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Study of P53 gene mutations in promoter and exons 2-4 and 9-11 in patient with gastric cancer by PCR-SSCP in Chaharmahal Va Bakhtiari province
release_wvtem73z4jahdnadimsqu6nva4
by
Javad Saffari Chaleshtary,
Mohammad taghi Moradi,
Effat Farrokhi,
Mohammad Amin Tabatabaiefar,
Maryam taherzadeh,
Fatemeh Azadegan,
Gholamreza Mobini,
Mehdi Banitalebi,
Ghorban Ali Rahimian,
Morteza Hashemzadeh Chaleshtary
Published
in Iranian South Medical Journal
by Bushehr University of Medical Sciences.
2011 Volume 14, p220-229
Abstract
Background: Gastric cancer is one of the most important diseases and after lung cancer, is the second cause of cancer death worldwide. Genetic factors including oncogenes and tumor suppressor genes are always contributed in progression of this cancer. The P53 tumor suppressor gene has a broad role in genomic stability and DNA repair. The aim of this study was to determine the P53 gene mutations in gastric cancer specimens in Chaharmahal Va Bakhtiari Province. Methods: In this descriptive-lab based study, we investigated the promoter, exons 2-4 and 9-11 of P53 gene mutations in 38 paraffin embedded gastric cancer specimens. DNA was extracted following the standard phenol chloroform protocol. The P53 gene mutations were determined using PCR-SSCP procedure. Results: Our study revealed no P53 gene mutation in promoter and exons 2-4 and 9-11 in the gastric cancer subjects studied. Conclusion: While P53 gene mutations have been reported as the most frequent genetic alterations and are found in about 50% of the human malignancies, no mutation was detected in this study. The reason may be due to small sample size or mutations on other genes or epigenetic factors.
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