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Nippon Jibiinkoka Gakkai Kaiho
経皮経食道胃管挿入術 (PTEG) の手技と工夫
経皮経食道胃管挿入術 (PTEG) の手技と工夫
Nippon Jibiinkoka Gakkai Kaiho
In eukaryotic nuclei, a number of phase-separated nuclear bodies (NBs) are present. RNA polymerase II (Pol II) is the main player in transcription and forms large condensates in addition to localizing at numerous transcription foci. Cajal bodies (CBs) and histone locus bodies (HLBs) are NBs that are involved in transcriptional and post-transcriptional regulation of small nuclear RNA and histone genes. By live-cell imaging using human HCT116 cells, we here show that Pol II condensates (PCs)doi:10.1101/2020.12.25.424380 fatcat:wuynzh4odfh47pcgapiscijb6m
more »... ated near CBs and HLBs, and the number of PCs increased during S phase concomitantly with the activation period of histone genes. Ternary PC-CB-HLB associates were formed via three pathways: nucleation of PCs and HLBs near CBs, interaction between preformed PC-HLBs with CBs, and nucleation of PCs near preformed CB-HLBs. Coilin knockout increased the co-localization rate between PCs and HLBs, whereas the number, nucleation timing, and phosphorylation status of PCs remained unchanged. Depletion of PCs did not affect CBs and HLBs. Treatment with 1,6-hexanediol revealed that PCs were more liquid-like than CBs and HLBs. Thus, PCs are dynamic structures often nucleated following the activation of gene clusters associated with other NBs.
Nippon Jibiinkoka Tokeibugeka Gakkai Kaiho(Tokyo)
森 尚彫 ＊， ＊＊ ，山口 忍 ＊， ＊＊＊ ，石田 愛 ＊ ，近藤香菜子 ＊ ，岡野 高之 ＊ ，伊藤 壽一 ＊＊＊＊ ，大森 孝一 ＊ ， 山本 典生 ＊ Naoe Mori ＊， ＊＊ , Shinobu Yamaguchi ＊， ＊＊＊ , Ai Ishida ＊ , Kanako Kondo ＊ , Takayuki Okano ＊ , Juichi Ito ＊＊＊＊ , Koichi ...doi:10.3950/jibiinkotokeibu.124.12_1664 fatcat:urpd3kttench7b4cu4odbfgc6i
Protein ubiquitylation regulates diverse cellular processes via distinct ubiquitin chains that differ by linkage type and length. However, a comprehensive method for measuring these properties has not been developed. Here we describe a method for assessing the length of substrate-attached polyubiquitin chains, "ubiquitin chain protection from trypsinization (Ub-ProT)." Using Ub-ProT, we found that most ubiquitylated substrates in yeast-soluble lysate are attached to chains of up to sevendoi:10.1038/s41467-018-02869-x pmid:29410401 pmcid:PMC5802829 fatcat:3lvajclrhzeyrm3o7sfr3ibi2e
more »... in molecules. Inactivation of the ubiquitinselective chaperone Cdc48 caused a dramatic increase in chain lengths on substrate proteins, suggesting that Cdc48 complex terminates chain elongation by substrate extraction. In mammalian cells, we found that ligand-activated epidermal growth factor receptor (EGFR) is rapidly modified with K63-linked tetra-to hexa-ubiquitin chains following EGF treatment in human cells. Thus, the Ub-ProT method can contribute to our understanding of mechanisms regulating physiological ubiquitin chain lengths and composition.
Genes to Cells
In eukaryotic nuclei, a number of phase-separated nuclear bodies (NBs) are present. RNA polymerase II (Pol II) is the main player in transcription and forms large condensates in addition to localizing at numerous transcription foci. Cajal bodies (CBs) and histone locus bodies (HLBs) are NBs that are involved in transcriptional and post-transcriptional regulation of small nuclear RNA and histone genes. By live-cell imaging using human HCT116 cells, we here show that Pol II condensates (PCs)doi:10.1111/gtc.12840 pmid:33608942 fatcat:q3vzfp4qyjdxbhbqpzb3xkzd2y
more »... ated near CBs and HLBs, and the number of PCs increased during S phase concomitantly with the activation period of histone genes. Ternary PC-CB-HLB associates were formed via three pathways: nucleation of PCs and HLBs near CBs, interaction between preformed PC-HLBs with CBs, and nucleation of PCs near preformed CB-HLBs. Coilin knockout increased the co-localization rate between PCs and HLBs, whereas the number, nucleation timing, and phosphorylation status of PCs remained unchanged. Depletion of PCs did not affect CBs and HLBs. Treatment with 1,6-hexanediol revealed that PCs were more liquid-like than CBs and HLBs. Thus, PCs are dynamic structures often nucleated following the activation of gene clusters associated with other NBs. (187 words).
