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A GSH Fluorescent Probe with a Large Stokes Shift and Its Application in Living Cells

Mao, Xu, Li, Wang, Du, Liu, Ding, Liu
2019 Sensors  
Probe GP can detect intracellular effectively and shows obvious yellow fluorescence. Briefly, probe GP can detect intracellular GSH rapidly and effectively both in vitro and in living cells.  ...  The response time of GP to GSH was only 5 min and the product GO (the product of GP after reacting with GSH) after reacting with GSH possesses a larger Stokes shift for 135 nm than that in reported work  ...  ., H.D, and R.D. did the experiments. L.L. conducted the analysis of experiment results. Y.M. designed the experiment and wrote the paper. G.L. helped to analyze the data.  ... 
doi:10.3390/s19245348 pmid:31817200 pmcid:PMC6960701 fatcat:j62jpwtzwvdmdjwicheqy6enfm

A Green-emitting Fluorescent Probe Based on a Benzothiazole Derivative for Imaging Biothiols in Living Cells

Xiaohua Ma, Yuanqiang Hao, Jiaxiang Liu, Guoguang Wu, Lin Liu
2019 Molecules  
Probe 1 exhibited favorable properties such as excellent selectivity, highly sensitive (0.12 µM), large Stokes shift (117 nm) and a remarkable turn-on fluorescence signal (148-fold).  ...  Furthermore, confocal fluorescence imaging indicated that probe 1 was membrane-permeable and suitable for visualization of biothiols in living A549 cells.  ...  Due to its good photostability, large Stokes shift, good cell membrane permeability and low toxicity, 2-(benzothiazol-2-yl)phenol and its derivatives have been widely used for the design of fluorescent  ... 
doi:10.3390/molecules24030411 fatcat:dsomwio2kzexvd3ccu3qo7rcgy

Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator

Song Chen, Peng Hou, Jingwen Sun, Haijun Wang, Lei Liu
2019 Molecules  
Probe MIPY-DNBS exhibited a 172 nm Stokes shift, a fast response time (400 s), low cytotoxicity, low detection limits (12.7 nM for Cys), and excellent selectively in the detection of thiols.  ...  In addition, MIPY-DNBS was successfully applied to imaging thiols in living MCF-7 cells and zebrafish.  ...  As a consequence, it is of great significance to explore highly selective fluorescent probes with large stokes shifts for sensitively tracing biothiols in vivo.  ... 
doi:10.3390/molecules24183328 pmid:31547406 pmcid:PMC6767008 fatcat:hiyhyhsfarc7zfeu474pde4dju

Diethyl Malonate-Based Turn-On Chemical Probe for Detecting Hydrazine and Its Bio-Imaging and Environmental Applications With Large Stokes Shift

Jianbo Qu, Zhi-Hao Zhang, Haitao Zhang, Zhen-Tao Weng, Jian-Yong Wang
2021 Frontiers in Chemistry  
The novel probe NE-N2H4 exhibits good properties, such as large Stokes shift (about 125 nm), good selectivity, and low cytotoxicity.  ...  This sensing probe NE-N2H4 can be operated to detect hydrazine in living HeLa cells.  ...  Z-HZ and Z-TW: design, synthesize, characterize the dyes, write and edit manuscript, and manage the research project. All authors: contributed to the article and approved the submitted version.  ... 
doi:10.3389/fchem.2020.602125 pmid:33816431 pmcid:PMC8012553 fatcat:yr5z7edhs5e4tfpclhpitldpme

Emerging analytical tools for the detection of the third gasotransmitter H2S, A comprehensive review

Hany Ibrahim, Ahmed Serag, Mohamed A. Farag
2020 Journal of Advanced Research  
Advantages and limitations with regards to the application of each technique are highlighted with special emphasis on its employment for H2S in vivo measurement i.e., biofluids, tissues.  ...  Fluorescence methods applied for H2S measurement offer an attractive non-invasive and promising approach in addition to its selectivity, however they cannot be considered as H2S-specific probes.  ...  Farag thanks Jesour grant number 30, Academy of Scientific Research and Technology, ASRT, Egypt for funding.  ... 
doi:10.1016/j.jare.2020.05.018 pmid:33318873 pmcid:PMC7728591 fatcat:4o33qf6fsvejnjhomqj3sykcam

A Phenothiazine-HPQ Based Fluorescent Probe with a Large Stokes Shift for Sensing Biothiols in Living Systems

