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Controlling Ligand Binding in Myoglobin by Mutagenesis

Federica Draghi, Adriana Erica Miele, Carlo Travaglini-Allocatelli, Beatrice Vallone, Maurizio Brunori, Quentin H. Gibson, John S. Olson
2001 Journal of Biological Chemistry  
A quadruple mutant of sperm whale myoglobin was constructed to mimic the structure found in Ascaris suum hemoglobin. The replacements include His(E7) 3 Gln, Leu(B10) 3 Tyr, Thr(E10) 3 Arg, and Ile(G8) 3 Phe. Single, double, and triple mutants were characterized to dissect out the effects of the individual substitutions. The crystal structures of the deoxy and oxy forms of the quadruple mutant were determined and compared with that of native Ascaris hemoglobin. Tyr(B10) myoglobin displays low O
more » ... affinity, high dissociation rate constants, and heterogeneous kinetic behavior, suggesting unfavorable steric interactions between the B10 phenol side chain and His(E7). In contrast, all mutants containing the Tyr(B10)/Gln(E7) pair show high O 2 affinity, low dissociation rate constants, and simple, monophasic kinetic behavior. Replacement of Ile 107 with Phe enhances nanosecond geminate recombination singly and in combination with the Tyr(B10)/Gln(E7)/Arg(E10) mutation by limiting access to the Xe4 site. These kinetic results and comparisons with native Ascaris hemoglobin demonstrate the importance of distal pocket cavities in governing the kinetics of ligand binding. The ϳ150-fold higher O 2 affinity of Ascaris hemoglobin compared with that for Tyr(B10)/Gln(E7)-containing myoglobin mutants appears to be the result of favorable proximal effects in the Ascaris protein, due to a staggered orientation of His(F8), the lack of a hydrogen bonding lattice between the F4, F7, and F8 residues, and the presence of a large polar Trp(G5) residue in the interior portion of the proximal heme pocket. More than 50 years ago, Davenport (1) showed that the ultrahigh affinity of Ascaris suum hemoglobin for O 2 is due to a remarkably small rate constant for O 2 dissociation (Յ0.01 s Ϫ1 ). In 1994, De Baere et al. (2) and Kloek et al. (3) suggested that the structural cause of the low dissociation rate was electrostatic stabilization of bound O 2 by two hydrogen bonds, a very strong one with Tyr(B10) and a weaker one with Gln(E7).
doi:10.1074/jbc.m109206200 pmid:11744723 fatcat:lfvfqklqlndc5ddvu3cmqqmrr4

The HIV-1 Integrase C-Terminal domain induces TAR RNA structural changes promoting Tat binding [article]

Cecilia Rocchi, Camille Louvat, Adriana Erica Miele, Julien Batisse, Christophe Guillon, Lionel Ballut, Daniela Lener, Matteo Negroni, Marc Ruff, Patrice Gouet, Francesca Fiorini
2021 bioRxiv   pre-print
Recent evidence indicated that HIV-1 Integrase (IN) binds genomic viral RNA (gRNA) playing a critical role in viral particle morphogenesis and gRNA stability in host cells. Combining biophysical and biochemical approaches we show that the C-terminal flexible 18-residues tail of IN acts as a sensor of the peculiar apical structure of trans-activation response element RNA (TAR), directly interacting with its hexaloop. We highlighted how the whole IN C-terminal domain, once bound to TAR, can
more » ... its structure assisting the binding of Tat, the HIV trans-activator protein, which finally displaces IN from TAR. Our results are consistent with the emerging role of IN in early stage of proviral transcription and suggest new steps of HIV-1 life cycle that can be considered as therapeutic targets.
doi:10.1101/2021.10.21.465253 fatcat:uhxbnch7m5aipnyyg7aikipki4

Acknowledgement to Reviewers of Foods in 2019

Foods Editorial Office Foods Editorial Office
2020 Foods  
Josep Merlino, Valentina Maria Mesnage, Robin Mhd Jalil, Abbe Maleyki Miao, Tianxin Miceli, Natalizia Micevski, Milena Michalska, Anna Michel, Frederick Michel, Maximilian Middendorf, Dana Miele  ...  Noordergraaf, Inge-Willem Nowak, Adriana Nowak, Agnieszka Nowak, Anna Nowak, Dorota Nunes, Cecilia Núñez Carmona, Estefanía Nunez, Oscar Nychas, George-John E. O'Keefe, Sean F.  ... 
doi:10.3390/foods9010107 fatcat:tdffddaeijfwzp6uk3q5s5tsai

