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Metformin Attenuates Early-Stage Atherosclerosis in Mildly Hyperglycemic Oikawa-Nagao Mice

Akira Asai, Yuki Shuto, Mototsugu Nagao, Momoyo Kawahara, Teruo Miyazawa, Hitoshi Sugihara, Shinichi Oikawa
2019 Journal of atherosclerosis and thrombosis  
Here we assessed atherosclerotic lesion formation in newly established 2 mouse lines with different blood glucose levels (Oikawa-Nagao Diabetes-Prone [ON-DP] and -Resistant [ON-DR]) to evaluate the effect  ...  ., Oikawa-Nagao Diabetes-Prone (ON-DP) and -Resistant (ON-DR), formerly referred to as SDG-Prone and -Resistant, respectively [18] [19] [20] .  ... 
doi:10.5551/jat.48223 pmid:30971640 pmcid:PMC6927811 fatcat:cmybe3y4cncttmm5pbnhosiiqa

Rice Metabolomics

Akira Oikawa, Fumio Matsuda, Miyako Kusano, Yozo Okazaki, Kazuki Saito
2008 Rice  
Metabolomics is a recently developed technology for comprehensive analysis of metabolites in organisms. Plant metabolites that are produced for the growth, development, and chemical defense of plants against climatic alterations or natural predators are also useful to us as nutrients or medicines; hence, it is important to comprehend the amounts and varieties of plant metabolites. Besides providing an understanding of the metabolic state in plants under various circumstances, metabolomic
more » ... ues are applicable to the clarification of the functions of unknown genes by using natural variants or mutants of the target plants. Furthermore, a metabolomic approach might be useful in the breeding of crops, since valuable plant traits such as taste and yield are closely related to metabolic conditions. Here, we describe the methodology of metabolomics including sample extraction and preparation, metabolite detection, and data processing and analysis, and introduce the application of metabolomic studies to rice.
doi:10.1007/s12284-008-9009-4 fatcat:64wk3qkv4vhollmyj7722lwt64

Leptin production capacity determines food intake and susceptibility to obesity-induced diabetes in Oikawa–Nagao Diabetes-Prone and Diabetes-Resistant mice

Akira Asai, Mototsugu Nagao, Koji Hayakawa, Teruo Miyazawa, Hitoshi Sugihara, Shinichi Oikawa
2020 Diabetologia  
Here, we investigated the underlying mechanisms in determining spontaneous food intake in newly established Oikawa-Nagao Diabetes-Prone (ON-DP) and Diabetes-Resistant (ON-DR) mice.  ...  Although overeating is the major Akira Asai and Mototsugu Nagao contributed equally to this study.  ...  Recently, we established two mouse lines (closed colonies) with different susceptibilities to high-fat diet (HFD)-induced glucose intolerance, designated as Oikawa-Nagao Diabetes-Prone (ON-DP) and Oikawa-Nagao  ... 
doi:10.1007/s00125-020-05191-8 pmid:32561946 fatcat:epae5ex2wbakbepa4fr2wystcm

FoxO1 breaks diabetic heart

Mototsugu Nagao, Akira Asai, Shinichi Oikawa
2012 Journal of Diabetes Investigation  
Mototsugu Nagao, Akira Asai, Shinichi Oikawa* Division of Endocrinology and Metabolism, Department of Medicine, Nippon Medical School, Tokyo, Japan  ... 
doi:10.1111/jdi.12022 pmid:24843627 pmcid:PMC4019284 fatcat:yhgnhma3ejaz7o4qhuxkysob2q

20.7 Laser Termal Multilevel Recording -Structure Optimization-

Shigeru Oikawa, Akira Morinaka
1984 ITE Technical Report  
讃期 20 . 7 Laser Thermal Multilevel Recording − Structure Optimization − Shigeru O 亘 kawa , Akira Morinaka NiPpon Telegraph & Telephone Publlc Corp . 1barakl Japan SID Japan で 報 告 し た ,感 熱 発 色 光 記 録 媒 体  ... 
doi:10.11485/tvtr.8.14_39 fatcat:afmeidp3rbgf5ezmumslleyhbe

