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siRNA gelsolin knockdown induces epithelial-mesenchymal transition with a cadherin switch in human mammary epithelial cells

Hiroki Tanaka, Reza Shirkoohi, Koji Nakagawa, Hongjiang Qiao, Hisakazu Fujita, Futoshi Okada, Jun-ichi Hamada, Satoshi Kuzumaki, Masato Takimoto, Noboru Kuzumaki
2006 International Journal of Cancer  
Kwiatkowski (Harvard Medical School, Boston) .  ...  A. gelsolin overexpression clones; wild-type of MCF10A (WT), Neo-transfected (Neo-10A c1 and c2), ectopic human gelsolin-overexpressed (Gsn- Figure 2 . 2 Effect of gelsolin siRNAs on morphological changes  ... 
doi:10.1002/ijc.21559 pmid:16217750 fatcat:umvdoneq2nc5pei7tp6pwpwtym

Secreted gelsolin desensitizes and induces apoptosis of infiltrated lymphocytes in prostate cancer

Chun-Chi Chen, Shiow-Her Chiou, Cheng-Lin Yang, Kuan-Chih Chow, Tze-Yi Lin, Hui-Wen Chang, Weir-Chiang You, Hisu-Wen Huang, Chien-Min Chen, Nien-Cheng Chen, Fen-Pi Chou, Ming-Chih Chou
2017 OncoTarget  
Target proteins were visualized through the exposure of the membrane to an X-Omat film (Eastman Kodak, Rochester, NY, USA) with enhanced chemiluminescent reagent (NEN, Boston, MA, USA).  ...  The protein was visualized through the exposure of the membrane to an X-Omat film (Eastman Kodak, Rochester, NY) with enhanced chemiluminescent reagent (NEN, Boston, MA).  ... 
doi:10.18632/oncotarget.20414 pmid:29100377 pmcid:PMC5652770 fatcat:ekdighwo5fdz3hqnlfmly4rbbi

CREB activation induced by mitochondrial dysfunction triggers triglyceride accumulation in 3T3-L1 preadipocytes

S. Vankoningsloo
2006 Journal of Cell Science  
Greenberg (Harvard Medical School, Boston, MA, USA) for the constructs encoding dominant negative forms of CREB (K-CREB and M1- Fig. 1 . 1 Antimycin A and chloramphenicol induce triglyceride accumulation  ...  , Coll VI␣2, CPT-2, FAS, GPDcyt, Gsn, Hp, MMP-2, PPAR␥, resistin, RXR␣, SCD1, SREBP-1, Journal of Cell Science 119 (7) UCP-2), begin to be upregulated after 4 days (Acrp30, adipsin, Cav-2, C/EBP␣, Clic4  ... 
doi:10.1242/jcs.02848 pmid:16537646 fatcat:3n5pczprvjaytippwlu5ndn3g4

Elephant brain

Jeheskel Shoshani, William J. Kupsky, Gary H. Marchant
2006 Brain Research Bulletin  
Only 1/2 of cerebellum available (the other half brain is with Darlene Ketten, Harvard Medical School, Boston, MA, USA), the 960 g is a result of 480 g × 2. , includes data, as composite from different  ...  cm 2 ) and human (170.67 cm 2 ).  ... 
doi:10.1016/j.brainresbull.2006.03.016 pmid:16782503 fatcat:sewwe6wa3bffncmanq4xbsu5oa

Proteomic Analyses of Mammary Glands Provide Insight into the Immunity and Metabolism Pathways Associated with Clinical Mastitis in Meat Sheep

Gao, Li, Lu, Wang, Zhao, Ma
2019 Animals  
The 2-DE results showed that a total of 10 up-regulated and 16 down-regulated proteins were identified in CMMTs when compared to HMTs.  ...  For a better understanding of the disease-associated proteins during clinical mastitis in meat sheep, we performed two-dimensional electrophoresis (2-DE)-based comparative proteomic analyses of mammary  ...  Protein Identification and Functional Enrichment Analysis Proteins were identified using Mascot peptide mass fingerprinting software 2.2 (Matrix Science, Boston, MA, USA) to search against the NCBI non-redundant  ... 
doi:10.3390/ani9060309 pmid:31159303 pmcid:PMC6617192 fatcat:a35bvdktczhpjpw246wmrjefi4

