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Alignment of Microtubule Imagery [article]

Feiyang Yu, Ard Oerlemans, Erwin M. Bakker
2011 arXiv   pre-print
This work discusses preliminary work aimed at simulating and visualizing the growth process of a tiny structure inside the cell---the microtubule.  ...  Here in this paper, our approach is to take pictures of microtubules at different time slots and then appropriately combine these images into a coherent video.  ...  How to align those images properly directly determines the usefulness of our video. One important feature of microtubule structure is their polarity.  ... 
arXiv:1105.6060v1 fatcat:xtqmzo5mkjbwje2j3uabpjkemm

Plant Cytoskeleton: DELLA Connects Gibberellins to Microtubules

Ram Dixit
2013 Current Biology  
The cortical microtubule cytoskeleton is a key part of the cellular machinery that defines Dispatch R479  ...  Therefore, plant growth and development relies in large part on regulation of the extent and direction of cell expansion.  ...  In addition, stable transverse alignment of cortical microtubules does not appear to be necessary for maintaining In the absence of GA, DELLA proteins hold the prefoldin complex (PFD) in the nucleus by  ... 
doi:10.1016/j.cub.2013.04.037 pmid:23743413 fatcat:5njs6nauzrgujlszpa6xwskfvu

Disruption of arabinogalactan proteins disorganizes cortical microtubules in the root of Arabidopsis thaliana

Eric Nguema-Ona, Alex Bannigan, Laurence Chevalier, Tobias I. Baskin, Azeddine Driouich
2007 The Plant Journal  
The cortical array of microtubules inside the cell and arabinogalactan proteins on the external surface of the cell are each implicated in plant morphogenesis.  ...  These findings demonstrate that cell surface arabinogalactan proteins influence the organization of cortical microtubules.  ...  Acknowledgements We thank Dr Ludovic Galas (Plateforme de Recherche en Imagerie Cellulaire de Haute-Normandie, RIO, University of Rouen), Dr Ursula Meindl-Lü tsz and Magdalena Eder (University of Salzburg  ... 
doi:10.1111/j.1365-313x.2007.03224.x pmid:17672840 fatcat:2aoijorxxbdgvdhl5dppf6ouuu

Mirror-imaged doublets of Tetmemena pustulata: Implications for the development of left–right asymmetry

Aaron J. Bell, Peter Satir, Gary W. Grimes
2008 Developmental Biology  
True mirror imagery of the membranelles would require new enantiomorphic forms of the individual ciliary components, particularly the basal bodies, which is never observed.  ...  The overall symmetry of the OA thus appears to be separable from the more localized assembly of individual basal bodies.  ...  Finally, we thank the imaging facility at AECOM for assistance with some of the image preparation.  ... 
doi:10.1016/j.ydbio.2007.11.020 pmid:18164285 fatcat:ow4c46ubmndnzoa2cpwdk5ot54

Spindle and Chromosomal Alterations in Metaphase II Oocytes

Rakesh K. Sharma, Ali Azeem, Ashok Agarwal
2013 Reproductive Sciences  
Advances in spindle imagery have made it possible to visualize the effects of environmental stresses on spindle structure.  ...  A comprehensive understanding of the spindle behavior is paramount for the effective manipulation of oocytes in an assisted reproductive setting.  ...  Declaration of Conflicting Interests The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.  ... 
doi:10.1177/1933719113483018 pmid:23536572 fatcat:i24viqmhwvdojbjke57oaognpm

Resolving Trypanosoma brucei Flagellar Structure by Cryo-Electron Tomography

Jennifer Dai, Z.B. Xu, D.Q. Chen, J.Y. Liu
2019 E3S Web of Conferences  
The present study demonstrates the detailed structure and defines the length of the axoneme and three domains of the paraflagellar rod (PFR) using cryo-electron tomography of Trypanosoma brucei flagella  ...  The key feature of these parasites is that they have a flagellum consists of a typical 9 + 2 axoneme and a lattice-like paraflagellar rod (PFR).  ...  To brief introduce, the series were analyzed by performing the following steps: removing X-rays section, creating a coarse-aligned stack, using patch tracking to create a fiducial model, aligning the serial  ... 
doi:10.1051/e3sconf/201913101012 fatcat:ykk5qshkzbbqxguwiserhxmite

Biophysical forces rewire cell metabolism to guide microtubule-dependent cell mechanics [article]

Stephanie TORRINO, Stephane Audebert, Ilyes BELHADJ, Caroline LACOUX, Sophie ABELANET, Frederic BRAU, Sabrina PISANO, Stephen Y CHAN, Bernard MARI, William M Oldham, Thomas BERTERO
2020 biorxiv/medrxiv   pre-print
Cells withstand external forces by adjusting the stiffness of its cytoskeleton. Microtubules (MTs) act as compression-bearing elements in response to mechanical cues.  ...  loads of their environment.  ...  (e) Representative alignment of microtubule in cells in presence of glutamate for 24h.  ... 
doi:10.1101/2020.03.10.985036 fatcat:xp4bxjfj2veyzegg6ndxcjhuq4

IFT88 controls NuMA enrichment at k-fibers minus-ends to facilitate their re-anchoring into mitotic spindles

Nicolas Taulet, Audrey Douanier, Benjamin Vitre, Christelle Anguille, Justine Maurin, Yann Dromard, Virginie Georget, Benedicte Delaval
2019 Scientific Reports  
This spatial regulation is required to allow proper chromosomes alignment through the organization of kinetochore fibers (k-fibers).  ...  To build and maintain mitotic spindle architecture, molecular motors exert spatially regulated forces on microtubules (MT) minus-ends.  ...  We thank the engineers on the MRI facility, Simon Descamps and Audrey Guesdon for their help with cell culture and buffers and the members of the team for discussions on the project.  ... 
doi:10.1038/s41598-019-46605-x pmid:31312011 pmcid:PMC6635507 fatcat:tfh52rhnwzbvfpkaoqmdsimih4

