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A systems chemical biology study of malate synthase and isocitrate lyase inhibition in Mycobacterium tuberculosis during active and NRP growth

Elebeoba E. May, Andrei Leitão, Alexander Tropsha, Tudor I. Oprea
2013 Computational biology and chemistry  
We explore consequences of disrupting the function of malate synthase (MS) and isocitrate lyase (ICL) during aerobic and hypoxic non-replicating persistence (NRP) growth by using the SCB method to identify  ...  In vitro studies of Mtb growth have identified changes in isocitrate lyase (ICL) and malate synthase (MS) that enable bacterial persistence under low oxygen and other environmentally limiting conditions  ...  Oprea and A. Leitao partially supported by a NIH U54 grant, award number U54-MH084690. A.  ... 
doi:10.1016/j.compbiolchem.2013.07.002 pmid:24121675 pmcid:PMC4010430 fatcat:rhobrdmvdjf2hprmnh2wgyvgr4

A Universal Stress Protein that Controls Bacterial Stress Survival in Micrococcus luteus

Spencer Havis, Abiodun Bodunrin, Jonathan Rangel, Rene Zimmerer, Jesse Murphy, Jacob D. Storey, Thinh D. Duong, Brandon Mistretta, Preethi Gunaratne, William R. Widger, Steven J. Bark
2019 Journal of Bacteriology  
UspA616 regulates malate synthase and isocitrate lyase, glyoxylate pathway enzymes important for chronic M. tuberculosis infection.  ...  Proteomics demonstrated statistically significant log-phase upregulation of glyoxylate pathway enzymes isocitrate lyase and malate synthase in ΔuspA616::kan M. luteus.  ...  ACKNOWLEDGMENTS We thank Amy Vollmer (Swarthmore College), James Sacchettini (Texas A&M University), James Oliver (UNC Charlotte), Steven Finkel (University of Southern California), and Dianne Newman (  ... 
doi:10.1128/jb.00497-19 pmid:31548273 pmcid:PMC6872200 fatcat:savv5bxmmjbxtlxtwcbayitjo4

Computational Systems Chemical Biology [chapter]

Tudor I. Oprea, Elebeoba E. May, Andrei Leitão, Alexander Tropsha
2010 Msphere  
of chemical biology, pharmacology and systems biology.  ...  There is a critical need for improving the level of chemistry awareness in systems biology.  ...  It has been observed that during anaerobic growth conditions isocitrate lyase (ICL) increases five-fold (Wayne and Sohaskey, 2001) .  ... 
doi:10.1007/978-1-60761-839-3_18 pmid:20838980 pmcid:PMC3547368 fatcat:vbm6llfmqve37fxzcejh3lfdre

Oxygen Modulates the Effectiveness of Granuloma Mediated Host Response to Mycobacterium tuberculosis: A Multiscale Computational Biology Approach

Cheryl L. Sershen, Steven J. Plimpton, Elebeoba E. May
2016 Frontiers in Cellular and Infection Microbiology  
Our study integrates a physiological model of oxygen dynamics in the extracellular space of alveolar tissue, an agent-based model of cellular immune response, and a systems biology-based model of Mtb metabolic  ...  Mycobacterium tuberculosis associated granuloma formation can be viewed as a structural immune response that can contain and halt the spread of the pathogen.  ...  Ong‚iro for providing review and commentary for this paper. We would also like to thank Jeffrey Sarlo in the High Performance Computing Center at the University of Houston for technical support.  ... 
doi:10.3389/fcimb.2016.00006 pmid:26913242 pmcid:PMC4753379 fatcat:bihoshhqanff7haqa7v6y45u3i

Dissecting Host-Pathogen Interactions in TB Using Systems-Based Omic Approaches

Khushboo Borah, Ye Xu, Johnjoe McFadden
2021 Frontiers in Immunology  
The causative agent, Mycobacterium tuberculosis (Mtb) is one of the most successful pathogens that can manipulate host cell environment for adaptation, evading immune defences, virulence, and pathogenesis  ...  Tuberculosis (TB) is a devastating infectious disease that kills over a million people every year.  ...  FUNDING This study was supported by the Biotechnology and Biological Sciences Research Council (BBSRC) grant, BB/V010611/1 United Kingdom. The funders have no role in preparation of this work.  ... 
doi:10.3389/fimmu.2021.762315 pmid:34795672 pmcid:PMC8593131 fatcat:rhbpy4o6wndilf4glv76j2vlcu

