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Strategies in Glioma-Surgery [chapter]

Sven R., Alf Giese
2011 Diagnostic Techniques and Surgical Management of Brain Tumors  
Although these operations have shown some limited success they were unable to finally cure glioma (Giese et al., 2003) .  ...  Furthermore most recurrent glioma arise at its primary resection site or in a distance of no more than 2-3cm there off (Giese et al., 2003) .  ...  Kantelhardt and Alf Giese (2011) . Strategies in Glioma-Surgery, Diagnostic Techniques and Surgical Management of Brain Tumors, Dr.  ... 
doi:10.5772/22085 fatcat:vulrpdpckjaizpxzjp42mge3o4

Allogeneic Astrocytoma In Immune Competent Dogs

Michael E. Berens, Alf Giese, Joan R. Shapiro, Stephen W. Coons
1999 Neoplasia: An International Journal for Oncology Research  
We have induced in canines long-term immune tolerance to an allogeneic cell line derived from a spontaneous canine astrocytoma. Allogeneic astrocytoma cells were implanted endoscopically into the subcutaneous space of fetal dogs before the onset of immune ( ) competency -----40th gestational day . At adulthood, dogs rendered tolerant successfully serve as recipients of intracranial transplants of their growing allogeneic, subcutaneous tumor. Transplanted dogs subsequently develop a solid brain
more » ... umor with histological features similar to the original astrocytoma. This model may allow rapid development and evaluation of new therapies for brain tumors, as well as afford tumor biology studies that are untenable in smaller, immune incompetent, or inbred animals harboring less representative tumors.
doi:10.1038/sj.neo.7900020 pmid:10933043 pmcid:PMC1508127 fatcat:biq2t37g2bbhndnxlhvsgvqbwa

"...those left behind." Biology and Oncology of Invasive Glioma Cells

Michael E Berens, Alf Giese
1999 Neoplasia: An International Journal for Oncology Research  
doi:10.1038/sj.neo.7900034 pmid:10935475 pmcid:PMC1508082 fatcat:bfdvht6rbrcn3dwxacuoprcin4

3D-Planung von Trajektorie und Objekt bei neurochirurgischen Wirbelsäuleneingriffen

Michael Kosterhon, Angelika Gutenberg, Eike S. Schwandt, Jens Conrad, Sven R. Kantelhardt, A. Nimer Amr, Joachim Gawehn, Alf Giese
2012 CURAC Jahrestagung  
Wir stellen eine neue Möglichkeit zur dreidimensionalen, präoperativen Planung vor, die nicht nur die Planung von minimalinvasiven Zugangswegen zur Wirbelsäule, sondern darüber hinaus auch eine 3D Modellierung von Instrumenten und die präoperative virtuelle Platzierung von realen Implantaten erlauben soll. Wir testeten diese Vorgehensweise erstmals im Rahmen des Débridement bei einer Patientin mit Spondylodiscitis.
dblp:conf/curac/KosterhonGSCKAG12 fatcat:5wxllcjyqffztfjita6xpgamfi

Thromboxane synthase regulates the migratory phenotype of human glioma cells

Alf Giese, Christian Hagel, Ella L. Kim, Svenja Zapf, Jasmin Djawaheri, Michael E. Berens, Manfred Westphal
1999 Neuro-Oncology  
et al., 1994 Giese et al., , 1995 Giese et al., , 1996a .  ...  ., 1991; Giese et al., 1996c; Paulus and Tonn, 1994) .  ... 
doi:10.1215/s1522851798000143 pmid:11550298 pmcid:PMC1919460 fatcat:hphdopg3gbbihhgkwcqfs44jce

