UV-visible derivative spectroscopic method for simultaneous estimation of cefoperazone and tazobactam in injection dosage form release_zqe672fgabhn3japzyvsytwjqe

Abstract

A simple, accurate, rapid, economical UV spectrophotometric method namely Absorption ratio and RP-HPLC method have been developed and validated for estimation of Cefoperazone and Tazobactam in injection dosage form and can be used in routine analysis. Cefoperazone is 3rd generation of cephalosporin containg drug used in Septicemia, Biliary tract infection and urinary tract infection and Tazobactam is a Antibacterial agent used along with cefoperazone in emergency. It is manufactured by Biocon Pharmaceuticals (New Delhi) under the brand name Cegava-Tz. The ZCPs for CEFO and TAZO were found to be 320 nm and 232.8 nm respectively. Results of the validation of the above method indicate that the method was linear in the range of 25-75 µg/ml for CEFO and 3.125-9.75 µg/ml for TAZO. The % recoveries for CEFO and TAZO obtained in the accuracy study were 97.43-98.82 % and 95.46-100.2% respectively. The high precision of proposed method is confirmed by % RSD below 2.0 for repeatability. The % assay results of 97.84 % for CEFO and 100.25 % for TAZO indicate the proposed derivative spectroscopic method was simple, accurate, pricise and successfully applied for determination of CEFO and TAZO from Cegava-Tz injection dosage form forroutine analysis.
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