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Single-cell microscopy of suspension cultures using a microfluidics-assisted cell screening platform

Burak Okumus, Charles J Baker, Juan Carlos Arias-Castro, Ghee Chuan Lai, Emanuele Leoncini, Somenath Bakshi, Scott Luro, Dirk Landgraf, Johan Paulsson
2017 Nature Protocols  
Studies that rely on fluorescent imaging of non-adherent cells that are cultured in suspension, such as Escherichia coli, are often hampered by trade-offs that have to be made between datathroughput and  ...  This protocol describes the assembly of electronic control circuitry, the construction of liquid handling components, and the creation of silicon masters used to cast the microfluidics chip (4-7 d).  ...  Juan Carlos Arias-Castro would like to thank to School of Sciences at Universidad de los Andes for a PhD support grant 2015-1.  ... 
doi:10.1038/nprot.2017.127 pmid:29266097 pmcid:PMC6059380 fatcat:ydcnytctozdmzbibjw6mobgtzq

Quantitative Control of Noise in Mammalian Gene Expression by Dynamic Histone Regulations [article]

Deng Tan, Rui Chen, Yuejian Mo, Wei Xu, Xibin Lu, Huiyu He, Shu Gu, Fan Jiang, Weimin Fan, Yili Wang, Xi Chen, Wei Huang
2020 bioRxiv   pre-print
It generates epigenetic bistability in H3K27ac, which contributes to large noise.  ...  Our findings could provide a mechanism-based approach to modulate noise in synthetic and endogenous gene expressions.  ...  Acknowledgments 763 This work was financially supported by the National Key R&D plan project 764 (2019YFA0906002), National Natural Science Foundation of China projects (31971181,  ... 
doi:10.1101/2020.12.20.423693 fatcat:nlunxpn4xbce3mhsjw37643fu4

Nomina generica conservanda et rejicienda spermatophytorum

H. W. Rickett, F. A. Stafleu
1959 Taxon  
Our sincere thanks are due to the officers and botanists of the several institutions whose facilities and cooperations we have enj oyed, particularly to the following:  ...  Durand as the type must be an error: this new species of Durand was based on a specimen consisting of a few fragments only. The generic diagnosis is based on Michaux' species.  ...  scandix based on the same type.  ... 
doi:10.2307/1217883 fatcat:velvl7te2zcqldhlp64ul2khv4

Table of Contents

2016 Molecular Endocrinology  
The bulge region contains four unpaired bases, with at least one non-canonical base pair forming between C6 and A25.  ...  An EEG machine with a 74-electrode cap that will collect electrical brain activity over the entire head.  ...  The Fifth Generation Aerial Target (5GAT) was designed to serve this purpose.  ... 
doi:10.1210/mend.2016.30.issue-5.toc fatcat:qwwl2b2jxjd2pcyn2o6nqqzrb4

Quantitative assessment of spatial sound distortion by the semi-ideal recording point of a hear-through device

Pablo Hoffmann, Flemming Christensen, Dorte Hammersho/i
2013 Journal of the Acoustical Society of America  
covers, and ball caps.  ...  An LED and ITC-1042 transducer, operated by an Arduino Uno processor board and a Hafler amplifier, were mounted on the array.  ...  The dependence of classification performance on bandwidth is less obvious; however, the aural classification technique is based on a sub-band type of processing that mimics the basilar membrane in the  ... 
doi:10.1121/1.4805375 fatcat:u4bvxh6karet3avpjdhpfwqpf4

Abstracts of the XXXVIII Congress of the Spanish Society of Physiological Sciences (SECF), 13-16 September 2016, Zaragoza, Spain

2016 Journal of physiology and biochemistry  
Throughout the different meetings, the SECF Congress has been considered an important instrument in attaining one of the main objectives of the Society, which is "to generate interest, stimulate research  ...  We hope that this Congress, like all the previous ones, will offer us the opportunity to learn all about the current research activities of our colleagues in the field of physiology, encouraging contact  ...  The course covers general concepts in research, its methods, and evidence-based medicine.  ... 
doi:10.1007/s13105-016-0508-2 pmid:27524160 fatcat:ifzgptw3nfg3pntly6jsi6b4me

E-Bike Engagement and Accessibility [article]

Jonah Shifrin, Julia Stuart, Jake Horcher, Jorge Alfaro, University, My, Steven Skerlos
2021
We began our design process by conducting research on existing designs solutions.  ...  The combination of necessary knowledge regarding gearing and transmission, mechatronics and motor control, battery charge and capacity, and force analyses, all in addition to general bike-related knowledge  ...  Thank you to our fellow classmates and the rest of the teaching staff, who constantly gave us feedback and asked truly meaningful questions that led us to look at our project from a different perspective  ... 
doi:10.7302/1179 fatcat:mmeikbs5lngmdb2utvjlxglfb4

Development and application of a transparent μECoG array and optrode microdrive for combined electrophysiology and optophysiology [article]