Page 10879 of Psychological Abstracts Vol. 90, Issue 4 [page]

2003 Psychological Abstracts  
., 10465 McDowell, Christopher, 9615 McEachern, Adriana G., 10289 McElroy, Todd, 9765 McEvoy, Joseph P., 8847 McFarland, Christine, 10376 McFarlane, Alexander, 10228 McGarigle, Carol, 11073 McGee, Anna  ...  Mimi, 9597 XXXVil Miassy-Maljak, Nelly, 11433 Michaelis, Benjamin H., 10135 Michalak, Erin E., 10136 Michalski, Joseph H., 11185 Michelson, David, 10964, 10984 Mick, Eric, 10029 Midford, Richard, 11464 Miele  ... 

Acknowledgement to Reviewers of Cells in 2018

Cells Editorial Office
2019 Cells  
Michiels, Carine Mielcarek, Michal Miele, Adriana Erica Milavetz, Barry Miletta, Maria Millar, J. Cameron Miller, Robert H.  ... 
doi:10.3390/cells8010060 fatcat:uplolcg53rappa4peqn6umjkg4

Acknowledgement to Reviewers of Microorganismsin 2019

Microorganisms Editorial Office
2020 Microorganisms  
Midelet-Bourdin, Graziella Miele, Adriana E. Mierzejewska, Jolanta Mihai, Mara Mihalca, Andrei Daniel Milhano Santos, Fátima MILLEMANN, Yves Miller, Darlene Miller, Joanna L.  ...  Valiadi, Martha Valitutti, Francesco Vallance, Bruce Vallesi, Adriana Vallini, Giovanni Valls, © 2020 by the authors. Licensee MDPI, Basel, Switzerland.  ... 
doi:10.3390/microorganisms8020155 fatcat:mgitenfq2ffn5djbcdlgagk654

Acknowledgement to Reviewers of Crystals in 2019

Crystals Editorial Office
2020 Crystals  
Miele, Adriana Erica Mihai, Marcela Mikuśkiewicz, Marta Milanesio, Marco Milanova, Maria Mildren, Rich Milioto, Stefana Miller, Wolfram Miller, Reece G.  ...  Bialecka-Florjańczyk, Ewa Bigi, Adriana Bigourd, Damien Bikbaev, Rashid G. Bilal, Osama R.  ... 
doi:10.3390/cryst10010045 fatcat:xrqxe6asejahrdj2qyy55dagee


Léster Rodríguez, Vicerrector Académico, Humberto Ruiz, Vicerrector Administrativo, Mario Secretaria, Nancy Prado, Pedro Salinas, Jefe
2006 unpublished
Patricia Vit, Eunice Enriquez, Monika O Barth, Adriana H Matsuda, Ligia B.  ...  Para las abejas nativas de Venezuela, el concepto miel de panal se transforma en miel de botija. Su uso como alimento y medicamento data desde los Mayas.  ... 

Supplemental Material for Proceedings of the American Psychological Association, Incorporated, for the Legislative Year 2015: Minutes of the Annual Meeting of the Council of Representatives February 20–22, 2015, Washington, DC, and August 5 and August 7, 2015, Washington, DC, and Minutes of the February, June, August, and December 2015 Meetings of the Board of Directors

2016 American Psychologist  
Rosen, Jennifer Eve Rosenthal, Erica K. Ross, Cindy M.  ...  Michel, Cammy Anne Michel, Lorena Mickelson, Bryan Mickolus, Ciana Miele, David Miers, Erin Michelle Mikell, Christie Milano, Matthew J. Milhem, Erin C.  ... 
doi:10.1037/amp0000022.supp fatcat:eqfef43wi5b4lfjrylvttjxqo4