Bowman's layer encystment in cases of persistent Acanthamoeba keratitis

Akira Kobayashi, Yokogawa, Yamazaki, Sugiyama, Oikawa, Tokoro, Ishibashi
2012 Clinical Ophthalmology  
The purpose of this study was to report Acanthamoeba encystment in Bowman's layer in Japanese cases of persistent Acanthamoeba keratitis (AK). Methods: Laser confocal microscopic images of the cornea were obtained in vivo from 18 consecutive eyes from 17 confirmed AK patients. Retrospectively, 14 cases treated over 4 months were categorized as a nonpersistent group and three cases that required prolonged therapy for more than 6 months were categorized as a persistent group. Clinical outcomes
more » ... ed on final best-corrected visual acuity were retrospectively analyzed, and selected confocal images were evaluated qualitatively for abnormal findings. Results: The final best-corrected visual acuity was significantly lower (P , 0.01) for patients in the persistent group compared with that in the nonpersistent group. At the initial visit, in vivo confocal microscopy demonstrated Acanthamoeba cysts exclusively in the epithelial layer in both the nonpersistent group (80%) and the persistent group (100%). At a subsequent follow-up visit, numerous Acanthamoeba cysts were observed in the epithelial cell layer and in Bowman's layer in all patients with persistent AK, but Acanthamoeba cysts were undetectable in all cases with nonpersistent AK tested. Conclusion: Invasion of cysts into Bowman's layer was characteristically observed in patients with persistence of AK. This finding suggests that invasion of Acanthamoeba cysts into Bowman's layer may be a useful predictor for a persistent clinical course.
doi:10.2147/opth.s34695 pmid:22927735 pmcid:PMC3422145 fatcat:tshe34vqwfgopejydzhyn5rhzq

Fat Intake and Type 2 Diabetes
脂肪摂取と2型糖尿病

Mototsugu NAGAO, Akira ASAI, Hitoshi SUGIHARA, Shinichi OIKAWA
2015 Oleoscience  
doi:10.5650/oleoscience.15.69 fatcat:4gjytg2e5rgdxpca3dw56ga3em

Ex vivo laser confocal microscopy findings of cultured Acanthamoeba trophozoites

Akira Kobayashi, Tokoro, Yamazaki, Yokogawa, Sugiyama, Ishibashi, Oikawa
2012 Clinical Ophthalmology  
The purpose of the current study was to investigate ex vivo laser confocal microscopic findings of cultured Acanthamoeba trophozoites obtained from Acanthamoeba keratitis patients. Eight cultured samples of Acanthamoeba trophozoites from eight eyes of seven patients (mean age, 26.9 years; age range, 18-52 years) were used. Seven samples were from corneal scrapings of Acanthamoeba keratitis patients and one sample was from the solution in a soft contact lens case. Ex vivo laser confocal
more » ... y was performed to qualitatively evaluate the shape and degree of light reflection of the living Acanthamoeba trophozoites. Ex vivo laser confocal microscopy demonstrated highly reflective, high-contrast Acanthamoeba trophozoites with no walls (mean size, 25.4 μm; range, 17.1-58.5 μm). The shapes of the trophozoites were highly pleomorphic, and some showed characteristic acanthopodia by laser confocal microscopy. Ex vivo laser confocal microscopy was effective in demonstrating cultured Acanthamoeba trophozoites of various shapes and sizes. The observations of the current study may be helpful when similar structures are identified under in vivo conditions.
doi:10.2147/opth.s35258 pmid:22973090 pmcid:PMC3430162 fatcat:hpvu2xgbczfz5dj3o52pno3ijq

Arabidopsis Metabolome Analysis Using Infusion ESI FT-ICR/MS

Reiko Motohashi, Masakazu Satou, Fumiyoshi Myouga, Akira Oikawa, Daisaku Ohta
2015 Bio-protocol  
(Oikawa et al. 2006). Samples are infused using a Harvard syringe pump mode 22 at a flow rate of 0.5 to 1.0µL/min through a 100 µL Hamilton syringe.  ... 
doi:10.21769/bioprotoc.1463 fatcat:tyin2jvmgfcjxf6nlgk46eeiqm

ppGpp functions as an alarmone in metazoa [article]

