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Application of Fingerprinting Techniques to Iodinated Nucleic Acids

H. D. Robertson, E. Dickson, P. Model, W. Prensky
1973 Proceedings of the National Academy of Sciences of the United States of America  
Several techniques of RNA and DNA fingerprinting and determination of sequence have been applied to nucleic acids labeled with 125I.  ...  Iodination as used here is thus a general labeling procedure, and appears principally to label cytidine residues.  ...  Perhaps in vitro labeling with 125I will be a valuable technique for identifying modified bases in such RNAs. FIG. 3 . Base composition analysis of iodinated nucleic acids.  ... 
doi:10.1073/pnas.70.11.3260 pmid:4522301 pmcid:PMC427212 fatcat:udn2koxrv5ds3eaepewgazwx74

Interaction of rabbit reticulocyte ribosomes with bacteriophage f1 mRNA and of Escherichia coli ribosomes with rabbit globin mRNA

S. Legon, P. Model, H. D. Robertson
1977 Proceedings of the National Academy of Sciences of the United States of America  
Using 32Pand 125I-labeled bacteriophage fI mRNA, we have shown that rabbit reticulocyte 80S ribosomes can protect specific sequences from pancreatic RNase digestion, including those sequences protected  ...  We have com ared the behavior of a prokaryotic mRNA in a eukaryotic riIosome binding system and of a eukaryotic mRNA in a prokaryotic ribosome binding system.  ...  Edward Pelle for his contributions to the sequence analysis of the 32P-labeled fI mRNA ribosome binding sites described in this paper and for additional expert assistance. We thank Mrs. C.  ... 
doi:10.1073/pnas.74.7.2692 pmid:331312 pmcid:PMC431248 fatcat:gsqjd2vbrjeprc6bhda3he42ou

Identification of specific fragments containing the 5′ end of poliovirus RNA after ribonuclease III digestion

T.J.R. Harris, J.J. Dunn, E. Wimmer
1978 Nucleic Acids Research  
The small protein (VPg) covalently linked to the 5' end of poliovirus Type 1 (PV-1) RNA has been labeled in vitro with 125I using the Bolton and Hunter reagent.  ...  When this 125I-labeled RNA is cleaved with RNase III at low monovalent salt concentrations, one major 125I-labeled fragment, approximately 100 nucleotides long, is produced.  ...  We are also indebted to Lou Deutsch for the photographs and Dr. L.  ... 
doi:10.1093/nar/5.11.4039 pmid:214765 pmcid:PMC342732 fatcat:hkxieorqwnhfjf7knmz3fab6v4

Chromosomal sites of integration of simian virus 40 DNA sequences mapped by in situ hybridization in two transformed hybrid cell lines

M Rabin, O C Uhlenbeck, D M Steffensen, W F Mangel
1984 Journal of Virology  
To detect the integrated viral DNA by in situ hybridization, we increased the sensitivity of the technique by using as a probe 125I-labeled simian virus 40 cRNA (2 X 10(9) to 3 X 10(9) dpm/micrograms)  ...  prepared by in vitro transcription of simian virus 40 DNA with high-specific-radioactivity [125I]CTP.  ...  Our approach to solving this technical problem was to synthesize high-specific-radioactivity [1251]CTP (2,000 Ci/mmol) and to use the [125I]CTP in an in vitro transcription reaction to prepare 125I-labeled  ... 
pmid:6319744 pmcid:PMC255485 fatcat:y5kemdzmd5gj7bptmna77ckzh4

Chromosomal sites of integration of simian virus 40 DNA sequences mapped by in situ hybridization in two transformed hybrid cell lines

M Rabin, O C Uhlenbeck, D M Steffensen, W F Mangel
1984 Journal of Virology  
To detect the integrated viral DNA by in situ hybridization, we increased the sensitivity of the technique by using as a probe 125I-labeled simian virus 40 cRNA (2 x 109 to 3 x 109 dpm/p.g) prepared by  ...  map to the same position, q31, on the long arm of human chromosome 7.  ...  Bane Estate (to W.F.M.) and by Public Health Service grants GM 25083 (to O.C.U.). GM 21123 (to D.M.S.), and CA 25633 (to W.F.M.) from the National Institutes of Health. LITERATURE CITED  ... 
doi:10.1128/jvi.49.2.445-451.1984 fatcat:fe2rkn234fd43pezwdprxhvqhe

