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A Method to Characterize Calcium Activity in Stimulated Cultures of Cardiac Myocytes

Raul Benitez, Alex Vallmitjana, Leif Hove-Madsen
2013 Biophysical Journal  
Vallmitjana 1 , Leif Hove-Madsen 2 .  ...  density Wednesday, February 6, 2013 607a Membrane Receptors & Signal Transduction II Board B280 A Method to Characterize Calcium Activity in Stimulated Cultures of Cardiac Myocytes Raul Benitez 1 , Alex  ... 
doi:10.1016/j.bpj.2012.11.3365 fatcat:gnuroghpobb3zbynpig5kexaw4

Simultaneous Detection and Colocalization of Calcium Sparks and Ryanodine Receptor Clusters in Cardiac Myocytes

Alex Vallmitjana, Florian Hiess, S.R. Wayne Chen, Leif Hove-Madsen, Raul Benitez
2015 Biophysical Journal  
Board B276 Simultaneous Detection and Colocalization of Calcium Sparks and Ryanodine Receptor Clusters in Cardiac Myocytes Alex Vallmitjana 1 , Florian Hiess 2 , S.R.  ... 
doi:10.1016/j.bpj.2014.11.1447 fatcat:evyz75c6uvcjlnnru554tfc2ne

Superresolution Imaging of RYR2 Clusters in GFP RYR2 Knock in Mouse Cardiomyocytes

Florian Hiess, Ruiwu Wang, Alex Vallmitjana, David R.L. Scriven, Leif Hove-Madsen, Raul Benítez, Edwin D.W. Moore, S.R. Wayne Chen
2014 Biophysical Journal  
Board B738 Superresolution Imaging of RYR2 Clusters in GFP RYR2 Knock in Mouse Cardiomyocytes Florian Hiess 1 , Ruiwu Wang 1 , Alex Vallmitjana 2 , David R.L.  ... 
doi:10.1016/j.bpj.2013.11.2245 fatcat:pouo5flcfjbi7laulyhleelf7m

Mitochondria and calcium defects correlate with axonal dysfunction in GDAP1-related Charcot-Marie-Tooth mouse model

Azahara Civera-Tregón, Laura Domínguez, Paula Martínez-Valero, Clàudia Serrano, Alex Vallmitjana, Raúl Benítez, Janet Hoenicka, Jorgina Satrústegui, Francesc Palau
2021 Neurobiology of Disease  
The pipeline is described in detail in Vallmitjana et al. (Vallmitjana et al., 2017) , but here we give a brief description of the video tracking algorithm.  ... 
doi:10.1016/j.nbd.2021.105300 pmid:33582224 fatcat:gdhknlg65rdgneyvqcgiknzphq

Adenosine A2A Receptor Activation Decreases Beat-To-Beat Stability of Intracellular Calcium Transients and their Propagation in Atrial Myocytes

Barriga Montserrat, Cristina E. Molina, Anna Llach, Nuria Cabello, Alex Vallmitjana, Raul Benitez, Josep Padro, Juan Cinca, Leif Hove-Madsen
2012 Biophysical Journal  
Molina 2 , Anna Llach 2 , Nuria Cabello 1 , Alex Vallmitjana 3 , Raul Benitez 3 , Josep Padro 2 , Juan Cinca 2 , Leif Hove-Madsen 1 . 1 CSIC, Barcelona, Spain, 2 Hospital de Sant Pau, Barcelona, Spain,  ... 
doi:10.1016/j.bpj.2011.11.3017 fatcat:stccmljocfanjm6fplaejpnqtm

Cardiac electrical defects in progeroid mice and Hutchinson–Gilford progeria syndrome patients with nuclear lamina alterations