Marcel Brosch, Universitäts- Und Landesbibliothek Sachsen-Anhalt, Martin-Luther Universität, Frank Ohl
2022
The general limitations of chronic in vivo electrophysiology are device stability and signal stability.  ...  In this dissertation, I focus on improving mesoscopic electrocorticography (ECoG) recordings and microscopic single-unit recordings.  ...  We controlled our behavioral setup with an Arduino, running a custom code based on previous work (Micallef et al., 2017) .  ... 
doi:10.25673/86171 fatcat:lmrbcv6aljgslfd7keqhuu2aea

Effects of site geometry and ground motion intensity measures on lateral spread displacements

Michael Vernon Little, 0000-0001-6878-6757, Austin, The University Of Texas At, Ellen M. Rathje
2020
displacements and to identify topographic and geotechnical factors that have a significant influence on lateral spreading displacements.  ...  Sloping ground behind the free-face can increase the displacements by as much as 1 m in the zone 50-100 m from the free-face.  ...  Figure 4.1 shows an example of how a post-liquefaction intensity measure is calculated based on the time of liquefaction triggering for an evolutionary intensity measure, such as Arias intensity (Ia) or  ... 
doi:10.26153/tsw/10050 fatcat:qvbtzbs3r5g5rmqcg3tu5lomdi

Numerical Evaluation of Forces on Piled Bridge Foundations in Laterally Spreading Soil [article]

McGann CR, University Of Canterbury
2017
Recent research and case history data suggest that three-dimensional deformation of the approach embankment can significantly influence the loads placed on the embedded foundations during a flow failure  ...  This numerical work focuses on the development of efficient element formulations and appropriate mesh configurations to minimize computational effort, and seeks to frame the load reduction mechanisms in  ...  The passive pressure and side friction generated on the pile cap must also be considered in the analysis.  ... 
doi:10.26021/2416 fatcat:v5y6eiucajfjpgpkjbvou54x5u

O redesenho a partir de lógicas construtivas: O caso das charqueadas [chapter]

Fernando Franz Zauk, Valentina Toaldo Brum, Adriane Borda Almeida da Silva
2021 Educação Contemporânea – Volume 26 – Ensino, Pesquisa e Extensão  
We also thank the Biotechnology Graduate Program of the State University of Feira de Santana (PPGBiotec/UEFS).  ...  Development) and FAPESB (Foundation for Research Support of the State of Bahia).  ...  The synthesis of cDNA was performed and 2 specific primers were designed for the coding sequence of the cellulase.  ... 
doi:10.36229/978-65-5866-075-0.cap.11 fatcat:6hsxe4jwm5gubcmqlpd45t2xg4

Architecture, computation and nature: living and non-living natural models in computational architecture

Anca-Simona Horvath
2021 figshare.com  
or only metaphorically represented in the discourse and education on contemporary architecture.  ...  The contribution of this research is to fill in small gaps in the discourse on advanced computational architecture and its tools.  ...  From the technical aspects of scripting code to biomorphic paradigms of form and its association with genetics, biology, phylogeny and other branches of natural science, the impact of computation on the  ... 
doi:10.6084/m9.figshare.16571259.v1 fatcat:udktn5v6bvbhff7lyffq7sn3pa

Microfluidic handling of particles toward three-dimensional tissue printing and point of care diagnostics

Munaz Ahmed, University, My, Nam-Trung Nguyen
2019
The present thesis aims to develop a Lab-on-Chip (LOC) platform technology for mixing, separation, and trapping of biological samples, in particular for handling biological and non-biological particles  ...  Finally, positive magnetophoresis was utilised to implement on-chip mixing and subsequent separation of a biological sample for cancer detection.  ...  126 4.6 154 5.5 This chapter is under the process of publication as:  ... 
doi:10.25904/1912/3641 fatcat:oc6rtwinlrfbrentyeogp2nmbu

DIFUSÃO DO PARACETAMOL UTILIZANDO CÉLULA DE FRANZ [chapter]

Josiane Biasibetti, Danrley Dutra, Douglas Gross, Claudete Schneider
2021 Ciência, tecnologia e inovação: A nova produção do conhecimento 2  
Todos os manuscritos foram previamente submetidos à avaliação cega pelos pares, membros do Conselho Editorial desta Editora, tendo sido aprovados para a publicação com base em critérios de neutralidade  ...  for the infrastructure.  ...  ACKNOWLEGMENTS The authors thank FAPEAM (Fundação de Amparo à Pesquisa do Estado do Amazonas, Universal/FAPEAM n. 062.01076/2018) for the financial support, fellowships and the Analytical Center of UFAM  ... 
doi:10.22533/at.ed.08121310522 fatcat:z4gscbz3crfujhrfluwpaech3m

Quantitative assessment of spatial sound distortion by the semi-ideal recording point of a hear-through device

Pablo Hoffmann, Flemming Christensen, Dorte Hammershoi
2013 unpublished
covers, and ball caps.  ...  An LED and ITC-1042 transducer, operated by an Arduino Uno processor board and a Hafler amplifier, were mounted on the array.  ...  The dependence of classification performance on bandwidth is less obvious; however, the aural classification technique is based on a sub-band type of processing that mimics the basilar membrane in the  ... 
doi:10.1121/1.4799631 fatcat:ox2s7bsahzbhhcy663rwvw2nxy
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