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Cloning, Heterologous Expression, and Enzymological Characterization of Human Squalene Monooxygenase

Brian P. Laden, Yinzi Tang, Todd D. Porter
2000 Archives of Biochemistry and Biophysics  
The apparent K m for NADPH-cytochrome P450 reductase, the requisite electron transfer partner, is 14 nM.  ...  As no data were available for humans, the ability of a number of tellurium and related elemental compounds to inhibit the recombinant human enzyme was examined.  ...  Glenn Prestwich and Ms. Hee-Kyoung Lee, University of Utah, for generous assistance in establishing the squalene monooxygenase assay and Dr.  ... 
doi:10.1006/abbi.1999.1629 pmid:10666321 fatcat:u65txrre3jgxlbd4n5sdxmfhkm

Age-Related Changes in Carcinogen Metabolism

Linda S. Birnbaum
1987 Journal of The American Geriatrics Society  
The cytochromes P450 are a family of hemo- proteins with overlapping substrate specificity.  ...  The situation in nonhuman primates suggests little change in the components of the hepatic monooxy- genase system.  ... 
doi:10.1111/j.1532-5415.1987.tb01319.x pmid:3540077 fatcat:v3xpifavxrg7tigtr24x4vxqmi

IsdG and IsdI, Heme-degrading Enzymes in the Cytoplasm ofStaphylococcus aureus

Eric P. Skaar, Andrew H. Gaspar, Olaf Schneewind
2003 Journal of Biological Chemistry  
IsdG and IsdI cleave the tetrapyrrol ring structure of heme in the presence of NADPH cytochrome P450 reductase, thereby releasing iron.  ...  Staphylococcus aureus requires iron for growth and utilizes heme as a source of iron during infection.  ...  Chuan He and Piotr Gornicki for reagents, help, and technical assistance. We would especially like to thank Dr. Michael Schmitt for providing all Corynebacterium strains and vectors.  ... 
doi:10.1074/jbc.m307952200 pmid:14570922 fatcat:4zxxwr7rz5fohag4ph3cpv6p3m

Yeast as a promising heterologous host for steroid bioproduction

Shanhui Xu, Yanran Li
2020 Journal of Industrial Microbiology & Biotechnology  
Sterols are essential components of cell membrane, and are the precursors for the biosynthesis of steroid hormones, signaling molecules, and defense molecules in the higher eukaryotes, which are of pharmaceutical  ...  , the challenge and the potential of using yeast as the bioproduction platform of various steroids from higher eukaryotes.  ...  Acknowledgements We thank Tiffany Chiu, Anqi Zhou, and Dr. Sheng Wu for their valuable feedback in the preparation of the manuscript.  ... 
doi:10.1007/s10295-020-02291-7 pmid:32661815 fatcat:eewrvseml5fpvodoaxnbdtufsq

Biodegradation of Chloroxylenol by Cunninghamella elegans IM 1785/21GP and Trametes versicolor IM 373: Insight into Ecotoxicity and Metabolic Pathways

Marta Nowak, Katarzyna Zawadzka, Janusz Szemraj, Aleksandra Góralczyk-Bińkowska, Katarzyna Lisowska
2021 International Journal of Molecular Sciences  
Identified intermediates were performed by dechlorination, hydroxylation, and oxidation reactions catalysed by cytochrome P450 enzymes and laccase.  ...  Its intensive application leads to the release of PCMX into the environment, which can have a harmful impact on aquatic and soil biotas.  ...  Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/ijms22094360 pmid:33921959 fatcat:syqmzowmxbdqzojisnli5yawum

Use of Heterologous Complementary DNA Array Screening to Analyze Bovine Oocyte Transcriptome and Its Evolution During In Vitro Maturation1

Rozenn Dalbiès-Tran, Pascal Mermillod
2003 Biology of Reproduction  
To our knowledge, this is the first report of gene expression profiling by this technology in the mammalian female germ cell.  ...  The relative abundance of most messenger RNAs appeared stable during IVM. However, 70 transcripts underwent a significant differential regulation (by a factor of at least two).  ...  Allan King for critical reading of the manuscript.  ... 
doi:10.1095/biolreprod.102.007872 pmid:12493721 fatcat:cvrn2frwinhovd7vjs7xcrdira

Flavoprotein monooxygenases, a diverse class of oxidative biocatalysts

W.J.H. van Berkel, N.M. Kamerbeek, M.W. Fraaije
2006 Journal of Biotechnology  
Furthermore, the biocatalytic potential of a selected number of flavoprotein monooxygenases is highlighted.  ...  During the last decades a large number of flavin-dependent monooxygenases have been isolated and studied.  ...  The specific type of oxygenation and selectivity depends on the shape and chemical nature of the active site of each specific monooxygenase.  ... 
doi:10.1016/j.jbiotec.2006.03.044 pmid:16712999 fatcat:jz5mdjuhm5gz5kuppjmonh27mi

Biotransformation and Induction: Implications for Toxicity, Bioaccumulation and Monitoring of Environmental Xenobiotics in Fish