Acknowledgment to Reviewers of Foods in 2020

Foods Editorial Office Foods Editorial Office
2021 Foods  
Tropea Garzia, Giovanna Maria Daniela Tirelli, Antonio Trudo, Sabrina Tirloni, Erica Trujillo, Trystram, Gilles Todolí, José Luis Trzaskowska, Monika Toepel, Ulrike Tsafantakis, Nikolaos Tofalo  ...  Izabela Molin, Marianne Michalska, Anna Monaci, Linda Michel, Piotr Monago-Maraña, Olga Michel, Visalli Moniruzzaman, Mohammed Middendorf, Dana Monks, Jenifer Midmore, David Montaña, María Miele  ... 
doi:10.3390/foods10020238 pmid:33503802 fatcat:g6m3e4uitbaqnklbsl6kyqhsta

Acknowledgment to Reviewers of Microorganisms in 2020

Microorganisms Editorial Office Microorganisms Editorial Office
2021 Microorganisms  
Miele, Adriana E. McFeeters, Robert Mierzejewska, Jolanta McGill, Jodi Migheli, Quirico McGiven, John Miglietta, Maria Mcgregor, Neil Miglio, Arianna McInroy, John A.  ...  Valitutti, Francesco Verma, Ranjit Vallesi, Adriana Verma, Smriti Valm, Alex M.  ... 
doi:10.3390/microorganisms9020268 pmid:33525504 fatcat:hp6zrmsihfhtvaffyrbvr5wcze

Acknowledgment to Reviewers of Animals in 2020

Animals Editorial Office Animals Editorial Office
2021 Animals  
Feuerbacher, Erica Farouk, Mohammed Hamdy Feyera, Takele Farzan, Vahab Fidalgo, Miguel Fassani, Édison José Figueroa Millán, Julio V.  ...  Sakhawat Huo, Lijun Hostens, Miel Hussain, Hidayat Jewgenow, Katarina Laven, Richard Laarman, Anne Lavon, Yaniv Labroussaa, Fabien Lavrenčič, Andrej Lacham-Kaplan, Orly Lawrence, Catherine E.  ... 
doi:10.3390/ani11020313 pmid:33513681 fatcat:asbkefrlszfw3bvbvk74ccxkmy

Apuntes para la vida de Alfredo González Prada

Luis Alberto Sánchez
1946 Letras : Órgano de la Facultad de Letras y Ciencias Humanas  
Doña Adriana estaba en París.  ...  La Junta se hallaba en plena luna de miel con la Democracia y su hermana, la Libertad. El lenguaje de los discursos oficiales habría sido maratiano si no hubiese olido va a restauración.  ...  Esto es como la boca de Vemis, que decía Luciano: dientes capaces de morder pero miel bajo la lengua. No es posible vivir bajo la férula de los generales v doctores sudamericanos".  ... 
doi:10.30920/letras.12.33.1 fatcat:3agdvtbskbdk5jabcepyvo35yu

Acknowledgment to Reviewers of Nutrients in 2020

Nutrients Editorial Office Nutrients Editorial Office
2021 Nutrients  
Sosa, Erica Staal, Jens Sotek, Zofia Stabouli, Stella V. Soto, Esther Cuadrado Stachowska, Ewa Sotomayor, Camilo G.  ...  , Silvia Merkler, David Miguez, Patricia Merli, Manuela Mika, Adriana Merlino, Valentina Maria Mikhailov, Theresa A.  ... 
doi:10.3390/nu13020382 fatcat:dsp57pmq2bbmpic7mxscti4qoa

Acknowledgement to Reviewers of Nutrients in 2019

Nutrients Editorial Office
2020 Nutrients  
Micillo, Raffaella Micke, Oliver Miele, Luca Miele, Mara Mielenz, Manfred Mielgo, Juan Mielgo-Ayuso, Juan Mierzyński, Radzisław Míguez, Jesús M.  ...  Uddin, Golam Mezbah Uebanso, Takashi Ueda, Norishi Uehara, Mariko Ufnal, Marcin Ulatowski, Lynn Ullrich, Matthias Ullrich, Reiner Ulrich, Craig Umar, Shahid Ummarino, Simone Unger, Erica  ... 
doi:10.3390/nu12010273 fatcat:hc5qsecoqzhl3l3aa2eyhhljpq
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