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High performance liquid chromatography/video fluorometry. Part I: Instrumentation

M. P. Fogarty, D. C. Shelly, I. M. Warner
1981 HRC Journal of High Resolution Chromatography  
The application of new technologies has extended the multicomponent detection capabilities of HPLC detectors.  ...  These plots represent either the total emission (as a function of excitation wavelength) or the total excitation (as a function of emission wavelength) versus time.  ... 
doi:10.1002/jhrc.1240041105 fatcat:x2lgeriri5fspnq2lotlnkck2q

Global analysis of steady-state polarized fluorescence spectra using trilinear curve resolution

G.R. Phillips, S. Georghiou
1993 Biophysical Journal  
The normalized excitation and emission vectors are combined with the total absorption spectrum of the multicomponent mixture to compute absolute absorption and emission spectra.  ...  This methodology is applicable to mixtures the components of which have linearly independent excitation and emission spectra and distinct anisotropies.  ...  The technical assistance of Mr. Thomas D. Bradrick is gratefully acknowledged. This work was supported in part by research grant GM38236 from the National Institutes of Health.  ... 
doi:10.1016/s0006-3495(93)81127-5 pmid:8218915 pmcid:PMC1225793 fatcat:parhg7egezep7bmce7dnk7zkdu

Recent Advances in Multicomponent Fluorescence Analysis

Isiah M. Warner, Linda B. McGown, Gary D. Christian
1982 C R C Critical Reviews in Analytical Chemistry  
The technique of seiective modulation was demonstrated to be a new and interesting approach to analysis of simple multicomponent Mixtures.  ...  The video fluorimeter should continue to provide new avenues for multicomponent fluorescence analysis. However. a major difficulty of this system is in the handling of the large data base generated.  ... 
doi:10.1080/10408348208542752 fatcat:czhan3jwrngifbs4kgyonhze7e

Recent Advances in Multicomponent Fluorescence Analysis

Isiah M. Warner, Linda B. McGown, Gary D. Christian
1982 Critical reviews in analytical chemistry  
The technique of seiective modulation was demonstrated to be a new and interesting approach to analysis of simple multicomponent Mixtures.  ...  The video fluorimeter should continue to provide new avenues for multicomponent fluorescence analysis. However. a major difficulty of this system is in the handling of the large data base generated.  ... 
doi:10.1080/10408348208085606 fatcat:diyt4f55nvbcdkmu65hlac3ure

Multiparametric Detection In Modern Luminescence Spectrometry

Chu-Ngi Ho, Mae E. Rollie, Isiah M. Warner
1983 Optical Engineering: The Journal of SPIE  
The Emission-Excitation Matrix (EED) The selectivity of luminescence analysis can be greatly enhanced if one can simultaneously examine a series of excitation and mission spectra of a sample.  ...  Even with this Inherent selectivity and specificity, some compounds will still possess very similar excitation and emission spectra such that spectroscopic resolution is not possible.  ...  Ac kmowledgeieuts This work was supported in part by $rants from the Office of Naval Research and the National Science Foundat ion (CUE 6 210886).  ... 
doi:10.1117/12.7973198 fatcat:tlqicf4b2zcdhgsyrpmzowdl4e

Mechanochemical Syntheses of Isostructural Luminescent Cocrystals of 9-Anthracenecarboxylic Acid with two Dipyridines Coformers

Torvid Feiler, Biswajit Bhattacharya, Adam A.L. Michalchuk, Vincent Schröder, Emil List-Kratochvil, Franziska Emmerling
2020 Crystals  
While the O–H···N hydrogen bonds adopts a primary role in the stabilization of the cocrystal phases, the C–H···O hydrogen bonding interactions appear to play a significant role in guiding the three-dimensional  ...  With the aim of discovering new functional luminescent materials, new cocrystals of 9-anthracene carboxylic acid (ACA) were prepared with two different dipyridine coformers: 1,2-bis(4-pyridyl)ethylene  ...  Conflicts of Interest: The authors declare no competing financial interests.  ... 
doi:10.3390/cryst10100889 fatcat:43jduo4e4ng6rjs6nsfvul44hm

High performance liquid chromatography/video fluorometry. Part II: Applications

D. C. Shelly, M. P. Fogarty, I. M. Warner
1981 HRC Journal of High Resolution Chromatography  
While the excitation spectra are similar, the emission spectra are very different, not only in terms of structure but also with respect to emission maxima.  ...  Imaging detectors, such as the silicon vidicon have been used to monitor the absorption [5] , fluorescence [6] and combined excitation and emission spectra [7] [8] [9] of LC effluents.  ... 
doi:10.1002/jhrc.1240041204 fatcat:jk6osw5dpfg2dczpaqos3k3hjq

Characterization of the effect of peak shifts on the performance of the Kalman filter in multicomponent analyses

Gail Hartmann Webster, Todd L. Cecil, Sarah C. Rutan
1989 Journal of Chemometrics  
There are a wide variety of curve resolution approaches which may be employed to obtain quantitative information from overlapped spectra, but none of these methods offers a convenient means for correction  ...  The approach described for two-component Gaussian systems has not yet been applied to multicomponent PAH systems; however, the one-component PAH results described above indicate that this general approach  ... 
doi:10.1002/cem.1180030105 fatcat:j5v5nx7nlze75llmx7fom6zmtm

Three-dimensional synchronous fluorescence spectrometry for the analysis of three-component alkaloid mixtures