Kevin M. Kleinow, Mark J. Melancon, John J. Lech
1987 Environmental Health Perspectives  
Exposure of fish to low levels of certain environmental contaminants has resulted in induction of specific monooxygenase activities and monitoring of such activities has been suggested as a means of identifying  ...  These reactions have been shown to affect the bioaccumulation, persistence, residue dynamics, and toxicity of select chemicals in fish.  ...  The significance of induction by select agents or lack of induction has not been fully realized on a mechanistic and practical basis.  ... 
doi:10.2307/3430418 fatcat:2vj4sua2qnehxpkuxyi27ibzm4

Biotransformation and induction: implications for toxicity, bioaccumulation and monitoring of environmental xenobiotics in fish

K M Kleinow, M J Melancon, J J Lech
1987 Environmental Health Perspectives  
Exposure of fish to low levels of certain environmental contaminants has resulted in induction of specific monooxygenase activities and monitoring of such activities has been suggested as a means of identifying  ...  These reactions have been shown to affect the bioaccumulation, persistence, residue dynamics, and toxicity of select chemicals in fish.  ...  The significance of induction by select agents or lack of induction has not been fully realized on a mechanistic and practical basis.  ... 
doi:10.1289/ehp.8771105 pmid:3297653 pmcid:PMC1474347 fatcat:bo4ef6mqqfezbgi73zvwnzxp3m

KEGG orthology-based annotation of the predicted proteome of Acropora digitifera: ZoophyteBase - an open access and searchable database of a coral genome

Walter C Dunlap, Antonio Starcevic, Damir Baranasic, Janko Diminic, Jurica Zucko, Ranko Gacesa, Madeleine JH van Oppen, Daslav Hranueli, John Cullum, Paul F Long
2013 BMC Genomics  
These models were used to search the predicted proteome of A. digitifera to establish complete genomic annotation.  ...  Here we present KEGG orthology-based annotation of the complete genome sequence of the scleractinian coral Acropora digitifera and provide the first comprehensive view of the genome of a reef-building  ...  of A. digitifera publically available without restriction of use.  ... 
doi:10.1186/1471-2164-14-509 pmid:23889801 pmcid:PMC3750612 fatcat:76ounlm75bervj5siyvqesowfm

Page 2303 of Bibliography of Agriculture. Annual Cumulative Indexes Vol. 59, Issue Cumulative Index [page]

Bibliography of Agriculture. Annual Cumulative Indexes  
microsomal monooxy- genase systems. 1184943 Differential induction of cytochrome P450 transcripts in Papilio polyxenes by linear and angular furanocoumarins. 1214961 The effect of growth at low temperature  ...  A dictionary model of mammalian xenobiotic meta- bolism. 1196810 Metabolism of estrogenic isoflavones in domestic animals. 1209646 Metabolism of pisatin stereoisomers by Ascochyta rabiei strains transformed  ... 

Page 3417 of Bibliography of Agriculture. Annual Cumulative Indexes Vol. 60, Issue CUMULATIVE INDEX [page]

Bibliography of Agriculture. Annual Cumulative Indexes  
characterization of chlorophenol 4-monooxy- genase from Burkholderia cepacia AC 1100. 1290336 Purification and properties of NAD(P)H: eure oxidoreductase of sugarbeet cells. 1280073 A quinol and steroids  ...  Effect of Royal Jelly on serum lipids in experimental animals and humans with atherosclerosis. 1256441 Effects of feeding Artemisia filifolia and Helenium flexuosum on rabbit cytochrome P450 isozymes.  ... 

ABSTRACTS 11th International Symposium on Chemistry and Biology of Pteridines and Folates

Wolfgang Pfleiderer, Konstanz Rokos, Hartmut Rokos
1997 Pteridines  
A well documented cytochrome P450 species is the soluble enzyme isolated from Pseudomonas putida (P45 0,,",,, indicating its substrate specifity for camphor).  ...  The exact mode of binding and the biological significance of pteridine derivatives as inhibitors for the wide spread cytochrome P450 enzyme system will be investigated in further studies.  ...  The significance of cytochrome P450 catalyzed reactions have been recognized as one of the most sophisticated methods to oxidize endogen-  ... 
doi:10.1515/pteridines.1997.8.2.103 fatcat:3xki34cbbbdina4nk6lmd3hroe

Survey of the 1999 surface plasmon resonance biosensor literature

Rebecca L. Rich, David G. Myszka
2000 Journal of Molecular Recognition  
The optical biosensor studies on the role of hydrophobic tails of NADPH- cytochrome P450 reductase and cytochromes P450 2B4 and b5 upon productive complex formation within a monomeric reconstituted system  ...  Optical biosensor studies on the productive complex formation between the components of cytochrome P450scc dependent monooxy- genase system. Biochem. Mol. Biol.  ... 
doi:10.1002/1099-1352(200011/12)13:6<388::aid-jmr516>3.0.co;2-# pmid:11114073 fatcat:rkzb7lafjneajipclizyjjd5la