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Alternative Splicing in the Differentiation of Human Embryonic Stem Cells into Cardiac Precursors

Nathan Salomonis, Brandon Nelson, Karen Vranizan, Alexander R. Pico, Kristina Hanspers, Allan Kuchinsky, Linda Ta, Mark Mercola, Bruce R. Conklin, Manuel Ares
2009 PLoS Computational Biology  
The role of alternative splicing in self-renewal, pluripotency and tissue lineage specification of human embryonic stem cells (hESCs) is largely unknown.  ...  These included an enrichment of splice isoforms that oppose cell-cycle arrest in hESCs and that promote calcium signaling and cardiac development in cardiac precursors.  ...  In loving memory of Karen Vranizan and Milo Salomonis. Author Contributions  ... 
doi:10.1371/journal.pcbi.1000553 pmid:19893621 pmcid:PMC2764345 fatcat:jgthhwg3hjgyrevs7ibybqvwea

α6-Integrin alternative splicing: distinct cytoplasmic variants in stem cell fate specification and niche interaction

Zijing Zhou, Jing Qu, Li He, Hong Peng, Ping Chen, Yong Zhou
2018 Stem Cell Research & Therapy  
Understanding of the distinct functions of α6 splicing variants in stem cell biology may inform the rational design of novel stem cell-based therapies for a range of human diseases.  ...  In this review, we summarize the current knowledge on the structural variants of the α6integrin subunit and spatiotemporal expression of α6 cytoplasmic variants in embryonic and adult stem/progenitor cells  ...  Alternative splicing impacts the function of α6 in the regulation of the stem cell propensity and niche interaction.  ... 
doi:10.1186/s13287-018-0868-3 pmid:29720266 pmcid:PMC5930856 fatcat:6kdfbv44ffahnob5b5usvcqukm

Small and long non-coding RNAs in cardiac homeostasis and regeneration

Samir Ounzain, Stefania Crippa, Thierry Pedrazzini
2013 BBA - Molecular Cell Research  
This article is part of a Special Issue entitled: Cardiomyocyte Biology: Cardiac Pathways of Differentiation, Metabolism and Contraction.  ...  Recently, the notion of promoting cardiac regeneration as a means to replace lost cardiomyocytes in the damaged heart has engendered considerable research interest.  ...  Acknowledgement We are grateful to the Swiss National Science Foundation for their support (National Research Program 63 "Stem Cells and Regenerative Medicine"; Grant no: 406340-128129).  ... 
doi:10.1016/j.bbamcr.2012.08.010 pmid:22951218 fatcat:bl6dojzynbdcrg6boklt3zlg3i

Alternative splicing: An important mechanism in stem cell biology

Kenian Chen
2015 World Journal of Stem Cells  
It has been shown that AS is prevalent in metazoan genomes, and the splicing pattern is dynamically regulated in different tissues and cell types, including embryonic stem cells.  ...  However, most of the studies focused on the expression of a core set of transcription factors and regulatory RNAs. The role of AS in stem cell differentiation was not clear.  ...  Pervasive splicing in embryonic stem cells Embryonic stem cells (ESCs) are pluripotent cells which can self-renew and has the ability to differentiate into all three germ layers [59, 60] .  ... 
doi:10.4252/wjsc.v7.i1.1 pmid:25621101 pmcid:PMC4300919 fatcat:wtxuqxgs4bghvmduzuwkos4myi

Alternative Splicing in Neurogenesis and Brain Development

Chun-Hao Su, Dhananjaya D, Woan-Yuh Tarn
2018 Frontiers in Molecular Biosciences  
Genetic manipulation and RNA sequencing have provided insights into the molecular mechanisms underlying the effects of alternative splicing in stem cell self-renewal and neuronal fate specification.  ...  Alternative splicing of many key transcription regulators or epigenetic factors reprograms the transcriptome and hence contributes to stem cell fate determination.  ...  ACKNOWLEDGMENTS The work from our laboratory that is reported in this review was supported by the Ministry of Science and Technology grant 106-2311-B-001-015.  ... 
doi:10.3389/fmolb.2018.00012 pmid:29484299 pmcid:PMC5816070 fatcat:bybazza6gve3hfcmgzddrsj24e

