@article{arend_2016,
title={Early ovariectomy unmasking the non-somatic origin of murine anti-A reactive IgM},
DOI={10.6084/m9.figshare.1279394.v108},
abstractNote={The germline encoding of a non-immune IgM
molecule in mammals was experimentally documented for the first time by
specifically timed ovariectomy of C57BL/10 mice. Although ovariectomy and
castrations have been described to result in uncontrolled and/or enhanced
humoral and cellular immunity, involving increasing weights of spleen and
thymus with pronounced B and T cell productions, the development of the
mercaptoethanol-sensitive and complement-binding, non-immune anti-A reactivity
in murine plasma was not enhanced after ovariectomy performed in C57BL/10 mice
before the onset of puberty. This non-immune murine anti-A, which is
complementary to trans-species, syngeneic ovarian GalNAc-glycan-bearing glycolipids
and distinct from cross-reactive adaptive anti-A antibody, was strongly retarded
or did not appear at all in plasmas of animals ovariectomized at the age of 20
days. Thus, contrary to our previous view, this reactivity is unlikely to
originate from a somatic, primary immune or autoimmune response. All murine
tissues expressed the species-typical Forssman
reactivity and further A-like structures were, by innate human anti-A antibody,
identified in male and female reproductive and endodermal organs, while the murine
anti-A was exclusively inhibited by syngeneic ovarian glycolipids. Moreover,
the early ovarian tissue, which represents a last evolutionary and/or developmental location, showed
a developmental polymorphism characterized by DBL and HPA reactivity
indicating the involvement of O-GalNAc-determined
mucin-type A-like Tn and TF epitopes that, when expressed by non-developmental tissues, signify malignancy.},
publisher={Figshare},
author={Arend, Peter},
year={2016},
month={Aug}
}