
A similar change in the inter-loci distance was observable for cells that were fixed prior to imaging (Figure S3 , Methods). ...
(C) A histogram of localized positions in the two color channels. (D) A histogram of the inter-channel distance between localizations in each dimension. (E) A histogram of the 3D distance measured. ...
doi:10.5281/zenodo.3911511
fatcat:jup6bc2jincxtmfdnack4jz5me
A Tetrahedron-Based Heat Flux Signature for Cortical Thickness Morphometry Analysis
[chapter]
2018
Lecture Notes in Computer Science
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Layer for Semantic Segmentation 451 A Framework for Identifying Diabetic Retinopathy Based on Anti-noise Detection and Attention-based Fusion 458 Magnetic Resonance Spectroscopy Quantification using Deep ...
Multiple Instance Decisions Aggregated CNN 392 Respiratory Motion Modelling using cGANs 398 A deep learning-based method for automated performance evaluation in Transesoesophageal Echocardiography 405 ...
doi:10.1007/978-3-030-00931-1_48
pmid:30338317
pmcid:PMC6191198
fatcat:dqhvpm5xzrdqhglrfftig3qejq
High-throughput multicolor 3D localization in live cells by depth-encoding imaging flow cytometry
[article]
2019
bioRxiv
pre-print
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We demonstrate this approach for measuring fluorescently-labeled DNA in vitro and the chromosomal compaction state in large populations of live cells, collecting thousands of samples each minute. ...
By exploiting the laminar-flow profile in microfluidics, 3D positions can be extracted from cells or other objects of interest by calibrating the depth-dependent response of the imaging system using fluorescent ...
A similar change in the inter-loci distance was observable for cells that were fixed prior to imaging ( Figure S3 , Methods). ...
doi:10.1101/730101
fatcat:bbbqtdtqzrdyfoapbk36g7oq7u
Application of multispectral imaging in quantitative immunohistochemistry study of breast cancer: a comparative study
2015
Tumor Biology
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Multispectral imaging (MSI) based on imaging and spectroscopy, as relatively novel to the field of histopathology, has been used in biomedical multidisciplinary researches. ...
Additionally, the consistency test results indicated the inter-observer agreement was more robust in MS images for HER2 (inter-class correlation coefficient (ICC)=0.95, r s = 0.94), CK5/6 (ICC = 0.90, ...
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http:// creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution ...
doi:10.1007/s13277-015-4327-9
pmid:26537585
pmcid:PMC4844643
fatcat:abg2khzddza7zav7vczr44pa4m
Microscopy-based chromosome conformation capture enables simultaneous visualization of genome organization and transcription in intact organisms
[article]
2018
bioRxiv
pre-print
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This allowed us to unveil the changes in 3D chromatin organization occurring upon transcriptional activation and homologous chromosome un-pairing during the awakening of the zygotic genome in intact Drosophila ...
However, these methods cannot directly examine the interplay between transcriptional activation and chromosome architecture while maintaining spatial information. ...
Then, we used image-based cross-correlation of these pre-aligned fiducial barcode images to reach sub-pixel accuracy in the correction vector. ...
doi:10.1101/434266
fatcat:tl54iepqofafbkz3xde3kxwa4i
Single-molecule binding experiments on long time scales
2010
Review of Scientific Instruments
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Total internal reflection fluorescence microscopy is used to image the binding of labeled ligand to molecules specifically coupled to the surface of an optically transparent flow cell. ...
Total internal reflection fluorescence microscopy is used to image the binding of labeled ligand to molecules specifically coupled to the surface of an optically transparent flow cell. ...
An initial estimate of the mean residence time was based on a bulk measurement of the off rate for the labeled ligand ͑0.044 min −1 for class V͒. ...
doi:10.1063/1.3473936
pmid:20815611
fatcat:obf3oo5wmrh7dgq5a32hojpmw4
Single-cell systems biology by super-resolution imaging and combinatorial labeling
2012
Nature Methods
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We measured the mRNA levels of 32 genes simultaneously in single S. cerevisiae cells. ...
However, the number of molecular species that can be measured simultaneously is limited by the spectral separability of fluorophores. ...
We dedicate this paper to Xiaoliang Sunney Xie on the occasion of his 50 th birthday. ...
doi:10.1038/nmeth.2069
pmid:22660740
pmcid:PMC3418883
fatcat:oluhsammoza63iiijktw4i6bxi
Nanometer-accuracy distance measurements between fluorophores at the single-molecule level
2019
Proceedings of the National Academy of Sciences of the United States of America
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We anticipate that these methods will be useful for high-accuracy distance measurements of single molecules over a wide range of length scales. ...
Here, we describe methods that utilize information about localization and imaging errors to measure distances between two different color fluorophores with ∼1-nm accuracy at distances >2 nm. ...
Jonsson for the kinesin plasmids and discussions about the kinesin distance measurements. ...
doi:10.1073/pnas.1815826116
pmid:30770448
pmcid:PMC6410877
fatcat:g75onbx5xrh5vfppkweih5undi
TADs are 3D structural units of higher-order chromosome organization in Drosophila
2018
Science Advances
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Single-cell analysis revealed a consistent TAD-based physical compartmentalization of the chromatin fiber, with some degree of heterogeneity in intra-TAD conformations and in cis and trans inter-TAD contact ...
However, whether TADs are true physical units in each cell nucleus or whether they reflect statistical frequencies of measured interactions within cell populations is unclear. ...
We first used three-color FISH to measure intra-TAD (probes 2-1) versus inter-TAD (probes 2-3) 3D distances, with probes 1 and 3 being at the exact same genomic distance from probe 2. ...
doi:10.1126/sciadv.aar8082
pmid:29503869
pmcid:PMC5829972
fatcat:2u4qwgvy3nh45bhp3vazmhchni
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