Alternative splicing switching in stem cell lineages

Iouri Chepelev, Xin Chen
2012 Frontiers in Biology  
In addition, recent publications have revealed another interesting molecular feature of stem cells-a distinct alternative splicing pattern.  ...  In this review, we will discuss the generality of mRNA isoform switching and its interaction with other molecular mechanisms to regulate stem cell pluripotency, as well as the reprogramming process in  ...  The work in the Chen lab has been supported by Research Grant No. 05-FY09-88 from the March of Dimes Foundation, the R00HD055052 NIH Pathway to Independence Award, R21 HD065089 and R01HD065816 from NICHD  ... 
doi:10.1007/s11515-012-1198-y pmid:23399987 pmcid:PMC3566875 fatcat:aozryy4osbegjekpzc3xhkiguu

Embryonic Stem Cells as a Model System to Elucidate Early Events in Cardiac Specification and Determination [chapter]

Gabriella Minchiotti, Cristina DAniello, Roberto Ronca, Laura Gualandi, Patrizia DellEr
2011 Embryonic Stem Cells: The Hormonal Regulation of Pluripotency and Embryogenesis  
However, the scenario of the heart inducer morphogens is still incomplete, and several hints may come from in vitro study of the embryonic stem cell (ESC) differentiation process.  ...  Embryonic Stem Cells as a Model System to Elucidate Early Events in Cardiac Specification and Determination 495 as endothelial cells.  ...  Pluripotency is a prerequisite for the subsequent coordinated differentiation of embryonic stem cells into all tissues of the body.  ... 
doi:10.5772/14304 fatcat:2rfyij63c5hhbihnws2rvkw6dq

RNA binding proteins in the regulation of heart development

Yotam Blech-Hermoni, Andrea N. Ladd
2013 International Journal of Biochemistry and Cell Biology  
A fuller understanding of the roles and substrates of these proteins could ultimately provide attractive targets for the design of therapies for congenital heart defects, cardiovascular disease, or cardiac  ...  Since the targets and functions of most RBPs -in the heart and at large -are not fully understood, this review focuses on the expression and roles of RBPs that have been implicated in specific stages of  ...  In a comparison of human embryonic stem cells before and after differentiation into either cardiac or neural progenitors, Salomonis and colleagues identified alternative splicing events common to both  ... 
doi:10.1016/j.biocel.2013.08.008 pmid:23973289 pmcid:PMC3804413 fatcat:jp7hadvr4ngothxnly6c7pyygu

Nitric oxide–cyclic GMP signaling in stem cell differentiation

Kalpana Mujoo, Joshua S. Krumenacker, Ferid Murad
2011 Free Radical Biology & Medicine  
This review will focus on some of the work pertaining to the role of NO-cGMP in differentiation of stem cells into cells of various lineages particularly into myocardial cells and stem cell based therapy  ...  in regulation of stem cell differentiation.  ...  Acknowledgments This work was supported in part by National Institutes of Health (GM076695) and grants from the Dunn foundation, the Welch foundation, Mathers foundation, NASA and the University of Texas  ... 
doi:10.1016/j.freeradbiomed.2011.09.037 pmid:22019632 pmcid:PMC3232180 fatcat:h7mi4shm4ndd5lrqombvdyh3rq

LncRNAs in Stem Cells

Shanshan Hu, Ge Shan
2016 Stem Cells International  
We discuss some of the known ncRNAs in stem cells such as embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells, adult stem cells, and cancer stem cells with a focus on long ncRNAs  ...  Stem cells occupy a special position in cell biology and Biomedicine, and emerging results show that multiple ncRNAs play essential roles in stem cells.  ...  Conflict of Interests The authors declare that there is no conflict of interests regarding the publication of this paper.  ... 
doi:10.1155/2016/2681925 pmid:26880946 pmcid:PMC4736767 fatcat:mvuq2nq75jaajpxfve6vkjjtne

