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OPINIONS Acetyl-coenzyme A carboxylase – an attractive enzyme for biotechnology

Jan Podkowiński, Aleksander Tworak
2011 BioTechnologia  
This reaction constitutes the first committed step of fatty acid synthesis in most organisms, while its product also serves as a universal precursor for various other high-value compounds.  ...  The importance of studies on acetyl-CoA carboxylase activity for design and development of new herbicides and antibiotics as well as for medical intervention is also highlighted.  ...  All genes were assembled in an artificial operon under bacteriophage T7 promoter.  ... 
doi:10.5114/bta.2011.46549 fatcat:6yytrcmdsrbrlntpe7ncelip2q

Expanding the genetic code

Lei Wang, Peter G. Schultz
2001 Chemical Communications  
[258] M. jannaschii TyrRS (MjTyrRS) discriminates tRNAs with C1:G72 (an eukaryotic recognition element) from those with G1:C72 (a prokaryotic recognition element), and does not aminoacylate crude tRNA  ...  Archaeal aminoacyl-tRNA synthetases are more similar to their eukaryotic than prokaryotic counterparts in terms of homology and tRNA recognition elements.  ... 
doi:10.1039/b108185n pmid:12120285 fatcat:fvymlsnij5fg5bppcwin6p664m

Emerging facets of prokaryotic glycosylation

Christina Schäffer, Paul Messner, Mecky Pohlschroder
2016 FEMS Microbiology Reviews  
Combining glycan structural information with bioinformatic, genetic, biochemical and enzymatic data has opened up an avenue for in-depth analyses of glycosylation processes as a basis for glycoengineering  ...  While it is estimated that more than two-thirds of all eukaryotic proteins would be glycosylated, no such estimate is available for prokaryotic glycoproteins, whose understanding is lagging behind, mainly  ...  Thus, this approach provides an efficient way for introducing complex N-glycans into polypeptides for the gain of novel protein properties that might be valuable for drug discovery (Lomino et al. 2013  ... 
doi:10.1093/femsre/fuw036 pmid:27566466 pmcid:PMC5266552 fatcat:36xdbfa2fbb47f3id6gjb52gfq

How the study of Listeria monocytogenes has led to new concepts in biology

Nathalie Rolhion, Pascale Cossart
2017 Future Microbiology  
21] and by an in silico-based approaches [22] .  ...  The first noncoding RNAs (ncRNAs) in L. monocytogenes were identified by coimmunoprecipitation with Hfq, a small RNA-binding protein required for the activity of small regulatory RNAs in prokaryotes [  ... 
doi:10.2217/fmb-2016-0221 pmid:28604108 fatcat:2mfxpor6lzfd5oyzpwcp35opsy

Transcriptional profile of the homologous recombination machinery and characterization of the EhRAD51 recombinase in response to DNA damage in Entamoeba histolytica

Mavil Lopez-Casamichana, Esther Orozco, Laurence A. Marchat, Cesar Lopez-Camarillo
2008 BMC Molecular Biology  
In eukaryotic and prokaryotic cells, homologous recombination is an accurate mechanism to generate genetic diversity, and it is also used to repair DNA double strand-breaks.  ...  Results: In this paper, we initiated the study of the mechanism for DNA repair by homologous recombination in the primitive eukaryote E. histolytica using UV-C (150 J/m 2 ) irradiated trophozoites.  ...  Our thanks are also to Alfredo Padilla and Sollange Archer (UACM) for their help in the artwork, as well as Dr. Rosana Arroyo for critical reading of manuscript.  ... 
doi:10.1186/1471-2199-9-35 pmid:18402694 pmcid:PMC2324109 fatcat:j62cysurwbfebiiofgjj2nmo2y

Evolutionary, physiological and biotechnological aspects of ferrochelatase and heme in higher plants

Patricia Ortega-Rodés, Bernhard Grimm, Eduardo Ortega
2014 Biotecnología Aplicada  
Heme, is a cofactor for essential proteins, it is synthesized after the insertion of Fe2+ into protoporphyrin ring by the ferrochelatase (FC) enzyme.  ...  Increasing the heme iron content in plant foods is a biotechnological challenge to improve the dietary intake of iron in the population; it is known that heme-contained iron is absorbed 5-10 times higher  ...  Acknowledgements Patricia Ortega Rodés and Eduardo Ortega Delgado express their gratitude to Jennifer Hosek, Mike Zirkel and Virginia Waddick for helping with the English language. plant, green algae,  ... 
doaj:30dc71472105416f86268358e469345c fatcat:d33q26zjx5de3bstca4p7bxxdu

Evolution of mate-finding Allee effect in prey

Luděk Berec, Veronika Bernhauerová, Barbara Boldin
2018 Journal of Theoretical Biology  
This meeting will be as well an open forum for interaction between theoreticians and empirical biologists with the main goal to enhance interdisciplinary approaches and stimulate further advances in developing  ...  This alternative metric can influence the shapes of niche response surfaces.  ...  Our novel stochastic separation theorems state that with high probability in high dimension and in an arbitrary set of random points any point can be separated by one formal neuron from the rest of the  ... 
doi:10.1016/j.jtbi.2017.12.024 pmid:29277599 fatcat:2p5kgwe6fffonblaccjhwlwvui

Virulence factors of theMycobacterium tuberculosiscomplex

Marina A. Forrellad, Laura I. Klepp, Andrea Gioffré, Julia Sabio y García, Hector R. Morbidoni, María de la Paz Santangelo, Angel A. Cataldi, Fabiana Bigi
2013 Virulence  
The aim of this review is to describe the bacterial genes/proteins that are essential for the virulence of MTBC species, and that have been demonstrated in an in vivo model of infection.  ...  Knowledge of MTBC virulence factors is essential for the development of new vaccines and drugs to help manage the disease toward an increasingly more tuberculosis-free world.  ...  Rodolfo Biekofsky for critical reading of the manuscript. This study was supported by grant NIH/NIAID 1R01AI083084-03. M.P.S., A.A.C. and F.B. are CONICET fellows.  ... 
doi:10.4161/viru.22329 pmid:23076359 pmcid:PMC3544749 fatcat:fsyjteyvyfggvdcxxqacklda4u

