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A protein interaction atlas for the nuclear receptors: properties and quality of a hub-based dimerisation network

Gregory D Amoutzias, Elgar E Pichler, Nina Mian, David De Graaf, Anastasia Imsiridou, Marc Robinson-Rechavi, Erich Bornberg-Bauer, David L Robertson, Stephen G Oliver
2007 BMC Systems Biology  
We also compare the evolution, topology and properties of the nuclear receptor network with the hub-based dimerisation network of the bHLH transcription factors in order to identify both unique themes  ...  of the nuclear receptor interaction network in humans.  ...  Work on protein interactions in DLR's and SGO's groups is supported by the BBSRC and by a Beacon Award (to SGO) from the UK Department of Trade & Industry.  ... 
doi:10.1186/1752-0509-1-34 pmid:17672894 pmcid:PMC1971058 fatcat:wbn7p5ii3rflddupj7pr5p6koy

BNA Festival of Neuroscience 2019

2019 Brain and Neuroscience Advances  
For the betweenness centrality, only ROIs at 2.5 SD > mean, identified as network hubs, were inspected. This was done for the entire population and the subgroup.  ...  Methods: An adolescent subset (N=976, age= 14.45 ± 0.45) of the IMAGEN consortium (Schumann, Loth et al. 2010) was identified based on imaging quality control and completion of neuropsychological assessments  ...  Methods: We are using purified proteins to investigate the properties of PICK1 dimerisation in a reduced system.  ... 
doi:10.1177/2398212819855490 fatcat:y4b5r7rpdnblzoxvb2crpzqhee

LC-MS/MS measurement of parathyroid hormone PTH (1-34): Use in studying oral PTH (1-34) administration and possible diagnostic application in pseudohypoparathyroidism

Sulaiman Al-Riyami, Jonathan Tang, Hillel Galitzer, William Fraser
2017 Endocrine Abstracts  
The abstracts submitted were marked by the Abstract Marking panel selected by the Programme Committee.  ...  A Microarray Assay for Real-time Co-regulator-Nuclear Receptor Interaction (MARCoNI) of 20b-DHF as a GR or MR ligand supported these findings.  ...  and arylhydrocarbon receptor-interacting protein (AIP)) implicated in the aetiology of parathyroid and pituitary tumours was undertaken.  ... 
doi:10.1530/endoabs.50.p042 fatcat:mlqefg6jxbevjcab3eid4sfxpy

Abstract Book: ISEV2017

2017 Journal of Extracellular Vesicles  
The isolated EVs were analysed with nanoparticle tracking analysis and transmission electron microscopy, and characterised for the presence of protein markers with western blotting and ELISA assays.  ...  The study protocol and use of all material was approved by local ethical committees and comply with the Declaration of Helsinki.  ...  HuRI interactions within the EV proteome showed 6 clusters, each centred on a well-connected "hub" protein.  ... 
doi:10.1080/20013078.2017.1310414 pmid:29046763 pmcid:PMC5637785 fatcat:ejbhnmhfqbeuronzsj47flas6u

Pros and Cons of Endoscopic Surgery [chapter]

Francesca Del Bon, Alberto Paderno, Alberto Schreiber, Nausica Montalto, Cesare Piazza, Piero Nicolai
2016 Critical Issues in Head and Neck Oncology  
The estimation of a HR of about 0.9 from network meta-analysis for a comparison of SFX-CRTX vs.  ...  Binding of ligand to the extracellular ligand-binding domain leads to receptor dimerisation and activation of the kinase function in the intracellular domain.  ...  nursing contact and availability The greatest issue of all dying patients is maintaining quality of life and ensuring a 'good death' for people whom we have cared for, often for many years.  ... 
doi:10.1007/978-3-319-42909-0_7 fatcat:56hvh3zyingmlpyyh75q5hylo4

Metabolic reprogramming to optimise breast cancer treatment

Amy L. Barber
network and a kinetic cell cycle model.  ...  In conclusion, a systems biology approach can enhance and accelerate the identification of novel drug targets and aid development of network targeting treatments.  ...  Develop a dynamic, multi-scale computational model of a breast cancer cell, based on a genome-scale metabolic network and incorporating select nuclear receptors and a kinetic model of the cell cycle   ... 
doi:10.15126/thesis.00853293 fatcat:yx2uioj27vb2hbz55k4fwb2hve

Ras Mapk Growth Effectors in Drosophila

Sebastian I. Florescu
Although not completely understood, studies have revealed multiple facets of this mechanism involving release from cytoplasmic anchors, phosphorylation and dimerisation, nuclear retention and a novel nuclear  ...  The Ras-Erk Mapk module in drosophila. Mitogens and growth factors bind to RTK (receptor tyrosine kinase) receptors triggering receptor dimerisation and activation.  ... 
doi:10.11588/heidok.00027050 fatcat:by2wizncdjbxrheflfbikbi3y4