Regulation of Cardiac Cell Fate by microRNAs: Implications for Heart Regeneration

Margarida Gama-Carvalho, Jorge Andrade, Luis Brás-Rosário
2014 Cells  
Here we review the role of microRNAs in the control of stem cell and cardiomyocyte dependent cardiac regeneration processes, and discuss potential applications for the treatment of cardiac injury.  ...  Both the proliferation of adult cardiac stem cells and the ability of cardiomyocytes to re-enter the cell cycle have been proposed to sustain these regenerative processes.  ...  Luis Brás-Rosário contributed to the outline of the manuscript and in manuscript revision. Conflicts of Interest The authors declare no conflict of interest.  ... 
doi:10.3390/cells3040996 pmid:25358052 pmcid:PMC4276912 fatcat:kty3ztxgvrdkxiqplgobk5ylru

The promise of enhancer-associated long noncoding RNAs in cardiac regeneration

Samir Ounzain, Thierry Pedrazzini
2015 Trends in cardiovascular medicine  
New strategies envisage the transfer of stem cells into the damaged myocardium, the mobilization of cardiac precursor cells, the promotion of cardiomyocyte proliferation in situ and direct reprogramming  ...  of non-cardiac cells into electromechanically coupled cardiomyocytes.  ...  Embryonic stem (ES) cells and induced pluripotent stem (iPS) cells are unlimited sources of precursors [2] .  ... 
doi:10.1016/j.tcm.2015.01.014 pmid:25753179 fatcat:tsosrmzpnfcmjbhj5lh7sbviee

The Emerging Role of the RBM20 and PTBP1 Ribonucleoproteins in Heart Development and Cardiovascular Diseases

Stefania Fochi, Pamela Lorenzi, Marilisa Galasso, Chiara Stefani, Elisabetta Trabetti, Donato Zipeto, Maria Grazia Romanelli
2020 Genes  
More than 90% of human genes are regulated by alternative splicing events, which participate in cell fate determination.  ...  Alternative splicing is a regulatory mechanism essential for cell differentiation and tissue organization.  ...  In human embryonic stem-cell-derived cardiomyocytes, the knockout of RBM20 affects the choice of alternative exons for both the TTN-associated genes CACNA1C and CAMK2D.  ... 
doi:10.3390/genes11040402 pmid:32276354 fatcat:xr4j2hniebfkzilyk3y2kdx524

Divergent roles of tumor suppressor gene Klf6 in ES cell differentiation

Nobuyuki Matsumoto, Xiao Zhao, Fumio Itoh, Scott L. Friedman
2008 Journal of Electrophoresis  
The aim of this study was to more clearly define the roles of Klf6 in tissue specification using tissue-specific ES cell differentiation systems.  ...  We previously reported that Klf6 -/mice are embryonic lethal because of impaired hematopoietic differentiation. In that study, we also found Klf6 -/mice have undefinable livers.  ...  We confirmed this role of Klf6 in differentiation using Klf6 -/embryonic stem (ES) cells, which displayed broad defects in differentiaton 4) .  ... 
doi:10.2198/jelectroph.52.65 fatcat:aolrjvujnvbftdn2rc2igr6o3a

Myotonic Muscular Dystrophy, RNA Toxicity, and the Brain: Trouble Making the Connection?

Mani S. Mahadevan
2011 Cell Stem Cell  
Using human embryonic stem cells (hESCs) derived from embryos with the DM1 mutation, Marteyn et al. (2011) found that after differentiation into neural precursor cells, SLITRK4 expression was decreased  ...  In this issue of Cell Stem Cell, Marteyn et al. (2011) use human embryonic stem cells to identify deficits in neuritic outgrowth in myotonic dystrophy type 1.  ...  In this issue of Cell Stem Cell, Loffredo et al. (2011) provide evidence that hematopoietic precursors do not differentiate into new cardiomyocytes but, rather, stimulate production of new cardiomyocytes  ... 
doi:10.1016/j.stem.2011.03.007 pmid:21474094 fatcat:iimn4hnlx5ctdm7svxbrdlnltm
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