No role of IFITM3 in brain tumor formation in vivo

Nevenka Dudvarski Stankovic, Nicola Hoppmann, Marcin Teodorczyk, Ella L. Kim, Matthias Bros, Alf Giese, Frauke Zipp, Mirko H.H. Schmidt
2016 OncoTarget  
Glioblastoma multiforme (GBM) is one of the most lethal solid tumors in adults. Despite aggressive treatment approaches for patients, GBM recurrence is inevitable, in part due to the existence of stem-like brain tumor-propagating cells (BTPCs), which produce factors rendering them resistant to radio-and chemotherapy. Comparative transcriptome analysis of irradiated, patient-derived BTPCs revealed a significant upregulation of the interferon-inducible transmembrane protein 3 (IFITM3), suggesting
more » ... the protein as a factor mediating radio resistance. Previously, IFITM3 has been described to affect glioma cells; therefore, the role of IFITM3 in the formation and progression of brain tumors has been investigated in vivo. Intracranial implantation studies using radio-selected BTPCs alongside non-irradiated parental BTPCs in immunodeficient mice displayed no influence of irradiation on animal survival. Furthermore, gain and loss of function studies using BTPCs ectopically expressing IFITM3 or having IFITM3 down-modulated by a shRNA approach, did affect neither tumor growth nor animal survival. Additionally, a syngeneic model based on the mouse glioma cell line GL261 was applied in order to consider the possibility that IFITM3 relies on an intact immune system to unfold its tumorigenic potential. GL261 cells ectopically expressing IFITM3 were implanted into the striatum of immunocompetent mice without influencing the survival of glioma-bearing animals. Lastly, the vasculature and the extent of microglia/macrophage invasion into the tumor were studied in BTPC and GL261 tumors but neither parameter was altered by IFITM3. This report presents for the first time that IFITM3 is upregulated in patient-derived BTPCs upon irradiation but does not affect brain tumor formation or progression in vivo. Meier survival curves ( Figure 2G ; median survival 60.5 vs 62 d in control animals; n = 8; P > 0.05).
doi:10.18632/oncotarget.13199 pmid:27835870 pmcid:PMC5349921 fatcat:e6hca5jupjc4tavalbcgclbqoe

Characterization of the amplicon on chromosomal segment 4q12 in glioblastoma multiforme

Nikola Holtkamp, Nicolas Ziegenhagen, Elke Malzer, Christian Hartmann, Alf Giese, Andreas von Deimling
2007 Neuro-Oncology  
A subset of glioblastomas (GBMs) carry gene amplifications on chromosomal segment 4q12. To characterize this amplicon in detail, we analyzed a set of 100 samples consisting of 65 GBMs, 10 WHO grade III astrocytomas, 12 oligodendrogliomas, and 13 glioma cell cultures. We applied multiplex ligation-dependent probe amplification to determine the gene dosage of PDG-FR A, KIT, and KDR and the flanking genes USP46, RASL11B, LNX1, CHIC2, SEC3L1, and IGFBP7. The amplicon was highly variable in size and
more » ... copy number and extended over a region of up to 5 Mb. Amplifications on 4q12 were observed in 15% of GBMs and 23% of GBM cell cultures but not in 22 other gliomas. We analyzed transcription and translation of some genes within this amplicon. Gene amplification generally correlated with high transcript levels but did not necessarily result in increased protein levels. However, we detected frequent expression of proteins encoded by PDGFRA, KIT, and KDR in GBMs and GBM cell cultures independent of the amplification status. Future treatment of GBM patients may include drugs targeting multiple kinases that are encoded by genes on chromosomal segment 4q12. Neuro-Oncology 9, 291-297, 2007 (Posted to Neuro-Oncology [serial online], Doc. D06-00147, May 15, 2007
doi:10.1215/15228517-2007-009 pmid:17504929 pmcid:PMC1907422 fatcat:jqi4nkgcsjgyplptkfcscbtoou

Technical Aids in the Diagnosis of Brain Death

Stefan Welschehold, Stephan Boor, Katharina Reuland, Frank Thömke, Thomas Kerz, André Reuland, Christian Beyer, Martin Gartenschläger, Wolfgang Wagner, Alf Giese, Wibke Müller-Forell
2012 Deutsches Ärzteblatt International  
Conflict of interest statement Thömke, Boor, Wagner, Reuland K, Reuland A, Welschehold, Müller-Forell, Giese: These authors declare that no conflict of interest exists. Dr.  ... 
doi:10.3238/arztebl.2012.0624 pmid:23093994 pmcid:PMC3475291 fatcat:67eltjr2xrevddmw64wviktxcq

Tamoxifen-resistant glioma-cell sub-populations are characterized by increased migration and proliferation