Doshun Ito, Hinata Kawamura, Akira Oikawa, Yuta Ihara, Toshio Shibata, Nobuhiro Nakamura, Tsunaki Asano, Shun-Ichiro Kawabata, Takashi Suzuki, Shinji Masuda
2019 biorxiv/medrxiv   pre-print
Guanosine 3',5'-bis(pyrophosphate) (ppGpp) functions as a second messenger in bacteria to adjust their physiology in response to environmental changes. In recent years, the ppGpp-specific hydrolase, metazoan SpoT homolog-1 (Mesh1), was shown to have important roles for growth under nutrient deficiency in Drosophila melanogaster. Curiously, however, ppGpp has never been detected in animal cells, and therefore the physiological relevance of this molecule, if any, in metazoans has not been
more » ... hed. Here, we report the detection of ppGpp in Drosophila and human cells and demonstrate that ppGpp accumulation induces metabolic changes, cell death, and eventually lethality in Drosophila. Our results provide the first evidence of the existence and function of the ppGpp-dependent stringent response in animals.
doi:10.1101/2019.12.16.878942 fatcat:e76pb6yqrffuhav5ksqy4jhw4i

Preparation of pure lipid hydroperoxides

Daigo Ibusuki, Kiyotaka Nakagawa, Akira Asai, Shinichi Oikawa, Yuichi Masuda, Toshihide Suzuki, Teruo Miyazawa
2008 Journal of Lipid Research  
Masato Oikawa 375 (Graduate School of Life Sciences, Tohoku University) for helpful discussions about 376 reaction mechanism of LOOH with MxP, and Mr.  ... 
doi:10.1194/jlr.d800034-jlr200 pmid:18641373 fatcat:bm2zouhxxbattpzx2jzmrrw6kq

Coagulation factors and fibrinolytic activity in obstructive jaundice

Michio MITO, Akira TAMAKI, Akio KAWAMURA, Isao SAITO, Eiichi IKEDA, Iwao OIKAWA
1975 Blood & Vessel  
doi:10.2491/jjsth1970.6.199 fatcat:odilvmtwaffntdlkb3oer63wke

Fat intake and the development of type 2 diabetes [Review]

Mototsugu Nagao, Akira Asai, Hitoshi Sugihara, Shinichi Oikawa
2015 Endocrine journal  
The increase in the number of patients with diabetes has become a worldwide healthcare issue, with numbers predicted to reach approximately 600 million by 2035. In Asia-Pacific region, the prevalence of type 2 diabetes has increased dramatically in recent decades, of which the major causes are believed to be modern lifestyle changes, e.g., Western dietary pattern and reduced physical activity, on their genetic basis of lower insulin secretory capacity. Particularly, in East Asian countries, the
more » ... amount of fat intake has increased nearly three-fold over this half of century; dietary fat appears to be the major culprit of type 2 diabetes pandemic in East Asia. However, convincing evidence has not yet been provided as to whether high-fat diet causes type 2 diabetes in epidemiological cohort studies. Here, we summarize clinical studies regarding fat intake and type 2 diabetes, and animal studies on high-fat diet-induced diabetes including our recent works on the novel mouse lines (selectively bred diet-induced glucose intolerance-prone [SDG-P] and -resistant [SDG-R]) to address the etiology of high-fat diet-induced diabetes. These epidemiological and experimental findings would provide further insight into the etiology of type 2 diabetes under the modern nutritional environment, namely in the context of increased fat intake.
doi:10.1507/endocrj.ej15-0055 pmid:25924665 fatcat:gucdz4jrifgilgut5cibuwtdxa

Application Studies Using Metabolomics
メタボロミクスの応用研究

Akira OIKAWA, Fumio MATSUDA, Daisuke SAIGUSA
2017 Journal of the Mass Spectrometry Society of Japan  
doi:10.5702/massspec.s17-52 fatcat:2upgm7l2f5fltdduae3einzit4

Dry Development of Deep UV Resist with RF Plasma for Fine Pattern Lithography
高周波放電プラズマによるDeep UVレジストの微細パターン乾式現像法

Hisashi NAKANE, Wataru KANAI, Akira YOKOTA, Isamu HIJIKATA, Akira UEHARA, Setsuko OIKAWA, Minoru TSUDA
1981 Oyobuturi  
doi:10.11470/oubutsu1932.50.145 fatcat:fk4vexu44jghrem7u3jf5zn42u
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