Molecular detection of Coxiella burnetii in human and sheep in Al-Diwaniyah province by Real Time-PCR ‫بأستخدام‬ ‫الديوانية‬ ‫محافظة‬ ‫في‬ ‫واألغنام‬ ‫اإلنسان‬ ‫في‬ ‫البيورنتية‬ ‫لاللكوكسيال‬ ‫الجزيئي‬ ‫الكشف‬ ‫تقني‬ .‫الحقيقي‬ ‫الوقت‬ ‫في‬ ‫البلمرة‬ ‫سلسلة‬ ‫تفاعل‬ ‫ة‬ ‫الحمداوي‬ ‫مايع‬ ‫قايد‬ ‫ختام‬ release_wehyenjlqfhrbckzibp65twfkm

Abstract

Background: Iraq is agricultural country, but the important aspect of livestock shown by most of the studies conducted in previous years, which dealt with Q fever infection in animals (cattle, sheep and goat) strikingly. Q fever it is a worldwide zoonotic disease caused by Coxiella burentii. Material and methods: One hundred and five (105), human samples used in the study were (50) (22 sputum/28 bronchial-alveolar lavage) collected from Teaching Hospital and Center Pulmonology and Respiratory Specialist in Al-Diwaniyah. Collected sheep samples were (55) Sample (28 nasopharyngeal swabs and 27 bronchial fluids) from the slaughterhouse of Al-Diwaniya during January-august 2016. All samples were collected from peoples & sheep that suffer from clear respiratory signs. By using Sybr Green Real Time-PCR technique the samples tested directly. Results: showed that the infection incidence in humans (16%) and in sheep (38.181%), the study proved that there were a significant differences at the site of infection in the respiratory tract (URT& LRT) for humans and sheep alike, as well as in terms of age groups and gender. Conclusions: Q fever is a serious disease circulating between peoples in Al-Diwaniyah city and clearly defined risk groups: people with respiratory infection, heart and vascular disease, immunosuppressed patients and distributed in sheep that have respiratory disorder. Real Time-PCR is a potential tool to diagnosis of C. burnetii in humans and animals, because of its rapid and sensitive detection. 391 Coxiella burentii ‫لحمى‬ ‫المسبب‬ ‫العامل‬ ‫هي‬ Q ‫ذو‬ ‫و‬ ‫والحيوانات‬ ‫البشر‬ ‫بين‬ ‫ينتقل‬ ‫المنشأ‬ ‫حيواني‬ ‫مرض‬ ‫هو‬ ‫،و‬ .‫عالمي‬ ‫انتشار‬ ‫العمل‬ ‫وطرق‬ ‫المواد‬ ‫ا‬ ‫من‬ ‫عينات‬ ‫جمع‬ ‫تم‬ : ‫ألشخاص‬ ‫التنفسي‬ ‫الجهاز‬ ‫في‬ ‫واضحة‬ ‫عالمات‬ ‫من‬ ‫تعاني‬ ‫التي‬ ‫واألغنام‬. (‫وخمسة‬ ‫مائة‬ ‫وكانت‬ 501 ‫اس‬ ‫التي‬ ‫البشرية‬ ‫العينات‬) (‫الدراسة‬ ‫في‬ ‫تخدمت‬ 10 () 22 / ‫بلغم‬ 22 ‫الغسل‬ ‫سوائل‬ ‫الديوانية‬ ‫في‬ ‫التخصصي‬ ‫والتنفسية‬ ‫الصدرية‬ ‫االمراض‬ ‫ومركز‬ ‫التعليمي‬ ‫المستشفى‬ ‫من‬ ‫جمعها‬ ‫تم‬ ‫التي‬) ‫القصبي‬ (‫األغنام‬ ‫من‬ ‫جمعها‬ ‫تم‬ ‫التي‬ ‫العينات‬ ‫وكانت‬ 11 (‫عينة‬) 22 ‫و‬ ‫البلعوم‬ ‫مسحات‬ 22 ‫في‬)‫الهوائية‬ ‫الشعب‬ ‫السوائل‬ ‫الديوا‬ ‫مجزرة‬ ‫يناير‬ ‫خالل‬ ‫نية‬-‫أغسطس‬ 2052. ‫النتائج‬ (‫البشر‬ ‫بين‬ ‫عدوى‬ ‫حاالت‬ ‫أن‬ ‫أظهرت‬ : 52 (‫األغنام‬ ‫في‬ ‫و‬)٪ 12.525 ‫هناك‬ ‫أن‬ ‫الدراسة‬ ‫أثبتت‬ ‫وقد‬ ،)٪ ‫التنفسي‬ ‫الجهاز‬ ‫في‬ ‫اإلصابة‬ ‫موقع‬ ‫في‬ ‫كبيرة‬ ‫اختالفات‬ ‫فروق‬ ‫الدراسة‬ ‫اوجدت‬ ‫كما‬ ،‫سواء‬ ‫حد‬ ‫على‬ ‫والغنم‬ ‫للبشر‬ ‫والج‬ ‫العمرية‬ ‫الفئات‬ ‫حيث‬ ‫من‬ ‫معنوية‬ ‫نس‬. ‫االستنتاجات‬ ‫حمى‬ : Q ‫واضح‬ ‫للخطر‬ ‫المعرضة‬ ‫,الفئات‬ ‫الديوانية‬ ‫مدينة‬ ‫في‬ ‫االشخاص‬ ‫بين‬ ‫دائرا‬ ‫يزال‬ ‫ال‬ ‫خطير‬ ‫مرض‬ ‫هو‬ ‫يعانون‬ ‫الذين‬ ‫والمرضى‬ ،‫الدموية‬ ‫واألوعية‬ ‫القلب‬ ‫وأمراض‬ ‫التنفسي‬ ‫الجهاز‬ ‫التهابات‬ ‫من‬ ‫يعانون‬ ‫الذين‬ ‫األشخاص‬ : ‫هم‬ ‫المعالم‬ ‫لديها‬ ‫التي‬ ‫األغنام‬ ‫في‬ ‫و‬ ‫المناعة‬ ‫كبت‬ ‫ال‬ ‫تقنية‬ ‫تعد‬ ‫التنفسي.و‬ ‫الجهاز‬ ‫في‬ ‫اضطراب‬ real time pcr ‫التشخيص‬ ‫في‬ ‫معتمدة‬ ‫تقنية‬ ‫لبكتريا‬ ‫حساسيتها‬ ‫و‬ ‫لسرعتها‬ C. burnetti .
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