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Ab-origin: an enhanced tool to identify the sourcing gene segments in germline for rearranged antibodies

Xiaojing Wang, Di Wu, Siyuan Zheng, Jing Sun, Lin Tao, Yixue Li, Zhiwei Cao
2008 BMC Bioinformatics  
Partitioning the functional antibody sequences to their sourcing germline gene segments is vital not only for understanding antibody maturation but also for promoting the potential engineering of the therapeutic  ...  In the adaptive immune system, variable regions of immunoglobulin (IG) are encoded by random recombination of variable (V), diversity (D), and joining (J) gene segments in the germline.  ...  Yeo WeeKiang for helping us improving the English writing, and the anonymous reviewers for their constructive comments. We also thank Dr. Wu Wei for fruitful and helpful discussion.  ... 
doi:10.1186/1471-2105-9-s12-s20 pmid:19091020 pmcid:PMC2638160 fatcat:daeylty7cvczzbpb2rfqb2jbye

Ontogeny of Recognition Specificity and Functionality for the Broadly Neutralizing Anti-HIV Antibody 4E10

Kathryn A. K. Finton, Della Friend, James Jaffe, Mesfin Gewe, Margaret A. Holmes, H. Benjamin Larman, Andrew Stuart, Kevin Larimore, Philip D. Greenberg, Stephen J. Elledge, Leonidas Stamatatos, Roland K. Strong (+1 others)
2014 PLoS Pathogens  
The process of antibody ontogeny typically improves affinity, on-rate, and thermostability, narrows polyspecificity, and rigidifies the combining site to the conformer optimal for binding from the broader  ...  The ontogeny of 4E10, an autoreactive antibody with unexpected combining site flexibility, was delineated through structural and biophysical comparisons of the mature antibody with multiple potential precursors  ...  Acknowledgments We would like to thank Tim Vanden Bos and Madeline LaPatra for assistance with protein production. Author Contributions  ... 
doi:10.1371/journal.ppat.1004403 pmid:25254371 pmcid:PMC4177983 fatcat:hjjydmof35aqjdvhtgzipmobcy

Abstract Book, XIII International Congress of Histochemistry and Cytochemistry ICHC2008, Imaging of Cell Dynamics, 23rd-27th August 2008, Medical University of Gdansk, Poland

FHC Redaction
2008 Folia Histochemica et Cytobiologica  
line (Ab) originated from the melanotic form by a spontaneous alteration, differing in many biological properties.  ...  In recent years, the molecular cytogenetic analysis is becoming an important tool to identify chromosomal alterations as well as oncogenes and tumor suppressor genes involved in cancer pathogenesis.  ...  methylation, which are crucial for the attainment of the final oocyte differentiation.Indirect immunofluorescence with an antibody to 5-methyl cytosine was carried out to evaluate and compare the degree  ... 
doi:10.5603/4388 doaj:cdebe86bf2604301b2401e99ae9a504c fatcat:7j6nazvgnjf2bhooy5awdzq7uy