Ubiquitin-dependent regulation of the WNT cargo protein EVI/WLS

Lucie Magdalena Wolf
receptors (which can be activated upstream of the phosphatidylinositol signal pathway) and enzyme-linked receptors, such as the epidermal growth factor receptor (EGFR), which dimerises upon ligand binding  ...  Additionally, the amino acid sensorindependent (Asi) complex controls protein quality at the nuclear envelope, which is continuous with the ER membrane.  ... 
doi:10.11588/heidok.00029362 fatcat:5qltv6czvfbifjqaq6zlb5ylve

Investigating the role of nuclear encoded mitochondrial genes in the onset of type 2 diabetes

Hannah Elizabeth Billaux Maude, Toby Andrew, Derek Huntley, Marjo-Riitta Jarvelin, Medical Research Council (Great Britain)
Here, a genetic study design is used to investigate the potential role of T2D-associated genetic risk loci (T2D loci) in disrupting mitochondrial function through the altered expression of nuclear- encoded  ...  and implicated a number of pathways in the inherited risk of T2D, including the relevant pathway of branched-chain amino acid catabolism.  ...  T2D is associated with missense mutations in ADIPOQ, which encodes the adipokine adiponectin, and PPARG, which encodes the nuclear receptor peroxisome proliferator-activated receptor-γ (PPARγ) and is highly  ... 
doi:10.25560/90969 fatcat:3urbfc3huneh3b3jwhuwoyzoey

Evaluating the efficacy of HTL001, a cell penetrating peptide that targets the HOX/PBX interaction in ovarian cancer

Giulia Falgari
HXR9 is a cell penetrating peptide capable of disrupting the interaction between HOX genes and their co-factor PBX to prevent the activation of downstream HOX target genes (oncogenes?)  ...  The limited cytotoxicy of HTL001 on ovarian cancer precision cut slices prompted us to investigate another strategy for drug delivery and the approach to insert multiple copies of HTL001 into a viral vector  ...  For those who are not familiar with the technical terminology, Cytoscape is a programme to draw functional protein association networks or to import existing networks from another proteinprotein interaction  ... 
doi:10.15126/thesis.00853230 fatcat:fdhwynan2bhr7p3wnfesw55c5u

Hypothalamic Nutrient Sensing

Nicholas John Heeley, Apollo-University Of Cambridge Repository, Apollo-University Of Cambridge Repository, Mark L Evans, Clemence S Blouet
PhosphoTRAP, and subsequent pharmacological studies identified a T Type calcium channel may be a marker for leucine sensing cells.  ...  A subpopulation of AgRP neurons express glucokinase, and some AgRP neurons are glucose inhibited, but the role of glucokinase in these neurons has not been characterised.  ...  Growth Response 4, Nupr1 -Nuclear Protein 1, Transcriptional Regulator, Srf -Serum response factor, Nr4a1 -Nuclear Receptor Subfamily 4 Group A Member 1, Cxcl1chemokine ligand 1.  ... 
doi:10.17863/cam.18622 fatcat:veo7rfs5xndandaqe7znbpb3ky

Central regulation of energy homeostasis and the reproductive axis

Charlotte Katie Boughton, Gavin Bewick, Kevin Murphy
These studies have elucidated the biological effects of alarin in the regulation of appetite and the HPG axis, and identified a role for the melanocortin system in regulating the central reward circuitry  ...  The ventral tegmental area (VTA) is the origin of the mesolimbic dopamine pathway, which mediates the rewarding properties of palatable food.  ...  Both the GR and the MR are nuclear receptors.  ... 
doi:10.25560/6183 fatcat:xb6tgpoapjg7xke3z77qos3f4q

Deciphering the transcriptional response of saccharomyces cerevisiae to perturbations of lipid metabolism and graded endoplasmic reticulum stress

Felix Reinhard Jonas, Guy-Bart Stan, Karen Polizzi
Secondly, the developed reporter proteins, a fluorescent sphingolipid (SL) intermediate and classical molecular biology techniques were used to investigate the interaction of the signaling pathways, which  ...  Thirdly, the adaptation to low ER stress was studied with a reporter protein for HAC1 and established fluorescent labels.  ...  It forms a large network that extends from the nucleus, where it blends with the nuclear membrane (i.e. perinuclear ER), to other cellular organelles and the plasma membrane (i.e. cortical ER).  ... 
doi:10.25560/68683 fatcat:rz4f3o7zinaq5ofg3q67m3ykxa