José Rivera-Torres, Conrado J. Calvo, Anna Llach, Gabriela Guzmán-Martínez, Ricardo Caballero, Cristina González-Gómez, Luis J. Jiménez-Borreguero, Juan A. Guadix, Fernando G. Osorio, Carlos López-Otín, Adela Herraiz-Martínez, Nuria Cabello (+10 others)
2016 Proceedings of the National Academy of Sciences of the United States of America  
Hutchinson-Gilford progeria syndrome (HGPS) is a rare genetic disease caused by defective prelamin A processing, leading to nuclear lamina alterations, severe cardiovascular pathology, and premature death. Prelamin A alterations also occur in physiological aging. It remains unknown how defective prelamin A processing affects the cardiac rhythm. We show age-dependent cardiac repolarization abnormalities in HGPS patients that are also present in the Zmpste24 −/− mouse model of HGPS. Challenge of
more » ... mpste24 −/− mice with the β-adrenergic agonist isoproterenol did not trigger ventricular arrhythmia but caused bradycardia-related premature ventricular complexes and slow-rate polymorphic ventricular rhythms during recovery. Patch-clamping in Zmpste24 −/− cardiomyocytes revealed prolonged calcium-transient duration and reduced sarcoplasmic reticulum calcium loading and release, consistent with the absence of isoproterenol-induced ventricular arrhythmia. Zmpste24 −/− progeroid mice also developed severe fibrosis-unrelated bradycardia and PQ interval and QRS complex prolongation. These conduction defects were accompanied by overt mislocalization of the gap junction protein connexin43 (Cx43). Remarkably, Cx43 mislocalization was also evident in autopsied left ventricle tissue from HGPS patients, suggesting intercellular connectivity alterations at late stages of the disease. The similarities between HGPS patients and progeroid mice reported here strongly suggest that defective cardiac repolarization and cardiomyocyte connectivity are important abnormalities in the HGPS pathogenesis that increase the risk of arrhythmia and premature death. Hutchinson-Gilford progeria syndrome | progerin | prelamin A | connexin43 | calcium handling T he LMNA gene encodes A-type lamins (lamin A and lamin C), key components of the mammalian nuclear envelope with important structural and regulatory functions that affect signaling, transcription, and chromatin organization among other processes (1). Mature lamin A is produced from the precursor prelamin A through a series of posttranslational modifications, consisting of sequential farnesylation at the cysteine in the Cysteine-Serine-Isoleucine-Methionine motif, cleavage of the Serine-Isoleucine-Methionine residues, carboxymethylation of the newly accessible cysteine, and a final proteolytic cleavage by the zinc metallopeptidase STE24 (ZMPSTE24, also called FACE-1) (2). Mutations in the human LMNA gene or defective processing of prelamin A cause a group of diseases termed laminopathies, including the premature aging disorder Hutchinson-Gilford progeria syndrome (HGPS), a very rare genetic disorder with an estimated prevalence of 1 in 21 million people (www.progeriaresearch.org). Significance Defective prelamin A processing causes cardiovascular alterations and premature death in Hutchinson-Gilford progeria syndrome (HGPS) patients and also occurs during physiological aging. We found overt repolarization abnormalities in HGPS patients at advanced disease stages. Similar alterations were present in progeroid Zmpste24 −/− mice, which had cardiomyocytes that exhibited prolonged calcium transient duration and reduced sarcoplasmic reticulum calcium loading capacity and release, consistent with absence of isoproterenolinduced ventricular arrhythmias. Zmpste24 −/− mice developed age-dependent bradycardia and PQ interval/QRS complex prolongation, likely contributing to premature death. These defects correlated with mislocalization of connexin43, which was also noted in heart tissue from HGPS patients. These results reveal molecular alterations that might cause cardiac rhythm alterations and premature death in HGPS.
doi:10.1073/pnas.1603754113 pmid:27799555 pmcid:PMC5135377 fatcat:tbx3x3l4xjhvjdcdyovj7sa4gq

Supplementary document for Phasor space-based image segmentation: machine learning clustering techniques - 5111012.pdf

Alexander Vallmitjana, Belen Torrado, Enrico Gratton
2021 figshare.com  
LIFETIME AND SPECTRAL IMAGE SEGMENTATION BASED ON PHASOR SPACE CLUSTERING ALEX VALLMITJANA, BELÉN TORRADO AND ENRICO GRATTON Laboratory for Fluorescence Dynamics, Biomedical Engineering, University of  ... 
doi:10.6084/m9.figshare.14473578.v2 fatcat:nwk2uddzangtlommd7jxw7ztuy

Supplementary document for Phasor space-based image segmentation: machine learning clustering techniques - 5111012.pdf

Alexander Vallmitjana, Belen Torrado, Enrico Gratton
2021 figshare.com  
LIFETIME AND SPECTRAL IMAGE SEGMENTATION BASED ON PHASOR SPACE CLUSTERING ALEX VALLMITJANA, BELÉN TORRADO AND ENRICO GRATTON Laboratory for Fluorescence Dynamics, Biomedical Engineering, University of  ... 
doi:10.6084/m9.figshare.14473578.v1 fatcat:yl4b4hkepvcrxc3qzruk2ics7m

Structural and Functional Defects of T-Tubular System and Their Implications in Calcium Release and Contraction in a Mouse Model of Hypertrophic Cardiomyopathy