Yan Zheng, Peng Hou, Yu Li, Jingwen Sun, Hongxia Cui, Haiyan Zhang, Song Chen
2021 Molecules  
Probe PHPQ-SH exhibited brilliant sensing performances toward thiols, including a large Stokes shift (138 nm), excellent sensitivity (for GSH, LOD = 18.3 nM), remarkable fluorescence enhancement (163-fold  ...  In this work, we reported an efficient fluorescent probe, PHPQ-SH, for detecting biothiols in vitro and vivo, based on the phenothiazine-HPQ skeleton, with DNBS (2,4-dinitrobenzenesulfonate) as the response  ...  Bu and H. J. Wang for generous support and constant encouragement. Conflicts of Interest: The authors declare that they have no conflicts of interest.  ... 
doi:10.3390/molecules26082337 pmid:33920567 fatcat:66viy4z2m5co3nbvh5r7dfixfe

Lysosome-Targeted Single Fluorescence Probe for Two-Channel Imaging Intracellular SO2 and Biothiols

Yue Wang, Li Liu, Xian-Li Zhou, Ming-Yu Wu
2019 Molecules  
BPO-DNSP can sensitively and selectively respond to SO2 in the green channel with a large Stokes shift over 105 nm, and to biothiols in the near-infrared emission channel with a large Stokes shift over  ...  Here, a lysosome-targeted fluorescent probe (BPO-DNSP) with a dual reaction site for SO2 and biothiols was presented.  ...  However, BPO-DNSP can sensitively and selectively respond to SO 2 and emit green fluorescence with a large Stokes shift over 105 nm, and it can react with biothiols in the near-infrared emission channel  ... 
doi:10.3390/molecules24030618 fatcat:qftkeexexjh6pbkzhxk5sdz6yu

Challenges and Opportunities for Small-Molecule Fluorescent Probes in Redox Biology Applications

Xiqian Jiang, Lingfei Wang, Shaina L. Carroll, Jianwei Chen, Meng C. Wang, Jin Wang
2018 Antioxidants and Redox Signaling  
We specifically highlight the advantages of reversibility in sensing reactions and its applications in ratiometric probe design for quantitative measurements in living cells.  ...  Small-molecule fluorescent probes have been widely used to detect and/or quantify ROS/ RNS in many redox biology studies and serve as an important complementary to protein-based sensors with unique applications  ...  Acknowledgments The research was supported in part by the National Institutes of Health (R01-GM115622, R01-CA207701, R21-CA213535 to J.W., R01-AG045183, R01-AT009050, R21-EB022302, and DP1-DK113644 to  ... 
doi:10.1089/ars.2017.7491 pmid:29320869 pmcid:PMC6056262 fatcat:oqfm66bsonf5vjdh5s7z4ui7oa

An effective colorimetric and ratiometric fluorescent probe based FRET with a large Stokes shift for bisulfite

Wen-Li Wu, Zhao-Yang Wang, Xi Dai, Jun-Ying Miao, Bao-Xiang Zhao
2016 Scientific Reports  
In this study, we report a colorimetric and ratiometric fluorescent probe (CPT) with a large Stokes shift (162 nm) for bisulfite (HSO 3 − ) based FRET mechanism.  ...  Moreover, probe CPT was used to ratiometric fluorescent imaging of exogenous and endogenous HSO 3 − in living cells.  ...  Acknowledgements This study was supported by the Natural Science Foundation of Shandong Province (ZR2014BM004) and Major Project of Science and Technology of Shandong Province (NO. 2015ZDJS04001 and 2015ZDJS04003  ... 
doi:10.1038/srep25315 pmid:27137791 pmcid:PMC4853739 fatcat:cw5k2fsscjgtrdr4yvci6hjfzq

ESIPT Fluorescence Probe Based on Double-Switch Recognition Mechanism for Selective and Rapid Detection of Hydrogen Sulfide in Living Cells

Hongwei Guan, Aixia Zhang, Peng Li, Lixin Xia, Feng Guo
2019 ACS Omega  
A novel fluorescence probe, HBTSeSe, was designed and synthesized for the detection of H2S with a double-switch mechanism of a broken diselenide bond followed by thiolysis of ether.  ...  The probe responded selectively and rapidly to H2S, with the fluorescence increased by 47-fold immediately after the addition of H2S.  ...  Herein, we report the design, synthesis, and biological applications of a fluorescence probe with selective and rapid response toward H 2 S, and a large Stokes shift is achieved.  ... 
doi:10.1021/acsomega.9b00934 pmid:31459999 pmcid:PMC6648457 fatcat:vcfcw6j46renriazdxbj7f5pyi

Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide

Tianbao Liu, Jie Lin, Zhen Li, Lin Lin, Yuning Shen, Hailiang Zhu, Yong Qian
2015 The Analyst  
We have developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive imaging of H2S in living cells and living zebrafish in vivo.  ...  Thus, ratiometric fluorescent probes with good 70 solubility, large Stokes shift, longer excitation wavelength, and high quantum yield are still urgently needed for hydrogen sulfide imaging in living cells  ...  This probe exhibited a remarkably fluorescence response to hydrogen sulfide over other physiological thiols or biological species, which fluoresces in the red region with a large Stokes shift (172 nm).  ... 
doi:10.1039/c5an00119f pmid:26401525 fatcat:pzvoxrz4k5c7damobsfanot7km

Development of Photo- and Chemo-stable Near-Infrared-Emitting Dyes: Linear-Shape Benzo-Rosol and Its Derivatives as Unique Ratiometric Bioimaging Platforms

Mingchong Dai, Ye Jin Reo, Chang Wook Song, Yun Jae Yang, Kyo Han Ahn
2020 Chemical Science  
Microscopic imaging aided with fluorescent probes has revolutionized our understanding on biological systems. Organic fluorophores and probes thus continue to evolve for bioimaging application.  ...  Fluorophores such as cyanines and hemicyanines...  ...  Introduction Microscopic imaging aided with a fluorescent probe is essential for studying biological processes in living systems.  ... 
doi:10.1039/d0sc03314f pmid:34123144 pmcid:PMC8163444 fatcat:ubawvzlnlralvbxbvzejnvqrfy

A pyrene-based ratiometric fluorescent probe with a large Stokes shift for selective detection of hydrogen peroxide in living cells

Qingxin Chen, Ke Cheng, Wanhe Wang, Liu Yang, Yusheng Xie, Ling Feng, Jie Zhang, Huatang Zhang, Hongyan Sun
2020 Journal of Pharmaceutical Analysis  
In this work, we reported a new pyrene-based compound Py-VPB as H2O2 fluorescent probe in vitro. The probe demonstrated ratiometric detection behavior, large Stokes shift and large emission shift.  ...  Although numerous fluorescent imaging probes for H2O2 have been reported, the development of H2O2 ratiometric fluorescent probe with large Stokes shift remains rather limited.  ...  These experimental data demonstrated that Py-VPB is a potential ratiometric fluorescent probe with large Stokes shift and emission shift as we expected.  ... 
doi:10.1016/j.jpha.2020.07.003 pmid:33133733 pmcid:PMC7591780 fatcat:4sroixjemfbk5cq7pdeuht43ze

A 7-Hydroxybenzoxazinone-Containing Fluorescence Turn-On Probe for Biothiols and Its Bioimaging Applications

Bin Li, Datong Zhang, Ruibing An, Yaling Zhu
2019 Molecules  
Upon treatment with biothiols, PBD shows a strong fluorescence enhancement (up to 70-fold) and a large Stokes shift (155 nm).  ...  Finally, the potential utility of this probe for biothiol sensing in living HeLa cells is demonstrated.  ...  We prepared 7-hydroxy-3-phenylbenzoxazinone (PBOH) and evaluated its optical property. PBOH exhibits strong yellow-green fluorescence and a large Stokes shift (155 nm).  ... 
doi:10.3390/molecules24173102 pmid:31461829 pmcid:PMC6749190 fatcat:sruccyop7fhddphven6b6tixhm

A fast-responsive two-photon fluorescent probe for monitoring endogenous HClO with a large turn-on signal and its application in zebrafish imaging

Jian-Yong Wang, Jianbo Qu, Haitao Zhang, Kang Wei, Shan-Xiu Ni
2019 RSC Advances  
Fast-responsive two-photon fluorescent probe NS-ClO for imaging endogenous HClO in vivo with a large turn-on signal (about 860 times) and Stokes shift (about 90 nm), fast response (about 1 min) and good  ...  Notes and references  ...  Therefore, it is worth to develop a two-photon uorescent probe for imaging HClO specically in vivo with a large Stokes shi and a turn-on signal.  ... 
doi:10.1039/c9ra02160d pmid:35516359 pmcid:PMC9064391 fatcat:bs7xgyqerfeebdteyl27oibxci
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