Mesothelioma is a highly malignant tumor that is primarily caused by occupational or environmental exposure to asbestos fibers. Despite worldwide restrictions on asbestos usage, further cases are expected as diagnosis is typically 20-40 years after exposure. Once diagnosed there is a very poor prognosis with a median survival rate of 9 months. Considering this the development of early pre clinical diagnostic markers may help improve clinical outcomes. Methodology: Microarray expression arraysdoi:10.1371/journal.pone.0025391 pmid:21984916 pmcid:PMC3184985 fatcat:nzewrxfwqvh2fogsqqckpk6yfe
more »... mesothelium and other tissues dissected from mice were used to identify candidate mesothelial lineage markers. Candidates were further tested by qRTPCR and in-situ hybridization across a mouse tissue panel. Two candidate biomarkers with the potential for secretion, uroplakin 3B (UPK3B), and leucine rich repeat neuronal 4 (LRRN4) and one commercialized mesothelioma marker, mesothelin (MSLN) were then chosen for validation across a panel of normal human primary cells, 16 established mesothelioma cell lines, 10 lung cancer lines, and a further set of 8 unrelated cancer cell lines. Conclusions: Within the primary cell panel, LRRN4 was only detected in primary mesothelial cells, but MSLN and UPK3B were also detected in other cell types. MSLN was detected in bronchial epithelial cells and alveolar epithelial cells and UPK3B was detected in retinal pigment epithelial cells and urothelial cells. Testing the cell line panel, MSLN was detected in 15 of the 16 mesothelioma cells lines, whereas LRRN4 was only detected in 8 and UPK3B in 6. Interestingly MSLN levels appear to be upregulated in the mesothelioma lines compared to the primary mesothelial cells, while LRRN4 and UPK3B, are either lost or down-regulated. Despite the higher fraction of mesothelioma lines positive for MSLN, it was also detected at high levels in 2 lung cancer lines and 3 other unrelated cancer lines derived from papillotubular adenocarcinoma, signet ring carcinoma and transitional cell carcinoma.
Investigation: Chizuko Hirama, Ai Kaiho-Soma, Ayaka Yamaguchi, Hiroko Kogure, Sonomi Takakuwa, Mina Ogawa. Methodology: Daichi Sadato, Chizuko Hirama, Ai Kaiho-Soma. ...doi:10.1371/journal.pone.0255257 pmid:34297770 pmcid:PMC8301613 fatcat:7zf4stc2u5btvevuzhcn7goipq
The 26S proteasome is a 2.5-MDa multisubunit protease complex that degrades polyubiquitylated proteins. Although its functions and structure have been extensively characterized, little is known about its dynamics in living cells. Here, we investigate the absolute concentration, spatio-temporal dynamics and complex formation of the proteasome in living cells using fluorescence correlation spectroscopy. We find that the 26S proteasome complex is highly mobile, and that almost all proteasomedoi:10.1038/ncomms4396 pmid:24598877 fatcat:yae5v2xxzrarblsugyvni7jgk4
more »... ts throughout the cell are stably incorporated into 26S proteasomes. The interaction between 19S and 20S particles is stable even in an importin-a mutant, suggesting that the 26S proteasome is assembled in the cytoplasm. Furthermore, a genetically stabilized 26S proteasome mutant is able to enter the nucleus. These results suggest that the 26S proteasome completes its assembly process in the cytoplasm and translocates into the nucleus through the nuclear pore complex as a holoenzyme.
Cap analysis of gene expression (CAGE) is a 59 sequence tag technology to globally determine transcriptional starting sites in the genome and their expression levels and has most recently been adapted to the HeliScope single molecule sequencer. Despite significant simplifications in the CAGE protocol, it has until now been a labour intensive protocol. Methodology: In this study we set out to adapt the protocol to a robotic workflow, which would increase throughput and reduce handling. Thedoi:10.1371/journal.pone.0030809 pmid:22303458 pmcid:PMC3268765 fatcat:a2hevyytynck5py2ij25jfblqm
more »... ted CAGE cDNA preparation system we present here can prepare 96 'HeliScope ready' CAGE cDNA libraries in 8 days, as opposed to 6 weeks by a manual operator.We compare the results obtained using the same RNA in manual libraries and across multiple automation batches to assess reproducibility. Conclusions: We show that the sequencing was highly reproducible and comparable to manual libraries with an 8 fold increase in productivity. The automated CAGE cDNA preparation system can prepare 96 CAGE sequencing samples simultaneously. Finally we discuss how the system could be used for CAGE on Illumina/SOLiD platforms, RNA-seq and fulllength cDNA generation.
Natural Language Processing
AUTHORS Kaiho is a student currently attending Sentinel Secondary in West Vancouver. ... A similar recommendation program that implements AI is RikoNet, RikoNet uses an auto-encoder to cluster and filter data to make predictions . ...doi:10.5121/csit.2021.112317 fatcat:nsvuiqejhjczbkt3k53gb6deza
AI SVM : support vector machine AI AI GRBAS Google TensorFlow GRBAS Apple Create ML Core ML AI iPhone GRBASZero GRBAS AI AI AI AI AI 124 418 2021 ... IgG4 DS IgG4 DS IgG4 135mg/dL IgG4 IgG4 IgG4 IgG4 IgG4 IgG4 CT MRI IgG4 DS IgG4 DS IgG4 DS IgG4 DS CPG CPG CPG CPG arterially perfused brainstem preparation 124 416 2021 AI ...doi:10.3950/jibiinkoka.124.414 fatcat:gu6exgbxgvg4lkejoyviuv7njm
vertigo jumbling de- mentia ADL ADL ADL 124 426 2021 QOL CT MRI Alzheimer's disease, AD Parkinson's disease, PD AD mild cognitive impairment, MCI MCI PD PD olfactory training AI ... Petti Review AD AD MCI minimal cognitive impairment F1 F5 shimmer AI AD MCI QOL Cruz JentoftAJ, Baeyens JP, Bauer JM, et al : Sarcopenia : European consensus on definition and diagnosis ...doi:10.3950/jibiinkoka.124.424 fatcat:wxalun6mlbefnidgbaxwtn6xem
AI AI 121 356 2018 QOL QOL phenotype endotype Th2 Th17 Th2 Toll ILC2 CT MRI Open Essence olfactory training olfactory training ISCOANA Acoustic Rhinometry AR AR position ... therapy TRT TEES 3D MRI CT MRI Auditory processing disorder APD iPS iPS iPS iPS iPS CCD head impulse test VEMP MRI MRI CT MRI 3D MRI Epley BPPV drug delivery system QOL AI ...doi:10.3950/jibiinkoka.121.353 fatcat:3w4byo4zejarzfsctnqw3km4qq
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