No detectable reiteration of genes coding for mouse MOPC 41 immunoglobulin light-chain mRNA

M. G. Farace, M. F. Aellen, P. A. Briand, C. H. Faust, P. Vassalli, B. Mach
1976 Proceedings of the National Academy of Sciences of the United States of America  
RNA fractions rich in immunoglobulin light (L)chain mRNA were isolated from mouse myeloma MOPC 41 by procedures previously described, and chemically labeled with 125I.  ...  These RNA fractions were hybridized with MOPC 41 DNA under conditions of DNA excess.  ...  An alternative approach makes use of the mRNA itself.  ... 
doi:10.1073/pnas.73.3.727 pmid:815907 pmcid:PMC335991 fatcat:ucplmdbvqveltazjobkvsvsbvy

Identification of a proviral genome associated with avian myeloblastic leukemia

L. M. Souza, M. C. Komaromy, M. A. Baluda
1980 Proceedings of the National Academy of Sciences of the United States of America  
Preparation of AMV-S 125I-Labeled 70S RNA and Hybridization Conditions. The 70S viral RNA isolated from AMV-S (6) was labeled with 125I to a specific activity of 2.0 X 108 cpm/ttg as described (7) .  ...  Hybridization with poly(A)-selected AMV 125I-RNA at 0.01 ,ug/ml to Southern blots was carried out as with total AMV 125I-RNA.  ...  Preparation of AMV-S 125I-Labeled 70S RNA and Hybridization Conditions. The 70S viral RNA isolated from AMV-S (6) was labeled with 125I to a specific activity of 2.0 X 108 cpm/ttg as described (7) .  ... 
doi:10.1073/pnas.77.5.3004 pmid:6248880 pmcid:PMC349535 fatcat:qtgpww6pu5fxvohrlf6xyiei3m

Potato spindle tuber and citrus exocortis viroids undergo no major sequence changes during replication in two different hosts

E. Dickson, T. O. Diener, H. D. Robertson
1978 Proceedings of the National Academy of Sciences of the United States of America  
, and subjected to two-dimensional RNA fingerprinting analysis.  ...  We conclude that the nucleotide sequences of these viroids are principally determined by the infecting viroid and not by the host.  ...  Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable.  ... 
doi:10.1073/pnas.75.2.951 pmid:16592502 pmcid:PMC411376 fatcat:hqrzsavh2rdzbm2mknt4unc24a

Cowpea mosaic virus VPg: sequencing of radiochemically modified protein allows mapping of the gene on B RNA

P Zabel, M Moerman, G Lomonossoff, M Shanks, K Beyreuther
1984 EMBO Journal  
VPg covalently bound to viral RNA chains (VPg-RNA) was iodinated with chloramine-T and Bolton-Hunter reagent to label tyrosine and lysine residues, respectively.  ...  VPg within the 200 000 dalton (200 K) polyprotein encoded by CPMV B RNA and the prediction of its entire amino acid sequence.  ...  analysis.  ... 
pmid:16453534 pmcid:PMC557569 fatcat:jhay3b52ircwxprppr6ws63fnq

Preferential transcription of the ovalbumin gene in isolated hen oviduct nuclei by RNA polymerase B

M. C. Nguyen-Huu, A. A. Sippel, N. E. Hynes, B. Groner, G. Schutz
1978 Proceedings of the National Academy of Sciences of the United States of America  
Hg-[3H]cDNA and Hg-[32P]cDNA were characterized by hybridization to mRNAes and 125I-labeled mRNAes (125I-mRNAes).  ...  (ii) (3HJUTP was used to label the in vitro synthesized RNA.  ...  Hg-[3H]cDNA and Hg-[32P]cDNA were charac- terized by hybridization to mRNAes and 125I-labeled mRNAes (125I-mRNAes).  ... 
doi:10.1073/pnas.75.2.686 pmid:273231 pmcid:PMC411321 fatcat:u5h6jd3feffhzlzftebrrel75e