Maximilian J. A. Puchner, Alf Giese
2000 International Journal of Cancer  
Tumor-cell-adhesion assays Glioma cells were tested for substrate adhesion as described (Giese et al., 1999) .  ...  We have demonstrated that highly migratory glioma cells over-express thromboxane synthase (Giese et al., 1999) .  ... 
doi:10.1002/(sici)1097-0215(20000515)86:4<468::aid-ijc4>;2-r pmid:10797257 fatcat:bjyzmyplhva6bdx6qlziyd2zfe

Bedeutung der 3D-OP-Planung und 3D-Navigation für die minimalinvasive Neurochirurgie

Eike S. Schwandt, Sven R. Kantelhardt, Ali Ayyad, Michael Kosterhon, Axel T. Stadie, Alf Giese
2012 CURAC Jahrestagung  
Seit 2004 wird zur dreidimensionalen OP-Planung in der Mainzer Neurochirurgie das Dextroscope-System genutzt, um neurochirurgische Eingriffe durch Optimierung der Zugangswege und Vorgehensweisen sicherer und schonender durchführen zu können. Hierzu werden zweidimensionale Schichtbildaufnahmen fusioniert. Weltweit werden mittlerweile in den Operationssälen nahezu jeder größeren neurochirurgischen Abteilung Navigationsgeräte eingesetzt, die dem Chirurgen intraoperativ die Orientierung
more » ... Auch diese Geräte verrechnen zweidimensionale Schichtbildaufnahmen und ermöglichen in diesen die Darstellung der exakten Position von OP-Instrumenten. Da es leider bislang nicht möglich war, die mit dem Dextroscope-System erstellten 3D-Planungsdaten zur intraoperativen Navigation zu nutzen, unternahmen wir nun erste Schritte, um mit präoperativ dreidimensional erstellten Planungsdaten intraoperativ dreidimensional navigieren zu können.
dblp:conf/curac/SchwandtKAKSG12 fatcat:6p6wb35xm5fyhkwv76op6d7g6y

Navigation and robot-aided surgery in the spine: historical review and state of the art

Sven Kantelhardt, Nimer Amr, Alf Giese
2014 Robotic Surgery Research and Reviews  
Spinal anatomy is complex and in close vicinity of vulnerable anatomical structures, such as nerve roots, spinal cord, and blood vessels, requiring high precision in surgical procedures, while the surgical exposure is limited and even more limited in percutaneous procedures. A high risk of misplaced implants and/or high intraoperative radiation exposure are the consequences. Spinal navigation techniques have been introduced to alleviate these problems. The majority of reports on navigation
more » ... ent its role in increasing implant-placement accuracy and reducing intraoperative radiation. The technology is increasingly finding acceptance amongst spinal surgeons. This however does not yet present the end of the road. Robotic applications could be regarded as a coherent continuation of spinal navigation. We reviewed the literature pertaining to spinal navigation, as well as novel and upcoming robotic applications for spinal surgery. For this purpose, a Medline search using the terms "robot" and "spine" and extensive cross-reference analysis (using Medline, Ovid, and Web of Science data banks) were performed. The goal was to provide an overview of present achievements and novel developments in this fast-growing research area. While a number of robotic techniques are under investigation, mainly to aid navigated screw placement, the SpineAssist/Renaissance system has cleared US Food and Drug Administration approval and is in relatively wide clinical use. Another robotic system, the da Vinci, is currently applied in an increasing number of cases under research protocols.
doi:10.2147/rsrr.s54390 fatcat:lsj5utdn7vhvpntgumygnaykmm

Inhibition of Thromboxane Synthase Activity Improves Glioblastoma Response to Alkylation Chemotherapy