Claudia Crocini, Cecilia Ferrantini, Raffaele Coppini, Marina Scardigli, Erica Lazzeri, Claudio Chicchi, Ping Yan, Leslie M. Loew, Jill Tardif, Chiara Tesi, Francesco Vanzi, Elisabetta Cerbai (+2 others)
2015 Biophysical Journal  
Board B276 Simultaneous Detection and Colocalization of Calcium Sparks and Ryanodine Receptor Clusters in Cardiac Myocytes Alex Vallmitjana 1 , Florian Hiess 2 , S.R.  ... 
doi:10.1016/j.bpj.2014.11.1446 fatcat:iexrk3qiojbkpdwzwicna3rj44

Expression and Function of Inositol 1,4,5-Trisphosphate Receptors in the Heart

Martha Iveth Garcia, Darren Boehning
2015 Biophysical Journal  
Board B276 Simultaneous Detection and Colocalization of Calcium Sparks and Ryanodine Receptor Clusters in Cardiac Myocytes Alex Vallmitjana 1 , Florian Hiess 2 , S.R.  ... 
doi:10.1016/j.bpj.2014.11.1449 fatcat:sxsj3qjzcnbc7hc2agpkahhjiu

Reduced Ca Flux via Ryanodine Receptor Cluster Size or Unitary Current can both Promote Long-Lasting Ca Sparks

Daisuke Sato, Thomas R. Shannon, Donald M. Bers
2015 Biophysical Journal  
Board B276 Simultaneous Detection and Colocalization of Calcium Sparks and Ryanodine Receptor Clusters in Cardiac Myocytes Alex Vallmitjana 1 , Florian Hiess 2 , S.R.  ... 
doi:10.1016/j.bpj.2014.11.1448 fatcat:eybkwligubajrpq35rhurgvizm

Local Control Model Illustrates How Action Potential Morphology Affects Ca2+ Release

Laura D. Gauthier, Joseph L. Greenstein, Raimond L. Winslow
2012 Biophysical Journal  
Molina 2 , Anna Llach 2 , Nuria Cabello 1 , Alex Vallmitjana 3 , Raul Benitez 3 , Josep Padro 2 , Juan Cinca 2 , Leif Hove-Madsen 1 . 1 CSIC, Barcelona, Spain, 2 Hospital de Sant Pau, Barcelona, Spain,  ... 
doi:10.1016/j.bpj.2011.11.3016 fatcat:xxeqv4bpezgsfgtpmhc4oqaqcq

TRPC1 and TRPC4 Channels Contribute to Basal Cardiac Calcium Signalling via a Constitutively Active Background Calcium Entry

Peter Lipp, Qinghai Tian, Martin Oberhofer, Karin Hammer, Juan E. Camacho-Londono, Petra Weissgerber, Marc Freichel, Lars Kaestner
2012 Biophysical Journal  
Molina 2 , Anna Llach 2 , Nuria Cabello 1 , Alex Vallmitjana 3 , Raul Benitez 3 , Josep Padro 2 , Juan Cinca 2 , Leif Hove-Madsen 1 . 1 CSIC, Barcelona, Spain, 2 Hospital de Sant Pau, Barcelona, Spain,  ... 
doi:10.1016/j.bpj.2011.11.3014 fatcat:y7onwrh5b5hrjev5uwv4uw25wu

Relationship Between Ca2+ Alternans and T-Wave Alternans: Role of Calsequestrin and Sorcin

Azade D. Petrosky, Bernardo Zepeda, Alexander B. Petrosky, Dmytro Kornyeyev, Paola Contreras, Hector H. Valdivia, Ariel L. Escobar
2012 Biophysical Journal  
Molina 2 , Anna Llach 2 , Nuria Cabello 1 , Alex Vallmitjana 3 , Raul Benitez 3 , Josep Padro 2 , Juan Cinca 2 , Leif Hove-Madsen 1 . 1 CSIC, Barcelona, Spain, 2 Hospital de Sant Pau, Barcelona, Spain,  ... 
doi:10.1016/j.bpj.2011.11.3015 fatcat:uvdawi5rubcuvem2cwkw4y3lte

Store-Operated Ca2+ Entry is a Tonic and Phasic Signal in Skeletal Muscle

Tanya R. Cully, Bradley S. Launikonis
2013 Biophysical Journal  
Vallmitjana 1 , Leif Hove-Madsen 2 .  ...  density Wednesday, February 6, 2013 607a Membrane Receptors & Signal Transduction II Board B280 A Method to Characterize Calcium Activity in Stimulated Cultures of Cardiac Myocytes Raul Benitez 1 , Alex  ... 
doi:10.1016/j.bpj.2012.11.3364 fatcat:rlsycc5ppnczblww27d2cwryma
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