NUCLEOTIDE SEQUENCES OF HUMAN GLOBIN MESSENGER RNA

Bernard G. Forget, Charles A. Marotta, Sherman M. Weissman, Inder M. Verma, Ronald P. McCaffrey, David Baltimore
1974 Annals of the New York Academy of Sciences  
Sufficient nucleotide sequence information has been obtained from about 50% of the intermediate sized oligonucleotides (8-14 base residues long), to make possible examination of corre-  ...  The fingerprint patterns obtained from digests of purified natural human lOS globin messenger RNA, labeled in vitro with 12I1 or with [y-32P]ATP and polynucleotide kinase, were similar to that of the complementary  ...  cell culture labeling techniques, we have used a different approach to obtain 82P-labeled RNA.  ... 
doi:10.1111/j.1749-6632.1974.tb21888.x pmid:4611306 fatcat:oft5bappefgqjdlbi7wfg6sepu

Nucleotide Sequences of Human Globin Messenger RNA

C. A. Marotta, B. G. Forget, S. M. Weissman, I. M. Verma, R. P. McCaffrey, D. Baltimore
1974 Proceedings of the National Academy of Sciences of the United States of America  
Sufficient nucleotide sequence information has been obtained from about 50% of the intermediate sized oligonucleotides (8-14 base residues long), to make possible examination of corre-  ...  The fingerprint patterns obtained from digests of purified natural human lOS globin messenger RNA, labeled in vitro with 12I1 or with [y-32P]ATP and polynucleotide kinase, were similar to that of the complementary  ...  cell culture labeling techniques, we have used a different approach to obtain 82P-labeled RNA.  ... 
doi:10.1073/pnas.71.6.2300 pmid:4135409 pmcid:PMC388440 fatcat:sbzgsjn22jeznfmkhrxq4zau3e

Sequence arrangement of the rRNA genes of the dipteran Sarcophaga bullata

Cynthia K. French, D.L. Fouts, J.E. Manning
1981 Nucleic Acids Research  
Velocity sedimentation studies of RNA of Sarcophaga bullata show that the major rRNA species have sedimentation values of 26S and 18S.  ...  Analysis of the rRNA under denaturing conditions indicates that there is a hidden break centrally located in the 26S rRNA species.  ...  French in partial fulfillment of the requirements for the degree of Doctor of Philosophy, School of Biological Sciences, University of California at Irvine.  ... 
doi:10.1093/nar/9.11.2563 pmid:6269054 pmcid:PMC326872 fatcat:uaukyktas5hibbrqxy64r5mliq

Approaches to sequence analysis of125I-labeled RNA

Elizabeth Dickson, Louise K. Pape, Hugh D. Robertson
1979 Nucleic Acids Research  
A method is described for the initial steps of sequence analysis of RNase Tl-and pancreatic RNase-resistant oligonucleotides of RNA containing cytidylate residues labeled in vitro with 125i. i n many cases  ...  Additional methods including analysis of oligonucleotides following chemical blocking of uridylate residues with CMCT and analysis of products of incomplete enzymatic digestion are also discussed.  ...  ACKNOWLEDGEMENTS The authors would like to thank Edward G. Pelle and Edgar C. Lawson III for their expert technical assistance; Dr. Wolf Prensky of the Sloan Kettering  ... 
doi:10.1093/nar/6.1.91 pmid:106369 pmcid:PMC327676 fatcat:jdf3xfs2onfixl2ryj2ozi6zle

Neuronal-type alpha-bungarotoxin receptors and the alpha 5-nicotinic receptor subunit gene are expressed in neuronal and nonneuronal human cell lines

B. Chini, F. Clementi, N. Hukovic, E. Sher
1992 Proceedings of the National Academy of Sciences of the United States of America  
These receptors are immunologically related to the aBgtx receptors of unknown function described in the nervous system and in the IMR32 neuroblastoma cell line and are distinct from muscle nicotinic receptors  ...  a-Bungarotoxin (aBgtx) is a toxin known to interact with muscle nicotinic receptors and with some neuronal nicotinic receptors.  ...  Boulter for the gift of mouse a, (BMA407) and rat a5 (PC1321) cDNA probes; and Prof. H. Soreq for the IMR32 cDNA library. We are indebted to Dr. C.  ... 
doi:10.1073/pnas.89.5.1572 pmid:1542648 pmcid:PMC48494 fatcat:sselaaze7ncrdpx3ryd26eefeq
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