The Complete Genome Sequence of Cupriavidus metallidurans Strain CH34, a Master Survivalist in Harsh and Anthropogenic Environments

Paul J. Janssen, Rob Van Houdt, Hugo Moors, Pieter Monsieurs, Nicolas Morin, Arlette Michaux, Mohammed A. Benotmane, Natalie Leys, Tatiana Vallaeys, Alla Lapidus, Sébastien Monchy, Claudine Médigue (+6 others)
2010 PLoS ONE  
At least 25 loci for heavy-metal resistance (HMR) are distributed over the four replicons.  ...  The genome data show that C. metallidurans CH34 is particularly well equipped to live in extreme conditions and anthropogenic environments that are rich in metals.  ...  Rouy of Génoscope (Centre National de Séquençage, Evry, France) for implementing additional features in MaGe and their essential advice in genome annotation. We are also grateful to I. T.  ... 
doi:10.1371/journal.pone.0010433 pmid:20463976 pmcid:PMC2864759 fatcat:krys5b5lijd4bhp7wiiqcciokm


1998 Memórias do Instituto Oswaldo Cruz  
The genome of Trypanosoma cruzi contains tandemly arrayed alternating genes encoding the surface protein amastin and the G-like protein tuzin.  ...  In contrast, tuzin mRNA abundance is roughly equivalent in the different life cycle stages.  ...  Prokaryotes do not have histone proteins.  ... 
doi:10.1590/s0074-02761998000800005 fatcat:vmrrcqnekzahvep33w5agadtte

Evolution and comparative genomics of di-symbiotic systems in aphids from the Lachninae subfamily and genome reduction in Serratia symbiotica

Alejandro Manzano Marín, Jean-Christophe Simon, Gitta Szabó, Matthias Horn, Amparto Latorre Castillo
2016 Zenodo  
taken over by an alternative enzyme or it being non-essential in some endosymbiotic organisms.  ...  Eukaryotes, contrary to most prokaryotes, hold a rather limited metabolic repertoire, hence, symbiosis between these two types of organims has helped eukaryotes to widen their range of metabolic resources  ...  Dotted branches represent host alternating lineages.  ... 
doi:10.5281/zenodo.2565050 fatcat:c53crdyi3vfrtn3mw3ljvsblfi

An Approach to Understanding the Transmission of Mycobacterium leprae Using Molecular and Immunological Methods: Results from the MILEP2 Study

W. Cairns S. Smith, Christine M. Smith, Ian A. Cree, Ruprendra S. Jadhav, Murdo Macdonald, Vijay K. Edward, Linda Oskam, Stella van Beers, Paul Klatser
2004 International journal of leprosy and other mycobacterial diseases  
Here, we report two alternative approaches for the expression of immunologically reactive recombinant genuine DPPD.  ...  In the present study we used a genetic approach to demonstrate that an SNP within the promoter of the nitrate reductase gene cluster narGHJI is responsible for the different nitrate reductase activity  ...  Promoter recognition for one of these genes was confirmed by in vitro transcription analysis after purification of recombinant SigC and reconstitution of an Esigma(C) RNA polymerase holoenzyme.  ... 
doi:10.1489/0020-7349(2004)72<269:aatutt>;2 pmid:15485283 fatcat:goelml5hfjbmdee5lpzvwxdxhq

Identification and characterization of two closely related β-carbonic anhydrases from Chlamydomonas reinhardtii

Ruby A. Ynalvez, Ying Xiao, Ayana S. Ward, Khrishen Cunnusamy, James V. Moroney
2008 Physiologia Plantarum : An International Journal for Plant Biology  
The Factor Xa recognition amino acid sequence is shown in red. The Factor Xa cleavage site is denoted by an arrow.  ...  An attempt to elucidate the role of CAH7 using an RNA interference approach was unsuccessful.  ... 
doi:10.1111/j.1399-3054.2007.01043.x pmid:18405332 fatcat:sr3sks5txjexliz24fkdkuuuaq

Chemical Protection Against Ionizing Radiation

1966 Medical Journal of Australia  
damage. b. emc1Sobta1Im., An alternative mans by which hypoxia may be induced in cells or tissues is by local consumption of oxgen by chemical or biochemical reactions.  ...  which approach 0.5 and 2 M respectively (130,227,228,405].  ...  DRF -Dose Reduction Factor: Synonpmous with IMF, but specific for radioprotection. Rapid-mixing studies on the time-scale of radiation damage In cells.  ... 
doi:10.5694/j.1326-5377.1966.tb19518.x fatcat:vg3bnr7khbce5kqycnku53vyqi

Chemical Protection against Ionizing Radiation [chapter]

John C. Livesey, Donald J. Reed
1987 Advances in Radiation Biology  
damage. b. emc1Sobta1Im., An alternative mans by which hypoxia may be induced in cells or tissues is by local consumption of oxgen by chemical or biochemical reactions.  ...  which approach 0.5 and 2 M respectively (130,227,228,405].  ...  DRF -Dose Reduction Factor: Synonpmous with IMF, but specific for radioprotection. Rapid-mixing studies on the time-scale of radiation damage In cells.  ... 
doi:10.1016/b978-0-12-035413-9.50010-8 fatcat:4522xdhn7vgmnc6ocn5vezcofq
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