<i>In vitro</i> anti-PRV activity of dihydromyricetin from <i>Ampelopsis grossedentata</i> release_yetl4dwhvfcdzcgpa2dsix6dly

Abstract

Pseudorabies (PR) is an acute infectious disease caused by pseudorabies virus (PRV). There are no available drugs due to the emergence of variant of PRV. Dihydromyricetin (DMY) is a flavonoid extracted from <i>Ampelopsis grossedentata</i> (<i>A. grossedentata</i>), which has a variety of pharmacological activities. In this study, we aim to investigate the <i>in vitro</i> anti-PRV activity of DMY extracted from <i>A. grossedentata</i>. MTT assay was used to detect the cytotoxicity and antiviral activity of DMY. The results detected by flow cytometry and qRT-PCR showed that DMY played anti-PRV role mainly by interfering with the process of virus invasion into host cell and inhibiting the occurrence of pyroptosis <i>in vitro.</i> This suggested that anti-pyroptosis may be an important antiviral mechanism for DMY which is expected to be a potential anti-PRV drug.
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