Plant cell shape: modulators and measurements

Alexander Ivakov, Staffan Persson
2013 Frontiers in Plant Science  
These processes include cell wall synthesis, activity of the actin and microtubule cytoskeletons, in particular their regulation by microtubule associated proteins, actin-related proteins, GTP'ases and  ...  Finally, we review recent examples of the use of novel imaging techniques and how they have contributed to our understanding of cell shape formation.  ...  Cellulose microfibrils have long been observed to co-align with microtubules in the cell cortex (Green, 1962) .  ... 
doi:10.3389/fpls.2013.00439 pmid:24312104 pmcid:PMC3832843 fatcat:difq2z45t5hhnjselhawchouhy

The plasma membrane as a mechanotransducer in plants

Leia Colin, Olivier Hamant
2021 Comptes rendus. Biologies  
In plant cells, cortical tension is of osmotic origin.  ...  The plasma membrane is a physical boundary made of amphiphilic lipid molecules, proteins and carbohydrates extensions.  ...  Using this system, cortical microtubules were shown to align with minimal curvature when protoplasts are depressurized (arguably following a minimum of energy derived from the microtubule persistence length  ... 
doi:10.5802/crbiol.66 pmid:35787608 fatcat:7gjkouzlabgz3lqgxrj7hjcnri

Rough Deal and Zw10 are required for the metaphase checkpoint in Drosophila

Renata Basto, Rui Gomes, Roger E. Karess
2000 Nature Cell Biology  
Montel for technical assistance and Lautredou Nicole from the Center of Research in Imagerie (CRIC) in confocal assistance. S. Kearsey and M.  ...  We thank all the members of the Maro laboratory for their support during the course of this work. We thank P.  ...  (C) Amino acid sequence alignments of the DOC1R protein from different vertebrate species.  ... 
doi:10.1038/35046592 pmid:11146659 fatcat:gzdhkjsktzh6nbcta4eug2kidm

Aligned electrospun fibers for neural patterning

Erfan Soliman, Fabio Bianchi, James N. Sleigh, Julian H. George, M. Zameel Cader, Zhanfeng Cui, Hua Ye
2018 Biotechnology Letters  
Results A novel cell culture system was designed by engineering a method for the directional growth of neurons.  ...  This uses NG108-15 neuroblastoma x glioma hybrid cells cultured on suspended and aligned electrospun fibers.  ...  The support of the Laboratory for Insitu Microscopy and Analysis (LIMA) and that of Christ Church, Oxford is also gratefully acknowledged.  ... 
doi:10.1007/s10529-017-2494-z pmid:29313254 pmcid:PMC5856867 fatcat:zyhtak4qvrfm3hgjmaq674uimm

Super-resolution reconstruction of cytoskeleton image based on A-net deep learning network [article]

Qian Chen, Haoxin Bai, Bingchen Che, Tianyun Zhao, Ce Zhang, Kaige Wang, Jintao Bai, Wei Zhao
2021 arXiv   pre-print
reconstruction of biomolecules in vivo (i.e. ~24 nm thickness of microtubule fiber).  ...  Even though super-resolution microscopy methods have enabled visualization of subcellular structures below the optical resolution limit, the spatial resolution is still far from enough for the structural  ...  and ~ 24 nm thickness of microtubule fiber [4].  ... 
arXiv:2112.09574v1 fatcat:2ddrs26odbhkxeybnwcjqpemly

Epidermal development requires ninein for spindle orientation and cortical microtubule organization

Nicolas Lecland, Chiung-Yueh Hsu, Cécile Chemin, Andreas Merdes, Christiane Bierkamp
2019 Life Science Alliance  
Second, ninein is required for the cortical organization of microtubules in differentiating keratinocytes, and for the cortical re-localization of microtubule-organizing proteins, and may thus affect any  ...  In mammalian skin, ninein localizes to the centrosomes of progenitor cells and relocates to the cell cortex upon differentiation of keratinocytes, where cortical arrays of microtubules are formed.  ...  We thank A Davy, C Audouard, M Djabali, and P Mercier for their help with the generation of mutant mice, and T Jungas and T Mangeat for further technical help.  ... 
doi:10.26508/lsa.201900373 pmid:30923192 pmcid:PMC6441496 fatcat:es2inkctdff6bnftsoupi45vki

IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis

Benjamin Vitre, Nicolas Taulet, Audrey Guesdon, Audrey Douanier, Aurelie Dosdane, Melanie Cisneros, Justine Maurin, Sabrina Hettinger, Christelle Anguille, Michael Taschner, Esben Lorentzen, Benedicte Delaval
2020 EMBO Reports  
Here, using siRNA knock-down of various IFT proteins or AID-inducible degradation of endogenous IFT88 in combination with small-molecule inhibition of HSET, we show that IFT proteins together with HSET  ...  Finally, we demonstrate the requirement of IFT88 for efficient centrosome clustering in a variety of cancer cell lines naturally harboring supernumerary centrosomes and its importance for cancer cell proliferation  ...  Acknowledgements The experiments were performed on the imaging platform Montpellier Ressources Imagerie (MRI), Montpellier, member of the national infrastructure France-BioImaging supported by the French  ... 
doi:10.15252/embr.201949234 pmid:32270908 fatcat:rjgz4js7b5hjdjaairspmni5i4
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