Proceedings of CollECTeR Europe 2006 [article]

Petra Schubert, Daniel Risch, Fachhochschule Nordwestschweiz FHNW, Fachhochschule Nordwestschweiz FHNW
2015
Conference Topic 2006: Collaborative BusinessThe ?networked economy?  ...  This constitutes our notion of Collaborative Business: the timely bundling of communication, coordination, and collaboration activities.The focus of CollECTeR Europe 2006 is on new forms of Customer Relationship  ...  GSN/WAP-based banking has been a cornerstone of Finnish Mobile phone applications for a long time.  ... 
doi:10.26041/fhnw-3081 fatcat:x7eqljzuvfbodi7gdmdacqbwem

An investigation of proposed techniques for quantifying confidence in assurance arguments

Patrick J. Graydon, C. Michael Holloway
2017 Safety Science  
Proceedings of the 31st International System Safety Conference (ISSC), Boston, MA, USA, August 2013. URL http://www.mrtc.mdh.se/ index.php?choice=publications&id=3349. 10.  ...  Government Accountability O ce, Washington, DC, USA, April 2012. URL http://www.gao.gov/ assets/590/589825.pdf. 13. Cullen, W. D.: The Public Inquiry Into the Piper Alpha Disaster , vol. 2.  ...  L.2 Replication We had little di culty reproducing the example.  ... 
doi:10.1016/j.ssci.2016.09.014 fatcat:c3adi6biyjgitfiwbvxucrdi7y

Proteomic changes of the porcine small intestine in response to chronic heat stress

Yanjun Cui, Xianhong Gu
2015 Journal of Molecular Endocrinology  
The antigen-antibody interaction was carried out for 1 h, and the cross-reacting proteins were detected using ECL (Perkin Elmer Life Sciences, Boston, MA, USA).  ...  , GSN 6.7E-3 8.2E-2 Downloaded from Bioscientifica.com at 04/28/2019 07:41:17AM via free access  ... 
doi:10.1530/jme-15-0161 pmid:26416815 pmcid:PMC4632496 fatcat:vqcqke33z5huraz3v2c3lctiwi

Nuclear colocalization and interaction between bcl-xL and cdk1(cdc2) during G2/M cell-cycle checkpoint

E Schmitt, M Beauchemin, R Bertrand
2007 Oncogene  
Here, we show that in addition to its mitochondrial effect that delays apoptosis, bcl-xL colocalizes and binds to cdk1(cdc2) during G 2 /M cellcycle checkpoint and its overexpression stabilizes a G 2 /  ...  A mutant deleted of this region does not alter the antiapoptotic function of bcl-xL, but impedes its effect on cdk1(cdc2) activity and on the G 2 /M-arrest senescence program after DNA damage.  ...  ; AF800, R&D System, Minneapolis, MN, USA); anti-cytochrome c (clone 7H8.2C12, PharMingen, Mississauga, ON, Canada), anti cdk1(cdc2) (Ab-1, Oncogene Research Products, Boston, MA, USA; clone 17, SantaCruz  ... 
doi:10.1038/sj.onc.1210396 pmid:17369848 fatcat:rvj3lepdp5dllkttyhfvh5q45a

Glutathione depletion triggers actin cytoskeleton changes via actin-binding proteins

Nahum Zepeta-Flores, Mahara Valverde, Alejandro Lopez-Saavedra, Emilio Rojas
2018 Genetics and Molecular Biology  
Data analysis Image quantification, background subtraction and scaling were carried out with dChip software (Harvard, Boston, MA, USA) with 100% recall between control and lower GSH level chips and p<0.05  ...  San Diego, CA, USA) as the probe. When DHR is oxidized by H 2 O 2 in the presence of peroxidases, it produces the fluorescent compound rhodamine 123.  ... 
doi:10.1590/1678-4685-gmb-2017-0158 pmid:29870570 pmcid:PMC6082235 fatcat:ul4u462sfnarpbooexrpqfds3e

Plasma profiles in active systemic juvenile idiopathic arthritis: Biomarkers and biological implications