Nils Ole Schmidt, Mateo Ziu, Theresa Cargioli, Manfred Westphal, Alf Giese, Peter M. Black, Rona S. Carroll
2010 Translational Oncology  
Thromboxane synthase (TXSA), an enzyme of the arachidonic acid metabolism, is upregulated in human glial tumors and is involved in glioma progression. Here, we analyzed the in vitro and in vivo effects of pharmacological inhibition of TXSA activity on human glioblastoma cells. Furegrelate, a specific inhibitor of TXSA, significantly inhibited tumor growth in an orthotopic glioblastoma model by inducing proapoptotic, antiproliferative, and antiangiogenic effects. Inhibition of TXSA induced a
more » ... poptotic disposition of glioma cells and increased the sensitivity to the chemotherapeutic agent 1,3-bis(2-chloroethyl)-1-nitrosourea, significantly prolonging the survival time of intracerebral glioma-bearing mice. Our data demonstrate that the targeted inhibition of TXSA activity improves the efficiency of conventional alkylation chemotherapy in vivo. Our study supports the role of TXSA activity for the progression of malignant glioma and the potential utility of its therapeutic modulation for glioma treatment.
doi:10.1593/tlo.09238 pmid:20165694 pmcid:PMC2822453 fatcat:dom3ebrbdfcxhll7cbli5suzaa

Gamma Irradiation Triggers Immune Escape in Glioma-Propagating Cells

Nicola Hoppmann, Nora Heinig, Ute Distler, Ella Kim, Volker Lennerz, Yvonne Krauß, Ulrike Schumann, Alf Giese, Stefan Tenzer, Lynn Bitar, Mirko H. H. Schmidt
2022 Cancers  
Glioblastoma multiforme is the most common and devastating form of brain tumor for which only palliative radio- and chemotherapy exists. Although some clinical studies on vaccination approaches have shown promising efficacy due to their potential to generate long-term immune surveillance against cancer cells, the evasion mechanisms preventing therapy response are largely uncharacterized. Here, we studied the response of glioblastoma-propagating cells (GPCs) to clinically relevant doses of γ
more » ... ation. GPCs were treated with 2.5 Gy of γ radiation in seven consecutive cellular passages to select for GPCs with increased colony-forming properties and intrinsic or radiation-induced resistance (rsGPCs). Quantitative proteomic analysis of the cellular signaling platforms of the detergent-resistant membranes (lipid rafts) in GPCs vs. rsGPCs revealed a downregulation of the MHC class I antigen-processing and -presentation machinery. Importantly, the radio-selected GPCs showed reduced susceptibility towards cytotoxic CD8+ T-cell-mediated killing. While previous studies suggested that high-dose irradiation results in enhanced antigen presentation, we demonstrated that clinically relevant sub-lethal fractionated irradiation results in reduced expression of components of the MHC class I antigen-processing and -presentation pathway leading to immune escape.
doi:10.3390/cancers14112728 pmid:35681710 pmcid:PMC9179833 fatcat:aepbqcid3vfiraxch7u44p6gfq

Gap junction intercellular communication in gliomas is inversely related to cell motility

Wendy S McDonough, Angie Johansson, Hylton Joffee, Alf Giese, Michael E Berens
1999 International Journal of Developmental Neuroscience  
AbstractÐGliomas are lethal because of local invasion into brain parenchyma. Glioma cells were isolated from dierent regions (white matter, gray matter and tumor core) of a glioma-bearing dog brain. Individual clonal cell lines were established from each area, and characterized for growth, migration and gap junctions. The regional clonal cell lines diered in rates and preferred substrate for migration. Cell lines generated from invaded white matter showed stimulated migration on collagen and
more » ... iable migration on merosin, whereas migration of cell lines derived from invaded gray matter showed the reciprocal responses: stimulation on merosin and inhibition on collagen. Gap junctional communication showed signi®cant degrees of variation between the dierent clones. A direct inverse relationship between the number of cells demonstrating gap junctional communication and migration rate of cells away from multicellular spheroids was evident. Glioma cells which have a reduced capacity to connect to each other have an accelerated migration rate onto autologous, glioma-derived matrix. These results suggest that invasive glioma cells suppress autologous cell-to-cell cohesion, partly evident as reduced formation of gap junctions. In addition, glioma cells were stimulated to migrate in a dose-dependant manner in response to epidermal growth factor (EGF) coincident with the reduction of Cx43 levels and increased serine phosphorylation. We speculate that in order for glioma cells to invade locally into brain parenchyma they must ®rst detach from neighboring cells ("let go...let's go" paradigm of invasion). #
doi:10.1016/s0736-5748(99)00024-6 pmid:10571421 fatcat:6oskzqzqobhnbfumsvhnqtm5my
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