F Sanchez
1990 Talanta: The International Journal of Pure and Applied Analytical Chemistry  
A three-dimensional plot of both emission-excitation and synchronous spectra obtained by a new software program provides additional information for optimizing instrumental parameters.  ...  Linear, normal and derivative calibration graphs are established in the ng/ml range. A statistical analysis of the results and their application to synthetic and natural samples is given.  ...  derivative approach for analysis of multicomponent samples prevents cross-interferences arising from absorption and/or emission by the other components; this is done by manipulation of the solvent composition  ... 
doi:10.1016/0039-9140(90)80199-p fatcat:hdcitvukxzhtzkqoftiw3gqwva

Near-infrared-emissive polymersomes: Self-assembled soft matter for in vivo optical imaging

P. P. Ghoroghchian, P. R. Frail, K. Susumu, D. Blessington, A. K. Brannan, F. S. Bates, B. Chance, D. A. Hammer, M. J. Therien
2005 Proceedings of the National Academy of Sciences of the United States of America  
a large window of the visible and NIR spectra (600-950 nm).  ...  Furthermore, the total fluorescence emanating from the assemblies gives rise to a localized optical signal of sufficient intensity to penetrate through the dense tumor tissue of a live animal.  ...  The animal was then killed, and the tumor was subsequently imaged ex vivo with a high-resolution (80 ϫ 80 ϫ 10 m 3 ) 3D scanning optical imaging system (34) to corroborate the localization of the emissive  ... 
doi:10.1073/pnas.0409394102 pmid:15708979 pmcid:PMC549472 fatcat:u7dd2pe44bgfbilmeytl5midqi

Self-absorption in [C ii], 12CO, and Hi in RCW120. Building up a geometrical and physical model of the region

S. Kabanovic, N. Schneider, V. Ossenkopf-Okada, et al.
2021 Astronomy and Astrophysics  
The high spectral resolution of the heterodyne observations turns out to be essential in order to analyze the physical conditions, geometry, and overall structure of the sources.  ...  Two types of radiative transfer models were used to fit the observed [C II] and CO spectra.  ...  This study was based on observations made with the NASA/DLR Stratospheric Observatory for Infrared Astronomy (SOFIA).  ... 
doi:10.1051/0004-6361/202142575 fatcat:jat5afjn7zddvdmjvomasw7dd4

Color-blind fluorescence detection for four-color DNA sequencing

E. K. Lewis, W. C. Haaland, F. Nguyen, D. A. Heller, M. J. Allen, R. R. MacGregor, C. S. Berger, B. Willingham, L. A. Burns, G. B. I. Scott, C. Kittrell, B. R. Johnson (+2 others)
2005 Proceedings of the National Academy of Sciences of the United States of America  
Unlike emission wavelength-dependent approaches for identifying fluorescent species, the removal of the spectral component in PME confers a number of advantages including higher and normalized signals  ...  We present an approach called pulsed multiline excitation (PME) for measurements of multicomponent, fluorescence species and demonstrate its application in capillary electrophoresis for DNA sequencing.  ...  To characterize the degree of spectral overlap between the PME dyes, the detector was configured with a spectrophotometer to obtain the entire emission spectra for each dye-labeled primer by excitation  ... 
doi:10.1073/pnas.0501606102 pmid:15800037 pmcid:PMC556273 fatcat:odl5oo2jvveo7gqg27o23pu43y

Multiplexed bioluminescence microscopy via phasor analysis [article]

Zi Yao, Caroline K. Brennan, Lorenzo Scipioni, Hongtao Chen, Kevin Ng, Michelle Digman, Jennifer A. Prescher
2021 bioRxiv   pre-print
The merger of bioluminescence and phasor analysis provides a platform for routine, time-lapse imaging of collections of cellular features.  ...  Microscopic bioluminescence imaging has been historically challenging due to a lack of detection methods and easily resolved probes.  ...  Nano-lanterns, like other luciferases, exhibit broad emission spectra, complicating their resolution by color alone.  ... 
doi:10.1101/2021.06.18.448905 fatcat:lkk6miw4zbgdrcgwj5xwjr3e7a

Self-absorption in [CII], ^12CO, and HI in RCW120. Building up a geometrical and physical model of the region [article]

S. Kabanovic, N. Schneider, V. Ossenkopf-Okada, F. Falasca, R. Güsten, J. Stutzki, R. Simon, C. Buchbender, L. Anderson, L. Bonne, C. Guevara, R. Higgins (+10 others)
2021 arXiv   pre-print
With a two-layer multicomponent model, we then quantified the amount of warm background and cold foreground gas.  ...  There is a deficit of CO emission along the line-of-sight toward the center of the HII region which indicates that the HII region is associated with a flattened molecular cloud.  ...  This study was based on observations made with the NASA/DLR Stratospheric Observatory for Infrared Astronomy (SOFIA).  ... 
arXiv:2112.11336v1 fatcat:bsbfppmehfdebcxk7w7aqptf7u

Multiphoton fluorescence lifetime contrast in deep tissue imaging: prospects in redox imaging and disease diagnosis

V. Krishnan Ramanujan, Jian-Hua Zhang, Eva Biener, Brian Herman
2005 Journal of Biomedical Optics  
The prospects for this approach in metabolic redox imaging and disease diagnosis are discussed.  ...  Turbid tissues pose serious problems of strong absorption and scattering that make steady state fluorescence imaging methods less successful in imaging tissue layers deeper than a few tens of micrometers  ...  Some of these signals have significantly overlapping emission spectra, which makes it a challenging task to isolate different autofluorescence signals reliably.  ... 
doi:10.1117/1.2098753 pmid:16292944 fatcat:gsvw47fcpbad5ewmiixxvmesiq
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