Xuefeng B. Ling, Jane L. Park, Tanya Carroll, Khoa D. Nguyen, Kenneth Lau, Claudia Macaubas, Edward Chen, Tzielan Lee, Christy Sandborg, Diana Milojevic, John T. Kanegaye, Susanna Gao (+3 others)
2010 Proteomics  
, San Francisco (UCSF) from 2006 to 2008.  ...  , MA) search engine.  ... 
doi:10.1002/pmic.201000298 pmid:21136595 pmcid:PMC3517169 fatcat:fedacynp6zcqrp7vll46utprwq

Proteomic analysis of multiple primary cilia reveals a novel mode of ciliary development in mammals

K. Narita, H. Kozuka-Hata, Y. Nonami, H. Ao-Kondo, T. Suzuki, H. Nakamura, K. Yamakawa, M. Oyama, T. Inoue, S. Takeda
2012 Biology Open  
Notably, ultrastructural analysis revealed the presence of not only 9+0 but also 9+2 and atypical ciliary subtypes in neonatal CPEC.  ...  Live imaging of mouse choroid plexus revealed that neonatal CPEC cilia could beat vigorously, and the motility waned and was lost within 1-2 weeks.  ...  , USA).  ... 
doi:10.1242/bio.20121081 pmid:23213475 pmcid:PMC3507226 fatcat:lzjov6d5izhyfeefoysivir3aa

Circulating Exosomes Are Strongly Involved in SARS-CoV-2 Infection

Elettra Barberis, Virginia V. Vanella, Marco Falasca, Valeria Caneapero, Giuseppe Cappellano, Davide Raineri, Marco Ghirimoldi, Veronica De Giorgis, Chiara Puricelli, Rosanna Vaschetto, Pier Paolo Sainaghi, Stefania Bruno (+8 others)
2021 Frontiers in Molecular Biosciences  
The present study used a shotgun proteomic approach to map the host circulating exosomes' response to SARS-CoV-2 infection.  ...  Knowledge of the host response to the novel coronavirus SARS-CoV-2 remains limited, hindering the understanding of COVID-19 pathogenesis and the development of therapeutic strategies.  ...  Louis, MO, USA) according to the manufacturer's procedure.  ... 
doi:10.3389/fmolb.2021.632290 pmid:33693030 pmcid:PMC7937875 fatcat:kofxufrfp5dyzn5yzjy6e2qrja

Molecular profiling of innate immune response mechanisms in ventilator-associated pneumonia [article]

KHYATIBEN V PATHAK, Marissa I McGilvrey, Charles K Hu, Krystine Garcia-Mansfield, Karen Lewandoski, Zahra Eftekhari, Yate-Ching Yuan, Frederic Zenhausern, Emmanuel Menashi, Patrick Pirrotte
2020 bioRxiv   pre-print
Raw data were searched with 160 the Mascot search engine (MatrixScience, Boston, MA) against a recent human database (2015) 161 177 quantify 185 metabolites.  ...  Clin Infect Dis 51 Suppl 1, S12-17 457 2. Koenig, S. M., and Truwit, J. D. (2006) Ventilator-associated pneumonia: diagnosis, 458 treatment, and prevention. Clin Microbiol Rev 19, 637-657 459 3.  ... 
doi:10.1101/2020.01.08.899294 fatcat:427rwii4ujf2lbhjwd7bbgajbq

Abstracts of the 6th Cachexia Conference, Milan, Italy, December 8-10, 2011

2011 Journal of Cachexia, Sarcopenia and Muscle  
In this study, we investigated the effects of leucine on cell viability, morphology, functional proteasome activity, enzymatic activities, and protein synthesis and degradation in C 2 C 12 skeletal muscle  ...  Taken together, these results suggested an important modulatory effect of leucine under the WF actions in C 2 C 12 myotube cells. 1-02 Habitual skeletal muscle protein fractional synthetic rate in healthy  ...  , Filippo Rossi Fanelli 2 , Per-Olof Hasselgren 1 ( 1 Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; 2 Department of Clinical Medicine, Sapienza  ... 
doi:10.1007/s13539-011-0045-3 pmid:22476871 pmcid:PMC3222823 fatcat:yjup7zdkwvhcjd6h455t77av4a
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