Latent Tuberculosis Infection: Myths, Models, and Molecular Mechanisms

N. K. Dutta, P. C. Karakousis
2014 Microbiology and Molecular Biology Reviews  
The aim of this review is to present the current state of knowledge on human latent tuberculosis infection (LTBI) based on clinical studies and observations, as well as experimental in vitro and animal  ...  a small population of nonreplicating, "dormant" bacilli, and that caseous granulomas are the haven for LTBI.  ...  The glyoxylate shunt comprises the sequential activities of isocitrate lyase (Icl) and malate synthase (GlcB).  ... 
doi:10.1128/mmbr.00010-14 pmid:25184558 pmcid:PMC4187682 fatcat:sxy72ceqgvaanbiiv7kos7jgpu

The Genome Analysis of the Human Lung-Associated Streptomyces sp. TR1341 Revealed the Presence of Beneficial Genes for Opportunistic Colonization of Human Tissues

Erika Corretto, Ana Catalina Lara, Lucie Kotrbová, František Lorenc, Kateřina Petříčková, Roman Grabic, Alica Chroňáková
2021 Microorganisms  
TR1341 was isolated from the sputum of a man with a history of lung and kidney tuberculosis, recurrent respiratory infections, and COPD.  ...  The presence of a type VII secretion system, distinct from the ones found in Mycobacterium species, suggests a different colonization strategy than the one used by other actinomycete lung pathogens.  ...  The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.  ... 
doi:10.3390/microorganisms9081547 pmid:34442631 pmcid:PMC8401907 fatcat:g5kmey7ea5awxigy55odcqhe3y

Parasite Survival and Disease Persistence in Cystic Fibrosis, Schistosomiasis and Pathogenic Bacterial Diseases: A Role for Universal Stress Proteins?

Priscilla Masamba, Abidemi Paul Kappo
2021 International Journal of Molecular Sciences  
Their ubiquitous nature in various organisms, as well as their augmentation during conditions of stress, is a clear indication of their direct or indirect importance in providing resilience against such  ...  Although a highly conserved group of proteins, the molecular and biochemical aspects of their functions are largely evasive.  ...  Conflicts of Interest: The authors declare no conflict of interests.  ... 
doi:10.3390/ijms221910878 pmid:34639223 fatcat:4dqzzq5gkrcs7ica7igxn3pimq

Biophysical studies of bacterial proteins; can we address antibiotic resistance? [article]

Subrata Paul, University Of Calgary, University Of Calgary, Hans Vogel
This may originate from a subtle change in the helix angles, required for the attachment of the acyl chain.  ...  Detailed biophysical studies of the Escherichia.coli ferric citrate binding PBP, FecB, revealed that it could form complexes with a wide variety of different tricarboxylic acid-iron complexes.  ...  Polyketide synthase (PKS) and Nonribosomal peptide synthetase (NRPS) systems are amongst the largest and most complicated multi-enzymes in nature 47 .  ... 
doi:10.11575/prism/25138 fatcat:qzmqmv6povcixku5or2r3kruxa


Md Hassan
Isocitrate lyase is one of the crucial enzyme of mycobacterium tuberculosis which is involved in catalysing glyoxylate shunt and provides help in persistent infection of mycobacterium under stressed condition  ...  Alteration in salt concentration leads in to fibriller aggregate formation by Mycobacterium Isocitrate Lyase The domain architecture of DnaB consists of an Nterminal regulatory domain (NTD) separated by  ...  Secto r 3 9-A, Ch andiga rh -1 60 03 6, E-ma il: Mycobacterium tuberculosis (Mtb), the principal etiological agent of tuberculosis in humans, is reported to infect more than  ... 

Biosynthesis and heterologous production of polyunsaturated fatty acids from myxobacteria [article]

Katja Gemperlein, Universität Des Saarlandes, Universität Des Saarlandes
of PUFA synthase from Sorangium cellulosum with a new product spectrum Chapter 3: 82 Heterologous expression of a myxobacterial PUFA biosynthetic pathway in Escherichia coli and Pseudomonas putida and  ...  2 Introduction 3 Chapter 1: 25 Polyunsaturated fatty acid biosynthesis in myxobacteria: Different PUFA synthases and their product diversity Chapter 2: 58 Discovery and characterization of a second type  ...  Helmholtz Centre for Infection Research) for fruitful discussions, Andrea Rademacher (previously InterMed Discovery GmbH) for strain cultivation, Susanne Schneiker-Bekel (CeBiTec) for genome sequencing, and  ... 
doi:10.22028/d291-23027 fatcat:uvhauvwyczg6lorntu43xsvu3a